Taufiqurrachman Nasihun
Department of Biochemistry and Andrology, Faculty of Medicine, Sultan Agung Islamic University, Semarang
Articles
12
Documents
Pengaruh Pemberian Ekstrak Purwoceng (Pimpinella alpina Molk) terhadap Peningkatan Indikator Vitalitas Pria Studi Eksperimental pada Tikus Jantan Sprague Dawley

Jurnal Sains Medika Vol 1, No 1 (2009)
Publisher : Fakultas Kedokteran UNISSULA

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Abstract

Background. Pimpinella alpina Molk (PAM) or “purwoceng” has been traditionally used as an alternativetreatment to enhance male vitality. Unfortunatelly, the effect of PAM to enhance vitallity which is indicated byincreasing in consentration of Te, LH, and FSH has not been fully understood. This study was conducted toelucidate whether treatment of PAM crude extract will be able to enhance vitality which is indicated withincreasing in level of testosteron, LH, and FSH.Design and Method: In the Post Test Control Group Design, 30 male of three month-old Sprague Dawley ratsrandomly assigned into three groups, i.e Group A as a control, Group B, and group C was treated orally withaquadest 2 ml, 1 ml (25 mg), and 2 ml (50 mg) of PAM crude extract respectively for seven days. tetestosteron,LH, and FSH level were assessed by RIA and IRMA respectively. The different level of testosteron, LH, and FSHamong the three groups was analyzed by ANOVA, followed by Post Hoct Tukey HSD.Result: The result showed significantly higher serum testosterone and LH level (p=0.049 and p=0.853respectively) in 50 mg treated rats when compared with control while no significant difference was seen in theserum levels of these two markers between 25 mg treated rats and 50 mg treated rats. Statistical comparisonrevealed no significant difference in FSH level either in the two treated groups as compared to control or 25mgtreated group to 25 mg treated group.Conclusion: Treatment of extract PAM 50 mg could enhance vitality which is indicated with increasing intestosterone and LH level, but had no effect to FSH level, (Sains Medika, 1 (1) : 53-62).Pendahuluan: Pimpinella alpina Molk (PAM) atau purwoceng telah digunakan sebagai obat alternatif secaraturun temurun untuk meningkatkan vitalitas pria. Namun, pengaruh ekstrak PAM terhadap peningkatan vitalitasdengan indikator peningkatan kadar Te, LH, dan FSH masih belum jelas. Penelitian ini bertujuan untukmengetahui apakah pemberian ekstrak PAM mampu meningkatkan vitalitas dengan indikator peningkatankadar testosteron, LH, dan FSH.Metode Peneletian: Post Test Control Group Design dengan sampel 30 tikus jantan Sprague Dawley umur 3bulan dibagi menjadi 3 group secara random masing-masing terdiri dari 10 ekor tikus. Group A, sebagai kontroldiberi aquadest 2 ml, Group B dan Group C masing-masing diberi 1 ml ekstrak PAM (25 mg) dan 2 ml ekstrakPAM (50 mg). Setelah satu minggu aklimatisasi tikus diberi perlakuan selama 7 hari berturut-turut denganacufirm (blunt type needle). Te kemudian diperiksa dengan metode Radio Immuno Assay (RIA), sehingga Teyang diukur adalah Te total (TTe), sedangkan LH dan FSH diperiksa dengan metode Immuno Radiometric Assay(IRMA).Kadar testosteron, LH, dan FSH, masing-masing diuji dengan metode RIA dan IRMA. Perbedaan kadartestosteron, LH, dan FSH di antara group dianalisis dengan ANOVA dan dilanjutkan dengan uji Post Hoct TukeyHSD.Hasil Penelitian: Hasil penelitian menunjukkan bahwa group C mempunyai rerata kadar testosteron lebihtinggi bermakna dibanding group A, namun tidak bermakna dibanding group B. Rerata kadar testosterongroup B lebih tinggi dibanding group A namun tidak bermakna. Rerata kadar LH group C lebih tinggi bermakna dibanding group A, namun tidak bermakna dibanding group B. Rerata kadar LH group B lebih tinggi dibandinggroup A namun tidak bermakna. Rerata kadar FSH group C lebih tinggi dibanding group A dan group B namuntidak bermakna, demikian pula dengan group B dibanding group A.Kesimpulan: Pemberian ekstrak PAM 50 mg mampu meningkatkan vitalitas dengan indikator peningkatankadar Testosteron dan LH, namun tidak meningkatkan kadar FSH, (Sains Medika, 1 (1) : 53-62).

The Effect of Moringa Oleifera. Lam Leaf Extract on Bcl2 and Bax Expression in Paracetamol-induced Renal Tubular Apoptosis in Rats

Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 8, No 2 (2017): July-Desember 2017
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

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Abstract

BACKGROUND: Paracetamol may cause apoptosis in the renal tubules. Moringa leaf (Moringa oleifera. Lam) has been shown to have antioxidanteffects due to its flavonoids. In addition, flavonoids also have biological effects to modulate the signal-transduction pathway. OBJECTIVE: to investigatethe effect of Moringa oleifera Lam extract on Bcl-2 and Bax expression on paracetamol-induced renal tubular injury.METHODS: in this post-test only control group design study, 24 rats were divided into 4 groups: AqC-G (aquades), EtC-G (70% ethanol), MoL1-Gand MoL2-G (moringa leaf extract at the dose of 1.075 g/Kg BW and 2.150 g/Kg BW respectively). Expression of Bcl2 and Bax was determined usingimmunohistochemical staining. The data on the number of Bcl2 and Bax expressions tested with One Way Anova followed by the Post Hoc LSD test.RESULTS: Post Hoc LSD test showed that the expression of Bcl-2 in the experimental groups ((MoL1-G (9.1) and MoL2-G (13,683)) was significantlyhigher compared to that of controls (p<0.001). Bax expression in the experimental groups (MoL1-G (6,85) and MoL2-G (2,633)) was significantly lowercompared to that of controls, p <0.001.CONCLUSION: The administration of Moringa leaf extract at the dose of 1,075 and 2,150 increases Bcl-2 expression and decreases Bax expression inparacetamol-induced renal tubular injury.

Increase in Bcl2 expression of penile and prostate cells of Sprague Dawley male rats following treatment with buceng (combination of Pimpinella alpina molk with Eurycoma longifolia Jack)

Medical Journal of Indonesia Vol 24, No 1 (2015): March
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

Background: Treatment with buceng combination of Eurycoma longifolia Jack and Pimpinella alpine Molk has been proven to increase testosterone level, decrease apoptosis and caspase3 expression. Bcl2 is an antiapoptotic protein found in cytoplasm which inhibits cells apoptosis. This study was aimed to investigate the effect of buceng on Bcl2 expression on penile and prostate tissues of the rats. Methods: In this experimental study, 24 male Sprague Dawley rats of 90 days old, weighing ± 300 grams, were randomly assigned into four groups. Group A, normal rats. Group B, castrated rats and treated with buceng 100 mg/day, per oral (Cast-Bcg); Group C, castrated rats and treated with 2 ml of water as placebo against buceng (Cast-Plac). Group D, castrated rats, treated with mesterolone 6.75 mg/day, per oral, as exogenous testosterone (Cast-Mest). All rats were treated for 30 days. Manova test was used to analyze the different expression of Bcl2 among groups with significance level at p ≤ 0.05. Results: Castration was associated with significant decrease of Bcl2 expression in the penile and prostate tissues (53.0 and 50.9%, respectively) compared to normal rats (82.6 and 84.2%, respectively, p < 0.001). Treatment with mesterolone reversed Bcl2 expression (77.1 and 78.1%) to a near normal level. The same level of Bcl2 expression was also observed with buceng treatment (73.8 and 78.2%).Conclusion: The treatment with buceng could enhance Bcl2 expression in penile and prostate tissues, comparable to normal rats and mesterolone treated rats.

Editorial

Sains Medika Vol 8, No 1 (2017): January - June 2017
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

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Abstract

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Stem Cell Mesenchymal Injection Increases Platelet-Derived Growth Factors Level and Percentage of Collagen in Third-Degree Burn injured Mice

Sains Medika Vol 7, No 2 (2016): July-December 2016
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

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Abstract

Introduction: Mesenchymal stem cell (MSC) has been demonstrated to accelerate wound healing. Platelet-derived growth factor (PDGF) and collagen have an important role in wound healing. However, the effect of MSC on levels of PDGF and number of collagen has not been established. Objectives: To determine the effect of MSC on the levels of PDGF and the percentage of collagen in the burns healing. Methods: in this experimental study, 20 BALB /c mice were divided into 4 groups: a control group (G-0) injected with 2 ml of distilled water and group 1 (G-1), Group 2 (G-2), and group 3 (G- 3) injected with MSC at the dose of 1 x 104, 2 x 104, and 4 x 104 respectively. The burn wound was made by attaching a metal nail that had been heated for 20 seconds at the feet of mice. Levels of PDGF on day 2 (PDGF-2) and 7 (PDGF-7) was assessed by ELISA, while on day 10 excision of skin was done to calculate the percentage of collagen. Results: There was a significant difference in the levels of PDGF-2, PDGF-7, and the percentage of collagen ( P <0.05). Posthoc analysis showed that the levels of PDGF-2, PDGF-7, and percentage of collagen in the G-2 and G-3 was significantly higher than that of G-0, p <0.05. While the levels of PDGF-2, PDGF-7, and the percentage of collagen in the G-3 was significantly higher than that of G-2 and G-1 (p <0.05). Conclusion: Injection of MSC at a dose of 2 x 1044 and 4 x 1044 increases the levels of PDGF and the percentage of collagen.

Calorie Restriction, Stem Cells, and Rejuvenation Approach

Sains Medika Vol 7, No 2 (2016): July-December 2016
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

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Abstract

Aging may be defined as the time-dependent deterioration in function of an organism associated with or responsible for the increase in susceptibility to disease and probability of death with advancing age (Harman, 1981; Cefalu, 2011). Generally, the aging organisms are characterized by both biochemical and functional declines. Declining of basal metabolism rates, protein turnover, glucose tolerance, reproductive capacity, telomere shortening, and oxidative phosphorylation are related to the biochemical. Whilst, lung expansion volume, renal glomerular and tubular capacities, cardiovascular performance, musculoskeletal system, nerve conduction velocity, endocrine and exocrine systems, immunological defenses, and sensory systems are associated with the physiological declining (Baynes and Dominiczak, 2015). Some evidences indicated that, although members of a species develop into adults in the same way, even genetically similar or identical individuals, raised in identical conditions and eating identical food, but they may age differently (Baynes and Dominiczak, 2015). These aging differences are attributable to the life style particularly calorie and dietary restriction intakes, reactive oxygen species (ROS) production, and thus its implication on severity of damage, repair capacity, and error accumulation in cellular genetic material (Baynes and Dominiczak, 2015; Mihaylova et al., 2014; Mazzoccoli et al., 2014). Therefore, in molecular terms, aging can be defined as a decline of the homeostatic mechanisms that ensure the function of cells, tissues, and organs systems (Mazzoccoli et al., 2014). Accordingly, if the homeostatic mechanism can be repaired, the result is rejuvenation.

Flavonoids and Aging

Sains Medika Vol 7, No 1 (2016): January - June 2016
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

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Abstract

Aging is inevitable process, however human comes into aging is in difference way and difference rates. Some of them undergo aging earlier due to degenerative diseases, unable to reach the maximum lifespan and are thus denominated secondary aging (Huebschmann AG, 2011). Some of others undergo aging latter, looked younger than their truely chronological age, have no suffer degenerative disease, able to achieve maximum lifespan and so called primary aging (De la Fuente, 2009; Huebschmann AG, 2011). Maximum lifespan or often called longevity of the species is defined as maximum time that a species belonging to determine how long the species can live, for example maximum lifespan in human beings is 122 years, whereas the lifespan in rats and mouse strains is only 3 and 4 years respectively (De lafuente, 2009). It must be distinguished this longevity from mean or averages longevity. Average longevity can be defined as the average of time that member of population that have been born on the same date live (De la Fuente, 2009). The maximum lifespan is fixed in each species, meanwhile the lifespan of individual subject in the population, even when their genotype are the same and grow in a common environment condition, such as protected from external hazard show marked variability (Kirkwood, 2005).

The Administration of Orange Sweet Potato (Ipomoea Batatas L.) Extract Increases Sperm Consentration, Mortility and Viability in Male Mice Exposed to Cigarette Smoke

Sains Medika Vol 8, No 1 (2017): January - June 2017
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

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Abstract

Pendahuluan: Ipomoea batatas L (IPL) mengandung antioksidan betakaroten, vitamin C, dan vitamin E. Rokok mengandung ROS yang terbukti menurunkan kualitas spermatozoa. Tujuan: untuk membuktikan bahwa pemberian IPL dapat memperbaiki jumlah, motilitas, dan viabilitas spermatozoa yang dipapar asap rokok. Metode: Penelitian eksperimental dengan post test only control group design, sebanyak 30 ekor mencit jantan, dibagi menjadi 6 kelompok. Kelompok normal (Nor-G), tidak ada interfensi; Kelompok kontrol negative (Neg-G), hanya dipapar asap rokok; Kelompok kontrol positif (Pos-G), hanya mendapat ekstrak IPL 16 mg/ml; Kelompok IPL-15, IPL-16, dan IPL-17, masing-masing dipapar asap rokok dan mendapat ekstrak IPL 15 mg/ml, 16 mg/ml dan 17 mg/ml.  Hasil: hasil uji Post Hoc LSD menunjukkan bahwa jumlah sperma pada IPL-15, IPL-16, dan IPL-17, lebih tinggi bermakna dibanding Neg-G, p < 0.05 dan lebih rendah dibanding Nor-G dan Pos-G, p < 0.05. Demikian pula dengan persentase viabilitas sperma. Hasil uji Mann Whitney menunjukkan bahwa persentase motilitas sperma pada IPL-15, IPL-16, dan IPL-17, lebih tinggi bermakna dibanding Neg-G, p < 0.05. Sedangkan dibanding Nor-G dan Pos-G persentase motilitas sperma pada IPL-15, IPL-16, dan IPL-17, tidak berbeda bermakna, p > 0.05. Kesimpulan:  pemberian ekstrak ubi jalar jingga dapat meningkatkan jumlah, motilitas, dan viabilitas spermatozoa mencit jantan yang dipapar asap rokok.                       

Successful Aging

Sains Medika Vol 6, No 1 (2015)
Publisher : Fakultas Kedokteran UNISSULA

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Abstract

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Ramadan Fasting, Health, and Autophagy: Is There any Relationship?

Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 8, No 2 (2017): July-Desember 2017
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

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Abstract

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