Idawati Nasution
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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Persentase Kebuntingan Kambing Betina Resipien Setelah Pelaksanaan Embrio Transfer Riady, Ginta; Adam, Mulyadi; Nasution, Idawati
Jurnal Agripet Vol 2, No 1 (2001): Volume 2, No. 1, April 2001
Publisher : Agricultural Faculty

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ABSTRACT. A study on the pregnanncy rate of recipient does following embryio transfer procedure had been carried out. This study used 2 donor does and 10 recipient does. Donor does are subjected to both estrous synchronization (using progesterone in CIDR-G device) and superovulation protocols using follcle stimulating hormone in Folltropin-V. However, recipient does are only subjected for estrous synchronization protocol. Estrous synchronization protocol ni donor does and recepientb ones are conducted at the same tame. Embryo collection is carried out through cervix at day 7 following breeding. Parameter of this study is pregenancy rate of recipient does fillowing embryo transfer. Data collected in this study will be analyzed descriptively. Recipient does received two embryos by non-surgery method. This study showed that recipient does were foun non-pregnant during pregnancy diagnosis. It can be related to the incidence of early embryonic death are nutrion and overcrowding in uterus. It can be conclude that embryo transfer procedure carried out in this study was not effective.
Spermatogenesis and semen quality of male muntjak (Muntiacus muntjak muntjak) during antler growth periods Wahyuni, Sri; Agungpriyono, Srihadi; Agil, Muhammad; Nasution, Idawati; Hamny, .; Yusuf, Tuty L.
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Muntjak (Muntiacus muntjak muntjak) belongs to Cervidae family which distributed in Java Island and Southern part of Sumatera. This cervid has been protected by Indonesian Government since 1999. In order to support breeding program of the species and to avoid them from extinction, itsreproductive biology such as spermatogenesis and the correlation to semen quality is important to be investigated. Therefore, the objective of this study was to examine spermatogenesis and semen quality of two adult male muntjaks during antler growth periods that consist of hard antler (H), casting (C), andvelvet antler (V). Testicular tissues and semen (ejaculates) were obtained by core needle biopsy and electroejaculation methods respectively. Testicular tissues were processed histologically and stained with periodic acid Schiff (PAS) to observe spermatogenesis whereas semen was evaluated to obtaine itsquality. The results showed that spermatogenic activities were detected in all antler periods which marked by PAS positive staining (magenta colour) of round and elongated spermatid acrosomes. In H period, spermatogenic activity was higher than those C and V periods. According to semen evaluation,motile spermatozoa were found with different concentration in all antler periods. The highest sperm concentration (x 106 spermatozoa/ml) in both of muntjaks was found in H period (506.25 ± 61.87), and slightly decreased in C (288.75 ± 37.12), and V periods (362.60 ± 17.68). These finding showed thatspermatogenesis to produce spermatozoa is taken place while muntjaks are in C and V periods with differ activities that provable with the existence of motile spermatozoa from ejaculates in both of male muntjaks. Therefore, muntjaks could provide reproductive function throughout the year of reproductiveaseasonality which is similar to the reeves and formosan muntjaks
RASIO KETEBALAN DINDING TERHADAP DIAMETER TULANG HUMERUS AYAM KAMPUNG (Gallus domesticus) DAN BURUNG MERPATI (Columba domestica) Nasution, Idawati; RM, Shinta Mutia; -, Hamny
Jurnal Medika Veterinaria Vol 7, No 1 (2013): J. Med. Vet.
Publisher : Universitas Syiah Kuala

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Penelitian ini bertujuan mengetahui perbandingan ketebalan dinding terhadap diameter tulang humerus pada ayam kampung (Gallus domesticus) dan burung merpati (Columba domestica). Dua puluh tulang humerus yang berasal dari 10 ekor burung merpati jantan dipisahkan dari jaringan di sekitarnya, kemudian tulang humerus tersebut direndam dalam formalin 5% dan dipotong secara melintang dengan menggunakan gergaji tulang. Ketebalan dinding terhadap tulang humerus diukur dengan menggunakan jangka sorong. Hasil penelitian dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa perbandingan ketebalan dinding dan diameter tulang humerus pada ayam kampung adalah 1 : 4,94 sedangkan pada burung merpati adalah 1 : 5,80. Tulang humerus ayam kampung lebih tebal dari pada burung merpati jika dibandingkan diameter tulang dari masing-masing unggas tersebut.Kata kunci: Gallus domesticus, Columba domestica, tulang humerus, rasio, diameter, ketebalan
GANGLION SIMPATIS PADA KAMBING LOKAL (Capra sp.) Nasution, Idawati; Yuliansyah, Nanda; -, Hamny
Jurnal Medika Veterinaria Vol 7, No 1 (2013): J. Med. Vet.
Publisher : Universitas Syiah Kuala

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Penelitian ini bertujuan mengetahui struktur anatomi dan letak ganglion simpatis pada kambing lokal (Capra sp.).  Penelitian ini menggunakan empat ekor kambing (dua ekor jantan dan dua ekor betina), rata-rata berumur 2 tahun. Struktur anatomi ganglion dilakukan dengan cara inspeksi dan palpasi setiap ganglion yang diamati. Letak ganglion dideskripsikan berdasarkan letak ganglion terhadap buluh darah atau nervus yang berada di sekitar ganglion. Hasil pengamatan dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa terdapat empat ganglion dari syaraf simpatis pada kambing lokal yaitu ganglion cervicale cranial yang terletak di dorsal arteri occipitalis dan di caudo-medial nervus vagus. Ganglion cervico-thoracicum/stellatum berada di cranial ramus communicantes, di caudal arteri dan vena costocervicovertebralis, di lateral trakea dan di ventral nervus vertebralis. Ganglion celiaco-mesentericum/solare terletak di dorsal arteri mesenterica cranialis dan caudal arteri celiaca. Ganglion mesentericum caudale terletak di antara arteri umbilicalis dextra dan sinistra serta di dorsal arteri sacralis mediana.Dsimpulkan bahwa ganglion celiaco-mesentericum/solare memiliki struktur anatomi yang berbeda dibandingkan dengan ketiga ganglion simpatis lainnya pada kambing lokal
PENGARUH PEMBERIAN PMSG DAN HCG TERHADAP PENINGKATAN JUMLAH KELAHIRAN HAMSTER CAMPBELL (Phodopus campbelli) (Effect of PMSG and hCG to Increase Birth Number in Campbell’s Hamsters (Phodopus campbelli)) Afriani Nur, Novi Afriani Nur; Siregar, Tongku Nizwan; Hamdan, Hamdan; Nasution, Idawati; Thasmi, Cut Nila; Dasrul, Dasrul
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

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The aim of this study was to determine the effect of superovulation induction through Pregnant Mare’s Serum Gonadotropin (PMSG) and Human Chorionic Gonadotropin (hCG) injection to increased birth number in Campbell’s hamsters (Phodopus campbelli). This study used 10 unpregnant female Campbell’s hamster, aged 4-6 months, weighing of 25-30 grams. Hamsters were divided into K1 and K2, each consist of five hamsters. K1 injected with 1 ml physiologic NaCl, while K2 intraperitoneally injected with PMSG and hCG in 5 IU. Mating was held after injection. Male and female hamsters were put in one box with ration of 1:1. The observation of vagina plug was done in order to confirm whether the mating successes or not. The average amount of fetus birth through superovulation on K1 and K2 were 3.40±1.3 and 7.80±1.1, respectively. In conclusion, the superovulation induction using the combination of PMSG and hCG increase birth number on Campbell’s hamsters.
INTERPRETASI UKURAN JANTUNG ORANGUTAN SUMATERA(Pongo abelii) BERDASARKAN FOTO RONTGEN TORAKS DI PUSAT KARANTINA ORANGUTAN SUMATERA UTARA Minati, Qaida; Sayuti, Arman; Nasution, Idawati
Jurnal Medika Veterinaria Vol 7, No 2 (2013): J. Med. Vet.
Publisher : Universitas Syiah Kuala

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Penelitian ini bertujuan mengetahui ukuran jantung orangutan sumatera (Pongo abelii) berdasarkan foto rontgen toraks dengan proyeksi posterior anterior. Foto yang digunakan adalah foto rontgen orangutan sehat sebanyak 48 ekor di Pusat Karantina Orangutan Sumatera Utara yang terdiri atas 24 ekor jantan dan 24 ekor betina yang merupakan sitaan dari Mei 2003 hingga November 2011.  Foto rontgen dikelompokkanberdasarkan jenis kelamin dan tingkatan umur. Pengukuran ukuran jantung dilakukan berdasarkan foto rontgen toraks dan selanjutnya  dilakukanperhitungan dengan cardiothoracic ratio (CTR). Nilai rata-rata CTR pada orangutan jantan dengan kelompok umur  bayi (0-2,5 tahun), anak(2,5-7 tahun), remaja (7-10 tahun) masing-masing adalah 45,2;  41,6; dan 38,8% sedangkan untuk orangutan betina masing-masing adalah 45,6;42,1; dan 40,3%.  Bayi orangutan memiliki nilai CTR yang paling besar.  Cardiothoracic ratio (CTR) menurun pada setiap peningkatan umurdari kedua jenis kelamin
GAMBARAN HISTOLOGI KELENJAR INTESTINAL PADA DUODENUM AYAM KAMPUNG (Gallus domesticus), MERPATI (Columba domesticus) DAN BEBEK (Anser anser domesticus)(Histological Feature of Intestinal Glands of Native Chicken (Gallus domesticus), Pigeon (Columba domesticus), and Duck (Anser anser domesticus)) Zainuddin, Zainuddin; Masyita, Dian; Sarayulis, Sarayulis; Jalaluddin, M.; Rahmi, Erdiansyah; Nasution, Idawati
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

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The aim of this research was to observe intestinal glands histology of duodenum from native chicken, pigeon, and duck. Samples used are duodenum from 3 native chickens, 3 pigeons, and 3 ducks with undifferentiated of sex and weight. Fowl was slaughtered, duodenum were taken out then proceeded for histology method using parraffination and stained with hematoxylin-eosin. Histological observation include the density and morphology of intestinal glands. Data were analyzed descriptively. The results showed that the intestinal glands histology differ among native chicken, pigeon, and duck but the morphology of intestinal gland was similar that was simple tubular. The number of intestinal glands in native chicken and pigeon almost similar and found in high density but lower than that found in pigeon. This difference assumed to be correlated with type and concentration of feed.
EFEK EKSTRAK DAUN PEGAGAN (Centella asiatica (L.) Urban) TERHADAP PERKEMBANGAN SEL SPERMATID TIKUS (Rattus norvegicus) Sihombing, Wahyu; Akmal, Muslim; Wahyuni, Sri; Nasution, Idawati; -, Rinidar; -, Hamdan
Jurnal Medika Veterinaria Vol 9, No 1 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

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Penelitian ini bertujuan mengetahui efek pemberian ekstrak daun pegagan terhadap perkembangan sel spermatid tikus. Penelitian ini merupakan penelitian eksperimental dengan menggunakan rancangan acak lengkap (RAL) pola one way analysis of varian (ANOVA). Tikus yang digunakan adalah tikus jantan berumur 3,5 bulan dengan bobot badan 150-250 g sebanyak 12 ekor tikus. Tikus-tikus tersebut dikelompokkan menjadi empat kelompok perlakuan dengan masing-masing tiga ulangan. Kelompok pertama merupakan kelompok kontrol (K0) yang tidak diberi perlakuan, sedangkan kelompok K1, K2, dan K3 diberi perlakuan berupa ekstrak daun pegagan dengan dosis bertingkat, yaitu 125, 250, dan 500 mg/kg bobot badan selama 30 hari. Pada akhir perlakuan, tikus dikorbankan dengan diberi eter, lalu testis dinekropsi untuk selanjutnya dibuat preparat histologis.Preparat histologis diwarnai dengan pewarnaan periodic acid Schiff (PAS) untuk mengetahui perkembangan sel spermatid dengan cara mendeteksi karbohidrat netral pada tudung akrosom sel spermatid tersebut. Data dianalisis dengan ANOVA dan dilanjutkan dengan uji Tukey HSD. Hasil penelitian memperlihatkan bahwa rataan jumlah sel spermatid mengalami penurunan dibandingkan kontrol (K0). K0 mempunyai rataan jumlah sel spermatid tertinggi yaitu 308,00±56,33; diikuti oleh K1 yaitu 234,38±19,81; K2 yaitu 218,50±5,48; dan K3 yaitu 208,05±27,35. Hasil uji statistikmenunjukkan adanya pengaruh yang signifikan (P<0,05) antara dosis dan rataan jumlah sel spermatid antara K0 dengan K2 dan K0 dengan K3. Kesimpulan dari penelitian ini adalah ekstrak daun pegagan dapat mengurangi laju perkembangan sel spermatid tikus.
PREVALENSI PERUBAHAN PERILAKU ANJING LOKAL (Canis familiaris) JANTAN YANG DIKANDANGKAN DENGAN PRINSIP KESEJAHTERAAN HEWAN SELAMA 60 HARI (Prevalence of Behavioural Changes in Male Local Dog (Canis familiaris) Caged Based on Animal Welfare Principles for 60 Days) Alfi, Nofri; Ferasyi, Teuku Reza; Rahmi, Erdiansyah; Adam, Mulyadi; Nasution, Idawati; Ismail, Ismail
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

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The aim of this research was to determine the prevalence of behavioral changes of male local dog caged based on animal welfare principles. A total of three male local dogs was used as the experiment object. Dog behavior was observed using focal animal sampling method. Dogs were caged for 60 days, provided with sufficient food and water ad libitum. The dogs were identified based on their color and body size. Their behavior were observed three times each days in the morning, midday, and afternoon with 3 periods: period I (day 1 to day 20), period II (day 21 to day 40), and period III (day 41 to day 60). The observation was carried out for 60 days with allocation time was 30 minutes/ each observations. The result obtained indicated that the prevalence of behavior in dogs during the first period was dominated by sitting and sleeping. In addition, they were showed the behavior of running, walking, playing, sniffing, and eating. During this period, they were also expressed of stress behavior, such as barking, digging, backward/hiding, biting, and climbing. In the second and third periods of observation, the behaviors of sitting and sleeping are increased while behaviors of stress are reduced. Based on this results, it can be concluded that local dogs indicate stress behavior during the first 20 days caged, then they become more calm during the following days when animal welfare principle is fulfilled.Key words: local dog, dog behavior, animal welfare
TINJAUAN ASPEK KESEJAHTERAAN HEWAN PADA SAPI YANG DIPOTONG DI RUMAH PEMOTONGAN HEWAN KOTAMADYA BANDA ACEH (Study of the Animal Welfare Aspect on Cattle Slaughtered in Slaughter house in Banda Aceh) Bhaskara, Yudha; Adam, Mulyadi; Nasution, Idawati; Lubis, Triva Murtina; Armansyah, Teuku; Hasan, Muhammad
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

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This study was conducted to obtain the information about animal welfare on cattle slaughtered in Slaughterhouse in Banda Aceh. The animal welfare parameter was observed related to the transport aspect, shelter aspect, and slaughter aspect. The three aspects compared to the recommendation of Meat Livestock Australia (MLA) and Indonesian National Standard 02-4509-1998. Method used to determine the animal welfare was scoring evaluation. The result of study showed that transport aspect get 20 score with a percentage of 50% which categorized as sufficient. Shelter aspect get 33 score with percentage of 82,5% which categorized as good, and slaughter aspect get 32 score with percentage of 80% which categorized as good. Based on scoring evaluation of shelter aspect and slaughter aspect, the animal welfare of cattle slaughtered in Slaughter house in Banda Aceh is considered good, meanwhile the transport aspect is considered sufficient.Key words: animal welfare, cattle, slaughterhouse