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Peningkatan Laju Pertumbuhan dan Kandungan Flavonoid Klon Daun Dewa (Gynura pseudochina (L.) DC) Melalui Periode Pencahayaan Ghulamahdi, Munif i; Aziz, Sandra A.; Nirwan, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 36, No 1 (2008): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

Lights are influential to increase plant flavonoids content.  The bioactives is needed as medication for human cancer.  The research objectives were to investigate  the effect of lighting periods on the growth and flavonoid content of Gynura pseudochina clones. A split plot design was used  with lighting periods as the main plots (100% light  (full light) for 4 months; 25% shading for 1 month, full light 3 months; 25% shading for 2 months, full light for 2 months; 25%  shading  for 3 months, full  light  for 1 months; 25%  shading  for 4 months; 50% shading  for 1 month, full light for  3 months; 50%  shading for 2 months, full light for 2 months; 50% shading for 3 months, full light for 1 montht; 50% shading for 4 months) and sub plots were two clones (clone 9 from in vitro culture and clone 7 from shoot cuttings). The results showed that maximum LAI (Leaf Area Index) (2.72), RGR (Relative Growth Rate) (0.062 g/g/day), LAR (Leaf Area Ratio) (168.55 cm2/g) and NAR (Net Assimilation Rate) (0.00093 g/cm2/day) were obtained from plants grown under 50% shading for 4 months, full light for 4 months, 50% shading for 2 months and  full light for 2 months, respectively. The maximum plant biomass  (90.92g) was  produced by  plants  grown  under 50%  shading  for 1 month  and full light  for 3 months, while maximum total flavonoids content (11.92%) was obtained from plants under 50% shading for 3 months  and full  light for 1 month, whereas maximum anthocyanine content from those grown under 50% shading for 1 month and full light for 3 months. Clone 9 produced LAI, LAR, and anthocyanine content higher than clone 7, but RGR, NAR, total biomass, and total flavonoid content was lower than clone 7.   Key words:  Lighting periods, growth, flavonoids, Gynura pseudochina , clones
Multiplikasi dan Pigmentasi Antosianin Daun Dewa (Gynura pseudochina (L) DC) In Vitro Nirwan, ,; Aziz, Sandra Arifin
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 34, No 2 (2006): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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Abstract

The research consisted of two experiments, they were shoot multiplications experiment and root induction with anthocyanins content experiment.  Randomized block design with two factors was used in the two experiments.  In the first experiment BAP and IAA were used which were 0, 1, 2, 3 ppm and  0, 0.5 and 1 ppm, respectively. In the second experiment IAA and sucrose were used which were 0, 0.5, 1 ppm and 30, 40, 50 and 60 g/l.  Addition of BAP 3.54 and 2.98 ppm without IAA to in vitro MS medium in the first experiment, significantly increased the number of shoots (33.21) and number of leaves (52.53)  at 5 weeks after planting.  MS medium with BAP 0.32 ppm without IAA produced maximum shoot heights (4.11 cm), while addition BAP up to 3 ppm significantly reduced number of roots and induced callus diametres (1.48 cm).  Purple pink leaf colour was produced without BAP application. In the second experiment, addition of IAA 1 ppm with sucrose 52.18 g/l and IAA 1 ppm with sucose 48.36 g/l produced maximum of shoot number (15.01 shoots) and leaf number (29.16 leaves) at 8 weeks after planting, but the size of shoot and leaf were smaller.  Maximum shoot height (10.03cm), number of root (35.4) and length of root (22.3 cm) were produced at the IAA 0.5 ppm and sucrose 30 g/l. The highest anthocyanins content (0.071 %) and number of plantlet (2.6) were produced by addition of sucrose 30 g/l. Anthocyanin and number of plantlet decreased quadratically with sucrose addition.   Key words : Anthocyanin, In Vitro, Gynura pseudochina (L.) DC
Multiplikasi dan Pigmentasi Antosianin Daun Dewa (Gynura pseudochina (L) DC) In Vitro Nirwan, ,; Aziz, Sandra Arifin
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 34, No 2 (2006): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy

Show Abstract | Original Source | Check in Google Scholar

Abstract

The research consisted of two experiments, they were shoot multiplications experiment and root induction with anthocyanins content experiment.? Randomized block design with two factors was used in the two experiments.? In the first experiment BAP and IAA were used which were 0, 1, 2, 3 ppm and? 0, 0.5 and 1 ppm, respectively. In the second experiment IAA and sucrose were used which were 0, 0.5, 1 ppm and 30, 40, 50 and 60 g/l. ?Addition of BAP 3.54 and 2.98 ppm without IAA to in vitro MS medium in the first experiment, significantly increased the number of shoots (33.21) and number of leaves (52.53) ?at 5 weeks after planting.? MS medium with BAP 0.32 ppm without IAA produced maximum shoot heights (4.11 cm), while addition BAP up to 3 ppm significantly reduced number of roots and induced callus diametres (1.48 cm).? Purple pink leaf colour was produced without BAP application. In the second experiment, addition of IAA 1 ppm with sucrose 52.18 g/l and IAA 1 ppm with sucose 48.36 g/l produced maximum of shoot number (15.01 shoots) and leaf number (29.16 leaves) at 8 weeks after planting, but the size of shoot and leaf were smaller.? Maximum shoot height (10.03cm), number of root (35.4) and length of root (22.3 cm) were produced at the IAA 0.5 ppm and sucrose 30 g/l. The highest anthocyanins content (0.071 %) and number of plantlet (2.6) were produced by addition of sucrose 30 g/l. Anthocyanin and number of plantlet decreased quadratically with sucrose addition. ? Key words : Anthocyanin, In Vitro, Gynura pseudochina (L.) DC
Peningkatan Laju Pertumbuhan dan Kandungan Flavonoid Klon Daun Dewa (Gynura pseudochina (L.) DC) Melalui Periode Pencahayaan Ghulamahdi, Munif i; Aziz, Sandra A.; Nirwan, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 36, No 1 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy

Show Abstract | Original Source | Check in Google Scholar

Abstract

Lights are influential to increase plant flavonoids content.? The bioactives is needed as medication for human cancer. ?The research objectives were to investigate ?the effect of lighting periods on the growth and flavonoid content of Gynura pseudochina clones. A split plot design was used ?with lighting periods as the main plots (100% light ?(full light) for 4 months; 25% shading for 1 month, full light 3 months; 25% shading for 2 months, full light for 2 months; 25% ?shading ?for 3 months, full ?light ?for 1 months; 25% ?shading ?for 4 months; 50% shading ?for 1 month, full light for? 3 months; 50%? shading for 2 months, full light for 2 months; 50% shading for 3 months, full light for 1 montht; 50% shading for 4 months) and sub plots were two clones (clone 9 from in vitro culture and clone 7 from shoot cuttings). The results showed that maximum LAI (Leaf Area Index) (2.72), RGR (Relative Growth Rate) (0.062 g/g/day), LAR (Leaf Area Ratio) (168.55 cm2/g) and NAR (Net Assimilation Rate) (0.00093 g/cm2/day) were obtained from plants grown under 50% shading for 4 months, full light for 4 months, 50% shading for 2 months and ?full light for 2 months, respectively. The maximum plant biomass ?(90.92g) was? produced by ?plants ?grown ?under 50% ?shading ?for 1 month ?and full light? for 3 months, while maximum total flavonoids content (11.92%) was obtained from plants under 50% shading for 3 months ?and full ?light for 1 month, whereas maximum anthocyanine content from those grown under 50% shading for 1 month and full light for 3 months. Clone 9 produced LAI, LAR, and anthocyanine content higher than clone 7, but RGR, NAR, total biomass, and total flavonoid content was lower than clone 7. ? Key words:? Lighting periods, growth, flavonoids, Gynura pseudochina , clones