Eka Noneng Nawangsih
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VPH-4 The Influence of Medium pH on Lactobacillus acidophillus Viability in Soyghurt Tested In Vitro and In Vivo Halimah, Lia Siti; Paryati, Sayu Putu Yuni; Nawangsih, Eka Noneng
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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L. acidophillus bacteria have a high tolerance for acidic atmosphere. This bacterial resistance occurs because the ability to maintain cytoplasmic pH is more alkaline than extracellular pH so that all the different proteins and enzymes in it can still work optimally. L. acidophillus bacteria have cell membranes that are more resistant to cell leakage. Cellular membranes consisting of two layers of phospholipid (lipid bilayer) which on each surface of the layer are attached to proteins and lipid bilayer glycoproteins are semipermeable, which will limit the movement of compounds in and out between the cytoplasm and the external environment. The digestive tract has a different pH, starting from the oral cavity with a pH ranging from 6-7 (interval), stomach with pH 1.5-2 (acid), and intestine with a pH of 8-8.9 (base). Many bacteria can live and have habitat in the oral and intestinal cavities, but only certain bacteria are known to survive in the stomach. The nature of bacteria, in general, does not stand in an acidic atmosphere in the stomach, but there are also pathogenic bacteria (detrimental) that are resistant to the acidic atmosphere in the stomach so that it can cause disease in the digestive system. The stomach is an organ of the digestive system which functions to digest food and as the body's defense organ. The body's defense mechanism is carried out by the stomach against bacteria, namely by removing stomach acid. The presence and endurance of L. acidophillus bacteria in the stomach have not been reported. Therefore, in this study will be tested the effect of soyghurt containing L. acidophillus bacteria on the viability of L. acidophillus bacteria in the stomach and its effect on the gastric fluid profile of male Wistar rats.
Antibodies titer of dogs immunized by anti-idiotypic vaccine detected by using enzyme linked immunosorbent assay Paryati, Sayu Putu Yuni; Soejoedono, Retno D.; Nawangsih, Eka Noneng
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Rabies control programs, including extensive vaccination with attenuated or inactivated vaccines. However, such vaccines are not without problems and can have detrimental effects. Indeed attenuated vaccines can revert to a more virulent form, and inactivated vaccines may produce serious side effects. These facts, have led to the creation of a new generation of vaccines: recombinant-DNA vaccines, synthetic peptide vaccines, and anti-idiotypic vaccines. The aim of this study is to study the result of anti-idiotypic immunization methods in dogs detected by using enzyme linked immunosorbent assay (ELISA). Anti-idiotype antibodies against rabies (Ab2) were isolated from chicken blood, separated by means of ammonium sulfate precipitation, then dialyzed using PBS pH 8.0 for 24 hours at 2 – 8 oC and purified using affinity chromatography column. Three groups of dogs were immunized, group I was immunized intramuscularly (i.m) with purified IgY, group II was immunizded oraly (p.o) with purified IgY and group III was immunized intramuscularly (i.m) with rabies viral vaccines.  The antibody response (Ab3) was detected using Agar Gel Precipitation Test (AGPT). The efficacy of Ab3 was detected using ELISA. By ELISA, the result of immunization indicated that the level of Ab3 titers of anti-idiotypic vaccine immunized dogs intramuscularly are more than 0.5 IU/ml (protective according to WHO standard), and significantly higher than oraly immunization, but it significantly lower than Ab3 titers of rabies viral vaccine immunized dogs. The conclusion of this study is intramuscularly immunization of anti-idiotypic antibodies can induce protective immune response against rabies virus, although its lower than antibodies titer of viral vaccine, it has a good prospect for vaccine development in controlling rabies