Susan Maphilindawati Noor
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

Published : 12 Documents
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Brucellosis: An Unrecognized Zoonotic Disease in Indonesia

WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences Vol 16, No 1 (2006): MARCH 2006
Publisher : Indonesian Center for Animal Research and Development

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Abstract

Brucellosis, a bacterial zoonosis, is a disease caused by members of the genus Brucella . In Indonesia, brucellosis has known as a contagious reproductive disease in animals, however, only certain people know that brucellosis can be transmitted to human . Bovine brucellosis is characterised by one or more of the following signs : abortion, retained placenta . orchitis, epididymitis and, rarely, arthritis, with excretion of the organisms in uterine discharges and in milk . The Brucella organism is transmitted to human most commonly by ingestion of untreated milk or milk products or through the mucous membranes and wound of the skin . The severity of human disease varies, depending largely upon the infecting strain . Brucella abortus, B . melitensis. B . suis and B. canis are highly pathogenic for humans . Clinical symptoms of human brucellosis may include an intermittent fever, headaches, weakness, arthalgia, myalgia and weight loss . Occasional complications include arthritis, endocarditis, hepatitis granuloma, meningitis, orchitis dan osteomyelitis have also been reported . Brucellosis can also produce spontaneous abortion in pregnant woman . Diagnosis is based on the isolation of the organism and serology . Antibiotics are usually the mainstay of treatment and long-term treatment may be required . Brucellosis can be controlled by comprehensive campaigns to eradicate the disease by vaccination programme followed by test and slaughter of domestic animals which exhibit positive serologic reactions to brucellae.   Key words : Brucellosis, zoonosis, diagnose, control

The mucosal and systemic immune responses in chickens orally immunised with Campylobacter jejuni antigen entrapped in poly-lactide-co-glycolide microparticles

Jurnal Ilmu Ternak dan Veteriner Vol 3, No 4 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

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Abstract

An oral immunisation in chickens with antigen entrapping in biodegradable microparticles was evaluated in order to achieve optimal antibody responses following oral vaccination. This study was adapted to Campylobacter jejuni antigen in chickens to observe its stimulation both mucosal and systemic immune responses. A group of 5 embryonated chicken eggs was immunised with heat-killed C. jejuni entrapped in poly-lactide-co-glycolide (PLG) microparticles at day 17 of incubation deposited into the amniotic fluid. Seven days after hatching the chickens were oral boostered, these was design to as Group A. In the Group B, each embryonated egg was immunised with soluble C. jejuni as in the same as Group A. Immune responses of post vaccination were observed at day-14, the humoral immunity was evaluated with an ELISA and whereas mucosal antibody response was detected by fluorescent histology. The serum IgG and IgA antibody responses, and also the bile and intestinal scrapping IgA antibody responses to campylobacter in Group A were significantly higher than those of the soluble antigen of Group B (P<0.05). Total number of immunoglobulin-containing cells for IgG, IgM, and IgA isotypes in the ileum in Group A chickens were also significantly higher than those of Group B (P<0.05) but was not in the duodenum and spleen. Key words : Campylobacter jejuni, poly-lactide-co-glycolide microparticles, oral immunisation, chickens

DNA Amplification Technique for Detection of Bovine Brucellosis

WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences Vol 28, No 2 (2018): June 2018
Publisher : Indonesian Center for Animal Research and Development

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Abstract

Brucellosis is one of cattle diseases which causes a very significant economic loss and categorized as zoonotic disease. Early detection of Brucellosis in livestock is very important to prevent the spread of disease to livestock and humans. The success of Brucellosis control depends on rapid, sensitive and specific detection methods. The aim of this paper is to review several methods of Brucellosis detection in cattle. Currently, the detection of Brucellosis in Indonesia is using serological and isolation methods. The latter method is the gold standard of Brucellosis diagnosis, however, its sensitivity is low. Therefore, molecular techniques with DNA amplification have been developed and applied in many countries both in livestock and humans because they are more sensitive, specific and rapid in detecting Brucella sp in blood, milk and semen samples. Various DNA amplification methods for detection of Brucellosis that have been developed including polymerase chain reaction (PCR), finger printing and loop-mediated isothermal amplificatiom (LAMP). Both PCR and LAMP are more sensitive and specific in detecting Brucella sp than conventional techniques. PCR technique has advantages in detecting Brucella sp species to serotype and biovar levels. In addition, PCR reagents are cheaper and easier to obtain than LAMP eventhough, LAMP procedure is simpler and faster.

Identifikasi Brucella abortus Isolat Lokal dengan Brucella abortus Strain Specific-Polymerase Chain Reaction (IDENTIFICATION OF LOCAL ISOLATES OF BRUCELLA ABORTUS USING BRUCELLA ABORTUS STRAIN SPECIFIC-POLYMERASE CHAIN REACTION ASSAY)

Jurnal Veteriner Vol 15, No 3 (2014)
Publisher : Jurnal Veteriner

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Abstract

Brucella abortus Strain Specific-Polymerase Chain Reaction (BaSS-PCR) is a single multiplex PCRtechnique which able to identify and differentiate between Brucella abortus field strains (biovar 1, 2, and4), B. abortus vaccine strains, Brucella species, and non-Brucella species. In this study, BaSS-PCR wasapplied to identify local isolates of B. abortus in order to investigate the B. abortus strains that infectedcattle in Indonesia. Fifty local strains of B.abortus isolated from infected cattle in Java (Jakarta andBandung), South Sulawesi (Maros), East Nusa Tenggara (Kupang and Belu) were used in this study. TheDNA bands were observed by agarose gel in the presence of ethidium bromide. Identification was performedbased on the size and number of DNA products amplified by PCR from each isolates. The results showedthat the 50 isolates were of B. abortus field strains. This finding showed that the cause of bovine brucellosisin Indonesia is B. abortus field strains.

The mucosal and systemic immune responses in chickens orally immunised with Campylobacter jejuni antigen entrapped in poly-lactide-co-glycolide microparticles

Indonesian Journal of Animal and Veterinary Sciences Vol 3, No 4 (1998)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Original Source | Check in Google Scholar | Full PDF (157.249 KB)

Abstract

An oral immunisation in chickens with antigen entrapping in biodegradable microparticles was evaluated in order to achieve optimal antibody responses following oral vaccination. This study was adapted to Campylobacter jejuni antigen in chickens to observe its stimulation both mucosal and systemic immune responses. A group of 5 embryonated chicken eggs was immunised with heat-killed C. jejuni entrapped in poly-lactide-co-glycolide (PLG) microparticles at day 17 of incubation deposited into the amniotic fluid. Seven days after hatching the chickens were oral boostered, these was design to as Group A. In the Group B, each embryonated egg was immunised with soluble C. jejuni as in the same as Group A. Immune responses of post vaccination were observed at day-14, the humoral immunity was evaluated with an ELISA and whereas mucosal antibody response was detected by fluorescent histology. The serum IgG and IgA antibody responses, and also the bile and intestinal scrapping IgA antibody responses to campylobacter in Group A were significantly higher than those of the soluble antigen of Group B (P<0.05). Total number of immunoglobulin-containing cells for IgG, IgM, and IgA isotypes in the ileum in Group A chickens were also significantly higher than those of Group B (P<0.05) but was not in the duodenum and spleen. Key words : Campylobacter jejuni, poly-lactide-co-glycolide microparticles, oral immunisation, chickens

Brucellosis: An Unrecognized Zoonotic Disease in Indonesia

Indonesian Bulletin of Animal and Veterinary Sciences Vol 16, No 1 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Original Source | Check in Google Scholar | Full PDF (787.624 KB)

Abstract

Brucellosis, a bacterial zoonosis, is a disease caused by members of the genus Brucella . In Indonesia, brucellosis has known as a contagious reproductive disease in animals, however, only certain people know that brucellosis can be transmitted to human . Bovine brucellosis is characterised by one or more of the following signs : abortion, retained placenta . orchitis, epididymitis and, rarely, arthritis, with excretion of the organisms in uterine discharges and in milk . The Brucella organism is transmitted to human most commonly by ingestion of untreated milk or milk products or through the mucous membranes and wound of the skin . The severity of human disease varies, depending largely upon the infecting strain . Brucella abortus, B . melitensis. B . suis and B. canis are highly pathogenic for humans . Clinical symptoms of human brucellosis may include an intermittent fever, headaches, weakness, arthalgia, myalgia and weight loss . Occasional complications include arthritis, endocarditis, hepatitis granuloma, meningitis, orchitis dan osteomyelitis have also been reported . Brucellosis can also produce spontaneous abortion in pregnant woman . Diagnosis is based on the isolation of the organism and serology . Antibiotics are usually the mainstay of treatment and long-term treatment may be required . Brucellosis can be controlled by comprehensive campaigns to eradicate the disease by vaccination programme followed by test and slaughter of domestic animals which exhibit positive serologic reactions to brucellae.   Key words : Brucellosis, zoonosis, diagnose, control

Study in ovo immunisation with flagellin and whole cell protein antigens of Campylobacter jejuni in chickens

Indonesian Journal of Animal and Veterinary Sciences Vol 5, No 2 (2000)
Publisher : Indonesian Animal Sciences Society

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Abstract

In ovo immunisation of chickens with flagellin and whole cell protein antigens of Campylobacter jejuni was examined to determine Campylobacter infection. Four groups of embryonated chicken eggs (10 eggs per group) were immunised in ovo at day 17 of incubation and booster was given at 7 days post-hatch. Group I was immunised in ovo and oral booster with whole cell protein of C. jejuni, group II was immunised in ovo and oral booster with C. jejuni flagellin protein, group III was immunised in ovo and intraperitoneal booster with whole cell, and group IV was treated as control. The humoral immune responses were determined by enzyme-linked immunosorbent assay (ELISA) and the mucosal immune responses were examined by a direct fluorescent histology antibody technique. Immunised chickens of Group I, II, and III shown to have higher antibody titers than  those of control chickens (group IV). The titres of anti-campylobacter antibodies of all isotypes in serum, bile, and intestinal scrapping after challenge were not significantly different in all groups. In addition, when immunised chickens were orally challenged with a homologous strain of viable C. jejuni organism, the chickens remained infected throughout the experiment based on cloacal swabs and caecal contents. These findings indicated that although in ovo immunisation resulted in increasing of the mucosal and humoral immune responses in chickens, it is not strong enough to protect the Campylobacter colonisation in the intestinal tract.   Key words : In ovo immunisation, Campylobacter jejuni, whole cell, flagellar, chickens

Efektivitas Ekstrak Biji Mangga Harumanis terhadap Staphylococcus aureus, Bacillus subtilis, Shigella sp., dan Escherichia coli (EFFECTIVITY OF MANGO HARUMANIS SEED EXTRACT TO STAPHYLOCOCCUS AUREUS, BACILLUS SUBTILIS, SHIGELLA SP., AND ESCHERICHIA COLI)

Jurnal Veteriner Vol 17, No 1 (2016)
Publisher : Jurnal Veteriner

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Abstract

Mango (Mangifera indica L.) is a tropical fruit trees in the flowering plant. Mango has been reportedas an antibacterial. The experiment was conducted to analysis the secondary compounds in seed Mangiferaindica L. extract and to test the antibacterial of seed Mangifera indica L. Staphylococcus aureus, Bacillussubtilis, Shigella sp and Escherichia coli. The plants material was extracted by percolation with ethanol.The assays were performed by using paper diffusion for determination of inhibition zone and dilutionmethod for determination of minimum inhibitory concentration. The result showed that secondarycompounds in seed mango extract were flavonoid, triterpenoid, saponin. Seed mango extract hasantibacterial activity on Staphylococcus aureus, Bacillus subtilis, Shigella sp and Escherichia coli.

Study in ovo immunisation with flagellin and whole cell protein antigens of Campylobacter jejuni in chickens

Jurnal Ilmu Ternak dan Veteriner Vol 5, No 2 (2000): JUNE 2000
Publisher : Indonesian Animal Sciences Society

Show Abstract | Original Source | Check in Google Scholar | Full PDF (161.048 KB)

Abstract

In ovo immunisation of chickens with flagellin and whole cell protein antigens of Campylobacter jejuni was examined to determine Campylobacter infection. Four groups of embryonated chicken eggs (10 eggs per group) were immunised in ovo at day 17 of incubation and booster was given at 7 days post-hatch. Group I was immunised in ovo and oral booster with whole cell protein of C. jejuni, group II was immunised in ovo and oral booster with C. jejuni flagellin protein, group III was immunised in ovo and intraperitoneal booster with whole cell, and group IV was treated as control. The humoral immune responses were determined by enzyme-linked immunosorbent assay (ELISA) and the mucosal immune responses were examined by a direct fluorescent histology antibody technique. Immunised chickens of Group I, II, and III shown to have higher antibody titers than  those of control chickens (group IV). The titres of anti-campylobacter antibodies of all isotypes in serum, bile, and intestinal scrapping after challenge were not significantly different in all groups. In addition, when immunised chickens were orally challenged with a homologous strain of viable C. jejuni organism, the chickens remained infected throughout the experiment based on cloacal swabs and caecal contents. These findings indicated that although in ovo immunisation resulted in increasing of the mucosal and humoral immune responses in chickens, it is not strong enough to protect the Campylobacter colonisation in the intestinal tract.   Key words : In ovo immunisation, Campylobacter jejuni, whole cell, flagellar, chickens

Deteksi Spesies Brucella pada Kambing di Rumah Potong Hewan Jakarta

Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

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Abstract

Brucellosis is a zoonosis and occupational diseases transmision. The diseases caused by bacterial and attack multiple species of animals. Common species that infects goats as the most pathogenic species (zoonotic) is Brucella melitensis; however, the species B. abortus could also infect goats. The study purposed to find out the brucellosis seropositive in goat in Jakarta slaughterhouse and to detect caused agent of brucellosis. Sampling was done through slaughtered goats that come from brucellosis endemic area. The samples were collected fromslaughtered mature female goats i.e serum, goat milk, vaginal swab, mamary gland, limphoglandula supramamary, limph, and uterus. The detection method was used i.e patological lession, serological, culture and PolymeraseChain Reaction (PCR) technique. The serological detection of brucellosis in goats was done parallelly between Rose Bengal Test (RBT), Complement Fixation Test (CFT) and Enzyme Linked Immunosorbent Assay (ELISA). The results of this study demonstrated that out of the 119 serum samples serologically tested, negative for RBT, one was positive for CFT and none were positive with ELISA. Patological observation in the Brucella predilection organs, there were 5 goat carcases showed pathological lession (vagina discharge, hemoragy at limphand limphoglandula, crumbly limph and there were pus in uterus). The serum samples that had reacted positively and the organs with pathological lesion were confirmed further with PCR, bacterial isolation and identification.The PCR test results and the culture of milk samples, vaginal swabs and organs did not reveal any Brucella spp bacteria (B. abortus, B. melitensis, B. ovis dan B. suis) and also vaccine strains of RB51. Based on these results, it was concluded that brucellosis in goats on Java Island was a 0.84% seropositive (confidence interval 95%; 0.00826 - 0.00854) (1/119), although the species of Brucella that had infected them remains unknown.