Tongku Nizwan Siregar
Laboratorium Reproduksi Fakultas Kedokteran Hewan Universitas Syiah Kuala, Banda Aceh

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Tampilan Reproduksi Kambing Betina Lokal yang Induksi Berahinya Dilakukan dengan Sistem Sinkronisasi Singkat

Jurnal Veteriner Vol 11, No 1 (2010)
Publisher : Jurnal Veteriner

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Abstract

The aim of this study is to determine the reproduction performance of local does in which theirestrous was induced by short synchronization system. In this study 10 healthy female, unpregnant andhave relatively homogenous body weights were used. All does were devided into 2 experiment groups.Group 1 consisted of 4 does as control and group II consisted of 6 does as treatment. Group I, were injectedwith 125 ?g cloprostenol intramuscular, twice in 11 days interval. Group II, was treated with shortsynchronization using 125 ?g cloprostenol intramuscular, and then followed by injection of 300 IU hCGand 0.5 mg estradiol benzoate after 12 hours later. The does were inseminated 10 hours after onset ofestrous and repeated 12 hours later. Parameters measured were oestrous percentage, pregnancy, andlitter size. Results showed that all does (100%) from both groups showed estrous. Percentage of pregnantdoes group I and II were 75.00% and 83.33% respectively, and the averages litter size were 2.0 ± 1.0 and 1.4± 0.3, respetively. Treatment with short synchronization can increase pregnancy and delivered percentagealthough litter size not affected.

PEMBERIAN EKSTRAK VESIKULA SEMINALIS MENINGKATKAN KUALITAS SPERMATOZOA TETAPI TIDAK MEMENGARUHI KONSENTRASI SPERMATOZOA DAN TESTOSTERON TIKUS PUTIH (Rattus norvegicus)

Jurnal Kedokteran Hewan Vol 8, No 2 (2014): J. Ked. Hewan
Publisher : Syiah Kuala University

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Abstract

Penelitian ini bertujuan mengetahui pengaruh pemberian ekstrak vesikula seminalis terhadap kualitas spermatozoa dan konsentrasi testosteron tikus putih (Rattus norvegicus). Dalam penelitian ini digunakan 20 ekor tikus putih jantan dewasa, galur Wistar, berumur 3 -4 bulan, berat badan 250-300 g dan dibagi menjadi 4 kelompok (K1, K2, K3, dan K4), masing-masing kelompok berturut-turut diberikan 0,2 ml NaCl fisiologis, 25 μg cloprostenol, 0,2 ml ekstrak vesikula seminalis (EVS), dan 0,4 ml EVS secara intraperitoneal. Pada akhir perlakuan, seluruh tikus dikorbankan secara dislocatio cervicalis. Selanjutnya, kauda duktus epididimis dinekropsi untuk dikoleksi spermatozoanya. Pemeriksaan kualitas spermatozoa, meliputi motilitas dan konsentrasi spermatozoa. Pemeriksaan konsentrasi testosteron serum darah dilakukan menggunakan teknik enzymelinked immunosorbant assay (ELISA). Motilitas dan konsentrasi (x10 6 ) spermatozoa pada kelompok K1; K2; K3; dan K4 masingmasing adalah 3,00±0,00; 3,20±0,28; 2,00±0,86; dan 3,20±0,28 dan 146,60±71,90; 187,80±60,80; 124,20±64,70; dan 129,40±27,07. Konsentrasi testosteron pada kelompok K1; K2; K3; dan K4 masing-masing adalah 5,53+1,75; 4,68+4,56; 2,51+1,33; dan 2,40+1,60 ng/ml. Hasil penelitian menunjukkan bahwa pemberian EVS mampu meningkatkan motilitas spermatozoa tetapi tidak memengaruhi konsentrasi spermatozoa dan testosteron serum darah tikus putih.

Characterization of Inhibin from Culture and Non Culture of Granulose Cells for Monoclonal Antibody of Inhibin Production

Jurnal Kedokteran Hewan Vol 4, No 1 (2010): J. Ked. Hewan
Publisher : Syiah Kuala University

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Abstract

This study has long-term objectives to obtain immunogenic prototype that can be used to induce multiple ovulation in goats. Working steps of this study were begun with the collection of ovarium from goats, collection of granulose cells, culture of granulose and characterization of molecular weight and isoelectric point (pI) of inhibin protein of granulose cells obtained from culture and non-culture of granulose cells, and followed by preparation of monoclonal antibody toward inhibin. The results showed that inhibin isolated either from culture or non-culture of granulose cells produced a 32 kDa band. Molecular weight of inhibin was measured by Western Blot. The 32 kDa band of SDS PAGE product appeared on Western Blot result was inhibin molecules produced by granulose cells collected fom culture and non-culture of granulose cells that can be identified by Mab-inhibin. Product of IEF gel electrophoresis suggested that inhibin molecule collected from culture of granulose cells has no charge at isoelectric points ranging from 5-6, depends on its total amino acid composition.