Articles

Genetic Characterization of Domesticated F1 Generation in Humpback Grouper (Cromileptes altivelis) Aliah, Ratu Siti; Wahidah, .; Sumantadinata, K.; Nugroho, Estu; Carman, O.
Jurnal Akuakultur Indonesia Vol 5, No 1 (2006): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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Abstract

First generation (F1) of hatchery produced humpback grouper (Cromileptes altivelis) has been characterized genetically in order to serve the information of their status in related to their breeding strategy. PCR-RFLP method was used to detect the variation of mtDNA D-loop region of F1 population at BBPBL Lampung and BBAP Situbondo. The result of study showed that reducing of haplotype diversity had been arised from broodstock (0.8548) to F1 generation population (0.7473; 0.7273; and 0.6947, respectively).  Genetic divergence that had found between population BBPBL Lampung and BBAP Situbondo make it possible to do outbreeding in order to get its heterosiss effect. Keywords: mtDNA, haplotype diversity, genetic differentiation, Cromileptes altivelis   ABSTRAK Ikan kerapu tikus (Cromileptes altivelis) generasi pertama (F1) hasil domestikasi di hatchery telah dikarakterisasi secara genetik untuk menyediakan informasi status sehubungan dengan program pemuliaannya.  Metode PCR-RFLP digunakan untuk mendeteksi variasi sekuens D-loop mtDNA ikan kerapu tikus F1 yang diproduksi di BBPBL Lampung dan BBAP Situbondo.  Hasil penelitian menunjukkan bahwa telah terjadi penurunan keragaman haplotipe dari induk (0,8548) ke populasi generasi F1 (masing-masing 0,7473; 0,7273; dan 0,6947).  Adanya keragaman genetik antara populasi ikan kerapu tikus di BBPBL dan BBAP Situbondo memungkinkan dilakukannya outbreeding untuk mendapatkan efek heterosis. Kata kunci: mtDNA, keragaman haplotipe, diferensiasi genetik, Cromileptes altivelis
PENGARUH PENGOLAHAN DAN PENAMBAHAN Na2EDTA PADA TERIGU FORTIFIKASI TERHADAP KETERSEDIAAN BIOLOGIS SENG Nugroho, Estu; Tanziha, Ikeu; Amalia, Leily
Jurnal Gizi dan Pangan Vol 8, No 3 (2013): NOVEMBER 2013
Publisher : Jurnal Gizi dan Pangan

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Abstract

ABSTRACTThe objective of this research was to analyze changes in bioavailability of zinc (Zn) caused by addition of Na2EDTA and processing of fortified wheat flour. Bioavailability of zinc was tested by in vitro method as a simulation of human digestive track. The result showed that there was no significant difference in the bioavailability of zinc on wheat flour which is processed by frying (donuts), steaming (steamed bun), and baking (bread) (p>0.05). However, there was a tendency to increase the bioavailability of wheat flour processed by frying (donuts) and baking (bread) when compared to the raw dough. Raw dough had bioavailability of zinc 13.29%, after frying (donut) increase to 14.72%, and after baking (bread) increase to 17.06%. The addition of Na2EDTA also did not increase bioavailability of zinc significantly for the three types of processing (p>0.05), despite increased bioavailability of zinc on the donuts by 4.67%.Keywords: bioavailability, in vitro, Na2EDTA, wheat flour, zincABSTRAKTujuan penelitian ini adalah menganalisis perubahan ketersediaan biologis seng (Zn) yang terjadi akibat penambahan Na2EDTA dan pengolahan terigu yang difortifikasi. Uji ketersediaan biologis seng dilakukan secara in vitro dengan mensimulasikan pencernaan manusia. Hasil uji menunjukkan bahwa tidak terdapat perbedaan ketersediaan biologis seng yang nyata pada terigu yang digoreng (donat), dikukus (bakpau), ataupun dipanggang (roti) (p>0.05). Namun, terdapat kecenderungan peningkatan ketersediaan biologis pada terigu yang diolah dengan cara digoreng (donat) dan dipanggang (roti) dibandingkan dengan adonan mentah. Adonan mentah memiliki nilai ketersediaan biologis seng sebesar 13.29%, setelah digoreng (donat) memiliki nilai ketersediaan biologis seng sebesar 14.72% dan setelah dipanggang (roti) memiliki nilai ketersediaan biologis seng sebesar 17.06%. Penambahan Na2EDTA juga tidak memberikan peningkatan ketersediaan biologis seng yang nyata pada ketiga jenis pengolahan yang digunakan (p>0.05) walaupun terjadi peningkatan ketersediaan biologis seng pada donat sebesar 4.67%.Kata kunci: in vitro, ketersediaan biologis, Na2EDTA, seng, terigu
Genetic Characterization of Domesticated F1 Generation in Humpback Grouper (Cromileptes altivelis) Aliah, Ratu Siti; Wahidah, .; Sumantadinata, K.; Nugroho, Estu; Carman, O.
Jurnal Akuakultur Indonesia Vol 5, No 1 (2006): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.5.87-96

Abstract

First generation (F1) of hatchery produced humpback grouper (Cromileptes altivelis) has been characterized genetically in order to serve the information of their status in related to their breeding strategy. PCR-RFLP method was used to detect the variation of mtDNA D-loop region of F1 population at BBPBL Lampung and BBAP Situbondo. The result of study showed that reducing of haplotype diversity had been arised from broodstock (0.8548) to F1 generation population (0.7473; 0.7273; and 0.6947, respectively).  Genetic divergence that had found between population BBPBL Lampung and BBAP Situbondo make it possible to do outbreeding in order to get its heterosis's effect. Keywords: mtDNA, haplotype diversity, genetic differentiation, Cromileptes altivelis   ABSTRAK Ikan kerapu tikus (Cromileptes altivelis) generasi pertama (F1) hasil domestikasi di hatchery telah dikarakterisasi secara genetik untuk menyediakan informasi status sehubungan dengan program pemuliaannya.  Metode PCR-RFLP digunakan untuk mendeteksi variasi sekuens D-loop mtDNA ikan kerapu tikus F1 yang diproduksi di BBPBL Lampung dan BBAP Situbondo.  Hasil penelitian menunjukkan bahwa telah terjadi penurunan keragaman haplotipe dari induk (0,8548) ke populasi generasi F1 (masing-masing 0,7473; 0,7273; dan 0,6947).  Adanya keragaman genetik antara populasi ikan kerapu tikus di BBPBL dan BBAP Situbondo memungkinkan dilakukannya outbreeding untuk mendapatkan efek heterosis. Kata kunci: mtDNA, keragaman haplotipe, diferensiasi genetik, Cromileptes altivelis
ANALISIS KERAGAMAN GENETIK BEBERAPA POPULASI IKAN BATAK (Tor soro) DENGAN METODE RANDOM AMPLIFIED POLYMORPHISM DNA (RAPD) Nugroho, Estu; Soewardi, Kadarwan; Kurniawirawan, Adi
Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Vol 14, No 1 (2007): Juni 2007
Publisher : Institut Pertanian Bogor

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Abstract

Penelitian ini bertujuan untuk mengetahui keragaman genetik ikan batak dari beberapa lokasi yang berbeda. Ikan batak yang digunakan dalam penelitian ini dikumpulkan dari Sungai Tarutung, Sungai Bahorok, Sungai Aek Sirambe dan Sungai Asahan di provinsi Sumatera Utara, dan dari Kabupaten Sumedang, Jawa Barat. Penelitian ini menggunakan metode Random Amplified Polymorphism DNA (RAPD). Hasil penelitianmenunjukkan bahwa nilai rata-rata heterozygote adalah antara 0.0909 ? 0.1407. Populasi ikan batak dariTarutung dan Bahorok memiliki jarak genetik paling dekat dengan nilai 0.1272 dan populasi ikan batak dari Asahan dan Aek Sirambe memiliki jarak genetik paling jauh dengan nilai 0.3106. Dari dendogram jarak genetik terlihat bahwa terjadi dua pengelompokkan populasi, kelompok pertama terdiri dari populasi Tarutung, Bahorok dan Asahan, dan kelompok kedua terdiri dari populasi Sumedang dan Aek Sirambe.Kata kunci: ikan batak, Tor soro, RAPD. 
PENGARUH PENGOLAHAN DAN PENAMBAHAN Na2EDTA PADA TERIGU FORTIFIKASI TERHADAP KETERSEDIAAN BIOLOGIS SENG Nugroho, Estu; Tanziha, Ikeu; Amalia, Leily
Jurnal Gizi dan Pangan Vol 8, No 3 (2013)
Publisher : Food and Nutrition Society of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.154 KB) | DOI: 10.25182/jgp.2013.8.3.221-228

Abstract

The objective of this research was to analyze changes in bioavailability of zinc (Zn) caused by addition of Na2EDTA and processing of fortified wheat flour. Bioavailability of zinc was tested by in vitro method as a simulation of human digestive track. The result showed that there was no significant difference in the bioavailability of zinc on wheat flour which is processed by frying (donuts), steaming (steamed bun), and baking (bread) (p>0.05). However, there was a tendency to increase the bioavailability of wheat flour processed by frying (donuts) and baking (bread) when compared to the raw dough. Raw dough had bioavailability of zinc 13.29%, after frying (donut) increase to 14.72%, and after baking (bread) increase to 17.06%. The addition of Na2EDTA also did not increase bioavailability of zinc significantly for the three types of processing (p>0.05), despite increased bioavailability of zinc on the donuts by 4.67%.
KARAKTERISASI GENETIK IKAN LELE DUMBO BERDASARKAN MARKER RAPD FINGERPRINTING [Genetic Characterization of African Catfish Revealed by RAPD Fingerprinting Markers] Nugroho, Estu; Putera, Sabara
BERITA BIOLOGI Vol 17, No 1 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4060.656 KB) | DOI: 10.14203/beritabiologi.v17i1.2869

Abstract

African catfish, namely lele dumbo, is an economically important fresh water fish in Nusa Tenggara Barat. There are three strains of African catfish that are well distributed among farmers. Genetic information of three strain catfish stocks is needed in order to sustain their development program. Genetic variations of the three strains of catfish, i.e. Sangkuriang, Masamo, Paiton has been evaluated using RAPD markers. DNA genome was extracted using phenol-chloroform methods. DNA was amplified using 20 primers (OPA 1 – 20). The results showed that three of the 20 primers have good amplification products. There was no significant genetic differences among three strains of catfish analyzed (P>0.05). Genetic variation of three African catfish stocks ranges from low to middle value. The highest genetic variation is found in strain Masamo with heterozygosity of 0.273 (with 70% polymorphism loci), followed by Sangkuriang 0.189 (60%) and Paiton0.147 (40%). Hybrid candidates of Masamo-Sangkuriang have eterozygosity ranges between 0.256 - 0.306 (with 66.6 7 – 73.77% polymorphism loci). Strain Masamo and Paiton has more close genetic relationship than among the both and Sangkuriang.
HERITABILITAS DAN PEROLEHAN GENETIK PADA BOBOT IKAN NILA HASIL SELEKSI [Heritability and Genetic gain on Weight of Tilapia Resulted Frown Individual Selection] Nugroho, Estu; Mayadi, Lalu; Budileksono, Sigit
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3300.024 KB) | DOI: 10.14203/beritabiologi.v16i2.2689

Abstract

Directional-individual selection on tilapia growth had been conducted in West Nusa Tenggara Province. The base population had been constructed through di allele crossing between six tilapia strains i.e. NIRWANA, BEST, SULTANA, Citralada, JATIMBULAN and White Sleman. A total of 150 brood stock pairs were used for spawning with the ratio of female and male parent 1: 1 in net with size of 1x1x1 m. Two hundred larvae  produced from each spawn pairs were communally reared in a pond for three months. Directional-individual selection was conducted at the size of > 50 grams. The cut-off for minimum selected fish was based on the lowest individual weight of 10% top population. The average value of heritability on body weight was 0.251 for males and 0.258 for the female population. The total value of genetic gain of body weight for four generations was 51.68% for male population and 56.78% for the female population.
ANALISIS KERAGAMAN GENETIK BEBERAPA POPULASI IKAN BATAK (Tor soro) DENGAN METODE RANDOM AMPLIFIED POLYMORPHISM DNA (RAPD) Nugroho, Estu; Soewardi, Kadarwan; Kurniawirawan, Adi
Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Vol 14, No 2 (2007): Desember 2007
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Penelitian ini bertujuan untuk mengetahui keragaman genetik ikan batak dari beberapa lokasi yang berbeda. ikan batak yang digunakan dalam penelitian ini di kumpulkan dari sungai tarautung , sungai Baho-rok. sungai Aek Sirambe dan sungai asahan dinprovinsi sumatera utara , dan dari kabupaten sumedang,jawa barat . penelitian ini menggunakan metode random Amplified Polymorphism DNA (RPAD). Hasil penelitian menunjukan bahwa nilai rata-rata heterrozzygote adalah antara 0.0909-0.1407. populasi ikan batak dari tarutung bahorok memiliki jarak genetik paling dekat dengan nilai 0.1272 dan populasi ikan batak dari asahan dan aek sirambe memiliki jarak genetik paling jauh dengan nilai 0.3106 dari dendogram jarak genetik terlihat bahwa terjadi dua pengelompokan populasi , kelompok pertama terdiri dari populasi tarutung, bahorok dan asahan, dan kelompok kedua terdiri dari populasi sumedang dan Aek Sirambe Kata Kunci: ikan batak, Tor soro ,RAPD
EVALUASI VARIASI GENETIK RAS-RAS IKAN GURAME DENGAN MENGGUNAKAN MARKER DNA Nugroho, Estu
Jurnal Perikanan Universitas Gadjah Mada Vol 13, No 2 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.3066

Abstract

Penelitian ini dilakukan untuk mengamati variasi genetik strain Bastar, Paris dan Blusafir dengan menggunakan penanda RAPD. DNA ikan gurame diekstraksi dari sirip, dan diamplifikasi secara random dengan menggunakan primer OPA 1-20. Tidak terdapat perbedaan secara genetik antara tiga strain yang diuji. Berdasarkan dua primer RAPD (OPA 4 dan 7), variabilitas tertinggi diamati pada Bluesafir dengan nilai heterogenitas 0,3050 diikuti dengan Paris dengan nilai 0,2832 dan Bastar dengan nilai 0,2360. Jarak rata Nei genetik adalah 0,118, dengan terendah yang teramati antara Paris dan Bluesafir.
KERAGAMAN GENETIK POPULASI IKAN NILA {Oreochromis niloticus) DALAM PROGRAM SELEKSI BERDASARKAN RAPD Arifin, Otong Zenal; Nugroho, Estu; Gustiano, Rudhy
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (442.087 KB) | DOI: 10.14203/beritabiologi.v8i6.826

Abstract

Objectives of the study was to discover genetic variability and genetic relationship of paternal half sib population of nile tilapia (Oreochromis niloticus) under Selection Program Scheme at Research Institute for Freshwater Aquaculture, in Bogor, West Java.Four populations from unrelated selected breeders were observed for genetic variability using RAPD. The analysis of amplification from each locus and fragments were used to estimate DNA polymorphisms, heterozygosity, fst and genetic distance.The range of heterozygosity of four examined populations was 0.1760-0.2168 with level polymorphic between 47.66% and 64.86%.The highest heterozygosity and polymorphic was on population 1 and the lowest one was on population 2. Fst test showed significance among the populations. The closest relationship was between population 1 and 4 (0.1978) and the furthest was population 2 and 3 (0.3289).
Co-Authors . Wahidah Achmad Zamroni, Achmad Adi Kurniawirawan, Adi Ali, Fauzan Alimuddin Alimuddin Anwar Bey Pane Arifin, Otong Zaenal Arifin, Otong Zaenal Asih, Sidi Asih, Sidi Ath-thar, M. Hunaina Fariduddin Azrita, Azrita Bajar, Mochamad Budi Nugraha, Budi Budi Setyono Budileksono, Sigit Budileksono, Sigit Dahelmi Dahelmi DEDI SOEDHARMA Didik Ariyanto Gleni Hasan Huwoyon, Gleni Hasan Gunawan Gunawan Hafrijal Syandri Hamid, Mimid Abdul Hermawan, Hery Sulistio Ikeu Tanziha Imron Imron, Imron Irin Iriana Kusmini, Irin Iriana Jatnika, Jatnika Jefry Jack Mamangkey Jojo Subagja K. Sumantadinata Kadarwan Soewardi Komar Sumantadinata Kristanto, Anang Hari Kristanto, Anang Hari Kristanto, Anang Hari Leily Amalia Mayadi, Lalu Mayadi, Lalu Megawati, Novi Mulyasari, Mulyasari Mulyasari, Mulyasari Mulyasari, Mulyasari Mulyono Sumitro Baskoro, Mulyono Sumitro Ningrum Suhenda O. Carman ODANG CARMAN Otong Zenal Arifin Panggabean, Maria Goretti Lily Prakoso, Vitas Atmadi Prihadi, Andung Santoso Priono, Bambang Priyanto, Dwijo Priyanto, Dwijo Priyatna, Fatriyandi Nur Putera, Sabara Putra, Sabara Rachman, Nunung Nur Raden Roro Sri Pudji Sinarni Dewi Rahayuni, Evi Ratu Siti Aliah Refilza, Refilza Reza Samsudin Rudhy Gustiano Rustadi Rustadi Saepudin Saepudin Setijaningsih, Lies Sjafei, Djadja Subardja Sri Sundari Subagyo Subagyo SUBANDRIYO SUBANDRIYO Sudarto Sudarto Sueb, Mochammad Sugiyono Sugiyono Sukadi, Mochammad Fatuchri Sulhi, Muhammad Sulistiono Sulistiono Sulistyo, Hery Sunaryo Sunaryo Susila, Susila Sutrisno Sukimin Suwarso Suwarso Syahdan, Mohammad Ali Syaifullah Syaifullah Syandri, Hafrizal Titin Kurniasih Tri Heru Prihadi Tutik Kadarini Wartono Hadie, Wartono Wasito, Bagus Widiyati, Ani Widiyati, Ani Zafril Imran Azwar, Zafril Imran Zulkifli Zulkifli