Nasronudin Nasronudin
Tropical Disease Diagnostic Center (TDDC) – Institute of Tropical Disease, Universitas Airlangga.

Published : 44 Documents
Articles

Phylogenetic Molecular Analysis Human Immunodeficiency Virus (HIV) Patients in Surabaya, East Java Nasronudin, Nasronudin; Lusida, Maria Inge; Handajani, Retno; Lindawati, Lindawati; Efendi, Ferry; Utsumi, Takako
Journal of the Indonesian Medical Association Vol. 60 No. 4 April 2010
Publisher : Journal of the Indonesian Medical Association

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Abstract

The Human Immunodeficiency Virus Type 1 (HIV-1) isolates are classified in three main groups: group M (main), group O (outlier) as well as group N (non-M/non-O). The HIV-1 M group, responsible for the majority of infections in the HIV-1 worldwide epidemic, can be further subdivided into 10 recognized phylogenetic subtypes or clades, A–D and F-K. HIV-1 phylogenetic classifications are currently based on nucleotide sequences derived from such as gag p17 region of the same isolates or on full-length genome sequence analysis. We do not know HIV subtype distribution in HIV suspected patients, in Surabaya, East Java. The aims of this study was to do molecular analysis HIV in patients with HIV infection, in Surabaya, East Java. Antibody to HIV were detected using 3 methods, paper and EIA (Acon) and ELISA (Axion) techniques from 51 plasma obtained from the patients suspected HIV infection, in Surabaya, Indonesia All of the samples were subjected to Polymerase Chain Reaction (PCR) using pairs of primers based on HIV gag p17 genes. The PCR positive samples were sequenced and analysed to identify the HIV subtype using Genetic Version 9 program. Fourty nine (96.08%) HIV antibody were detected from 51 patients suspected HIV infection and 57.14% (28/49) HIV RNA determination positives. All of 21 positives HIV DNA except one sample that have been analyzed was CRFs of HIV with mayority CRF01-AE subtype similar with HIV CRF01-AE subtype in Asia countries, e.g. Thailand, Japan, Malaysia, Cina and Hongkong. Those one sample has 18 nucleotides insertion look like a HIV new subtype but it is needed to confirm further. From gag p7 HIV gene in this study, one HIV has and CRF01-AE is majority HIV subtype in Surabaya, East Java which is located in the same branch with HIV common CRF01-AEHIV subtype in Asia.Keywords: HIV subtype, gag p17 gene, Surabaya, Indonesia
COLLOID AND METHYLPREDNISOLONE THERAPY AS ALTERNATIVE MANAGEMENT OF DHF Nasronudin, Nasronudin; P, Widiyanti; MV, Arfijanto; M, Rusli
Buletin Penelitian Sistem Kesehatan Vol 13, No 3 Jul (2010)
Publisher : Buletin Penelitian Sistem Kesehatan

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Abstract

There are three phase in progresivity of dengue infection which are afferent phase, efferent phase and efector phase. In dengue infection, it has been found endothelial cells leak age, the pro inflammatory cytokine level alteration and other mediator followed by the plasma migration and it has the potency to become dengue shock. The mam clinical manifestationare fever and bleeding. The aim of this research is to prove the influence of colloid and methylpredmsolone theraphy to inhabit the endothelial cells leakage through the alteration of IL-1b. TNF-a and PLA-2 level. This research has been involved 36 dengue patients in Tropical Infection Division, Departement of lntemal Medicine Medical Faculty Airlangga University -Dr. Soetomo Hospital Surabaya and 36 control group. We did the examination of cytokine level (IL-1b. TNF-a PLA-2) on blood sample with ELISA method. The dose methylprednisolone 125 mg twice a day and colloid (MW 40 kDa) 500 cc perday for 3 days has been given to the patient whose fulfilled the inclusion critena. We found temperature change to normal level after intervention. In conclusion, colloid and methylprednisolone therapy is effective to accelerade the temperature, IL -1b, TNF-a, and PLA-2 level declination, accelerate the endothelial cells sealing and plasma migration in dengue infective patient. Key words: DHF, Colloid, Methylprednisolone, proinflammatory cytokine
HIV Genotype Analysis from HIV Infected Patients in East Java Area Ismail, Yulia Sari; Soetjipto, Soetjipto; Wasito, Eddy Bagus; Nasronudin, Nasronudin
Jurnal Natural Volume 12, Number 2, September 2012
Publisher : Syiah Kuala University

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Abstract

Human Immunodeficiency Virus type 1 (HIV-1) has been known to cause Acquired Immune Deficiency Syndrome (AIDS) disease and has been alaso divided into several subtypes (A, B, C, D, F, G, H, J, K) and Circulating Recombinant Form (CRF). Different characteristics of subtype of the virus and its interaction with host can affect the severity of the disease. This study was aimed to analyze HIV-1 genotypes circulating in HIV/AIDS patients from the East Java region descriptively. Information from this research was expected to complement the data of mocular epidemiology of HIV in Indonesia.  This study used blood plasma from patients who had been tested to be HIV positive who were seeking treatment or are reffered to the Intermediate Care Unit of Infectious Disease (UPIPI) Dr. Soetomo Hospital Surabaya from various area representing the East Java regions. Plasma was separated from blood samples by centrifugation for use in the the molecular biology examination including RNA extraction, nested PCR using specific primer for HIV gp120 env gene region, DNA purifying, DNA sequencing, and homology and phylogenetic analysis. Based on the nucleotide sequence of the HIV gp120 env gene, it was found that the most dominant genotypes in East Java belonged to one group of Circulating Recombinant Form (CRF), namely CRF01_AE and CRF3x_01B, which has been also found in Southeast Asia. In the phylogenetic tree, most of HIV samples (30 samples) were in the same branch with CRF01_AE and CRF3x_01B, except one sample (HIV40) was in the same branch with subtype B.
ANALYSIS OF HIV SUBTYPES AND CLINICAL STAGING OF HIV DISEASE/AIDS IN EAST JAVA Ismail, Yulia; Soetjipto, Soetjipto; Wasito, Eddy; Nasronudin, Nasronudin
Indonesian Journal of Tropical and Infectious Disease Vol 3, No 2 (2012)
Publisher : Institute of Topical Disease

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Abstract

Human Immunodeficiency Virus type 1 (HIV-1) known to cause Acquired Immune Deficiency Syndrome (AIDS) disease are divided into several subtypes (A, B, C, D, F, G, H, J, K) and Circulating Recombinant Form (CRF). Different characteristics of subtype of the virus and its interaction with the host can affect the severity of the disease. This study was to analyze HIV-1 subtypes circulatingin HIV/AIDS patients from the East Java region descriptively and to analyze its relationship with clinical stadiums of HIV/AIDS. Information from this research was expected to complement the data of mocular epidemiology of HIV in Indonesia. This study utilited blood plasma from patients who had been tested to be HIV positive who sected treatment to or were reffered to the Intermediate Care Unit of Infectious Disease (UPIPI) Dr. Soetomo Hospital Surabaya from various area representing the East Java regions. Plasma was separated from blood samples by centrifugation for use in the the molecular biology examination including RNA extraction, nested PCR using specific primer for HIV gp120 env gene region, DNA purifying, DNA sequencing, and homology and phylogenetic analysis. Based on the nucleotide sequence of the HIV gp120 env gene, it was found that the most dominant subtypes in East Java were in one group of Circulating Recombinant Form (CRF) that is CRF01_AE, CRF33_01B and CRF34_01B which was also found in Southeast Asia. In the phylogenetic tree, most of HIV samples (30 samples) are in the same branch with CRF01_AE, CRF33_01B and CRF34_01B, except for one sample (HIV40) which is in the same branch with subtype B. HIV subtypes are associated with clinical stadiums (disease severity) since samples from different stages of HIV disease have the same subtype.
BACTERIA CAUSED SEPSIS BIOMARKERS Tjempakasari, Artaria; Nasronudin, Nasronudin
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 3 (2014)
Publisher : Institute of Topical Disease

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Abstract

Sepsis is a clinical condition of patients with serious infections that show a systemic inflammatory response, with or without a positive blood culture. sepsis is one of the most frequent causes of death in patients in intensive care units. We are at urgent need for biomarkers and reliable measurements that can be applied to risk stratification of septic patients and that would easily identify those patients at the highest risk of a poor outcome. Such markers would be of fundamental importance to decision making for early intervention therapy. Pro-inflammatory cytokines such as tumor necrosis factor- (TNF- ), interleukins-1,-6,-8 (IL-1, IL-6, IL-8) are postulated to play a major role in the pathogenesis of the syndrome. C-reactive protein (CRP) and procalcitonin (PCT) are among a few biomarkers thatincorporated into clinical practice although their precise role in the pathopysiology of sepsis and organ dysfunction still unclear.
The Prevalence of Human Immunodefiency Virus-1 (HIV-1) Subtypes and Transmission Method among HIV/AIDS Infection Patient in Tulungagung, East Java Indonesia Ardianto, Achmad; Khairunisa, Siti Qamariyah; Kotaki, Tomohiro; Witaningrum, Adiana Mutamsari; Qushay, M.; Juniastuti, Juniastuti; Rahayu, Retno Pudji; Widiyanti, Prihartini; Utomo, Budi; Lusida, Maria Inge; Nasronudin, Nasronudin
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 5 (2015)
Publisher : Institute of Topical Disease

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The rapid epidemic growth of HIV is continuing in Indonesia. There are some factors which have influenced the spreading of this epidemic in Indonesia, such as the poor awareness to avoid unsafe free sex attitude and the sharing of needles and syringes among intravenous drug users (IDUs). The sexual transmission of HIV has also apparently increased in Tulungagung. Commercial sex workers play a significant role in the spread of HIV in Tulungagung. People in Tulungagung have worked at other countries as Indonesian migrants. This condition can cause the increase number of HIV-1 case and the possibility of genetic variation (subtype) HIV-1 in Tulungagung. This research is aimed to analyze the subtype and to determine estimation of transmission mode on infected patient of HIV-1 and AIDS who came to Seruni clinic Dr. Iskak hospital in Tulungagung. 40 HIV?AIDSpatients were interviewed to determine the subtype and the transmission mode. The results showed that 14 of 40 plasma samples (35%) were successfully to amplified and sequenced. OverallCRF01-AE wereidentified as predominant subtype among HIV/AIDS patients in Tulungagung. Based on individual information, 31 of 40 subjects (77%) were heterosexual transmission.
PATHOGENESI S, DI AGNOSTI C AND MANAGEMENT OF TOXOPLASMOSIS Yuliawati, Irma; Nasronudin, Nasronudin
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 4 (2014)
Publisher : Institute of Topical Disease

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Abstract

Toxoplasma gondii is an obligate intracellular parasite of protozoa groups, can infect humans and all warm-blooded animals, are found in almost all locations around the world. Infection generally occurs orally through the consumption of animal products that are not perfectly cooked infected oocyst, parasite containing foods in the form of bradyzoite, contact with cat’s feces containing oocysts or vertical transmission occurring through hematogenous placenta. Toxoplamosis can occur in acute or chronic. It divided into five categories, namely, toxoplasmosis in patients immunocompetent, toxoplasmosis in pregnancy, congenital toxoplasmosis, toxoplasmosis in immunocompromised patients and ocular toxoplasmosis. In each category of clinical manifestations of toxoplasmosis are often non-specific. Methods of diagnosis and interpretation are often different for each category. Toxoplasmosis can be diagnosed through aseries of tests such as serology, PCR, histology parasites and parasite isolation. Treatment management of this disease requires a long time. Therapy depends on the category of infections as well as individual therapeutic response. The combination of pyrimethamine with sulfadiazine is the drug choice for toxoplasmosis.
A PATIENT DENGUE HEMORRHAGIC FEVER WITH SPASMS Kholili, Ulfa; Nasronudin, Nasronudin
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 3 (2014)
Publisher : Institute of Topical Disease

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Abstract

Indonesia is one of the countries with the high endemic of Dengue viral infection followed by Thailand, Myanmar, India and Srilanka. For more 10-15 years, Dengue Viral Infection/DHF has become a cause of patient who should be hospitalized and was the first cause of death children in south easthern Asia.1,2 Batavia was the first city of Indonesia found Dengue Viral infection which had been written in journal by David Bylon in the 1779. Encephalopathy of dengue (ED) is one unusually complication of dengue viral infection which had been characterized by aberration the arrangement of nerves central (CNS). This paper want to describe of a young teenage with suffer from DHF and seizure. Beside it, pleural effusion and cerebral edema had been found. Seizure most likely due to dengue encephalopathy associated with cerebral edema and was supported by positive IgG and IgM anti dengue. Corticosteroid was given toimprove cerebral edema. By good management as long as admission, she was discharged from hospital with a good condition.
Management Patient of Swine Influenza Gunawan, Endra; Nasronudin, Nasronudin
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 5 (2015)
Publisher : Institute of Topical Disease

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Influenza is an acute respiratory diseases caused by various influenza virus which infect the upper and lower respiratory tract and often accompanied by systemic symptoms such as fever, headache and muscle pain. Influenza spreads through the air. Swine influenza comes from swine and can cause an outbreaks in pig flocks. Even this is a kind of a rare case but the swine influenza could betransmitted to human by direct contact with infected swine or through environment that already being contaminated by swine influenza virus. There are 3 types of swine influenza virus namely H1N1, H3N2 and H1N2. Type H1N1 swine-virus had been known since 1918. Avian influenza virus infection is transmitted from one person to another through secret containing virus. Virus is binded into the mucous cells of respiratory tract before it is finally infecting the cells itself. Management patients with H1N1 influenza is based on the complications and the risk. Besides, it is also need to consider the clinical criteria of the patient. Therapy medicamentosa is applied to the patients by giving an antiviral, antibiotics and symptomatic therapy. Prevention can be done by avoid contact with infected animal or environment, having antiviral prophylaxis and vaccination.
HIV and Malaria Parwitasari, Ririek; Nasronudin, Nasronudin
Indonesian Journal of Tropical and Infectious Disease Vol 5, No 1 (2014)
Publisher : Institute of Topical Disease

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Abstract

HIV/AIDS is a global problem involving industrialized and developing country including Indonesia. Malaria has killed millions of human beings almost 3 million people each year, whereas since 1999, nearly 36 million people in the world infected with HIV and 3 million more have died (Kakilaya, 2006). HIV infection increases the risk and aggravate malaria. In Africa in the area of malaria transmission intensities high and low, HIV aggravate malaria and improve case fatality at any age (Eline 2006). HIV is an RNA viruses whose hallmark is the reverse transcriptation of its genomic. Malaria is a protozoan disease transmitted by the bite of infected anopheles mosquito. Infection malaria can stimulate HIV replication and may cause faster progression of HIV disease.