Savitri Novelina
Bagian Anatomi Histologi dan Embriologi, Departemen Anatomi Fisiologi dan Embriologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor

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SEL PENGHASIL LISOZIM TERDETEKSI PADA KELENJAR LUDAH SAPI DENGAN TEKNIK IMUNOHISTOKIMIA LYZOZYME PRODUCING CELLS ARE DETECTED IN THE BOVINE SALIVARY GLAND BY IMMUNOHISTOCHEMICAL TECHNIQUE

Jurnal Veteriner Vol 8, No 1 (2007)
Publisher : Jurnal Veteriner

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Abstract

Lisozim bersifat bakteriolotik dengan cara merusak ikatan glikosida pada dinding bakteri sehingga pelacakan sel penghasil lisozim pada kelenjar ludah sapi perlu dilakukan. Dalam penelitian ini, sel penghasil lisozim diperiksa menggunakan teknik pewarnaan histologi dan imunohistokimia. Hasil penelitian menunjukkan bahwa kelenjar parotis sapi bersifat serous murni. Kelenjar submandibularis bagian asinarnya terdiri atas sel-sel serous dan sel-sel mukous. Sel-sel asinar mukous terlihat lebih dominan jika dibandingkan dengan sel-sel asinar serous. Sel-sel asinar kelenjar parotis sapi bereaksi positif dengan intensitas sedang (++) terhadap pewarnaan imunohistokimia. Sel-sel epitel duktus kelenjar bereaksi sedang (++) sampai dengan kuat (+++). Pola sebaran lisozim yang terdeteksi pada penelitian ini menunjukkan kemiripan dengan hewan mamalia pada umumnya. Sel-sel asinar serous pada kelenjar submandibularis memberikan reaksi positif dengan intensitas rendah (+) sampai dengan sedang (++) dan sel-sel epitel duktus kelenjar dengan intensitas kuat (+++). Lisozim ditemukan pada bagian sel-sel asinar serous dan sel-sel epitel duktus kelenjar parotis dan submandibularis.

Comparative Microanatomy of The Local Goat and Sheep Pancreas Islets With a Special Reference to The Distribution and Relative Frequency of Glucagon Producing Cells

Media Veteriner Vol 8, No 1 (2001): Media Veteriner
Publisher : Media Veteriner

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Abstract

Penelitian ini memanfaatkan teknik pewarnaan standardan khusus, impregnasi perak Grimelius, untukmenggambarkan morfologi komparasi pankreas kambing dandomba lokal dengan tinjauan khusus pada distribusi danfrekuensi sel-sel penghasil hormon glukagon pada bagianendokrin pankreas. Pankreas domba mempunyai lobulasiyang lebih jelas daripada pankreas kambing ditandai dengansepta interlobaris yang jelas, tetapi batas antara bagianendokrin (pulau Langerhans) dan bagian eksokrin tidak jelaspada domba. Sebaliknya pankreas kambing mempunyaibagian endokrin yang jelas batasnya dengan bagian eksokrin.Pulau Langerhans tersebar diantara eksokrin pankreas,dengan frekuensi terbanyak didapatkan pada pankreas bagiankanan (head), diikuti bagian kiri (tail) dan tengah (body).Pankreas kambing mempunyai bagian endokrin yang lebihbanyak dibanding dengan pankreas domba. Sel-sel penghasilhormon glukagon pada pankreas berbentuk polimorfik, bulat,oval, segitiga atau seperti tetes air dengan butir-butirsitoplasma yang terletak bipolar. Sel-sel ini berdistribusipada bagian perifer dari pulau Langerhans. Jumlah sel-selglukagon berbanding lurus dengan jumlah pulau Langerhanspada pankreas. Perbedaan yang diamati, mencakupperbedaan morfologis, sebaran serta jumlah pulauLangerhans dan sel-sel glukagon, sangat mungkindisebabkan oleh perbedaan dalam jenis dan pola makankedua hewan tersebut.

Morphology and distribution of gut endocrine cells in the gastrointestinal tract of Indonesia native chicken

Media Veteriner Vol 6, No 4 (1999): Media Veteriner
Publisher : Media Veteriner

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Abstract

The morphology and the distribution of gut endocrine cells in the gastrointestinal tract of Indonesian native chicken were studied using Grimelius staining method. The endocrine cells were polymorph, round oval, triangular or pyramidal in shapes and scattered among the cells in the mucosal epitheli-um and glands of all portions of the gastrointestinal tract from stomach to rectum. These cells were characterized by the presence of basally located cytoplasmic granules that react positively with the silver granules of the staining method. Two types of endocrine cells were observed in this stu-dy included open type and closed type. In the open type cells cytoplasmic elongation reached the intestinal or glandular lumen. Closed type cells possessed no such elongation but there was cytoplasmic processes run in the basal membrane. Open cells were largely distributed in the intestine while closed type cells with basally cytoplasmic processes were numerous in the gizzard. In general the endocrine cells were numerous in the jejunum of the small intestine. In the distal portion of large intestine we found clusters of endo-crine cells in the glands. The morphology and distribution pattern observed was discussed in relation with their possible functional implications.

Three Dimentional Architecture of The Subepithelial Connective Tissue in The Forestomach of The Lesser Mouse Deer (Tragulus javanicus)

Media Veteriner Vol 6, No 4 (1999): Media Veteriner
Publisher : Media Veteriner

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Abstract

Three dimensional architecture of the sub epithelial connective tissue in the forestomach of the lesser mouse deer was studied by scanning electron microscopy after macerated with 10% NaOH. In general, the architecture of the connective tissue in the rumen and reticulum showed similar pattern, which was honeycomb like pattern. This pattern was observed in all portions of the forestomach. Primary wall bordered each cell of the honeycomb. Inside the cells there were some secondary or tertiary wall that connect to the primary ones. The primary wall in the rumen were leaflike shaped with narrow and irregular surface while those of the reticulum were low columnar shaped with convex surface. The honeycomb in the ruminal papillae showed similar pattern from the apical to the basal portion. On the contrary, cone-like primary wall without secondary or tertiary ones dominated the apical portion of the reticulum papillae. The sub epithelial connective tissue consisted of collagen fibers which were arranged and formed a network. The collagen fibers in the rumen were more densely distributed as compared to those of the reticulum. The differences observed may indicate a difference in function between the rumen and the reticulum in the digestive function of the lesser mouse deer.

Tinjauan Makroskopik Organ Reproduksi Jantan Musang Luak (Paradoxurus hermaphroditus)

ACTA VETERINARIA INDONESIANA - Indonesian Veterinary Journal Vol 2, No 1 (2014): Januari 2014
Publisher : Bogor Agricultural University

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Abstract

Penelitian ini bertujuan untuk mempelajari makroanatomi organ reproduksi jantan musang luak(Paradoxurus hermaphroditus). Pengamatan morfometri meliputi pengukuran panjang, diameter,dan bobot dari organ reproduksi. Data dianalisis secara deskriptif. Hasil pengamatan menunjukkanpanjang, diameter, dan bobot testis tanpa scrotum berturut-turut adalah 2,14 cm, 1,55 cm, dan 2,85 cm.Epididimis terdiri atas kaput, korpus, dan kauda epididimis. Kelenjar prostat mempunyai panjang 3,14cm, lebar 1,82 cm dan berat 4,21 g. Penis musang luak bertipe muskulo-kavernosus dengan panjang 7,77cm dan mempunyai penis spine. Secara umum, gambaran makroanatomi organ reproduksi musang luakjantan mirip dengan hewan karnivora lain seperti anjing dan kucing.

Anatomi Organ Reproduksi Jantan Trenggiling (Manis javanica)

P-ISSN 2337-3202
Publisher : Bogor Agricultural University

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Abstract

Organ reproduksi trenggiling merupakan hal yang penting dalam menunjang upaya konservasi, karena trenggiling termasuk dalam kategori endangered species oleh IUCN dan dilindungi pemerintah berdasarkan UU No. 5/1990 serta PP No. 7/1999, meskipun menurut CITES termasuk appendix II. Penelitian ini bertujuan untuk mempelajari makroanatomi organ reproduksi jantan trenggiling (M. javanica). Organ reproduksi jantan dari lima ekor trenggiling digunakan pada penelitian ini. Pengamatan dilakukan terhadap posisi in situ, morfologi dan morfometri, yang meliputi pengukuran panjang, lebar atau diameter, tebal, dan dari masing-masing bagian organ reproduksi jantan trenggiling dengan menggunakan kaliper dalam satuan cm, serta bobot dalam satuan gr. Analisis dilakukan secara deskriptif. Hasil pengamatan menunjukkan bahwa trenggiling memiliki sepasang organ reproduksi yang terdiri atas testes, epididymis dan ductus deferens yang selanjutnya bermuara ke urethra.Testes terletak di subcutanea daerah inguinales, serta tidak terbungkus oleh scrotum. Testis dexter dan sinister memiliki bentuk dan ukuran yang relatif sama. Ukuran rata-rata testis adalah panjang 3,78 ± 0,12 cm, lebar 1,24 ± 0,02 cm, tebal 0,90 ± 0,03 cm, dan bobot 5,64 ± 0,04 g. Epididymis membentuk caput, corpus dan cauda dengan panjang rata-rata 4,78 ± 0,02 cm, sedangkan panjang rata-rata ductus deferens adalah 8,98 ± 0,31 cm. Penis berukuran kecil dan pendek, bertipe muscolocavernosus dengan rata-rata panjang dan diameter adalah 5,39 ± 1,63 cm, dan 0,64 ± 0,03 cm. Ditemukannya testes ascrotalis di subcutanea daerah inguinales merupakan hasil yang menarik dari penelitian ini yang diduga terkait dengan perilaku trenggiling menggulung tubuh.Kata kunci: trenggiling (M. javanica), organ reproduksi jantan, testes ascrotalis

MORFOLOGI DAN HISTOKIMIA KELENJAR MANDIBULARIS WALET LINCHI (Collocalia linchi) SELAMA SATU MUSIM BERBIAK DAN BERSARANG (Morphological and Histochemical Properties of Mandibular Glands of the Cave Swiflets (Collocalia linchi) During Reproductive and Nesting Period)

Jurnal Kedokteran Hewan Vol 4, No 1 (2010): J. Ked. Hewan
Publisher : Syiah Kuala University

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Abstract

The aimed of present study is to investigate the morphological and histochemical of mandibular glands of the cave swiflet (Collocalia linchi). The study used 24 adult wallet linchii paired mandibular gland located in the ventral of the mandible. They were ovoid in form and whitish in color. The gland consisted of mucous acinar cells and waspositive with PAS but negative with AB (pH 2,5). The result suggested that the acinar cells of the mandibular gland contained only neutral mucopolysaccharides and no acid mucopolysaccharides. Staining with 7 biotinylated lectins, Con-A, DBA, WGA, RCA, PNA, SBA, and UEA which represent carbohydrates with galactosa-, Nacetylgalactosamine,sialic acid, 2-5 N-acetylglucosamine, á-D- mannose, showed various positive reaction in the secretion of the acinar cells depends on the type of lectin and sampling period. The result suggested possiblecorrelation between receptor gonadal hormone with the activity of reproductive and nesting period of walet linchi.Keywords: lectin, Collocalia linchi,salivary gland.

Morphological Study of the Oesophagus and Stomach of the Cave Swiflets (Collocalia linchi)

Jurnal Kedokteran Hewan Vol 3, No 1 (2009): J. Ked. Hewan
Publisher : Syiah Kuala University

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Abstract

This study was conducted with aim to tend out morphology of oesophagus and stomach of the cave swiflets (Collocalia linchi) at macroscopic and microscopic levels.  The data revealed that esophagus was 2.93 cm in length and possessed no crop.  The stomach was small and the isthmus was not clear. Mucosa of the esophagus was lined by stratified squoamus epithelium. Esophageal gland was mucous type. The glands were well developed and distributed along the esophagus. The external muscle layer consisted of inner circular and outer longitudinal layers.  The stomach could be distinguished into proventriculus and ventriculus with no clear isthmus between them. The mucosa of proventriculus was lined by single columnar epithelium. The ventricular gland area was divided into cardiac, fundic  and pyloric gland areas. The surface of ventriculus was lined with cuticula. In general the esophagus and stomach of the cave swiftlets were simple with no crop in the esophagus and isthmus in the stomach. These might be related with the kind of food and feeding behavior of this species.

CHARACTERISTIC OF SKIN MORPHOLOGY OF SUNDA PORCUPINE (Hystrix javanica) WITH SPECIAL REFERENCE TO THE CONNECTIVE TISSUE

Jurnal Kedokteran Hewan Vol 12, No 1 (2018): J. Ked. Hewan
Publisher : Syiah Kuala University

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Abstract

This study was conducted to investigate the histological characteristic, type, and distribution of connective tissue in Sunda porcupine skin. The investigation was carried out in three adult of sunda porcupines at microscopic level using hematoxylin eosin, Masson thrichrome, Verhoeffs van Gieson, alcian blue pH 2.5 and periodic acid Schiff staining methods. Skin consists of epidermis, dermis hypodermis, and subcutaneous muscle. Quill follicles were the main and dominant structure as well as the specific characteristic on Sunda porcupine skin. The connective tissue was distributed well in basal membrane, dermis, quill follicle, and hypodermis with various intensity and density. The collagen was the main fiber found in the skin while the elastin fiber was not observed. The acid carbohydrate was found distributed well in the skin while the neutral carbohydrate was not detected in this study. In addition the fibers of connective tissue associated with the adipose tissue which found plentifully in quill follicles and hypodermis. The present results showed that the wide distribution of connective tissue might have an important role on the wound healing physiology of Sunda porcupine skin.

SEBARAN GLYCOCONJUGATE PADA SEL EPITEL OVIDUK KANCIL (Tragulus javanicus)

Jurnal Kedokteran Hewan Vol 8, No 2 (2014): J. Ked. Hewan
Publisher : Syiah Kuala University

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Abstract

Penelitian ini bertujuan mengetahui distribusi glycoconjugate yang terekspresi pada sel epitelium oviduk kancil (Tragulus javanicus). Dalam penelitian ini digunakan satu oviduk kancil yang berasal dari satu ekor kancil b etina dewasa berumur lebih dari satu tahun. Sampel difiksasi dengan larutan Bouin dan diproses menurut standar histologi sampai menjadi blok parafin dan dipotong dengan ketebalan 5 µm. Jenis lektin yang digunakan adalah biotinylated (Con A, PNA, RCA, UEA I, dan WGA) dengan dosis masing-masing sebanyak 15 µg/ml. Hasil penelitian diketahui bahwa glycoconjugate dengan residu gula galaktosa, glukosa, manosa, N-asetilgalaktosamin, N-asetilglukosamin, fukosa, dan asam sialat ditemukan pada bagian apikal sel epitel dan di dalam sitoplasma. Glycoconjugate dengan residu gula N-asetilgalaktosamin merupakan glycoconjugate yang paling banyak ditemukan di bagian apikal sel epitel dan di dalam sitoplasma dibandingkan dengan glycoconjugate dengan residu gula lainnya.