Perdana Adhi Nugroho
Cancer Chemoprevention Research Center Fakultas Farmasi, Universitas Gadjah Mada, Yogyakarta.
Articles
2
Documents
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INDUKSI APOPTOSIS EKSTRAK ETANOL CIPLUKAN (Physalis angulata L.) PADA SEL KANKER LEHER RAHIM HeLa MELALUI PENEKANAN EKSPRESI Bel-2

Jurnal Bahan Alam Indonesia Vol 7, No 7 (2011)
Publisher : Indonesian Research Gateway

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Abstract

The incidence of cervical cancer continue to rise encourage a variety of studies to explore the plant as chemopreventive. Ciplukan (Physalis angulata L.) is one of potential plant as chemopreventive agent by regulating cell proliferation, cell cycle and apoptosis. In this study the potential effect of ciplukan ethanolic extract as inducers of apoptosis in human cervical carcinoma HeLa cell line wil be futher investigated. Induction of apoptosis was evaluated by Double Staining methods and Immunocytochemistry. Apoptosis observations qualitatively indicate the occurrence of early apoptosis marked by orange fluorescence and apoptosis bodies because of cell fragmentation. Then the expression of Bcl-2 was observed to know molecular mechanism of apoptosis. Immunocytochemistry experiment showed that Bcl-2 as antiapoptosis protein was inhibited in comparison with control cells. These data indicated that ciplukan ethanolic extract (P.angulata L.) could induced the apoptosis Hela cell line by downregulation of Bcl-2. Study of ciplukan shoud be developed to support ciplukan to be effective anticancer agents.

AKTIVITAS SITOTOKSIK EKSTRAK ETANOLIK HERBA CIPLUKAN (Physalis angulata L.) PADA SEL KANKER LEHER RAHIM HeLa MELALUI MODULASI EKSPRESI PROTEIN p53

Farmasains Vol 1, No 2 (2011): Oktober 2010 - Maret 2011
Publisher : UMM Press

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Abstract

Cervical cancer is one of leading cause of cancer death in women in the developing countries. One of the strategy to prevent cervical cancer based on cytotoxic agents are now being developed. Ciplukan (Physalis angulata L.) is one of potential plant as chemopreventive agent due to Physalin and Withangulatin constituent in this plant. This study was aimed to know cytotoxic effect  of ciplukan ethanolic extract (CEE) in human cervical carcinoma Hela cell line. Evaluation of cell viability value was determined using MTT assay. The expression of p53 as cell proliferation regulator was observed with immunocytochemistry assay. Ethanolic extract of ciplukan showed cytotoxic effect in HeLa cell line with IC50 of 158 µg/ml. Further observation of cell proliferation regulator showed that CEE induces expression of p53 that inhibit cell proliferation. The result showed that CEE has potential activity to be developed as anticancer agent in human cervical cancer.