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PENGARUH JENIS INOKULUM TERHADAP PRODUKSI ASAM STI´RAT Nugrahani, Arsa Wahyu; Rinihapsari, Elisa
STIFAR - Sekolah Tinggi Ilmu Farmasi Vol 3, No 2 (2008)
Publisher : STIFAR - Sekolah Tinggi Ilmu Farmasi

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Abstract

Aspergillus niger is the highest citric acid producer. It will be better if other species like yeast can also produce citric acid faster and greater. Mold and yeast were inoculated in medium containing glucose as carbon source. Medium was shaked 7 x 24 hours where every 24 hours some medium sample was taken to measure citric acid concentration using volumetric method with neutral reaction. The data was analysed using two way anova. The result showed that yeast produced citric acid but not more than mold. In 7 x 24 hours citric acid concentration increased. It caused by fermentation maximum. It was concluded that citric acid production was influenced by the different inoculums species.   Keywords : inoculum, fermentation, citric acid
UJI ANTI DIARE INFUSA DAUN MIMBA (Azadirachta indica Juss) TERHADAP MENCIT JANTAN GALUR SWISS Puspitaningrum, Ika; Nugrahani, Arsa Wahyu; Suwarmi, STIFAR
STIFAR - Sekolah Tinggi Ilmu Farmasi Vol 8, No 2 (2013)
Publisher : STIFAR - Sekolah Tinggi Ilmu Farmasi

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Abstract

This study aims to determine the efficacy of neem leaf infusion antidiarrheal (Azadirachta indica Juss.) against male mice strain Swiss, and to determine the effective dose infusion of neem as antidiarrheal. Object of this study was the time of the occurrence, duration of diarrhea, and stool consistency and number of male mice strains Swiss. Samples of fresh neem leaves is used in the preparations made ​​by the infusion technique is random sampling . A 25 of mice was divided into 5 groups consisting of groups I- III were given neem leaf infusion dose of 68.12 mg/kgbw; 204.36 mg/kgbw and 613.08 mg/kgbw, group IV as a positive control group that were given loperamide HCl dose of 0.73 mg/kgbw, and group V as a negative control group that were given 0.75 ml of oleum ricini. All administration is done orally. Observations include the time of occurrence of diarrhea , duration of the course of diarrhea, stool consistency and number of each were evaluated statistically by Anova method and post- ANOVA test . The results showed that Infuse leaves of neem has antidiarrheal efficacy of male mice strain Swiss. Neem leaf infusion at a dose of 204.36 mg/kgbw and 613.08 mg/kgbw have antidiarrheal effects comparable to loperamide HCl dose of 0.73 mg / kgbw.   Keyword: infusa, Mimba leaves, diarhea
Screening of Antibacterial and Anticancer Activity of Soft Corals from Togean Islands, Indonesia Zubair, Muhammad Sulaiman; Lallo, Subehan; Rusmiyanti, Rusmiyanti; Nugrahani, Arsa Wahyu; Jantan, Ibrahim
Indonesian Journal of Pharmacy Vol 29 No 4, 2018
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1388.045 KB) | DOI: 10.14499/indonesianjpharm29iss4pp173

Abstract

Soft corals (Octocorallia, Alcyonaceae) have been reported to possess diverse biological activities and unique structural chemistry. This study aims to screen the potential antibacterial and anticancer activity of some soft corals collected from Togean Islands, Central Sulawesi, Indonesia. They were Lobophytum sp, Sarcophyton sp, Sinularia sp 1, and Sinularia sp 2. All dried coral materials were extracted for 3 x 24 h by maceration method using methanol and then evaporated by rotary evaporator to obtain viscous extracts. The determination of antibacterial activity had been performed by well agar diffusion method against Staphylococcus aureus and Escherichia coli. Meanwhile, the cytotoxic activity was performed by MTT method, followed by apoptosis annexin V-FTIC assay agains. Identification for the presence of terpenoids was performed by vacuum p-anisaldehyde-sulphuric acid spraying reagent on thin layer chromatography (TLC). Sinularia sp2 extract have strongly inhibited S. aureus and E.coli with the diameter of inhibition range from 12.76mm and 17.86mm, respectively. Moreover, Sinularia sp2 extract possessed also cytotoxic activity against human breast adenocarcinoma (MCF-7) and colorectal carcinoma (HCT-116) with the IC50 of 46.807 and 47.186 μg/mL, respectively.  Extract Sinularia sp 1 was found to have strongest cytotoxicity on human colon colorectal carcinoma (HCT-116) with the IC50 of < 1.505 μg/mL. Annexin V-FTIC assay clearly exhibited that the apoptosis mechanism is proposed by the extracts of Sinularia sp1 and Sinularia sp 2. Terpenoids were identified on both  extracts suggesting for further purification and isolation for the bioactive terpenoid compounds. 
Uji Aktivitas Antibakteri Ekstrak Serbuk Gergaji Kayu Eboni (Diospyros celebicaBakh.) Terhadap Bakteri Staphylococcus aureus dan Escherichia coli Wahyuni, Wahyuni; Ibrahim, Nurlina; Nugrahani, Arsa Wahyu
Biocelebes Vol 12, No 1 (2018)
Publisher : Biocelebes

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Abstract

Ebony (Diospyros celebica Bakh.) is one of the beneficial plants from ebenaceae, especially in traditional medicine. The sawdust extract containing chemical compounds such as tannins, saponins and terpenoids. This research were aimed to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) also to identified the active compounds from sawdust extracts of D. celebica Bakh. against Staphylococcus aureus and Escherichia coli. The Research methods include extraction using maceration method by ethanol 96%. MIC and MBC determination using dilution and diffusion methods. The Results showed that MIC value of S.aureus and E. coli were 6% and 7%, respectively. While MBC value of S. aureus and E. coli were 12% and 13%, respectively. Identification of chemical compounds were determined by Thin Layer Chromatography (TLC) bioautography method by 10% H2SO4 and anisaldehid-sulfuric acid reagent spray. There were two chemichal compounds that produced, saponin and terpenoid.
STUDI TANAMAN BERKHASIAT OBAT SUKU MORI DI KECAMATAN PETASIA, PETASIA BARAT, DAN PETASIA TIMUR KABUPATEN MOROWALI UTARA SULAWESI TENGAH Idris, Idris; Ibrahim, Nurlina; Nugrahani, Arsa Wahyu
Biocelebes Vol 12, No 1 (2018)
Publisher : Biocelebes

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Abstract

This study aims to conduct an inventory, knowing how to use and organs of plants used as medicine by Mori Tribal in Petasia, Petasia and East Petasia District, West North Morowali Regency of Central Sulawesi. This study used qualitative methods and sampling techniques that snowball sampling, with open-ended interview techniques interview on media 9 informants obtained using a questionnaire. Based on the results of research known as 79 species of medicinal plants, 2 of which can not be identified and divided into 45 familia used as a medicine. Plants are most widely used is of familia Euphorbiaceae as many as 8 species. Organs of plants used include leaves, stems, fruits, rhizomes, seeds, bark, flowers, bulbs, herbaceous roots and sap. Organ plant is widely used is the leaves that percentage utilization is 58%. Mori Tribal communities in the District, West Petasia and East Petasia North Morowali District use medicinal herbs to treat the disease, healing and health maintenance. The cooking methods include boiling water, brewed, boxed, taped, squeezed, scrubbed/smeared, chewed and consumed directly, drops, and sprayed. How to use include drunk, eaten, chewed and consumed directly, polished/smeared, attached, dropped, and chewed and sprayed on the affected area.
Aktivitas Antibakteri Ekstrak Etanol Daun Jarak Merah (Jatropha gossypifolia) Terhadap Bakteri Escherichia coli dan Staphylococcus aureus Torokano, Semuel; Khumaidi, Akhmad; Nugrahani, Arsa Wahyu
Natural Science: Journal of Science and Technology Vol 7, No 1 (2018): Volume 7 Number 1 (March 2018)
Publisher : UNIVERSITAS TADULAKO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/25411969.2018.v7.i1.9925

Abstract

dengan Jarak Pagar (Jatropha curcas) yang sebelumnya telah terbukti memiliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri, konsentrasi optimum antibakteri dan senyawa yang berperan terhadap aktivitas antibakteri dari ekstrak etanol daun jarak merah (Jatropha gossypifolia L.) terhadap Staphylococcus aureus dan Escherichia coli. Ekstraksi senyawa aktif menggunakan metode maserasi dengan pelarut etanol. Pengujian aktivitas antibakteri dilakukan dengan varian konsentrasi 20%, 40%, 60%, 80% dan 100% dengan metode difusi agar. Hasil penelitian menunjukkan bahwa ekstrak etanol daun jarak merah memiliki aktivitas antibakteri terhadap            S.aureus dan E.coli dengan konsentrasi optimum penghambat pertumbuhan bakteri yaitu 60%         (S.aureus) dan 80% (E.coli). Berdasarkan pengujian bioautografi dan KLT dengan eluen                   n-heksana:etil asetat (1:2) menggunakan pereaksi kromogenik senyawa yang diduga berperan terhadap aktivitas antibakteri adalah terpenoid
ETNOBOTANI SUKU TOGIAN DI PULAU MALENGE KECAMATAN TALATAKO, KABUPATEN TOJO UNA-UNA, SULAWESI TENGAH Tabeo, Dalton Fidel; Ibrahim, Nurlina; Nugrahani, Arsa Wahyu
Biocelebes Vol 13, No 1 (2019)
Publisher : Biocelebes

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/j25805991.2019.v13.i1.12868

Abstract

Ethnobotany study in Togian Tribe has done in Tojo Una-Una, Central Sulawesi. This study was aimed to find out the information about the types of plants, used parts, processing, usage and dosage as well as knowing what type of disease treated using medicinal plants by Togian tribal communities in Malenge Island, Talatako District, Tojo Una- una, Central Sulawesi. This research  was a descriptive study with qualitative methods and used snowball sampling through interviews on four informants with the questionnaire. The results showed that 60 species are divided into 33 families. the most widely used was the Asteraceae by 7 species and Fabaceae familia by 6 species. Plant parts include leaves, stems, fruit, roots, rhizomes, herbs, seeds, sap and flowers partnered with the largest percentage of leaves 56%. the using and processing of medicinal plants by the Togian Tribe Malenge Island were boiled, mashed, squeezed, shredded, soaked, drunk, chewed, dropped, scrubbed, and pasted. the most  using and processing were boiled and drunk.
STUDI ETNOBOTANI TUMBUHAN BERKHASIAT OBAT PADA SUKU TAU TAA WANA DI DESA BULAN JAYA KECAMATAN AMPANA TETE, KABUPATEN TOJO UNA UNA, PROVINSI SULAWESI TENGAH Hijrah, Hijrah; Nugrahani, Arsa Wahyu; Ramadanil, Ramadanil
Biocelebes Vol 13, No 1 (2019)
Publisher : Biocelebes

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/j25805991.2019.v13.i1.12876

Abstract

Ethnobotanical study of Tau Taa Wana Tribe aims to determine the types and parts of plants used as traditional medicine as well as knowing how to use medicinal plants by Tau Taa Wana Tribe in the Bulan Jaya Village Ampana Tete District, Tojo Una Una Regency, Central Sulawesi Province. The study was a descriptive research using qualitative methods with the technique of sampling snowball sampling. Information obtained through open-ended interviews at 9 informants interviewed based on the questions that had been developed previously. The results showed that there were 69 species of plants that are divided into 38 families used as medicine. The most widely used plants originate from Asteraceae and Solanaceae family each 5 species. Part used include leaves, stems, fruits, roots, rhizomes, tubers, herbs, seeds, bark and flowers. Percentage of part medicinal plants most widely used are the leaves (32%). The most used method is to be drunk.
Uji Aktivitas Antibakteri Formula Pasta Gigi Ekstrak Batang Karui (Harrisonia Perforata Merr.) Terhadap Bakteri Streptococcus Mutans Nurjannah, Wardatul; Yusriadi, Yusriadi; Nugrahani, Arsa Wahyu
Biocelebes Vol 12, No 2 (2018)
Publisher : Biology Department, Mathematics and natural science

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Abstract

Karui (Harrisonia perforata Merr.) is one of the many species that live in tropical regions such as Central Sulawesi (Palu) and has antibacterial activity. This research aims to find the variation effect of karui stem extract (Harrisonia perforata Merr.) in toothpaste towards stability of physical quality and antibacterial activity bacteria Streptococcus mutans. The concentrations of karui stem extract 1%, 2%, and 3% in toothpaste tested for antibacterial activity using pitting method which further tested for statistical analysis "One Way Anova" using SPSS 16.0 software. The results of the evaluation of toothpaste Karui stem extract F1 (1%), F2 (2%), and F3 (3%) showed that the test of the organoleptic and homogenity has no significant difference during the storage time (28 days) but the test at pH, viscosity, and the scatter has significant difference. The Formula of karui stem ekstract with 3 concentrations F1 (1%), F2 (2%), and F3 (3%) forming an average diameter of antibacterial inhibitory 6.65 mm; 7.14 mm; and 9.85 mm. The results of statistical analyzed from toothpaste test diameter inhibitory zone showed an unsignificant difference. Keywords: toothpaste, extract, karui (Harrisonia perforata Merr.), Streptococcus mutans
Aktivitas Antibakteri Fraksi Allium ascalonicum Linn A562275sal Lembah Palu terhadap Shigella dysenteriae Khumaidi, Akhmad; Maulina, Kumalahayati; Nugrahani, Arsa Wahyu
JURNAL ILMU KEFARMASIAN INDONESIA Vol 17 No 2 (2019): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1375.333 KB) | DOI: 10.35814/jifi.v17i2.728

Abstract

Bawang merah (Allium ascalonicum Linn.) dari lembah Palu merupakan salah satu tanaman khas Sulawesi Tengah yang digunakan sebagai obat tradisional seperti untuk mengobati penyakit infeksi. Penelitian ini bertujuan untuk mengetahui fraksi yang mempunyai aktivitas tinggi dari sampel umbi dan daun bawang merah dari lembah Palu dalam menghambat pertumbuhan bakteri Shigella dysenteriae dan menentukan golongan senyawa yang memiliki aktivitas tersebut. Penelitian dengan ekstraksi bawang merah menggunakan  metode maserasi dengan etanol 96 % terhadap kedua sampel. Fraksinasi cair-cair digunakan sebagai metode fraksinasi menggunakan pelarut heksan, etil asetat dan air secara berurutan. Uji aktivitas antibakteri menggunakan metode difusi dengan teknik sumuran dan KLT Bioautografi. Hasil penelitian menunjukkan bahwa fraksi etil asetat memberikan aktivitas tertinggi dari sampel umbi, sedangkan pada sampel daun fraksi etil asetat dan fraksi heksana mampu memberikan aktivititas penghambatan yang baik. Golongan senyawa yang teridentifikasi dengan metode kromatografi lapis tipis adalah senyawa flavonoid untuk sampel umbi (Rf 0,18). Pada sampel daun teridentifikasi senyawa steroid pada fraksi etil asetat (Rf 0,62) dan senyawa terpenoid (Rf 0,48) pada fraksi heksana. Berdasarkan hal tersebut Bawang merah dari  lembah Palu berpotensi dapat dikembangkan sebagai bahan antibakteri.