Nugrahaningsih Nugrahaningsih
Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Semarang

Published : 13 Documents
Articles

Found 13 Documents
Search

PROFIL KEKUATAN TARIK GELATIN DARI KULIT KAKI AYAM DAN KECEPATANPENUTUPAN LUKA PADA Mus musculus PASCA PEMBERIAN GELATIN

Prosiding Seminar Biologi Vol 11, No 1 (2014): Seminar Nasional XI Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

Show Abstract | Original Source | Check in Google Scholar | Full PDF (416.278 KB)

Abstract

Gelatiniskind of protein extracted from animal collagen tissue. Chicken legs skin waste has not been used optimally, where as in the chicken legs skin contains collagent hatcan be extracted into gelatin. This study aims to: (1) measuring the tensile strength of gelatin produced fromthe washing process with different volumes of water flow, (2) determine the speed of enchance wound healing process in Mus musculus. Result were analyzedby ANOVA. The analysis showed differences in the volume of water flowingin the washing processafter the soakedin H2SO4did not give an affect to the tensile strength. Gelatin(P5) presence best tospeed upthe process of skin enchance wound to Musmusculus. Keywords: tensile strength of gelatin, enhances wound healing process

PROFIL KEKUATAN TARIK GELATIN DARI KULIT KAKI AYAM DAN KECEPATANPENUTUPAN LUKA PADA Mus musculus PASCA PEMBERIAN GELATIN

Prosiding Seminar Biologi Vol 11, No 1 (2014): Seminar Nasional XI Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

Show Abstract | Original Source | Check in Google Scholar | Full PDF (416.278 KB)

Abstract

Gelatiniskind of protein extracted from animal collagen tissue. Chicken legs skin waste has not been used optimally, where as in the chicken legs skin contains collagent hatcan be extracted into gelatin. This study aims to: (1) measuring the tensile strength of gelatin produced fromthe washing process with different volumes of water flow, (2) determine the speed of enchance wound healing process in Mus musculus. Result were analyzedby ANOVA. The analysis showed differences in the volume of water flowingin the washing processafter the soakedin H2SO4did not give an affect to the tensile strength. Gelatin(P5) presence best tospeed upthe process of skin enchance wound to Musmusculus. Keywords: tensile strength of gelatin, enhances wound healing process

Developing of instructional media-based animation video on enzyme and metabolism material in senior high school

Jurnal Pendidikan Biologi Indonesia Vol 3, No 3 (2017): NOVEMBER
Publisher : University of Muhammadiyah Malang

Show Abstract | Original Source | Check in Google Scholar | Full PDF (478.343 KB)

Abstract

The research aimed to product a learning material related to animation video on enzyme and metabolism material for high school student which is validated by media and material experts, educational practition and student legibility. Research and development model is ADDIE with quantitative-qualitative data analyzing methode. Data collection was obtained from validation results by media and material experts, educational partition and student legibility. The validation results were scores and suggestion. The percentage of product from expert media validation (100%), expert material validation (89,58%), educational practition (84,61%), and student legibility (81,91%) showed valid of the criteria and feasible to use after revision.

IDENTIFIKASI APOPTOSIS DENGAN METODE TUNEL PASCA PEMBERIAN EKSTRAK SAMBILOTO DAN PENGARUHNYA TERHADAP VOLUME TUMOR

Sainteknol : Jurnal Sains dan Teknologi Vol 12, No 2 (2014): December 2014
Publisher : Unnes Journal

Show Abstract | Original Source | Check in Google Scholar

Abstract

Apoptosis adalah kematian sel terprogram (programed cell death) yang bertujuan untuk mempertahankan kestabilan populai sel. Kegagalan pengaturan apoptosis dapat menyebabkan sel membelah tanpa terkendali, yang disebut sebagai kanker. Peningkatan apoptosis merupakan suatu upaya yang dikembangkan sebagai terapi kanker. Pemeriksaan apoptosis dengan metode TUNEL(Terminal deoxyribonucleotidyl transferase dUTP nick end labeling) dapat memberikan gambaran proses apoptosis pada tingkat sel tunggal sehingga lebih spesifik dan memiliki akurasi tinggi. Sambiloto merupakan salah satu tanaman obat tradisional yang digunakan sebagai anti kanker. Beberapa penelitian yang dilakukan secara in vitro menunjukkan pengaruh andrografolid yang diisolasi dari sambiloto terhadap apoptosis sel kanker pada kultur. Hasil tersebut masih memerlukan penelitian lebih lanjut, terutama pengaruhnya bila diberikan secara oral. Penelitian ini dilakukan untuk membuktikan pengaruh ekstrak sambiloto terhadap apoptosis dan volume tumor bila diberikan secara oral dan diidentifikasi dengan metode TUNEL. Penelitian dilakukan dengan desain Randomized post test control group. Sebanyak 24 ekor mencit C3H yang telah tumbuh kanker mamma dibagi secara acak menjadi 4 kelompok masing-masing 6 ekor. Diberikan ekstrak sambiloto dengan dosis 5, 10 dan 15 mg per ekor per hari, dan kelompok kontrol. Pemberian ekstrak sambiloto dilakukan secara oral selama 14 hari. Pengukuran volume tumor dilakukan pada hari ke 1, 4, 8, 12 dan 15. Pada hari ke 15 mencit dimatikan dengan menggunakan eter. Jaringan kanker dibuat blok parafin untuk pemeriksaan apoptosis dengan metode TUNEL. Hasil Penelitian menunjukkan adanya peningkatan signifikan sel apoptosis (p=0,000), dengan dosis terbaik 15 mg/hari. Ada beda yang bermakna pada rerata kelipatan penambahan volume tumor (p=0,049). Hasil uji post hoc menunjukkan bahwa perbedaan terletak antara kelompok kontrol dengan kelompok dosis 10 mg/hr. Dapat disimpulkan bahwa pemberian ekstrak sambiloto secara oral meningkatkan sel yang mengalami apoptosis dengan pemeriksaan TUNEL namun belum dapat menurunkan volume tumor.

Andrographis paniculata extract induced apoptosis of adenocarcinoma mammae in C3H mice

Universa Medicina Vol 32, No 2 (2013)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Original Source | Check in Google Scholar

Abstract

BACKGROUNDApoptosis plays an important role in tumorigenesis. Induction of apoptosis is a strategy for developing cancer therapy. In vitro study found that andrographolide isolated from Andrographis paniculata has anticancer activity by an apoptotic mechanism in cancer cell lines. The aim of the present study was to prove theeffect of Andrographis paniculata extract administered orally on apoptosis of mammary adenocarcinoma in C3H mice.METHODSThis study was of post test randomized control group design. Twenty four C3H mice with transplanted mammary adenocarcinomas were divided into four groups. To three groups Andrographis paniculata extract was administered orally for14 days, at doses of 5, 10 and 15 mg/day, respectively, whereas to the control group no Andrographis paniculata extract was administered. On day 15 the mice were terminated. The mammary adenocarcinomas were examined by the terminal deoxynucleotide transferase dUTP nick end labeling (TUNEL) method. The values of the apoptotic index were expressed as mean±SD and analyzed using ANOVA and Pearson’s correlation test.RESULTSThe mean apoptotic index values differed significantly among the experimental groups (p=0.001). The highest value was found in the group receiving Andrographis paniculata extract 15 mg/day, while the lowest was in the control group, the values being significantly correlated (r=0.974).CONCLUSIONSOral administration of Andrographis paniculata extract induced apoptosis in C3H mice with mammary adenocarcinoma

Study of Anthocyanin Pigment in Three Different Sepal Colour of Hortensia (Hydrangea macrophylla)

Proceeding International Conference on Global Resource Conservation Vol 4, No 1: Proceeding of 4th ICGRC 2013
Publisher : Proceeding International Conference on Global Resource Conservation

Show Abstract | Original Source | Check in Google Scholar

Abstract

Anthocyanin is a natural pigment that express orange, red, blue, and purple color. It can be accumulated in leaves, thorn, tuber, fruit, flower, and seed. Hortensia (Hydrangea macrophylla) which is well-known as ‘Panca Warna’, is a kind of plant that easy to thrive in different climates, can express different sepal colour as the effect of environmental factor such as soil pH. The aim of this study is to determine the level of anthocyanin pigment in three different sepal colour of Hortensia. This research is a descriptive quantitative which used randomized block design with nine replications. The object of this research is three different sepal colour of Hydrangea which obtained from Flower Gardens in Sidomulyo florist. Data obtained through the differential pH spectroscopy method and was analyzed using one way ANOVA. The result reveals that there is no significant differences of anthocyanin level among the samples.Keywords: anthocyanin pigment, sepal colour, Hortensia (Hydrangea macrophylla)

Study of Anthocyanin Pigment in Three Different Sepal Colour of Hortensia (Hydrangea macrophylla)

Proceeding International Conference on Global Resource Conservation Vol 4, No 1: Proceeding of 4th ICGRC 2013
Publisher : Proceeding International Conference on Global Resource Conservation

Show Abstract | Original Source | Check in Google Scholar

Abstract

Anthocyanin is a natural pigment that express orange, red, blue, and purple color. It can be accumulated in leaves, thorn, tuber, fruit, flower, and seed. Hortensia (Hydrangea macrophylla) which is well-known as ‘Panca Warna’, is a kind of plant that easy to thrive in different climates, can express different sepal colour as the effect of environmental factor such as soil pH. The aim of this study is to determine the level of anthocyanin pigment in three different sepal colour of Hortensia. This research is a descriptive quantitative which used randomized block design with nine replications. The object of this research is three different sepal colour of Hydrangea which obtained from Flower Gardens in Sidomulyo florist. Data obtained through the differential pH spectroscopy method and was analyzed using one way ANOVA. The result reveals that there is no significant differences of anthocyanin level among the samples.Keywords: anthocyanin pigment, sepal colour, Hortensia (Hydrangea macrophylla)

Developing an Organic Liquid Fertilizer Booklet to Support The Sustainable Reserve Food Garden (SRFG) Program at Tunggak Cerme, Wonomerto, Probolinggo

Indonesian Journal of Biology Education Vol 1, No 1 (2018): INDONESIAN JOURNAL OF BIOLOGY EDUCATION
Publisher : Universitas Tidar

Show Abstract | Original Source | Check in Google Scholar

Abstract

The aim of this study is to develop and test organic liquid fertilizer booklet to support SRFG program. This study used ADDIE model which consists of analyze, design, develop, implement, and evaluation. The product tests are validity test and practicality test. The validity test result by the material expert, media expert, and field practitioner are 95,5%, 98,5%, 97,9%, showed that the booklet is valid. The practicality test result is 91,1%, showed that the booklet is practical.

Ekspresi VEGF Sel Adenokarsinoma Mamma Pada Pemberian Oral Ekstrak Andrographis paniculata

JURNAL ILMU KEFARMASIAN INDONESIA Vol 13 No 1 (2015): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

Show Abstract | Original Source | Check in Google Scholar

Abstract

its metastase.  Vascular endothelial growth factor (VEGF)  was a potential  proangiogenic factor of angiogenesis process.  In  vitro  study  of Andrographis paniculata extract  showed  decreasing tend of VEGF level serum on PC-3 cancer cell line. The aim of this study was to prove the effect of oral administered Andrographis paniculata extract on VEGF expression of adenocarcinoma mammae C3H mice. Twenty four mice was transplanted with adenocarcinoma mammae randomly to four groups. Andrographis paniculata extract at dose 5, 10, and 15 mg/day were given on mice group along 14 days. One group another as a control. VEGF expression were examined use immunohistochemistry staining. Anova test showed difference VEGF expression among reseach groups (p=0,000, r=-0,953). The highest of VEGF expression index 24,8 was found on control group, and the lowest 19,4 was found on group which received 15 mg/day. We concluded that oral administration of  Andrographis paniculata extract decreased VEGF expression of adenocarcinoma mamme C3H mice.

Analysis of Implementation Plan of Science Learning and the Factors of ITS Implementation in MTs and MA Al Khoiriyyah Semarang

Journal of Innovative Science Education Vol 7 No 2 (2018)
Publisher : Postgraduate, Universitas Negeri Semarang, Indonesia

Show Abstract | Original Source | Check in Google Scholar | Full PDF (278.714 KB)

Abstract

The learning process requires a plan, so that what is done can run and produce something as expected. With such a plan, the process to be carried out over a long period of time has a clear direction, predictable results, predictable resources required, and can be used to determine the requirements of students in following the learning process in the school. The purpose of this study is to analyze the conformity and implementation of RPP, as well as supporting factors and inhibiting the implementation of science learning in MTs and MA Al Khoiriyyah Semarang. The method used in this research is qualitative research with case study method. This research takes place in Madrasah Tsanawiyah (MTs) and Madrasah Aliyah Al Khoiriyyah Semarang. Sources of data in this study are teachers of science lessons in MTs Al Khoiriyyah and physics, chemistry, biology teacher at MA Al Khoiriyyah. Data collection techniques used consisted of observation, interview, documentation and student response questionnaire. The results showed that the quality of RPP in MTs and MA Alkhoiriyyah was in accordance with Permendikbud number 22 of 2016 with very good criteria. The implementation of science learning in MTs and MA Alkhoiriyyah has been in accordance with Permendikbud number 22 of 2016 with good category. Factors supporting the implementation of RPP in learning is the source of learning and supporting facilities such as libraries, laptops, LCDs, and the internet. While the inhibiting factors are time constraints, students are less active, and the teacher's understanding is still lacking in combining the methods, models, and learning strategies with a scientific approach. Implementation of science learning activities in MTs and MA Al Khoiriyyah received a good response from learners.