Ronny Rachman Noor
Departemen Ilmu Produksi dan Teknologi, Fak. Peternakan IPB, Jl. Agatis, Darmaga, Bogor

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Polimorfisme Protein Darah Itik Pegagan dengan Metode PAGE Sari, Meisji Liana; Noor, Ronny Rachman; Hardjosworo, Peni S.; Nisa, Chairun
Jurnal Agripet Vol 11, No 2 (2011): Volume 11, No. 2, Oktober 2011
Publisher : Agricultural Faculty

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Abstract

polymorphism of blood protein pegagan duck by PAGE methodABSTRACT. Pegagan duck is one of local duck species which comes from Ogan Ilir (OI) Regency, South Sumatera. This species is not popular, yet. But, it has spread out along Ogan river which comprises of three districts e.g Tanjung Raja, Inderalaya and Pemulutan. The ecosystem in this three districts is dominated by swampy area. As far, there is relatively limited information of Pegagan duck compared to another local duck. So, for the first step was done a reseach to identify genetic characteristic from blood protein analyses by electrophoresis method. It is expected that this information will become the bases in determining of the policy of Pegagan duck development. Polymorphism analyses of blood protein was done with 9 duck samples which taken randomly from 400 pegagan ducks. Protein locus observed were albumin (Alb), post albumin (Pa), transferrine (Tf), post transferrine-1 (Ptf-1), and post transferrine-2 (Ptf-2). Polymorphism analyses of blood protein of pegagan duck was characterized by allele A in locus Transferrine (TfA), Post transferrine-1 (Ptf-1A) and Post transferrine-2 (Ptf-2A) . Genetic diversity based on blood protein of Pegagan duck showed that gen frequency value in a range of 0,11 – 0,94 and high heterozygosis value that was 0,62 %.
Behaviour characteristics estimation tool of genetic distance between sheep breeds Handiwirawan, Eko; Noor, Ronny Rachman; Sumantri, Cece; ., Subandriyo .
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 4 (2014)
Publisher : Indonesian Animal Sciences Society

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Abstract

Information on the estimation of genetic distances and differentiation among sheep breeds are needed in crossing and conservation programs. This research aims to study of utilizing behaviour characteristic variables to differentiate and estimate genetic distance between the sheep breeds.  The study was conducted at Cilebut and Bogor Animal House of Indonesian Research Institute for Animal Production.  Five sheep breeds used were Barbados Black Belly Cross (BC), Garut Composite (KG), Garut Local (LG), Sumatera Composite (KS) and St. Croix Cross (SC), with total sample of 50 heads.  A total of 10 variables of behavior traits were observed in this study. Analysis of variances and significance tests were applied to compare between sheep breeds and performed for all of behavior traits using PROC GLM of SAS Program ver. 9.0. PROC CANDISC was used for canonical discriminant analyses, the hierarchical clustering was performed using the PROC CLUSTER by Average Linkage method (Unweighted Pair-Group Method Using Arithmetic Averages, UPGMA), and the dendogram for the five sheep breeds was described using PROC TREE.  The differentiator variables for the behavior traits were standing and feeding duration.  The canonical plotting based on behavioral characteristics could differentiate BC, KS and LG (with KG and SC) sheeps. Estimation of genetic distance based on the behavior traits is less accurate for grouping of sheep breeds. Key Words: Behaviour Characteristic, Differentiation, Genetic Distance
Isolation and identification of indigenous lactic acid bacteria from North Sumatra river buffalo milk Rizqiati, Heni; Sumantri, Cece; Noor, Ronny Rachman; Damayanthi, E.; Rianti, E. I.
Indonesian Journal of Animal and Veterinary Sciences Vol 20, No 2 (2015): JUNE 2015
Publisher : Indonesian Animal Sciences Society

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Buffalo milk is a source of various lactic acid bacteria (LAB) which is potential as culture starter as well as the probiotic. This study was conducted to isolate and identify LAB from indigenous North Sumatra river buffalo milk. Lactic acid bacteria was isolated and grown in medium De Man Rogosa Sharpe Agar (MRSA). The isolation was conducted to obtain pure isolate. The identification of  LAB was studied in terms of morphology, physiology, biochemistry and survival on low pH. Morphology tests were conducted by Gram staining and cell forming; physiology tests were conducted for growing viability at pH 4.5 and temperature at 45oC; whereas biochemistry tests were conducted for CO2, dextran and NH3 productions. Determination of LAB species was conducted using Analytical Profile Index (API) test CHL 50. Results of identification showed that 41 isolates were identified as LAB with Gram-positive, catalase-negative, rod and round shaped characteristics. Resistance test done to low pH (pH 2) for the lactic acid bacteria showed decrease of bacteria viability up to1.24±0.68 log cfu/ml. The resistant isolates at low pH were L12, L16, L17, L19, L20, M10, P8, S3, S19 and S20. Identification with API test CHL 50 for 10 isolates showed that four isolates were identified as Lactobacillus plantarum, L. brevis, L. pentosus and Lactococuslactis. Key Words: Buffalo Milk, LAB, Isolation, Identification
Behaviour characteristics estimation tool of genetic distance between sheep breeds Handiwirawan, Eko; Noor, Ronny Rachman; Sumantri, Cece; ., Subandriyo .
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 4 (2014): DECEMBER 2014
Publisher : Indonesian Animal Sciences Society

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Abstract

Information on the estimation of genetic distances and differentiation among sheep breeds are needed in crossing and conservation programs. This research aims to study of utilizing behaviour characteristic variables to differentiate and estimate genetic distance between the sheep breeds.  The study was conducted at Cilebut and Bogor Animal House of Indonesian Research Institute for Animal Production.  Five sheep breeds used were Barbados Black Belly Cross (BC), Garut Composite (KG), Garut Local (LG), Sumatera Composite (KS) and St. Croix Cross (SC), with total sample of 50 heads.  A total of 10 variables of behavior traits were observed in this study. Analysis of variances and significance tests were applied to compare between sheep breeds and performed for all of behavior traits using PROC GLM of SAS Program ver. 9.0. PROC CANDISC was used for canonical discriminant analyses, the hierarchical clustering was performed using the PROC CLUSTER by Average Linkage method (Unweighted Pair-Group Method Using Arithmetic Averages, UPGMA), and the dendogram for the five sheep breeds was described using PROC TREE.  The differentiator variables for the behavior traits were standing and feeding duration.  The canonical plotting based on behavioral characteristics could differentiate BC, KS and LG (with KG and SC) sheeps. Estimation of genetic distance based on the behavior traits is less accurate for grouping of sheep breeds.
Voice characteristics of some sheep: Utilization to estimation of genetic distance Handiwirawan, Eko; Noor, Ronny Rachman; Sumantri, Cece; Subandriyo, .
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 4 (2015): DECEMBER 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

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Abstract

Sound analysis has been carried out in various activities including identification and differentiation of species as well as the preparation of the taxonomy of some animals’ species because of several advantages, including no need to capture or too close to the subject observed. Analysis of voice used to differentiate and to estimate of breeds’ sheep genetic distance has not been reported. This research was conducted to study the character of a few breeds’ sheep sound and likely to be used as a predictor of genetic distance between breeds of sheep. The study was conducted in the Animal House at Indonesian Research Institute for Animal Production, Bogor.  A total of 20 head adult of five sheeps (St. Croix cross / SC, Barbados Black Belly cross / BC, Local Garut/LG, Composite Garut / KG and Composites Sumatra / KS) used in this study. Call sound recorded using a digital voice recorder. Sound analysis performed by Raven Software Pro 1.3 for Windows to count as many as 24 variables sound. Analysis of variance of each variable sound was performed using PROC GLM of SAS software Ver. 9.0. It used PROC CANDISC for canonical discriminant analysis and then PROC TREE to build a dendogram. The results showed that there were variations in amplitude, energy, power and frequency variables among the five breeds of sheep. By plotting canonical, LG, KS and BC sheep were from a different group. It was concluded that the sound characteristics variables which can be used as a differentiator breeds of sheep were the third quartile frequency, center frequency, maximum frequency and the first quartile time. Dendogram showed that KG sheep was in the less accurate group. Genetic distance estimation method using voice characteristic data may be applied on sheep. Key Words: Characteristics, Call Voice, Differentiation, Genetic Distance, Sheep
ANALISIS KEMAMPUAN DAYA GABUNG GEN PADA GENOTIPE UDANG GALAH UNTUK MENDUKUNG PROGRAM SELEKSI DAN HIBRIDISASI Hadie, Wartono; Subandriyo, Subandriyo; Hadie, Lies Emmawati; Noor, Ronny Rachman
Jurnal Penelitian Perikanan Indonesia Vol 11, No 5 (2005): (Vol. 11 No. 5 2005)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

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Abstract

Penelitian tentang daya gabung gen (conbining ability), dilakukan dengan mengunakan tiga strain udang galah yakni Musi, Barito, dan GlMacro. Perkawinan dilakukan secara fullsib di dalam dan di antara strain serta menghasilkan sembilan kombinasi genotipe.
Analisis Keterkaitan GEN BMP15, BMPR1B, dan KISS1 dengan Sifat Fekunditas pada Kambing Peranakan Etawah Betina Mulyono, Rini Herlina; Sumantri, Cece; Noor, Ronny Rachman; Jakaria, .; Astuti, Dewi Apri
Jurnal Ilmu Pertanian Indonesia Vol 24, No 2 (2019): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Etawah grade goat is a result of grading up line between Kacang and Etawah goats, which well adapted to Indonesia?s humid tropical climate. This goat is a dual-purpose goat breed for?milk?and meat?production. The objectives of this study were to evaluate the polymorphism of fecundity genes through the investigation of four candidate genes for prolificacy, i.e., BMP15 (exon 1), BMPR1B (exon 1), KISS1 (exon 1), and KISS1 (intron 1) and their associations with the litter size of Etawah-Grade does. The diversity of BMP15 (exon 1), BMPR1B (exon 1), KISS1 (exon 1), and KISS1 (introns 1) genes were analyzed by the PCR-RFLP technique. A total of 106 of DNA samples were collected from Etawah-Grade does at BPTU-HPT Pelaihari in Pelaihari, South Kalimantan (51 heads) and Cordero Farm in Bogor, West Java (55 heads). Genetic polymorphisms between the two Etawah-Grade groups were calculated as well as their allele and genotype frequencies and the Polymorphic Informative Content (PIC). The Kruskal-Wallis one-factor ranked analysis of variance was used to determine whether there was any effect of genotype of the fecundity genes on litter size. The polymorphic information content (PIC) estimated at BPTU-HPT Pelaihari was 0.313, whereas at Cordero farm was 0.174, and overall PIC was 0.244. There was no association between genotypes of BMP15 (exon 1), BMPR1B (exon 1), KISS1 (exon 1), and KISS1 (intron 1) genes and litter size. Results indicated that BMP15 (exon 1) and KISS1 (exon 1) genes were monomorphic. However, the other two loci showed polymorphisms. The BMPR1B (exon 1) locus had two genotypes with the frequency for CC (96.23%) and for GG (3.77%), and KISS1 (intron 1) locus had three genotypes with the frequency for TT (80.19%), AT (17.92%), and AA (1.89%).