Anwar Nur
Jurusan Kimia Fakultas MIPA, Institut Pertanian Bogor, Kampus IPB Baranangsiang, Jl. Pajajaran, Bogor 16144

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Isolasi Identifikasi Bakteri Penghasil Xilanase serta Karakterisasi Enzimnya Richana, Nur; Irawadi, Tun T.; Nur, Anwar; Syamsu, Khaswar
Jurnal AgroBiogen Vol 4, No 1 (2008): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

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Abstract

Xylanase is an extracellular enzyme produced bymicroorganisms. This enzyme is able to hydrolise xylane(hemicellulose) to produce xylooligosaccharide and xylose.Thermoalkaliphilic xylanase is an agent that can be used asa substitute in the pulp whitening process instead of chlorine.A study was done to isolate, identificate of bacteria andcharacterize xylanase. The isolation of xylanase producingbacteria has been done from soil and waste of starch industry.Colonies which produced clearing zone were presumedas xylanolytic bacteria and chosen for further screening.Identification of potential isolate in xylanase production wasdone using 16S ribosomal RNA sequencing. Isolate Bacilluspumilus RXA-III5 originated from lime or alkaline soil wasmore potential isolate in xylanase production than other 24isolates. Precipitation of xylanase, that was done usingammonium sulphate followed by dialyzes produced xylanaseof a higher specific activity (267.1 U.mg-1) than that usingacetone (131.1 U.mg-1) and ethanol (186.65 U.mg-1). Xylanasewas done at purification produced three fractions of xylanase.Xylanase characteristics consist of pH and temperature(9 and 50oC), Km and Vmaks value 6 mg.ml-1 and 0.2mol.minute-1, respectively. The Fe2+ was the strongest activetorand Mg2+ was the strongest inhibitor activity. This enzymewas detected as a cellulose-free xylanase. Xylanase is aprospective agent for bio-bleaching of paper.
Synthesis of Cassava Waste Pulp-Acrylamide Super Absorbent: Effect of Initiator and Cross-Linker Concentration Mas’ud, Zainal Alim; Khotib, Mohammad; Sari, Nurmutia; Nur, Anwar
Indonesian Journal of Chemistry Vol 13, No 1 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2601.851 KB) | DOI: 10.22146/ijc.21328

Abstract

Cassava waste pulp (CWP) contains high carbohydrates that can be modified into super absorbent polymer (SAP) through grafting and cross-linking copolymerization. Acrylamide (AM) was grafted onto CWP with ammonium persulfate (APS) as the initiator and N,N-methylene-bis-acrylamide (MBA) as the cross-linker under atmospheric nitrogen. The effect of APS and MBA concentrations on water absorption capacity of saponified SAP was studied, while the evaluation of grafting ratio (GR) and grafting efficiency (GRE) was conducted on unsaponified SAP. The grafting success was indicated by the occurrence of IR peaks at wave numbers of 573, 765, 858, and 1667 cm-1. In the saponified SAP, the very intense characteristic band at 1562 cm-1 is due to C=O asymmetric stretching in the carboxylate anion. Saponification increases significantly water absorption capacity compared to that of unsaponified SAP (from 39.79 g/g to 578.23 g/g). The highest water absorption capacity is reached at 0.74% APS and 0.09% MBA. The percentage of GRE and GR tends to increase with increasing APS concentration until reaching the highest value and then decreases. Effect of MBA concentration on water absorption capacity, GR, and on GRE is similar to the effect of initiator concentration on GR and GRE.
Seleksi dan Formulasi Media Pertumbuhan Bakteri Penghasil Xilanase Richana, Nur; Irawadi, Tun Tedja; Nur, Anwar; Sailah, Illah; Syamsu, Khaswar
Jurnal Penelitian Pascapanen Pertanian Vol 3, No 1 (2006): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v3n1.2006.41-49

Abstract

Seleksi bakreri penghasil xilanase dan formulasi media pertumbuhan bakteri penghasil xilanase telah dilakukan di laboratorium Bioproses, Balai Besar Penelitian dan Pengembangan Pascapanen Pcrtanian. Seleksi isolat bakteri dilakukan terhadap lima isolat penghasil xilanase yaitu RXAI-5, RXAII-5, RXAIII-1, RXAIII-5 dan RXNI-3), dengan membandingkan hasil kultivasi meliputi biomasa, protein terlarut, akrivitas xilanase dan aktivitas spesifik. Formulasi media dilakukan dengan mengoptimasi konsentrasi peptone, ekstrak khamir sebagai sumbcr nitrogen dan oat spelt xylane sebagai sumber karbon. Analisis percobaan menggunakan rancangan acak faktorial, faktor (A) peptone terdiri atas empat taraf yaitu A1=0, A2=0,I; A3=0,3; A4=0,5% Faktor (B) ekstrak khamir terdiri alas tiga taraf (B1=0,1; B2=0,2; B3=0,3%) dan faktor (C) oat spelt xylene terdiri atas tiga taraf (C1=0,5; C2=0,75; C3=1,0%) dengan tiga kali ulangan. Hasil penelitian menunjukkan bahwa isolat baktcri Bacillus pumilus RXAIII-5 dinyatakan sebagai isolat bakteri unggul diantara kelima isolat bakteri penghasil xilanase. Pada formulasi media ternyata protein terlarut tertinggi (0,596 g/l) pada media dengan komposisi 0,75% xilan, pepton 0,5%, ekstrak khamir 0.2%. Aktiviras xilanase dan aktivitas spesifik tertinggi berturut-turut adalah 186,37 u/ml dan 436,45 U/mg protein. Keduanya dicapai pada komposisi media yang sama yaitu pepton 0, 1 %, ekstrak khamir 0, I %, dan xilan 0,5%. Dcngan demikian komposisi tersebut merupakun komposisi mcdi a terpilih yang optimum. Isolat bakteri unggul bersifat alkali ini diharapkan dapar menghasilkan xilanase yang tahan pada pH tinggi sehingga dapat digunakan untuk proses pemutihan kertas yang ramah  lingkungan. Selection and Growth Medium-Formulation of Xylanase Producing BacteriumThis research was carried out in the bioprocess laboratory of Indonesian Center for Agricultural Postharvest Research and Development, Bogor. Selection of five isolates (RXAI-5, RXAII-5. RXAIII-1, RXAIII-5 and RXNI-3). xylanase producing bacteria Were based on comparative study of cultivation yield consists of biomass of bacterium cells, dissolved protein, xylanase activity and specific activity, Formulation of growth medium using peptone and yeast extract as nitrogen source and oat spclt xylan as carbon source. Design experiment used at formulation of growth medium was randomized factorial design. with factor A) peptone consist of four level, A 1=0. A2=0.1; A3=0.3; A4=0.5%, factor (8) yeast extract consist or third level (131=0.1, 132=0.2; 133=0.3%) and factor (C) oat spelt xylan consist of three level (CI=0.5; C2=0.7S; C3=1.0%), with three replication. Research result showed that Bacillus pumilus RXAIII-5 is the best bacterium isolate among five isolates of xylanase producing bacteria. In growth medium formulation showed that highest dissolved protein (0.596 g/l) was achieved in the medium containing 0.75% xylan, 0.5% pepton, and 0.2% yeast extract. The highest value of both of xylanase activity and specific activity are 186.37 u/ml and 436.45 U/ml respectivelly. In fact these were reached at similar growth medium composition of 0.1 % pepton, 0.1 % yeast extract, and 0.5% xylan, and consequently became the best of growth media formulation. The potential alcaliphilic bacterial isolate is expected to produce xylanase with high pH stability. The enzyme can be used as environmentaly safe agent for paper bleaching.