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Analisis Hubungan Keragaman Gen SCD1 (Stearoyl CoA Desaturase) dengan Komposisi Asam lemak Daging Sapi Lokal di Ciamis Hilmia, Nena; Noor, R.R; Sumantri, C; Priyanto, R; E, Gurnadi
Jurnal Ilmu Ternak Universitas Padjadjaran Vol 16, No 2 (2016)
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.24198/jit.v16i2.11573

Abstract

SCD1 merupakan kandidat gen yang berperan dalam mengkonversi asam lemak jenuhmenjadi  tidak  jenuh,  dan  rasio  keduanya  menentukan  kualitas  daging.  Single  NucleotidePolymorphism (SNP) V293A pada gen SCD1 yang   mengubah asam amino Valina menjadiAlaninaberpengaruh terhadap komposisi asam lemak daging. Penelitian ini bertujuan mengidentifikasi keragaman gen SCD1 berdasarkan SNP V293A, dan hubungannya dengan kandungan  asam lemak pada sapi lokal  di Ciamis. Penelitian ini menggunakan 14  sampel daging  dan  DNA  dengan  genotipe  yang  berbeda.  Metode  PCR-RFLPdengan  enzim  Aci1digunakan untuk mendeteksi SNP V293A pada exon 5.   Hubungan keragaman gen SCD1 dengan komposisi asam lemak daging dianalisis dengan uji T. Hasil penelitian ini menunjukkan gen SCD1 pada sapi lokal Ciamis polimorfik, terdapat dua alel, yaitu alel T dan C dengan tiga genotipe,   yaitu TT, CT  dan CC.  SNP V293A pada gen SCD1 tidak  berpengaruh terhadap komposisi asam lemak daging sapi lokal di Ciamis Jawa Barat.Kata kunci : asam lemak daging, PCR-RFLP, Sapi lokal Ciamis, SCD1.
Hubungan Keragaman Gen Leptin dengan Kualitas Fisik Daging Sapi Lokal Di Ciamis (Relationship between Leptin Gene Diversity with Physical Quality of Local Beef In Ciamis) Hilmia, Nena; Noor, R.R; Sumantri, C.; Priyanto, R.; E, Gurnadi
Jurnal Ilmu Ternak Universitas Padjadjaran Vol 15, No 2 (2015)
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.24198/jit.v15i2.9528

Abstract

Leptin  terlibat  dalam  berbagai  proses  fisiologispembentukan  lemak.  Keragaman  gen Leptin  karena  adanya  SNP  Arg25Cys  pada  exon  2,  mempunyai  hubungan  dengan  deposisi lemak, yang dapat mempengaruhi  kualitas daging. Penelitian  ini bertujuan  untuk mengetahui keragaman gen leptin  dan hubungannya dengan kualitas fisik daging sapi lokal di Ciamis Jawa Barat.  Penelitian  ini  menggunakan  14  sampel  daging  bagian  udamaru  yang  diperoleh  dari peternakan  rakyat.Sampel  DNA diisolasi  dari daging.  Amplifikasi  DNA menggunakan  PCR, dan   penentuan   genotipe   dianalisis   dari   hasil   sequencing   produk   PCR.   Hasil   penelitian menunjukkan Gen Leptin pada sapi lokal Ciamis bersifat polimorfik, terdapat tiga alel, yaitu C, T (memiliki mutasi Arg25Cys) dan H (memiliki mutasi Arg25His) serta terdapat tiga genotipe CC,  CT  dan  CH.  Perbedaan  genotipe  gen  Leptin  tidak  berpengaruh  terhadap  kualitas  fisik daging sapi lokal di Ciamis.Kata kunci: Kualitas daging, Leptin
Morphometric performances of Katingan cattle in Central Kalimantan Utomo, Bambang Ngadji; Noor, R.R; Sumantri, C; Supriatna, I; Gunardi, E.D
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 3 (2010): SEPTEMBER 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.3 KB) | DOI: 10.14334/jitv.v15i3.661

Abstract

The information of Katingan cattle as an indigenous cattle in Central Kalimantan is very limited, especially phenotypic characterization. A number of 72 heads, consist of 57 heads female and 15 heads male of Katingan cattle in adults category were used in this study. The samples were obtained from three sub districts in district of Katingan, those were Tewah Sanggalang Garing (Pendahara Village), Pulau Malan (Buntut Bali Village) and Katingan Tengah (Tumbang Lahang Village). The location were used as the variable of groups (subpopulation). This study was conducted to describe morphometric performance, which was analysed by discriminant and canonical to estimate the phylogenic relationship and to determine the discriminant variable among the group (subpopulation). The SAS package program version 6.0 and MEGA 2 program was used to analyze data. The observed parameters were body length, wither height, chest width, chest depth, chest circumference, hip heigth, hip width, head height, head length and head width. The chest depth and hip height of female cattle from group of Buntut Bali were lower (P < 0.05) than that of Pendahara and Tumbang Lahang groups. The parameter of: chest width, hip width and body length of Buntut Bali groups were higher (P < 0.05) than that of Pendahara and Tumbang Lahang groups. The average body weight of male Katingan cattle from Buntut Bali was higher (P < 0.05) than that of Pendahara and Tumbang Lahang groups but there were not different (P > 0.05) on bodyweight of female Katingan cattle among location. The highest similarity value was Katingan cattle from group of Tumbang Lahang (92,86%) followed by group of Pendahara (40.91%) and group of Buntut Bali (38.89%), repectivelly. Katingan cattle from group of Pendahara and Tumbang Lahang had closest genetic distance (1.406), while the longest genetic distance (2.046) was between group of Buntut Bali and group of Tumbang Lahang. Genetic distance between group of Buntut Bali and Pendahara was 1.929. Katingan cattle from Pendahara and Tumbang Lahang within one cluster. Body length in canonical structure 2 can be used as discriminant variable. Key Words: Katingan Cattle, Morphometric, Genetic Distance, Discriminant Analysis, Canonical
Carcass and physical meat characteristics of thin tail sheep (TTS) based on calpastatin gene (CAST) (Locus intron 5 – exon 6) genotypes variation Dagong, Muhammad Ihsan Andi; Herman, R; Sumantri, C; Noor, R.R; Yamin, M
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 1 (2012): MARCH 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (159.339 KB) | DOI: 10.14334/jitv.v17i1.708

Abstract

The quality of sheep carcass is mostly determined by the total lean meat production, meat distribution on the carcass and the quality of meat. Calpastatin gene (CAST) is known to have an association with carcass and meat quality traits. The objective of this research was to identify the association between CAST polymorphisms and carcass characteristics in Thin Tail Sheep (TTS). Thirty three heads of sheep representing three genotypes of CAST (CAST-11, CAST-12 and CAST-22) were identified for carcass and meat characterisation. There was no significant difference between CAST polymorphisms with meat tenderness, pH, water holding capacity and cooking loss, neither with carcass weight and dressing percentage among genotypes. Shoulder proportion of CAST-11 genotype was larger than that of CAST-12 or CAST-22, but the lean meat proportion of CAST-22 genotype in shoulder, rack and loin were higher than those of CAST-11 but not different from CAST-12. The fat percentage of CAST-11 was the highest among the genotypes. CAST-22 genotype has higher lean meat percentage than the CAST-11. Variation in CAST gene could be used as marker assisted selection in sheep for higher lean meat proportion. Key Words: Calpastatin, Polymorphisms, Carcass Characteristics, Meat Quality, Thin Tail Sheep
Relationship of molting trait and egg production on crossbred Peking and Alabio ducks Susanti, Triana; Noor, R.R; Hardjosworo, P.S; Prasetyo, L.H
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 2 (2012): JUNE 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.392 KB) | DOI: 10.14334/jitv.v17i2.685

Abstract

Selection program through 6 months egg production record as criterion of selection can improve number of egg in a year. However the selected population has still showed molting which is influenced the cessation of egg production, so that its production has not optimal yet. The research has conducted to further learn about the relationship between molting and egg production. This study used 90 females AP ducks (the crossbred of Alabio ♂ and Peking ♀) and 90 females PA ducks (the crossbred of Peking ♂ and Alabio ♀). Observed variables were onset of molting, the length of molting periode and the egg production for 48 weeks. The collected data were analyzed by analysis of variance, correlation and regression. The results showed that based on the starting time of molting the ducks can be categorized into two group i.e (a) molting duck group and (b) non-molting duck group. There were 63 PA ducks characterized non-molting and 21 molting, and there were 42 non-molting and 45 molting in AP ducks. The egg production of these two groups was significantly different (P < 0.01). Since the molting was negatively correlated to the 48 weeks egg production (r = -0.896 for PA and -0.553 for AP), then this trait can be used as the selection criterion for egg production.The linear regression equation for PA of 48 weeks egg production = 80.7-0.369 molting duration, and for AP of 48 weeks egg production = 84 - 0.299 molting duration. Key Words: Molting, Egg Production, Duck
Growth curve analysis of Garut sheep and its crossbreds Inounu, Ismeth; Mauluddin, D; Noor, R.R; ., Subandriyo
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 4 (2007): DECEMBER 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (176.486 KB) | DOI: 10.14334/jitv.v12i4.496

Abstract

Data of body weight of Garut sheep and its crossbreds of 488 head, consist of  149 head of Garut sheep, 115 head of St. Croix X Garut (HG), 68 head of Mouton Charollais X Garut (MG), 101 head of MG X HG (MHG) and 55 head of HG X MG (HMG) which is collected from Indonesian Research Institute for Animal Production at Bogor station were used in individual growth curve analysis. Three growth curve non linier model were used in this study i.e Logistik, Gompertz and Von Bertalanffy models. Comparisons were made among these models for goodness of fit, biological interpretability of parameters and computional ease and effect of genotype and environment in them. Least square means growth curve parameters which have biological interpretability were used to compare effect the genotype, interse mated procces and estimated heterosis effect. The result indicated that Von Bertalanffy was the best model in fitting the data from Garut and Crossbreds although the model needed more iteration than others in computations. All models have good biological interpretability especially for parameter mature size (A) and rate of maturity (k). Genotype, year of birth, sex and type of birth reared were important effects (p<0.01) in mature size (A) for all models except effect of type of birth rearing (P<0.05) in Logistic model. Year of birth had important effect (p<0.01) in rate of maturity (k) for all models. Genotype, year of birth, sex, parity and type of birth reared also had important effect (p<0.01) in parameter b/M; except b parameter in Von Bertalanffy was affected significantly by sex (P<0.05). Key Words: Growth Curve, Garut Sheep and Crossbreds, Relative Superiority
Growth curve analysis of Garut sheep and its crossbreds Inounu, Ismeth; Mauluddin, D; Noor, R.R; ., Subandriyo
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 4 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v12i4.496

Abstract

Data of body weight of Garut sheep and its crossbreds of 488 head, consist of  149 head of Garut sheep, 115 head of St. Croix X Garut (HG), 68 head of Mouton Charollais X Garut (MG), 101 head of MG X HG (MHG) and 55 head of HG X MG (HMG) which is collected from Indonesian Research Institute for Animal Production at Bogor station were used in individual growth curve analysis. Three growth curve non linier model were used in this study i.e Logistik, Gompertz and Von Bertalanffy models. Comparisons were made among these models for goodness of fit, biological interpretability of parameters and computional ease and effect of genotype and environment in them. Least square means growth curve parameters which have biological interpretability were used to compare effect the genotype, interse mated procces and estimated heterosis effect. The result indicated that Von Bertalanffy was the best model in fitting the data from Garut and Crossbreds although the model needed more iteration than others in computations. All models have good biological interpretability especially for parameter mature size (A) and rate of maturity (k). Genotype, year of birth, sex and type of birth reared were important effects (p<0.01) in mature size (A) for all models except effect of type of birth rearing (P<0.05) in Logistic model. Year of birth had important effect (p<0.01) in rate of maturity (k) for all models. Genotype, year of birth, sex, parity and type of birth reared also had important effect (p<0.01) in parameter b/M; except b parameter in Von Bertalanffy was affected significantly by sex (P<0.05). Key Words: Growth Curve, Garut Sheep and Crossbreds, Relative Superiority
The relative superiority of milk production of Garut sheep and its crossbred Inounu, I; Sukmawati, S; Noor, R.R
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 4 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v11i4.541

Abstract

Garut sheep (GG) is one of some native sheep that had been well known to the people of West Java. This sheep have some advantages including their ability to produce multiple birth, reach sexual maturity faster and resistant to internal parasite. However, this sheep have also some disadvantages including low milk production, high mortality and low weaning weight. Crossbreeding is one way to improve animal genetic quality. The objective of this study is to evaluate whether the Garut, St.Croix cross (HG), and Moulton Charollais cross (MG) are superior in milk production when compared to Garut sheep. This study was conducted at small ruminant experimental station of Research Institute for Animal production from June to August 2002.  The superiority of the crossed sheep was determined by subtracting the average milk production of the crossed sheep (HG or MG) and Garut sheep and then divided the values by the average milk production of Garut sheep, except for the threeway crosses (MHG and HMG) is calculated from the difference in milk production between the means of threeway crossbred with the means of two parents (MG and HG). The data had been corrected by parity and type of birth.  The General Linear model of SAS was used to calculate the least square means. Average milk production from GG, MG, HG, MHG and HMG ewes in this study were respectivelly 53.41, 59.48, 55.89, 44.87 and 54.66 kg. The relative superiority for milk production of MG and HG were 11% and 5% over Garut sheep respectively and for the threeway crossbred MHG/HMG  was -14% over their parents (MG and HG). Key Words: Milk Production, St.Croix, Moulton Charollais, Garut, Crossing
Morphometric performances of Katingan cattle in Central Kalimantan Utomo, Bambang Ngadji; Noor, R.R; Sumantri, C; Supriatna, I; Gunardi, E.D
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 3 (2010)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v15i3.661

Abstract

The information of Katingan cattle as an indigenous cattle in Central Kalimantan is very limited, especially phenotypic characterization. A number of 72 heads, consist of 57 heads female and 15 heads male of Katingan cattle in adults category were used in this study. The samples were obtained from three sub districts in district of Katingan, those were Tewah Sanggalang Garing (Pendahara Village), Pulau Malan (Buntut Bali Village) and Katingan Tengah (Tumbang Lahang Village). The location were used as the variable of groups (subpopulation). This study was conducted to describe morphometric performance, which was analysed by discriminant and canonical to estimate the phylogenic relationship and to determine the discriminant variable among the group (subpopulation). The SAS package program version 6.0 and MEGA 2 program was used to analyze data. The observed parameters were body length, wither height, chest width, chest depth, chest circumference, hip heigth, hip width, head height, head length and head width. The chest depth and hip height of female cattle from group of Buntut Bali were lower (P < 0.05) than that of Pendahara and Tumbang Lahang groups. The parameter of: chest width, hip width and body length of Buntut Bali groups were higher (P < 0.05) than that of Pendahara and Tumbang Lahang groups. The average body weight of male Katingan cattle from Buntut Bali was higher (P < 0.05) than that of Pendahara and Tumbang Lahang groups but there were not different (P > 0.05) on bodyweight of female Katingan cattle among location. The highest similarity value was Katingan cattle from group of Tumbang Lahang (92,86%) followed by group of Pendahara (40.91%) and group of Buntut Bali (38.89%), repectivelly. Katingan cattle from group of Pendahara and Tumbang Lahang had closest genetic distance (1.406), while the longest genetic distance (2.046) was between group of Buntut Bali and group of Tumbang Lahang. Genetic distance between group of Buntut Bali and Pendahara was 1.929. Katingan cattle from Pendahara and Tumbang Lahang within one cluster. Body length in canonical structure 2 can be used as discriminant variable. Key Words: Katingan Cattle, Morphometric, Genetic Distance, Discriminant Analysis, Canonical
Relationship of molting trait and egg production on crossbred Peking and Alabio ducks Susanti, Triana; Noor, R.R; Hardjosworo, P.S; Prasetyo, L.H
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 2 (2012)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v17i2.685

Abstract

Selection program through 6 months egg production record as criterion of selection can improve number of egg in a year. However the selected population has still showed molting which is influenced the cessation of egg production, so that its production has not optimal yet. The research has conducted to further learn about the relationship between molting and egg production. This study used 90 females AP ducks (the crossbred of Alabio ♂ and Peking ♀) and 90 females PA ducks (the crossbred of Peking ♂ and Alabio ♀). Observed variables were onset of molting, the length of molting periode and the egg production for 48 weeks. The collected data were analyzed by analysis of variance, correlation and regression. The results showed that based on the starting time of molting the ducks can be categorized into two group i.e (a) molting duck group and (b) non-molting duck group. There were 63 PA ducks characterized non-molting and 21 molting, and there were 42 non-molting and 45 molting in AP ducks. The egg production of these two groups was significantly different (P < 0.01). Since the molting was negatively correlated to the 48 weeks egg production (r = -0.896 for PA and -0.553 for AP), then this trait can be used as the selection criterion for egg production.The linear regression equation for PA of 48 weeks egg production = 80.7-0.369 molting duration, and for AP of 48 weeks egg production = 84 - 0.299 molting duration. Key Words: Molting, Egg Production, Duck