Jusak Nugraha
Clinical Pathology Department, Dr. Soetomo Hospital Surabaya Institute of Tropical Disease, Universitas Airlangga Surabaya

Published : 37 Documents
Articles

Found 37 Documents
Search

Diagnostic Value of Anti-Mutated Citrullinate Vimentin and Rheumatoid Factor With Immunochromatographic Method in Early Rheumatoid Arthritis Patients Nugraha, Jusak; Rahmawati, Febtarini; Soeroso, Joewono
Journal of the Indonesian Medical Association Vol. 62 No. 5 May 2012
Publisher : Journal of the Indonesian Medical Association

Show Abstract | Original Source | Check in Google Scholar

Abstract

Introduction: The ACR/EULAR (American College of Rheumatology and European LeagueAgainst Rheumatism) criteria for rheumatoid artritis (RA) 2010 includes two laboratory parameters, which are rheumatoid factor (RF) and anti-citrullinated protein antibodies (ACPA), such as anti-MCV (anti-mutated citrullinated vimentin) or anti-CCP (anti-cyclic citrullinated protein antibody), besides ESR or CRP. Diagnostic specificity of RF is poor, especially in elderly. Most specific autoantibody system for RA is the family of autoantibodies directed to citrullinecontaining proteins. The biomarker of anti-MCV is a further development after the anti-CCP; and anti-MCV shows a superior sensitivity compared to anti-CCP2 and RF for early RA patients. The aim of study: Was to confirm the efficacy of a new practical, accurate, and efficient test for diagnosys of rheumatoid arthritis.Methods: From the patients attending rheumatology outpatient clinics in Dr. Soetomo HospitalSurabaya during April to September 2011, 33 new RA patients fulfilling ACR/EULAR criteriaand 32 other arthritis patients with the same age characteristic were obtained.Results: The new and combined anti-MCV and RF-ICT test revealed sensitivity of 100%, specificity of 68,75%, positive predictive value of 76,74% and negative predictive value of 100%. When each parameter was compared to standard Elisa method, they showed a good conformity and no significance difference was shown.Conclusion: This new test is suitable for screening purpose because it has a high sensitivity andalso have a high negative predictive value to rule out the possibility of RA. J Indon Med Assoc. 2012;62:189-93.Keywords: rheumatoid arthritis - ACR/EULAR 2010 - anti-MCV
ASSOCIATION BETWEEN ATYPICAL DEPOLARIZATION IN CELLDYN 00 AND THE PRESENCE OF PLASMODIUM SPP IN BLOOD IN Dr. SOETOMO HOSPITAL SURABAYA Nugraha, Jusak
Indonesian Journal of Tropical and Infectious Disease Vol 2, No 1 (2011)
Publisher : Institute of Topical Disease

Show Abstract | Original Source | Check in Google Scholar

Abstract

Background: Malaria is a parasitic disease worldwide with a high morbidity and mortality. A rapid and accurate method is needed to detect the presence of malaria parasites in blood. A flagging system atypical depolarization (atypdep) in CBC results from Cell-Dyn 3200 has been related with malaria infection. Materials and Methods: An observational cross sectional approach with 48 samples obtained from inpatients of the Dr.Soetomo Hospital, Surabaya. Samples were screened by Cell-Dyn 3200 analyzer for atypdep flagging in CBC. Positive samples were later confirmed by microscope to detect malaria parasites. results: From 48 samples with atypdep flagging, 7 samples were malaria positive on peripheral blood smear (13.1%). Most frequent atypdep flagging was seen in malignancy (18.7%), and approximately 54.6% of the samples were not accompanied by fever symptoms. Lekositosis and anemia each were found in 20 samples (41.6%) and thrombocytopenia in 33.3%. conclusion: The presence of atypdep flagging in Cell-Dyn 3200 does not necessarily indicate the existence of malaria or it could be said that atypdep flagging is not always associated with presence of malaria infection. The usage of an atypdep flagging in non-endemic areas such as Surabaya is just an alert sign to evaluate malaria infection rather than a screening method to detect malaria.
PLATELET RICH PLASMA PREPARATION PROTOCOLS: A PRELIMINARY STUDY Nugraha, Hans Kristian; Muljanti, Meiti; Hernaningsih, Yetti; Nugraha, Jusak
Indonesian Journal of Tropical and Infectious Disease Vol 3, No 2 (2012)
Publisher : Institute of Topical Disease

Show Abstract | Original Source | Check in Google Scholar | Full PDF (101.43 KB)

Abstract

Currently, therapy with Platelet Rich Plasma (PRP) has been widely used and continues to grow for various clinical applications. Along with its development, there are various options in the method of obtaining PRP, either automatic or manual, while one of the most reliable methods according to the literature is a double centrifugation method. The purpose of this research is to produce anoptimization of the double centrifugation method. This study used experimental data obtained by conducting a research at the Clinical Pathology Laboratory of Dr. Soetomo Hospital, Surabaya. Experiments were conducted on stored blood obtained from the blood bag from Indonesian Red Crossand fresh blood from healthy donors with CPD anticoagulant. Results: PRP with optimum platelet count could be made with sufficient personal laboratory skills and amounted to 4.11 times with the platelet count of 1.152 million using 1300 rcf for 5 minutes for the first centrifugation, and 2300 rcf for 7 minutes for the second centrifugation.
Diagnostic Value of Anti-Mutated Citrullinate Vimentin and Rheumatoid Factor With Immunochromatographic Method in Early Rheumatoid Arthritis Patients Nugraha, Jusak; Rahmawati, Febtarini; Soeroso, Joewono
JOURNAL OF THE INDONESIAN MEDICAL ASSOCIATION Majalah Kedokteran Indonesia Vol 62, No 5 (2012): Journal of the Indonesian Medical Association Majalah Kedokteran Indonesia
Publisher : PENGURUS BESAR IKATAN DOKTER INDONESIA

Show Abstract | Original Source | Check in Google Scholar | Full PDF (1419.592 KB)

Abstract

Background: The ACR/EULAR (American College of Rheumatology and European League Againts Rheumatism) criteria for rheumatoid artritis (RA) 2010 includes two laboratory parameters, which are rheumatoid factor (RF) and anti-citrullinated protein antibodies (ACPA), such as anti-MCV (anti-mutated citrullinated vimentin) or anti-CCP (anti-cyclic citrullinated protein antibody), besides ESR or CRP.
ASSOCIATION BETWEEN ATYPICAL DEPOLARIZATION IN CELLDYN 00 AND THE PRESENCE OF PLASMODIUM SPP IN BLOOD IN Dr. SOETOMO HOSPITAL SURABAYA Nugraha, Jusak
Indonesian Journal of Tropical and Infectious Disease Vol 2, No 1 (2011)
Publisher : Institute of Topical Disease

Show Abstract | Original Source | Check in Google Scholar | Full PDF (1434.143 KB)

Abstract

Background: Malaria is a parasitic disease worldwide with a high morbidity and mortality. A rapid and accurate method is needed to detect the presence of malaria parasites in blood. A flagging system atypical depolarization (atypdep) in CBC results from Cell-Dyn 3200 has been related with malaria infection. Materials and Methods: An observational cross sectional approach with 48 samples obtained from inpatients of the Dr.Soetomo Hospital, Surabaya. Samples were screened by Cell-Dyn 3200 analyzer for atypdep flagging in CBC. Positive samples were later confirmed by microscope to detect malaria parasites. results: From 48 samples with atypdep flagging, 7 samples were malaria positive on peripheral blood smear (13.1%). Most frequent atypdep flagging was seen in malignancy (18.7%), and approximately 54.6% of the samples were not accompanied by fever symptoms. Lekositosis and anemia each were found in 20 samples (41.6%) and thrombocytopenia in 33.3%. conclusion: The presence of atypdep flagging in Cell-Dyn 3200 does not necessarily indicate the existence of malaria or it could be said that atypdep flagging is not always associated with presence of malaria infection. The usage of an atypdep flagging in non-endemic areas such as Surabaya is just an alert sign to evaluate malaria infection rather than a screening method to detect malaria.
Nilai Diagnostik Rapid Test TbAg DAN MPT64 dengan Kultur Sebagai Gold Standard Nazarudin, Muhammad; Nugraha, Jusak; -, Aryati
Biosains Vol 17, No 3 (2015)
Publisher : Universitas Airlangga

Show Abstract | Original Source | Check in Google Scholar | Full PDF (14.778 KB)

Abstract

AbstrakTuberkulosis (Tb) sampai kini masih dianggap sebagai salah satu penyakit berbahaya yang ada di muka bumi. Pada tahun 2012, diperkirakan 8,6 juta orang mengidap Tb dan 1,3 juta di antaranya meninggal dunia. Mycobacterium tuberculosis sebagai penyebab penyakit Tb, dapat dideteksi dengan pengecatan BTA untuk mewarnai bagian sitoplasma dari bakteri ini. Standar terbaik dalam pemeriksaan Tb adalah kultur bakteri, namun dinilai terlalu lama dalam memberikan hasil. Beberapa produsen mengembangkan alat yang mempermudah prosedur pemeriksaan, memberikan hasil yang cepat namun tetap berkualitas, diantaranya yang beredar di Indonesia adalah TbAg Rapid Test dan MPT64 Rapid Test. Metode penelitian ini adalah analitik observasional dengan rancangan cross sectional. Dilakukan terhadap 50 responden dengan 34 responden mengidap Tb paru. Data yang diuji nilai diagnostik, uji McNemar dan uji Kappa. Nilai sensitivitas, TbAg Rapid Test dengan sampel sputum 97.5% dan isolat 50%. Nilai sensitivitas MPT64 Rapid Test dengan sampel sputum 11.76% dan isolat 95%. Hasil BTA dan TbAg Rapid Test juga tidak menunjukkan perbedaan berarti sehingga dapat digunakan untuk pemeriksaan skrining dan MPT65 Rapid Test dapat gunakan untuk tes biokimia setelah kultur. Kata Kunci : Tuberkulosis, TbAg Rapid Test, MPT64 Rapid Test
Analisis Kadar IFN-γ dan IL-10 pada PBMC Penderita Tuberkulosis Aktif, Laten dan Orang Sehat, Setelah di Stimulasi dengan Antigen ESAT-6 Setiawan, Heru; Nugraha, Jusak
Jurnal Biosains Pascasarjana Vol 18, No 1 (2016)
Publisher : Universitas Airlangga

Show Abstract | Original Source | Check in Google Scholar | Full PDF (906.06 KB)

Abstract

Abstrak Latar Belakang : Tuberkulosis paru disebabkan oleh bakteri Mycobacterium tuberculosis, yakni baksil tahan asam yang hidup secara intraseluler dan merupakan penyebab utama kematian penyakit menular. Oleh karenanya, perlu diagnosis dini untuk pencegahan. Sel Th1 sangat berperan pada sistem pertahanan tubuh dalam menghadapi infeksi bakteri intraseluler. Pada tuberkulosis paru, terjadi ketidakseimbangan sistem imun host. Tujuan penelitian ini adalah menganalisis kadar IFN-γ dan IL-10 pada PBMC (peripheral blood mononuclear cell) penderita tuberkulosis aktif, laten dan orang sehat, setelah stimulasi dengan antigen ESAT-6. Metode Penelitian : Subjek penelitian adalah 10 TB aktif, 10 LTBI dan 10 orang sehat, yang diambil dari RS Paru Karang Tembok Surabaya. Dilakukan pengambilan darah vena kemudian diisolasi PBMCnya dan dikultur dengan ESAT-6. Kemudian supernatan diambil untuk dilakukan pemeriksaan kadar IFN-γ dan IL-10 dengan metode pemeriksaan Enzyme Linked Immunosorbent Assay (ELISA).Hasil : Kadar IFN-γ setelah di stimulasi antigen ESAT-6, pada orang sehat berkisar antara 95,84 – 135,47 pg/mL, pada TB laten 74,6 – 142,2 pg/mL dan TB aktif 72,67 – 154,21 pg/mL. Kadar  IL-10 setelah di stimulasi antigen ESAT-6, pada orang sehat berkisar antara 161,89 – 282,57 pg/mL, pada TB laten 145,28 – 218,64 pg/mL dan TB akti 232,05 – 294,07 pg/mL. Hasil analisis menunjukkan terdapat perbedaan bermakna (p=0,000) kadar IL-10 pada ketiga kelompok.Simpulan : Terdapat perbedaan pola sekresi/ kadar IFN-γ dan IL-10 pada PBMC penderita TB aktif, TB laten dan orang sehat. Kata kunci : IFN-γ, IL-10 , TB aktif.
PLATELET RICH PLASMA PREPARATION PROTOCOLS: A PRELIMINARY STUDY Nugraha, Hans Kristian; Muljanti, Meiti; Hernaningsih, Yetti; Nugraha, Jusak
Indonesian Journal of Tropical and Infectious Disease Vol 3, No 2 (2012)
Publisher : Institute of Topical Disease

Show Abstract | Original Source | Check in Google Scholar | Full PDF (101.43 KB)

Abstract

Currently, therapy with Platelet Rich Plasma (PRP) has been widely used and continues to grow for various clinical applications. Along with its development, there are various options in the method of obtaining PRP, either automatic or manual, while one of the most reliable methods according to the literature is a double centrifugation method. The purpose of this research is to produce anoptimization of the double centrifugation method. This study used experimental data obtained by conducting a research at the Clinical Pathology Laboratory of Dr. Soetomo Hospital, Surabaya. Experiments were conducted on stored blood obtained from the blood bag from Indonesian Red Crossand fresh blood from healthy donors with CPD anticoagulant. Results: PRP with optimum platelet count could be made with sufficient personal laboratory skills and amounted to 4.11 times with the platelet count of 1.152 million using 1300 rcf for 5 minutes for the first centrifugation, and 2300 rcf for 7 minutes for the second centrifugation.
THE DIFFERENCE OF MAP1LC3 LEVEL AS MACROPHAGE AUTOPHAGY MARKER BETWEEN RESISTANT AND SENSITIVE TUBERCULOSIS PATIENTS ON RIFAMPICIN Wulandari, Dian Novita; Nugraha, Jusak; Soedarsono, Soedarsono
Indonesian Journal of Tropical and Infectious Disease Vol 7, No 1 (2018)
Publisher : Institute of Topical Disease

Show Abstract | Original Source | Check in Google Scholar | Full PDF (426.587 KB)

Abstract

M. tuberculosis (MTB) is an intracelular bacteria that live in the host macrophage cells. Several organs can be affected by tuberculosis but most major illnesses are lung diseases. Immediately after infection, MTB will be phagocytosed by the alveolar macrophage cells and can survive in the phagosome. The macrophage plays a role in innate immunity towards an infection using autophagy by removing the microbe directly via phagocytosis. When bacteria phagocytosized, vacuole membrane formed double membranes called autophagosome, and followed by degradation by lysosome, which known as  autolysosome. Induction of autophagy can be observed on the formation of microtubule-associated proteins 1B lightchain 3B (MAP1LC3B/LC3). MAP1LC3B is protein that have role at autophagic way for selection autophagy substrate and biogenesis. In this study we are used serum from patients TB with rifampicin resistant and rifampicin sensitive as control. Samples were divided using gene expert to differentiate between resistant and sensitive rifampicin.This research aims to compare MAP1LC3B levels in resistant and sensitive rifampicin to study macrophages respond in autophagic way in tuberculosis patients, and give information for define therapy plan to improve therapy for MDR-TB patients. Type of this research is a case control study design with cross sectional research with each groups sample is 19 from age 18-65 years old. Result, MAP1LC3B serum levels on the rifampicin resistant group are lower compared to rifampicin sensitive group. This occur because MTB is able to hide and evade innate immune defense mechanisms. MTB can maintain intracellular growth inside the phagosome by inhibiting phagolysosome formation in autophagy process especially inhibit MAP1LC3B formation by PDIM. 
CORRELATION BETWEEN THE USE OF ALBUMIN INFUSION AND CONCENTRATIONS OF SERUM ALBUMIN, PROINFLAMMATORY CYTOKINES (TNF-, IL6) AND SOFA SCORE IN SEPTIC PATIENTS Utariani, Arie; Prasetyo, Budi; Nugraha, Jusak
Folia Medica Indonesiana Vol 52, No 4 (2016): OCTOBER - DECEMBER 2016
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Original Source | Check in Google Scholar | Full PDF (75.452 KB)

Abstract

Sepsis is a major problem causing high morbidity and mortality in patients who hospitalized in intensive care. In sepsis, there is a change in the distribution of albumin in the intravascular and extravascular compartment, so hipoalbumin is occured. Using albumin in septic patients from several studies that have been done are still controversial. Analyzing albumin infusion with changes in levels of albumin, cytokines and SOFA score in septic patients in intensive care dr. Soetomo. The design of this study is a prospective longitudinal observational. The study was conducted in the intensive care unit or ICU dr. Soetomo in February 2015. The inclusion and exclusion criteria were determined that obtained 15 research subjects. They were examined with serum albumin and proinflammatory cytokines as well as evaluating the SOFA score. Then, it measure with ANOVA test methods. Infusion of albumin positively correlated with increased levels of serum albumin and a negative correlation with the levels of IL6, TNF-a and SOFA score. It also found a correlation between changes in levels of albumin with changing levels of IL6 (p <0:01) and changes in SOFA score (p <0.05).