Widagdo Sri Nugroho
Departemen Kesehatan Masyarakat Veteriner. Fakultas Kedokteran Hewan, Universitas Gadjah Mada

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Detection of Mycobacterium avium subspecies paratuberculosis Formula Milk in Bogor

Jurnal Teknologi Dan Industri Pangan Vol 19, No 1 (2008): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which is usually associated to Crohn’s disease (CD) in human. The disease shows similarities in clinical signs and pathology characteristic with John’s disease (JD) in ruminants which is infected by MAP. Researchers in Europe, USA, and Australia detected MAP in their dairy products and showed the relationship among MAP, CD, and JD. Meanwhile Indonesia imported milk and milk products from those countries to cover the national demand. This situation keeps MAP as potential-problem in national dairy herd and human health in the future. The aim of this study was to detect MAP in the formula milk for toddler. Fifty samples from five established milk producers were taken on August 2006 at the supermarket in Bogor. Two separate diagnostic methods were used parallel in this study i.e polymerase chain reaction method (PCR) with insertion sequence F 57 as the primer and the Mycobacterial Growth Indicator Tube (MGIT). Neither MAP grew in MGIT after 20 weeks of incubation period but 5 samples were found positive by nested PCR. Although there was no evidence weather MAP grew from the samples, as well as in human to provide data on MAP in Indonesia. Key words: Mycobacterium avium subspecies paratuberculosis, growing up milk formula, PCR F57

Detection of Mycobacterium avium subspecies paratuberculosis in Growing up Milk Formula in Bogor

Forum Pasca Sarjana Vol 32, No 2 (2009): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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Abstract

Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which associated with Crohn’s disease (CD) in human and Johne’s disease (JD) in ruminants.  Some researchers in Europe, USA, and Australia detected MAP in the dairy products and showed the relationship among MAP, CD, and JD.  Meanwhile Indonesia imported milk and milk products from those countries to cover national demand.  In the future it will be a potential problem to national dairy herd and human health.  The aim of this study is to detect MAP in the growing up milk formula.  Fifty samples from five established distributors were taken in Bogor.  Some diagnostic methods were used parallel in this study, namely Mycobacterial Growth Indicator Tube (MGIT), Herrold’s Egg Yolk enrichment with mycobactine-J (HEYM) and polymerase chain reaction method (PCR) with insertion sequence IS 900 and F 57 as primer.  Neither MAP grew up in MGIT and HEYM after 20 weeks of incubation period. No positive samples were found by conventional PCR using IS 900 and F57 either but 5 samples were detected positive by nested PCR F57.  Although there was no evidence of MAP grew from the samples in this study, the comprehensive and sustainable studies on MAP still should be carried out with more and varied samples, as well as in human to provide data on MAP and to anticipate it in Indonesia.   Key words: mycobacterium avium subspecies paratuberculosis, growing up milk formula, PCR

PATOGENISITAS ISOLAT Escherichia con POSITIF CONGO RED PADA TELUR AYAM BEREMBRIO UMUR 12 HARI = PATOGENICITY OF CONGO RED POSITIVE ISOLATE OF Escherichia coil IN THE 12-DAYS OLD CHICKEN EMBRYOS

Jurnal Sain Veteriner Vol 20, No 1 (2002)
Publisher : Fakultas Kedokteran Hewan

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Abstract

Penelitian ini bertujuan untuk mengetahui patogenisitas isolat Escherichia coif positif congo red. Escherichia coil dari kasus kolibasilosis ayam diisolasi menggunakan media TSA dan EMB kemudian kemampuan pengikatan warna congo red diuji dengan agar congo red (TSA+0,003% congo red). Tingkat patogenisitas isolat yang mengikat warns congo red dilihat dari uji kematian embrio. Empat isolat E.coli positif conga red (CR+) dan 1 isolat negatif terhadap congo red (CR -) diinokulasikan pada telur berembrio umur 12 hari. Tingkat kematian embrio selama arum bad pascainokulasi masing-masing isolat E.coli positif congo red (500 colony form unit / CM) pada kantung alantois telur berembrio umur 12 hari berbeda antar kelompok. Angka kematian yang diperoleh dari isolat CR + 1, 2, 3, dan 4 masing-masing adalah 10%, 20%, 60% dan 100%. Perubahan anatomi yang tampak yaitu terjadinya perdarahan kulit pada embrio yang mati dan secara mikroskopik lesi-lesi pada hati, jantung, dan limpa menujukkan adanya septisemi. Isolasi dan uji ulang congo red terhadap inokulat positif conga red memperlihatkan bahwa beberapa isolat kehilangan kemampuan mengikat warna conga red. Isolat-isolat tersebut memiliki angka kematian yang rendah (10-20%). Variasi kemampuan isolat mengikat warm conga red memiliki keterkaitan dengan patogenisitasnya.

Kajian Lintas Seksional Infeksi Salmonela pada ayam Ras Petelur Berdasarkan Uji Ulas Kloaka di Kabupaten Sleman Yogyakarta

Jurnal Sain Veteriner Vol 22, No 2 (2004)
Publisher : Fakultas Kedokteran Hewan

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Abstract

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Kemampuan Primer IS900 Dan F57 Mendeteksi Mycobacterium avium Subspesies Paratuberculosis Dengan PCR Konvensional

Jurnal Sain Veteriner Vol 26, No 1 (2008)
Publisher : Fakultas Kedokteran Hewan

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Abstract

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Uji Sensitivitas Bakteri Escherichia Coli Isolat Asal Ayam Yang Bereaksi

Jurnal Sain Veteriner Vol 23, No 1 (2005)
Publisher : Fakultas Kedokteran Hewan

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Abstract

Penelitian ini bertujuan untuk mengetahui resistensi bakteri E.coli isolat ayam yang bereaksi positif pada media congo red terhadap preparat ampisilin, streptomisin, dan enrofloksasin. Delapan biak murni bakteri E.coli yang diisolasi dari kasus kolibasilosis ayam diuji afinitasnya terhadap zat warna merah kongo. Bakteri yang bereaksi positif pada media congo red tersebut, kemudian diuji sensitivitasnya menggunakan media agar Mueller-Hinton, metode disk difusi menurut Kirby-Bouer. Disk antibiotika ampisilin, streptomisin, dan enrofloksasin diposisikan menggunakan dropper pada permukaan kultur bakteri tersebut, kemudian diinkubasi selama 24 jam pada suhu 37°C. Diameter zona terang disekililing disk antibiotik yang terbentuk, kemudian diukur dalam satuan milimeter. Hasil pengukuran tersebut dibandingkan dengan referensi dan atau menurut standar interpretasi Kirby-Bouer. Hasil penelitian menunjukkan bahwa, seluruh bakteri E. coli isolat asal ayam, mempunyai penampilan penotif mengilcat zat warna merah kongo , ternyata 100 % resisten terhadap preparat ampisilin, streptomisin dan enrofloksasin.

ISOLATION AND IDENTIFICATION BACTERIA OF CLINICAL MASTITIS ON ETTAWAH CROSSBRED GOAT

Jurnal Sain Veteriner Vol 31, No 1 (2013)
Publisher : Fakultas Kedokteran Hewan

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Abstract

ABSTACT Ettawah crossbred goat (PE) IS much more usefull to milk goat produce. One of constraints in milk goat is clinical mastitis. Clinical mastitis is economic inflict a financial lost because stopped of milk production, high cost of treatment, and even cause death. The aim of the present study was to determine the kind bacteria that can cause clinical mastitis in PE. A total of 13 clinical mastitis milk and water used to wash the six udder samples were collected from dairy PE farms. All samples were subjected  for isolation and identification of bacteria based on biochemical reactions. From the clinical mastitis milk samples, bacteria isolated were Staphylococcus aureus (55.55%), Pseudomonas sp (27.77%), Streptococcus sp (8.3%) and Bacillus sp (8.33%). Whereas, Pseudomonas sp (77.77%) and Bacillus sp (22.23%) were isolated from the water used to wash the udder. The study showed that more clinical mastitis in PE caused by Staphylococcus aureus. Water use to wash the udder was source of Pseudomonas sp causing clinical mastitis in PE.  

Isolasi and Identification of Bacteria from the Urine Fluid Organic Fertilizer (POC)Ettawa Crossbred (PE) in The Sleman Regency

Jurnal Sain Veteriner Vol 31, No 2 (2013)
Publisher : Fakultas Kedokteran Hewan

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Abstract

Urine is a by product from Ettawa crossbred (PE) goat farm in Sleman regency. In addition, the manure urine goats can be used for fluid organic fertilizer (POC). Recently, organic farming is more interesting for people because it does not use any chemical fertilizers. Salmonella sp and E. coli O157:H7 are pathogenic bacteria that can contaminate agricultural products and dangerous for public health. One of sources of contamination in agricultural products is due to the organic fertilizer. Therefore, the aim of the present study was to determine pathogenic bacteria from POC urine PE goats in Sleman regency. A total of 8 POC samples were collected from PE goat urine in Sleman regency. These samples were analyzed for Salmonella sp, E. coli O157:H7 and other pathogenic bacteria by using biochemical reactions. All samples are negative for Salmonella sp. and E. coli O157:H7 as well. Whereas, 85% of 8 samples are positive for Pseudomonas sp. The present study showed that POC from PE goats farm in Sleman regency are negative for pathogenic bacteria.  

Faktor-Faktor Risiko Mastitis Subklinis pada Kambing Peranakan Etawah di Kabupaten Sleman, Yogyakarta (RISK FACTORS OF SUBCLINICAL MASTITIS ON ETTAWA CROSSBRED GOAT IN SLEMAN REGENCY, YOGYAKARTA)

Jurnal Veteriner Vol 15, No 1 (2014)
Publisher : Jurnal Veteriner

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Abstract

In Sleman, a regency in Yogyakarta special region, Etawah crossbred goats are excessively bred for thedairy produce called the goat’s milk. Subclinical mastitis is one of diseases which reduce the yield of goat’smilk. The aim of this study was to determine the risk factors that contribute to the subclinical mastitis onthe Etawah crossbred goats in Sleman. The 200 samples one of which contains 10 mL of goat’s milk weretaken from the udders of the Etawah crossbred goats from the eight goat farms in Sleman. The 200samples were analyzed for the subclinical mastitis using California Mastitis Test (CMT). The data of riskfactors were gathered through a questionnaire. The risk factors on Etawah crossbred goats in Sleman weredetermine with the use of bivariate analysis chi square (X)2, odds ratio (OR) and relative risk (RR). Thegoat’s milk which subclinical mastitis was isolation and identification of bacteria based on biochemicaltests. The risk factors that cause the subclinicall mastitis on Etawah crossbred goats in Sleman were (1)milk yield (X2=14.23; OR=6.52; RR=4.42), (2) age status of lactation (X2=1.60; OR=59.09; RR=17.94), (3)age of weaning (X2=26.06; OR=2.22; RR=1.91), and (4) Body Condition Score (BCS) (X2=13.89; OR=1.29;RR=1.22). Goat’s milk which subclinicall mastitis were isolated  Bacillus sp  (70%), Staphylococcus sp(33%), Pseudomonas sp (29%), Streptococcus sp (25%), Corynebacterium sp (12%), and E. coli (4%).

Detection of Mycobacterium avium subspecies paratuberculosis of dairy cows in Bogor

Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 4 (2009)
Publisher : Indonesian Animal Sciences Society

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Abstract

Johne’s disease (JD) or partuberculosis is a chronic granulomatous enteritis in ruminants caused by infection of Mycobacterium avium paratuberculosis subspecies (MAP). The disease has been detected serologically in Indonesia. It’s potential to spread to other herds and could create great economic losses. The objectives of current study were to detect MAP in milk and faeces of dairy cows as well as to evaluate the association between farm management factors and presence of the bacteria in dairy cows in Bogor. The sample size was calculated using the formula to detect disease with the prevalence assumed to be 5% using 95% significant level. Milk and faeces samples were taken from 62 dairy cows which were suspected as suffering from MAP infection. Detection of MAP was done by isolation in Herrold’ egg yolk medium with mycobactin J (HEYMj), acid-fast bacilli Ziehl-Neelsen staining, PCR IS900 and F57. Biochemical test to confirm M. tuberculosis presence was also conducted. Fifteen isolates of Mycobacterium sp. were found from the faeces samples but not from the corresponding milk samples. However, conventional PCR conducted on the isolate as well as the milk samples, gave negative results. Biochemical test proved that all Mycobacterium sp. isolates were not M. tuberculosis. This study indicated the prevalence of MAP in Bogor was less than 5%. These findings should be continued by observational study to achieve the comprehensive information at the cattle and herd level. Bovine Tuberculosis monitoring should be done also to protect dairy herd and food safety for the community. Key words: Johne’s disease, Mycobacterium avium subspecies paratuberculosis, Milk, Faeces