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The Resistance of Lactic Acid Bacteria Isolated From the Faeces of Broiler chicken towards the Conditions of the Broiler Digestive Tract Mujnisa, A.; Rotib, Laily A.; Djide, Natsir; Natsir, Asmuddin
JURNAL ILMU DAN TEKNOLOGI PETERNAKAN Vol 2, No 3 (2013)
Publisher : Fakultas Peternakan Universitas Hasanuddin

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Abstract

This research was conducted to determine the resistance degree of lactic acid bacteria (LAB) isolated from the faeces of broilers towards various intestinal conditions, in order to select a lactic acid bacteria to be used as prospective probiotic. Ten types of LAB bacteria (M1, M2, M3, M4, M5, M7, M8, M23 ,M26, and M28) isolated from the faeces of broiler chicken were subjected to pH2, pH3, and pH4; to 3% bile salt and to the temperatures of 30oC, 37oC and 41oC. The results of the research showed that all LAB isolates experienced the highest colony reductions at pH2, which was around 3.2 - 5.5 log units/ml. The reduction of colony numbers at pH3 was approximately 1.8-3.1 log unit/ml, and the reduction at pH4 was around 1.0 – 2.5 log unit/ml. All the LAB isolates experienced a decrease of 0.5 log unit/ml to 1.6 log unit/ml in colony numbers after bile salt exposure. The reduction of colony numbers of ten LAB isolates at the temperatures of 30oC, 37oC and 41oC was 0.6 - 1.4 log unit/ml, 0.1 – 0.5 log unit/ml and 0.7 – 1.4 log unit/ml, respectively. None of the ten isolates posses all of the expected characteristic, however based on its more superior ability to block the pathogenic microorganism as well as its ability to survive in simulated gastro intestinal tract conditions, M1 was selected as prospective probiotic.
Purine derivatives excretion and estimation of microbial protein supply on sheep receiving different protein suplements Natsir, Asmuddin
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 3 (2007)
Publisher : Indonesian Animal Sciences Society

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Abstract

The urinary excretion of purine derivative (PD), which consists of allantoin, uric acid, xanthine and hypoxanthine, was used to estimate Microbial Nitrogen (MN) supply on sheep given oaten hay as basal diet supplemented either with faba beans (faba) or barley fortified with urea (barley-urea). Three growing wethers, with average body weight of 43 ± 2.29 kg, were randomly assigned to one of three experimental diets, i.e. oaten hay without supplement (C), diet C + barley-urea (CB), and diet C + faba (CF), according to latin square design (3 x 3). Results of the experiment indicated that PD excretion for C was lower (P = 0.07) than that of CB and CF (2.84 vs 6.08, 7.67 mmol/d). Absorbed PD and estimation of MN supply for C was less (P = 0.07) than those of CB and CF, namely 1.26 vs 6.64, 8.80 mmol/d and 0.91 vs 4.8, 6.30 g N/d respectively. However, the efficiency of MN synthesis was similar, whether the calculation was based on digestible organic matter fermented in the rumen, DOMR (g MN/kg DOMR, P = 0.20) or based on N intake (g MN/g N intake, P = 0.40). In conclusion, supplementation of oaten hay with different protein supplements may improve the estimation of microbial N supply in the growing sheep but both supplements gave similar results on the estimation of MN supply. Key Words: Purine Derivative, Microbial Protein, Protein Supplement, Sheep
The effects of microwave radiation on rumen degradation characteristics of barley straw cut at two different stages of maturity Natsir, Asmuddin
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

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Abstract

A common approach for improving the nutritive value of low quality roughages and crop by-products is by pre-treatment or processing either physical, chemical, or biological treatments. Microwave radiation is one type of physical treatment that could be used to treat low quality roughages. Research was carried out to investigate  the effects of microwave radiation on the rumen degradation characteristics of barley straw obtained from two different stages of maturity. The experiment was run factorially based on completely randomized block design. The first factor was stage of maturity, straw cut during the soft elongation time (C1) and during the harvest time (C2). The second factor was levels of microwave radiation times (MWRT) (T0 = control, without MWR; T1 = MWR for 1 minute, T2 = MWR for 2 minutes). The results indicated nutritive values of barley straw obtained from C1 sampling time were significantly better than that obtained from the C2 sampling time in terms of a higher rumen degradation rate and a much greater total potential rumen degradability. In contrast, MWR did not have significant effects on the rate of degradation and total potential degradability of straw in the rumen. Key Words: Microwave Radiation, Barley Straw, Rumen Degradability
Rumen microbial protein supply as estimated from purine derivative excretion on sheep receiving faba beans (vicia faba) as supplement delivered at different feeding frequencies Natsir, Asmuddin
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 2 (2008)
Publisher : Indonesian Animal Sciences Society

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Abstract

Rapid and extensive degradation of faba beans (Vicia faba) by ruminal microbes can result in substantial and undesirable N loss from the rumen. The purpose of this study was to test the hypothesis that offering faba beans as a supplement more than once a day to sheep receiving a combination of oaten chaff and lucerne chaff as a basal diet will increase microbial protein supply to the intestines. The experiment was conducted in a Latin square design (4 x 4) using four mature merino sheep. The treatments were: T0 = basal diet ad libitum + nil supplements, T1 = T0 + faba beans (FB) fed once daily, T2 = T0 + FB fed twice daily, T3 = T0 + FB fed 8 times daily. The basal diet was given once per day at 09:00 in the morning while FB were given at the rate of approximately 0.5% of live body weight and delivered according to the treatment protocol. Urinary excretion of purine derivative (PD) was used to estimate microbial protein supply. The results indicated that even though treatment statistically had no effects on total urine output, PD excretion in the urine, PD absorbed, estimated microbial N supply, and the efficiency of rumen microbial protein synthesis, provision of supplement to sheep numerically improved microbial N supply by 92% compared to that of control group. However, there were no differences within the supplemented group. Therefore, it is concluded that feeding faba beans more than once a day was unnecessary. Key Words: Purine Derivatives, Microbial Protein Synthesis, Sheep
The Effect of addition different levels of katuk leaf meal in the ration on cholesterol, triglyceride, LDL, and HDL of broiler blood Kamalia, .; Mujnisa, Andi; Natsir, Asmuddin
Buletin Nutrisi dan Makanan Ternak Vol 10, No 1 (2014)
Publisher : Universitas Hasanuddin

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Abstract

The aims experiment were to study the effect of adding different levels of katuk meal on the levels of cholesterol,triglycerides,LDL,and HDL in the blood of broiler chiken. Sixty four (64) treatment diets according to completely randomized design.  The treatment diets were :P0 = contol, P1 = control + 1 % katuk leaf meal, P2 = Control + 2% katuk leaf meal, P3 = control + 3 % katuk leaf meal.  The replication for each treatment was four giving total number of experimental diet was 16.  Control ration consisted of brn, yellow cron, coconut oil, shrimp waste, fish meal, tofu dregs, soy bean.  During the experiment broiler chiken ware given a free access to drinking water.  The resuls of study indicated that addition of katuk leaf meal into the diet did not affect concentration of cholesterol, triglycerides, and LDL in the blood of broiler.  But significantly increased the concentration of blood HDL but had no effects on concenetration of blood cholesterol, blood triglycerides, and blood LDL of broiler.
Preparation of Phosphate Liquid and Urea Molasses Minerals Liquid (UMML) As a Precursor of Rumen Biofermentation Syahrir, Syahriani; Natsir, Asmuddin; Mide, Muhammad Zain; Islamiyati, Rohmiyatul; Asrianie, Ani
Buletin Nutrisi dan Makanan Ternak Vol 9, No 1 (2013)
Publisher : Universitas Hasanuddin

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Abstract

Urea Molasses Minerals Liquid (UMML) may be a precursor of rumen biofermentation. UMML can provide slow-release nitrogen, minerals and readily available carbohydrate (RAC), but require special studies, especially in determining the type and preparation of constituent materials, especially the provision of water-soluble phosphate minerals. The purpose of this study is to produce a formula of UMML then be used as a precursor of rumen biofermentation. Some of the treatment of super phosphate (SP36) was performed to obtain a preparation method produces dissolved  phosphate  concentrations  are high. Preparation  that  the  highest levels of dissolved phosphate is mixed of super phosphate, organic acids and urea solution, then boiled for 5 minutes. Urea Mineral Molasses liqiud mixed from materials Ca(urea)4Cl2 phosphate solution, saturated NaCl solution and molasses.
STUDY ON BUFFALO FATTENING USING AGRICULTURAL WASTE BASED FEEDING AT THE TANA TORAJA DISTRICT Nurhayu, A.; Sariubang, Matheus; Ishak, Andi Baso L.; Natsir, Asmuddin
Proceeding Buffalo International Conference 2013
Publisher : Proceeding Buffalo International Conference

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Abstract

The study was conducted in the Tiromanda village, South Makale sub district, Tana Toraja district. The aim of the study was to measure the effect of providing agricultural waste based feeding to buffalo fattening. Fifteen male buffaloes, aged between 3 - 5 years with body weight ranging between 200 and 250 kg, were randomly assigned to one of three experimental diets, namely (A) 12 kg fermented rice straw + 2.5 kg concentrates; (B) 10 kg fermented rice straw + 5 kg concentrate and (C)control diet (ration typically used by the farmer) according to completely randomised design. Formulation of feed was based on agrricultural waste locally available, i.e. rice straw and concentrates. Determination of body weight was carried out every month for four months. The results showed that the average daily body weight gain (ADG) of buffaloes in the treatment B was the highest compared to that of the treatment A and C, namely 0.8 kg/head/day; 0.6 kg/head /day and 0.3 kg/head/day, respectively. Feed conversion ratio for each treatment was 31.6 (A), 14.87 (B), and 41.3(C). In conclusion, provision of feed based on agricultural waste such as rice straw plus concentrates increased body weight gain in fattening buffaloes.
The effects of microwave radiation on rumen degradation characteristics of barley straw cut at two different stages of maturity Natsir, Asmuddin
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 2 (2007): JUNE 2007
Publisher : Indonesian Animal Sciences Society

Show Abstract | Original Source | Check in Google Scholar | Full PDF (124.149 KB)

Abstract

A common approach for improving the nutritive value of low quality roughages and crop by-products is by pre-treatment or processing either physical, chemical, or biological treatments. Microwave radiation is one type of physical treatment that could be used to treat low quality roughages. Research was carried out to investigate  the effects of microwave radiation on the rumen degradation characteristics of barley straw obtained from two different stages of maturity. The experiment was run factorially based on completely randomized block design. The first factor was stage of maturity, straw cut during the soft elongation time (C1) and during the harvest time (C2). The second factor was levels of microwave radiation times (MWRT) (T0 = control, without MWR; T1 = MWR for 1 minute, T2 = MWR for 2 minutes). The results indicated nutritive values of barley straw obtained from C1 sampling time were significantly better than that obtained from the C2 sampling time in terms of a higher rumen degradation rate and a much greater total potential rumen degradability. In contrast, MWR did not have significant effects on the rate of degradation and total potential degradability of straw in the rumen. Key Words: Microwave Radiation, Barley Straw, Rumen Degradability
Rumen microbial protein supply as estimated from purine derivative excretion on sheep receiving faba beans (vicia faba) as supplement delivered at different feeding frequencies Natsir, Asmuddin
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 2 (2008): JUNE 2008
Publisher : Indonesian Animal Sciences Society

Show Abstract | Original Source | Check in Google Scholar | Full PDF (66.582 KB)

Abstract

Rapid and extensive degradation of faba beans (Vicia faba) by ruminal microbes can result in substantial and undesirable N loss from the rumen. The purpose of this study was to test the hypothesis that offering faba beans as a supplement more than once a day to sheep receiving a combination of oaten chaff and lucerne chaff as a basal diet will increase microbial protein supply to the intestines. The experiment was conducted in a Latin square design (4 x 4) using four mature merino sheep. The treatments were: T0 = basal diet ad libitum + nil supplements, T1 = T0 + faba beans (FB) fed once daily, T2 = T0 + FB fed twice daily, T3 = T0 + FB fed 8 times daily. The basal diet was given once per day at 09:00 in the morning while FB were given at the rate of approximately 0.5% of live body weight and delivered according to the treatment protocol. Urinary excretion of purine derivative (PD) was used to estimate microbial protein supply. The results indicated that even though treatment statistically had no effects on total urine output, PD excretion in the urine, PD absorbed, estimated microbial N supply, and the efficiency of rumen microbial protein synthesis, provision of supplement to sheep numerically improved microbial N supply by 92% compared to that of control group. However, there were no differences within the supplemented group. Therefore, it is concluded that feeding faba beans more than once a day was unnecessary. Key Words: Purine Derivatives, Microbial Protein Synthesis, Sheep
Purine derivatives excretion and estimation of microbial protein supply on sheep receiving different protein suplements Natsir, Asmuddin
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 3 (2007): SEPTEMBER 2007
Publisher : Indonesian Animal Sciences Society

Show Abstract | Original Source | Check in Google Scholar | Full PDF (59.38 KB)

Abstract

The urinary excretion of purine derivative (PD), which consists of allantoin, uric acid, xanthine and hypoxanthine, was used to estimate Microbial Nitrogen (MN) supply on sheep given oaten hay as basal diet supplemented either with faba beans (faba) or barley fortified with urea (barley-urea). Three growing wethers, with average body weight of 43 ± 2.29 kg, were randomly assigned to one of three experimental diets, i.e. oaten hay without supplement (C), diet C + barley-urea (CB), and diet C + faba (CF), according to latin square design (3 x 3). Results of the experiment indicated that PD excretion for C was lower (P = 0.07) than that of CB and CF (2.84 vs 6.08, 7.67 mmol/d). Absorbed PD and estimation of MN supply for C was less (P = 0.07) than those of CB and CF, namely 1.26 vs 6.64, 8.80 mmol/d and 0.91 vs 4.8, 6.30 g N/d respectively. However, the efficiency of MN synthesis was similar, whether the calculation was based on digestible organic matter fermented in the rumen, DOMR (g MN/kg DOMR, P = 0.20) or based on N intake (g MN/g N intake, P = 0.40). In conclusion, supplementation of oaten hay with different protein supplements may improve the estimation of microbial N supply in the growing sheep but both supplements gave similar results on the estimation of MN supply. Key Words: Purine Derivative, Microbial Protein, Protein Supplement, Sheep