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Effect of Difference Incubation Temperature on The Hatching Time and Hatching Rate of Sinodontis (Synodontis eupterus) Eggs Pangreksa, Amelia; Mustahal, Mustahal; Indaryanto, Forcep Rio; Nur, Bastiar
Jurnal Perikanan dan Kelautan Vol 6, No 2 (2016)
Publisher : JURNAL PERIKANAN DAN KELAUTAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (861.531 KB)

Abstract

Sinodontis is one of the ornamental fish that have high economic value, because of its unique behavior which could swim in reversed position. The purpose of this study was to determine whether there are differences in the effect of incubation temperature on hatching time and hatching time of sinodontis eggs, and to know the optimal temperature for the time of hatching time and hatching rate of the sinodontis eggs. The main parameters measured were the hatching time and hatching rate of sinodontis eggs. This experiment used completely randomized design with 4 treatment temperature difference, A (25-26oC), B (28-29oC), C (31-32oC) and D (control), with 3 replications. The results showed that the fastest hatching time of sinodontis obtained in treatment C at 1120 minutes (18 hours 40 minutes), then treatment B at 1240 minutes (20 hours 40 minutes), then treatment D at 1340 minutes (22 hours 20 minutes) and longest hatching time in treatment A at 1760 minutes (29 hours 20 minutes). Highest hatching rate of sinodontis obtained in A treatment that was equal to 94.67%, then C treatment that was equal to 82.67%, and treatment D amounted to 81.33% and lowest hatching rate in treatment B 80.00%. Based on the results of the study showed that the optimal temperature to accelerate the sinodontis for hatching time was at a temperature of 31-32oC, while the optimum temperature for increasing the hatchability of sinodontis eggs was at a temperature of 25-26°C.
INDUKSI PEMATANGAN GONAD IKAN GURAMI COKELAT (Sphaerichthys osphromenoides Canestrini, 1860) MENGGUNAKAN PREGNANT MARE SERUM GONADOTROPIN DAN ANTIDOPAMIN Nur, Bastiar; Cindelaras, Sawung; Meilisza, Nina
Jurnal Riset Akuakultur Vol 12, No 1 (2017): (Maret 2017)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1292.715 KB) | DOI: 10.15578/jra.12.1.2017.69-76

Abstract

Ikan gurami cokelat (Sphaerichthys osphromenoides Canestrini, 1860) merupakan salah satu spesies ikan hias endemik perairan gambut dan memiliki potensi untuk dibudidayakan. Pada kondisi budidaya, berbagai faktor lingkungan yang berperan penting dalam menstimulasi perkembangan dan pematangan gonad hingga ovulasi dan pemijahan tidak mendukung aktivitas reproduksi beberapa spesies ikan. Penelitian ini dilakukan untuk menentukan dosis hormon yang efektif dapat merangsang pematangan gonad ikan gurami cokelat. Hormon yang digunakan adalah “Oodev®” (tersusun atas Pregnant Mare Serum Gonadotropin/PMSG) dan antidopamin) yang diberikan menggunakan metode “topical gill”. Ada tiga dosis Oodev® yang digunakan, yaitu: 0,02 mL; 0,04 mL; dan 0,06 mL; serta kontrol menggunakan NaCl 0,9% sebanyak 0,05 mL/g bobot badan ikan uji. Setiap perlakuan menggunakan 30 ekor induk betina ikan gurami cokelat (panjang total: 4,1 ± 0,3 cm; bobot: 1,41 ± 0,17 g). Pemberian hormon dilakukan setiap minggu hingga minggu ke-7. Pada minggu ke-8 dilakukan pembedahan untuk pengambilan gonad. Parameter yang diamati adalah: jumlah induk matang gonad, indeks gonadosomatik (IGS), fekunditas, diameter oosit, kadar estradiol-17â dalam darah, dan tingkat kematangan gonad (TKG) ikan uji pada masing-masing perlakuan. Hasil penelitian menunjukkan bahwa perlakuan hormon dengan dosis 0,04 mL/g bobot badan menghasilkan perkembangan gonad ikan uji yang lebih baik dengan jumlah induk matang gonad mencapai 23 ekor (76,67%), nilai IGS sebesar 2,33 ± 1,24%; fekunditas sebesar 73,5 ± 26,2 butir; diameter oosit berkisar antara 1,0-1,8 mm; kadar estradiol-17b dalam darah sebesar 15,9 ± 4,5 rg/mL dengan tingkat kematangan gonad mencapai tahap IV.Chocolate gourami (Sphaerichthys osphromenoides Canestrini, 1860) is an endemic ornamental fish species in peatland waters and potentially to be cultivated. In captive condition, some environmental factors that play important role for gonadal development, maturation, ovulation, and spawning are not suitable for supporting reproductive activity in some fish species. This study was conducted to determine the optimum dosages of hormone which is able to stimulate gonadal maturation of chocolate gourami. Oodev® (consisted of Pregnant Mare Serum Gonadotropin (PMSG) and antidopamin) was given using “topical gill” method. There were three Oodev® dosages used in this research: 0.02 mL; 0.04 mL; and 0.06 mL respectively; and control using 0.05 mL of 0.9% NaCl per gram of body weight. Each treatment was tested on 30 females (the averages of total length and body weight of 4.1 ± 0.3 cm and 1.41 ± 0.17 g, respectively). Hormone was given every week until the seventh week. At the eight weeks, surgery was performed for gonadal measurement. Parameters measured were: number of gonadal mature broodstocks, gonadosomatic index, fecundity, oocyte diameters, plasma estradiol-17â levels, and gonadal mature levels. The results showed that 0.04 mL Oodev® per g body weight of fish was superior in reproductive performance. In that treatment, there were 76.67% (23/30) fish being matured, gonad somatic index 2.33 ± 1.24%, egg fecundity 73.5 ± 26.2 eggs, oocyte diameters ranged 1.0-1.8 mm, blood level of estradiol-17b 15.9 ± 4.5 rg.mL-1, and the level of gonad development reached to stage IV.
POTENSI “IKAN MURAI AIR TAWAR” (Gymnothorax polyuranodon) SEBAGAI IKAN HIAS Musa, Ahmad; Nur, Bastiar; Hirnawati, Rina
Media Akuakultur Vol 6, No 1 (2011): (Desember 2011)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (564.3 KB) | DOI: 10.15578/ma.6.1.2011.21-24

Abstract

Indonesia merupakan negara yang kaya akan potensi sumberdaya alam hayati termasuk di dalamnya ikan-ikan air tawar, payau, dan laut yang prospeknya dikembangkan sebagai komoditas yang bernilai ekonomi tinggi. Ikan murai air tawar (Gymnothorax polyuranodon) merupakan salah satu spesies ikan yang sebarannya cukup luas di Indonesia. Dalam siklus hidupnya ikan murai ini hidup di air tawar, payau dan laut. Corak warna coklat kekuningan dengan bintik hitam bulat yang tidak teratur pada kepala seperti pita-pita yang warnanya memanjang yang lengkap serta bentuknya yang mirip ular menyebabkan ikan murai ini juga dijadikan ikan hias. Tiga kali koleksi ikan murai di alam telah dilakukan di Sungai Lasusua, Sulawesi Tenggara pada September 2008, Oktober, dan November 2009.
INDUKSI OVULASI DAN PEMIJAHAN IKAN AGAMYSIS (Agamyxis albomaculatus) MENGGUNAKAN HORMON YANG BERBEDA Nur, Bastiar; Permana, Asep; Priyadi, Agus; Mustofa, Siti Zuhriyah; Murniasih, Siti
Jurnal Riset Akuakultur Vol 12, No 2 (2017): (Juni 2017)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (227.142 KB) | DOI: 10.15578/jra.12.2.2017.169-177

Abstract

Ikan agamysis (Agamyxis albomaculatus) merupakan ikan hias air tawar introduksi yang memiliki potensi cukup bagus untuk dikembangkan di Indonesia, namun masih bermasalah dalam budidayanya. Penelitian ini bertujuan untuk menguji efektivitas kombinasi hormon berbeda dalam menginduksi ovulasi dan pemijahan ikan agamysis. Jumlah ikan yang digunakan sebanyak 30 ekor (15 jantan dan 15 betina) dengan ukuran panjang 10-15 cm dan bobot 50-200 g. Ikan uji dipelihara dalam akuarium sistem resirkulasi dan diberi pakan cacing tanah dua kali sehari secara ad libitum. Perlakuan yang diberikan berupa penggunaan beberapa jenis hormon yaitu: (A) LHRHa dan antidopamin (ovaprim) dosis 0,7 mL/kg; (B) human chorionic gonadotropin (hCG) dosis 500 IU/kg; (C) aromatase inhibitor (AI) dosis 10 mg/kg; (D) kombinasi AI dosis 10 mg/kg + ovaprim dosis 0,7 mL/kg; serta (E) kombinasi hCG dosis 500 IU/kg + ovaprim dosis 0,7 mg/kg. Hormon diberikan melalui penyuntikan secara intramuskuler. Hasil penelitian menunjukkan bahwa penggunaan kombinasi hormon hCG dosis 500 IU/kg + ovaprim dosis 0,7 mL/kg; memberikan respons pemijahan terbaik dengan waktu laten 7-12 jam; bobot telur ovulasi 7,3–9,4 g; derajat pembuahan 5,71%-34,7%; dan derajat penetasan 32,5%-50,44%. Penggunaan kombinasi hormon hCG dan ovaprim efektif menstimulasi proses ovulasi telur sehingga dapat menjadi acuan untuk peningkatan produksi larva ikan agamysis dalam pemijahan buatan.Agamysis (Agamyxis albomaculatus) is an introduced freshwater fish that has the potential to be developed in Indonesia. Yet, the fish has not been successfully cultivated. This research was conducted to determine the effectiveness of different hormone combinations to induce ovulation and spawning of agamysis. This research used 30 fishes (15 male and 15 female) ranged 10-15 cm in length and 50-200 g in body weight. The fish were stocked in aquaria equipped with recirculation system and fed with earthworm by ad libitum twice a day. The treatments were: (A) LHRHa and antidopamin (ovaprim) dose of 0.7 mL/ kg, (B) human chorionic gonadotropin (hCG) dose of 500 IU/kg, (C) aromatase inhibitor (AI) dose of 10 mg/kg, (D) a combination of AI dose of 10 mg/kg + ovaprim dose of 0.7 mL/kg, and (E) a combination of hCG dose of 500 IU/kg + ovaprim dose of 0.7 mg/kg. The hormones were given through intramuscularly injection. The results showed that the combination of ovaprim dose of 0.7 mL/kg and hCG dose of 500 IU/kg produced the best spawning response, with the latent period of 7-12 hours, weight of ovulated eggs of 7.3-9.4 g, fertilization rate of 5.71% to 34.7% and hatching rate between 32.5% - 50.44%. In conclusion, the combination of hCG and ovaprim was found to be more effective to stimulate egg ovulation of agamysis and could be used in improving larvae production.
KARAKTER GENOTIPE TIGA POPULASI IKAN RAINBOW AJAMARU (Melanotaenia ajamaruensis) DARI ALAM DAN BUDIDAYA MENGGUNAKAN RAPD Hayuningtyas, Erma Primanita; Sinansari, Shofihar; Fahmi, Melta Rini; Kusrini, Eni; Nur, Bastiar
Jurnal Riset Akuakultur Vol 13, No 2 (2018): (Juni, 2018)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (559.883 KB) | DOI: 10.15578/jra.13.2.2018.105-113

Abstract

Ikan rainbow Ajamaru (Melanotaenia ajamarunensis) yang dinyatakan punah pada tahun 1996 merupakan ikan endemik dari Danau Ajamaru, Papua. Namun ikan ini berhasil ditemukan kembali pada tahun 2007 di Sungai Kaliwensi, Sorong, Papua. Domestikasi ex-situ ikan rainbow Ajamaru sedang dilakukan di Balai Riset Budidaya ikan Hias, Depok-Jawa Barat. Penelitian ini bertujuan mengevaluasi perbedaan genotipe ikan rainbow Ajamaru di alam dan budidaya melalui analisis keragaman genetik untuk melihat adanya perubahan genetik, migrasi maupun mutasi gen. Metode yang digunakan adalah Randomly Amplified Polymorphic DNA (RAPD) dengan 3 jenis primer (OPA 03, OPB 6, dan OPZ 5). Setiap populasi baik, dari alam (Papua) maupun budidaya (Depok dan Papua) masing-masing diambil secara acak sebanyak 10 sampel ikan uji. Hasil penelitian menunjukkan nilai keragaman genetik pada ikan di alam lebih rendah (62,5%) dibanding ikan budidaya di Papua (70,31%) dan tertinggi pada ikan budidaya di Depok (73,43%). Heterozigositas pada ikan di alam lebih rendah (0,172) dibanding ikan budidaya di Papua (0,241) dan di Depok (0,270). Jarak genetik terjauh ditunjukkan antara populasi ikan alam dan populasi ikan budidaya Papua, sedangkan jarak genetik terdekat antara populasi ikan budidaya di Papua dengan di Depok. Karakter genotipe yang dihasilkan pada tiga populasi ikan rainbow Ajamaru adalah memiliki corak DNA yang berbeda nyata (P<0,05). Perbedaan yang dihasilkan dari karakter genotipe karena respon genotip dari tiap individu dan daya adaptasi ikan berbeda-beda pada habitat yang berbeda.Ajamaru rainbow, an endemic fish from Lake Ajamaru, Papua, once declared extinct in 1996. However, it was rediscovered in 2007, in Kaliwensi River, Sorong, Papua. Currently, the Ajamaru rainbow fish is being domesticated ex-situ at the Research Center for Ornamental Fish Culture, Depok, West Java. The aim of the research was to determine the genotype characteristics of wild and cultured Ajamaru rainbow including genetic change, drift, migration, and mutation using genetic variance analysis. The genetic analysis applied was Randomly Amplified Polymorphic DNA (RAPD) using OPA-03, OPB-6, and OPZ-5 primers. Ten samples were used for each population. The results showed that the three populations of Ajamaru rainbow fish have significantly different (P<0.05) of DNA polymorphism. The lowest value of genetic variance was found in the wild fish (62.5%) followed by the cultured fish located in Papua (70.31%), and the highest was observed in the cultured fish located in Depok (73.43%). Heterozygosity of the wild fish was lower (0.172) than that of the cultured fish in Papua (0.241) and in Depok (0.270). The high genetic distance was found between the wild and cultured fish from Papua. The closest relationship was between the fish culture in Papua and Depok. The genotype character produced in the three Ajamaru rainbow fish populations was have significantly different (P<0.05) of DNA polymorphism. The differences that result form genotype characters because of the genotypic response of each individual and the adaptability of fish vary in different habitats. 
KERAGAMAN GENETIK IKAN TIGER FISH (Datnioides sp.) ASAL KALIMANTAN DAN SUMATERA Fahmi, Melta Rini; Hayuningtyas, Erma Primanita; Zamroni, Mochammad; Nur, Bastiar; Sinansari, Shofihar
Jurnal Riset Akuakultur Vol 13, No 3 (2018): (September 2018)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (444.828 KB) | DOI: 10.15578/jra.13.3.2018.191-199

Abstract

Ikan tiger fish (Datnioides sp.) merupakan ikan hias air tawar yang memiliki nilai ekonomis penting. Distribusi populasi ikan ini meliputi Papua, Kalimantan, dan Sumatera, dengan tingkat eksploitasi yang cukup tinggi di dua lokasi terakhir. Penelitian ini dilakukan untuk mendapatkan informasi keragaman genetik ikan tiger fish yang mendiami perairan Kalimantan dan Sumatera. Sebanyak 24 sampel ikan uji dikoleksi dari Sungai Kapuas, Kalimantan Barat dan Sungai Musi, Sumatera Selatan. Penelitian dilakukan dalam dua tahap, tahap pertama yaitu identifikasi molekuler dengan menggunakan DNA barcoding gen cytochrome oxidase 1 (COI), tahap kedua adalah analisis keragaman genetik dengan menggunakan marka DNA mitokondria gen cytochrome b (Cyt b), dan DNA inti gen recombination activating gene (RAG2). Hasil identifikasi secara molekuler menunjukkan bahwa ikan hasil koleksi memiliki kesamaan genetik sebesar 100% dengan spesies D. undecimradiatus. Keragaman genetik ikan tiger fish antar populasi berkisar pada nilai 0,023 (standar deviasi 0,001) sedangkan keragaman intra populasi adalah sebesar 0,002 dan 0,003 masing-masing untuk populasi Kalimantan dan Sumatera. Jarak genetik sampel baik yang berasal dari Sumatera maupun Kalimantan dengan spesies D. undeciumradiatus masing-masing 0,003 dan 0,006; sedangkan dengan spesies D. microlepis yaitu 0,142. Analisis menggunakan gen RAG2 menunjukkan sampel yang diuji memiliki struktur populasi yang terpisah ditandai dengan terjadinya mutasi pada enam nukleotida dan tiga asam amino.The Tiger fish (Datnioides sp.) is a freshwater ornamental fish that has important economic value. The distribution of this fish included Papua, Kalimantan, and Sumatra, but intensive exploitation occurs in the last two population. This research was conducted to obtain the genetic diversity of tiger fish that inhabited in Kalimantan and Sumatra. A total of 24 fish were collected from Kapuas River, West Kalimantan and Musi River, at Sumatra. The study was conducted in two stages, the first stage is molecular identification of sample by using DNA barcoding cytochrome oxidase 1 (COI) gene, the second stage is analyses of genetic diversity of tiger fish within and between population by using the mitochondrial DNA cytochrome b (Cyt b) gene, and nucleus DNA recombination (RAG2) gene. The molecular identification has shown that the collected fish has a genetic similarity of 100% with D. undecimradiatus. The genetic diversity of tiger fish between populations is 0.023 (standard deviation of 0.001) whereas intra-population is 0.002 and 0.003 for Kalimantan and Sumatra, respectively. The genetic distance of samples with species D. undeciumradiatus were 0.003 and 0.006 for Kalimantan and Sumatera, respectively, whereas the genetic distance with D. microlepis was 0.142. The analysis of mutation on RAG2 gene shows there are six nucleotides and three amino acids have mutation.
Optimalisasi reproduksi ikan pelangi kurumoi Melanotaenia parva Allen, 1990 melalui rasio kelamin induk dalam pemijahan [Optimizing of reproduction kurumoi rainbowfish (Melanotaenia parva Allen, 1990 through sex ratio in spawning] Nur, Bastiar; Nurhidayat, nFN
Jurnal Iktiologi Indonesia Vol 12, No 2 (2012): Desember 2012
Publisher : Masyarakat Iktiologi Indonesia (MII )

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (468.899 KB) | DOI: 10.32491/jii.v12i2.116

Abstract

Kurumoi rainbowfish (Melanotaenia parva) is an ornamental fish from “Kurumoi Lake” West Papua, well known for its potential value as an export ornamental fish commodity in Indonesia. The objective of this research was to determine the optimal sex ratios between males and females of kurumoi rainbowfish, in order to obtain its optimum breeding capacity. Broodstocks sex ratio was used as the treatment on this experiment, four treatments were conducted: (A) 1^: 1$, (B) 2^:1$, (C) 3^:1$, and (D) 4^:1$, with four replications for each treatment. The present experiment was conducted in small scale hatchery of Research Centre for Ornamental Fish Aquaculture, Depok, from May to July 2011. Result shown that the treatment B gave the best result (167±30 eggs). This result was significantly different with the treatment D (85±36 eggs), but in contrary not significantly different with the treatment A (132±29 eggs) and C (127±15 eggs). These results were related positively with the fertilization rate for each treatment, which the best fertilization rate were obtained by the treatment B with 99.69±0.63%, however this result was not significantly different with other treatments (P>0.005). The best hatching rate were obtained in treatment D with 96.49±49%, this result was not significantly different with other treatments. The highest survival rate was obtained from treatment B, with 34.21% (not significantly different with other treatments). Based on that result, we can conclude that sex ratio (B) gave the best result on this experiment. AbstrakIkan pelangi kurumoi (Melanotaenia parva) merupakan ikan hias yang berasal dari perairan “Danau Kurumoi” di dae-rah Papua yang berpotensi sebagai komoditas ekspor ikan hias Indonesia. Penelitian ini bertujuan untuk menetapkan rasio kelamin induk jantan dan betina terbaik bagi pemijahan ikan pelangi kurumoi yang optimal. Perlakuan dalam pe-nelitian ini adalah rasio kelamin induk jantan dan betina: (A) 1:1, (B) 2:1, (C) 3:1, dan (D) 4:1; masing-masing perlakuan dipijahkan sebanyak empat kali (ulangan waktu pemijahan). Penelitian ini dilakukan di ruang pembenihan Balai Penelitian dan Pengembangan Budi Daya Ikan Hias (BPPBIH) Depok pada bulan Mei-Juli 2011. Hasil penelitian menunjukkan bahwa pemijahan ikan pelangi pada perlakuan (B) menghasilkan jumlah telur yang dipijahkan (ovulasi) tertinggi yaitu sebanyak 167±30 butir. Hasil ini berbeda secara signifikan dengan perlakuan (D) dengan jumlah telur yang dihasilkan sebanyak 85±36 butir namun tidak berbeda dengan perlakuan (A) dengan jumlah telur yang dihasilkan sebanyak 132±29 butir serta perlakuan (C) dengan jumlah telur yang dihasilkan sebanyak 127±15 butir. Demikian pula halnya dengan derajat pembuahan telur di mana pada perlakuan (B) didapatkan nilai tertinggi yaitu sebesar 99,69±0,63% namun tidak berbeda secara signifikan dengan ketiga perlakuan lainnya (P>0,05). Derajat penetasan tertinggi diperoleh pada perlakuan (D) yaitu sebesar 96,49±49% namun tidak berbeda secara signifikan dengan ketiga perlakuan lainnya. Sintasan larva umur satu bulan tertinggi diperoleh dari hasil pemijahan menggunakan rasio kelamin induk (B) yaitu sebesar 34,21% namun tidak berbeda secara signifikan dengan ketiga perlakuan lainnya. Pemijahan ikan pelangi kurumoi dengan rasio kelamin induk (B) memberikan hasil yang terbaik pada penelitian ini.
PEMIJAHAN DAN PERKEMBANGAN EMBRIO IKAN PELANGI (Melanotaenia spp.) ASAL SUNGAI SAWIAT, PAPUA Nur, Bastiar; Chumaidi, Chumaidi; Sudarto, Sudarto; Pouyaud, Laurent; Slembrouck, Jacques
Jurnal Riset Akuakultur Vol 4, No 2 (2009): (Agustus 2009)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.454 KB) | DOI: 10.15578/jra.4.2.2009.147-156

Abstract

Ikan pelangi asal Sungai Sawiat, Papua merupakan ikan hias endemik yang belum diketahui data biologinya. Penelitian ini bertujuan untuk mengetahui jumlah telur yang dihasilkan, fertilitas dan daya tetas telur serta tahapan perkembangan embrio ikan pelangi asal Sungai Sawiat. Induk jantan dan betina ukuran 10?15 cm sebanyak 20 ekor, dipelihara dalam bak beton berukuran 2,5 x 2,5 x 1,0 m3 dengan sistem resirkulasi dan diberi pakan berupa cacing darah (bloodworm) dengan frekuensi 3 kali sehari secara ad libitum. Dua ekor induk betina dan satu ekor induk jantan yang matang gonad dipijahkan dalam bak beton berukuran 1,0 x 1,0 x 0,75 m3 dan diberi tanaman air berupa eceng gondok sebagai pelindung serta media penempelan telur. Pengamatan ada tidaknya telur dilakukan setiap pagi dan sore hari selama 14 hari. Telur yang didapat dicatat baik fertile maupun infertile. Sebanyak 20 butir telur ditetaskan dalam basket plastik berukuran 13 x 10 x 5 cm dan selanjutnya diamati perkembangan embrionya dengan menggunakan mikroskop. Hasil pengamatan menunjukkan bahwa jumlah telur yang dihasilkan sebanyak 436 butir per dua ekor induk betina (218 butir per ekor), fertilitas 77,06%; daya tetas telur 74,71%; dan telur menetas setelah 8.579 menit (142 jam 59 menit) pada suhu air inkubasi 27,6 ? 28,3oC.Rainbow fish species originated from Sawiat River of Papua is one of Indonesian endemic species which its biological data has not been catalogued properly. The objectives of this research were to know the number of eggs (fecundity), fertility and hatchability and also the stages of embryonic development of the fish. Total of 20 male and female broodstock around 10?15 cm in size, reared in concrete tank sized 2.5 x 2.5 x 1.0 m3 equipped with closed recirculating water system and fed with bloodworm  ad libitum 3 times daily. Two already matured females and one male were selected for natural breeding and then transferred to another concrete tank (1.0 x 1.0 x 0.75 m3 in size) and equipped with water hyacinth as shelter and media for egg attachment. The released eggs were observed twice daily, in the morning and in the afternoon, during the 14 days of research. 20 fertilized eggs were incubated in a plastic basket (13 x 10 x 5 cm in size) for preparation of embryonic development observation under binocular microscope. The result showed that the total egg produced by 2 females were 436 with fertility of 77.06%, and hatchability of 74.71%. Incubated eggs hatched after 8,579 minutes (142 hours and 59 minute) at the temperature range of 27.6?28.3oC.
Pemijahan dan Perkembangan Embrio Ikan Pelangi, Melanotaenia spp. Asal Papua Chumaidi, Chumaidi; Nur, Bastiar; Sudarto, Sudarto; Pouyaud, Laurent; Slembrouck, Jacques
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (34.127 KB) | DOI: 10.22146/jfs.3

Abstract

Rainbow fish (Melanoptaenia spp.) originated from Sungai Gelap of Papua. The objective of this experiment was to know the fecundity, hatching rate and the rate of embryogenesis. Total of 50 male and female broodstock measuring 10?15 cm reared in closed recirculating water system in concrete tank until their gonad matured. One pair composed of one male and two female?s of matured fish was transferred to 1 x 1 x 0.5 m³ concrete tank for pair breeding, the fish was sheltered with water hyacinth. Released eggs were observed in the morning and in the afternoon daily. Eggs were categorized into fertile and infertile. Some 25 eggs were incubated in plastic baskets, and observed under microscope for their embryonic development stages. The results showed that rainbow fish from Sungai Gelap produced eggs (averages) 392 per female; fertility rate was 93.74%; average hatching rate was 87.36%, and the fertilized eggs were hatched approximately 5 days incubation.