Taufiqurrachman Nasihun
Department of Biochemistry and Andrology, Faculty of Medicine, Sultan Agung Islamic University, Semarang

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Pengaruh Pemberian Ekstrak Purwoceng (Pimpinella alpina Molk) terhadap Peningkatan Indikator Vitalitas Pria Studi Eksperimental pada Tikus Jantan Sprague Dawley Nasihun, Taufiqurrachman
Jurnal Sains Medika Vol 1, No 1 (2009)
Publisher : Fakultas Kedokteran UNISSULA

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Abstract

Background. Pimpinella alpina Molk (PAM) or “purwoceng” has been traditionally used as an alternativetreatment to enhance male vitality. Unfortunatelly, the effect of PAM to enhance vitallity which is indicated byincreasing in consentration of Te, LH, and FSH has not been fully understood. This study was conducted toelucidate whether treatment of PAM crude extract will be able to enhance vitality which is indicated withincreasing in level of testosteron, LH, and FSH.Design and Method: In the Post Test Control Group Design, 30 male of three month-old Sprague Dawley ratsrandomly assigned into three groups, i.e Group A as a control, Group B, and group C was treated orally withaquadest 2 ml, 1 ml (25 mg), and 2 ml (50 mg) of PAM crude extract respectively for seven days. tetestosteron,LH, and FSH level were assessed by RIA and IRMA respectively. The different level of testosteron, LH, and FSHamong the three groups was analyzed by ANOVA, followed by Post Hoct Tukey HSD.Result: The result showed significantly higher serum testosterone and LH level (p=0.049 and p=0.853respectively) in 50 mg treated rats when compared with control while no significant difference was seen in theserum levels of these two markers between 25 mg treated rats and 50 mg treated rats. Statistical comparisonrevealed no significant difference in FSH level either in the two treated groups as compared to control or 25mgtreated group to 25 mg treated group.Conclusion: Treatment of extract PAM 50 mg could enhance vitality which is indicated with increasing intestosterone and LH level, but had no effect to FSH level, (Sains Medika, 1 (1) : 53-62).Pendahuluan: Pimpinella alpina Molk (PAM) atau purwoceng telah digunakan sebagai obat alternatif secaraturun temurun untuk meningkatkan vitalitas pria. Namun, pengaruh ekstrak PAM terhadap peningkatan vitalitasdengan indikator peningkatan kadar Te, LH, dan FSH masih belum jelas. Penelitian ini bertujuan untukmengetahui apakah pemberian ekstrak PAM mampu meningkatkan vitalitas dengan indikator peningkatankadar testosteron, LH, dan FSH.Metode Peneletian: Post Test Control Group Design dengan sampel 30 tikus jantan Sprague Dawley umur 3bulan dibagi menjadi 3 group secara random masing-masing terdiri dari 10 ekor tikus. Group A, sebagai kontroldiberi aquadest 2 ml, Group B dan Group C masing-masing diberi 1 ml ekstrak PAM (25 mg) dan 2 ml ekstrakPAM (50 mg). Setelah satu minggu aklimatisasi tikus diberi perlakuan selama 7 hari berturut-turut denganacufirm (blunt type needle). Te kemudian diperiksa dengan metode Radio Immuno Assay (RIA), sehingga Teyang diukur adalah Te total (TTe), sedangkan LH dan FSH diperiksa dengan metode Immuno Radiometric Assay(IRMA).Kadar testosteron, LH, dan FSH, masing-masing diuji dengan metode RIA dan IRMA. Perbedaan kadartestosteron, LH, dan FSH di antara group dianalisis dengan ANOVA dan dilanjutkan dengan uji Post Hoct TukeyHSD.Hasil Penelitian: Hasil penelitian menunjukkan bahwa group C mempunyai rerata kadar testosteron lebihtinggi bermakna dibanding group A, namun tidak bermakna dibanding group B. Rerata kadar testosterongroup B lebih tinggi dibanding group A namun tidak bermakna. Rerata kadar LH group C lebih tinggi bermakna dibanding group A, namun tidak bermakna dibanding group B. Rerata kadar LH group B lebih tinggi dibandinggroup A namun tidak bermakna. Rerata kadar FSH group C lebih tinggi dibanding group A dan group B namuntidak bermakna, demikian pula dengan group B dibanding group A.Kesimpulan: Pemberian ekstrak PAM 50 mg mampu meningkatkan vitalitas dengan indikator peningkatankadar Testosteron dan LH, namun tidak meningkatkan kadar FSH, (Sains Medika, 1 (1) : 53-62).
Increase in Bcl2 expression of penile and prostate cells of Sprague Dawley male rats following treatment with buceng (combination of Pimpinella alpina molk with Eurycoma longifolia Jack) Nasihun, Taufiqurrachman
Medical Journal of Indonesia Vol 24, No 1 (2015): March
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (751.331 KB) | DOI: 10.13181/mji.v24i1.1023

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Background: Treatment with buceng combination of Eurycoma longifolia Jack and Pimpinella alpine Molk has been proven to increase testosterone level, decrease apoptosis and caspase3 expression. Bcl2 is an antiapoptotic protein found in cytoplasm which inhibits cells apoptosis. This study was aimed to investigate the effect of buceng on Bcl2 expression on penile and prostate tissues of the rats. Methods: In this experimental study, 24 male Sprague Dawley rats of 90 days old, weighing ± 300 grams, were randomly assigned into four groups. Group A, normal rats. Group B, castrated rats and treated with buceng 100 mg/day, per oral (Cast-Bcg); Group C, castrated rats and treated with 2 ml of water as placebo against buceng (Cast-Plac). Group D, castrated rats, treated with mesterolone 6.75 mg/day, per oral, as exogenous testosterone (Cast-Mest). All rats were treated for 30 days. Manova test was used to analyze the different expression of Bcl2 among groups with significance level at p ≤ 0.05. Results: Castration was associated with significant decrease of Bcl2 expression in the penile and prostate tissues (53.0 and 50.9%, respectively) compared to normal rats (82.6 and 84.2%, respectively, p < 0.001). Treatment with mesterolone reversed Bcl2 expression (77.1 and 78.1%) to a near normal level. The same level of Bcl2 expression was also observed with buceng treatment (73.8 and 78.2%).Conclusion: The treatment with buceng could enhance Bcl2 expression in penile and prostate tissues, comparable to normal rats and mesterolone treated rats.
Calorie Restriction, Stem Cells, and Rejuvenation Approach Nasihun, Taufiqurrachman
Sains Medika Vol 7, No 2 (2016): July-December 2016
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (138.331 KB) | DOI: 10.26532/sainsmed.v7i2.1171

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Aging may be defined as the time-dependent deterioration in function of an organism associated with or responsible for the increase in susceptibility to disease and probability of death with advancing age (Harman, 1981; Cefalu, 2011). Generally, the aging organisms are characterized by both biochemical and functional declines. Declining of basal metabolism rates, protein turnover, glucose tolerance, reproductive capacity, telomere shortening, and oxidative phosphorylation are related to the biochemical. Whilst, lung expansion volume, renal glomerular and tubular capacities, cardiovascular performance, musculoskeletal system, nerve conduction velocity, endocrine and exocrine systems, immunological defenses, and sensory systems are associated with the physiological declining (Baynes and Dominiczak, 2015). Some evidences indicated that, although members of a species develop into adults in the same way, even genetically similar or identical individuals, raised in identical conditions and eating identical food, but they may age differently (Baynes and Dominiczak, 2015). These aging differences are attributable to the life style particularly calorie and dietary restriction intakes, reactive oxygen species (ROS) production, and thus its implication on severity of damage, repair capacity, and error accumulation in cellular genetic material (Baynes and Dominiczak, 2015; Mihaylova et al., 2014; Mazzoccoli et al., 2014). Therefore, in molecular terms, aging can be defined as a decline of the homeostatic mechanisms that ensure the function of cells, tissues, and organs systems (Mazzoccoli et al., 2014). Accordingly, if the homeostatic mechanism can be repaired, the result is rejuvenation.
Flavonoids and Aging Nasihun, Taufiqurrachman
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 7, No 1 (2016): January - June 2016
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (285.629 KB) | DOI: 10.26532/sainsmed.v7i1.998

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Aging is inevitable process, however human comes into aging is in difference way and difference rates. Some of them undergo aging earlier due to degenerative diseases, unable to reach the maximum lifespan and are thus denominated secondary aging (Huebschmann AG, 2011). Some of others undergo aging latter, looked younger than their truely chronological age, have no suffer degenerative disease, able to achieve maximum lifespan and so called primary aging (De la Fuente, 2009; Huebschmann AG, 2011). Maximum lifespan or often called longevity of the species is defined as maximum time that a species belonging to determine how long the species can live, for example maximum lifespan in human beings is 122 years, whereas the lifespan in rats and mouse strains is only 3 and 4 years respectively (De lafuente, 2009). It must be distinguished this longevity from mean or averages longevity. Average longevity can be defined as the average of time that member of population that have been born on the same date live (De la Fuente, 2009). The maximum lifespan is fixed in each species, meanwhile the lifespan of individual subject in the population, even when their genotype are the same and grow in a common environment condition, such as protected from external hazard show marked variability (Kirkwood, 2005).
The Administration of Orange Sweet Potato (Ipomoea Batatas L.) Extract Increases Sperm Consentration, Mortility and Viability in Male Mice Exposed to Cigarette Smoke Rizqy, Lintang Laila; Nida, Nor Anisatun; Noviana, Dwi Ayu; Nasihun, Taufiqurrachman; Widayati, Eni
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 8, No 1 (2017): January - June 2017
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.622 KB) | DOI: 10.26532/sainsmed.v8i1.1624

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Introduction: Ipomoea batatas L (LIP) contains antioxidants beta-carotene, vitamin C and vitamin E. Cigarette smoke containing ROS has been proven to decrease sperm quality.Objective: this study aimed to confirm that orange sweet potato extract increases sperm consentration, motility, and viability in mice exposed to cigarette smoke.Methods: In this experimental study with post test only control group design, 30 male mice were divided into 6 groups. Normal group (Nor-G), no intervention, served as the normal control group. Negative control group (Neg-G) was only exposed to cigarette smoke. Positive control group was only treated with IPL 16mg/ml. IPL-15, IPL-16, and IPL-17 group were treated with IPL 15, 16, and 17mg/ml and exposed to cigarette smoke, respectively.Results: Post Hoc LSD analysis showed that means of sperm concentration and viability in IPL-15, IPL-16, and IPL-17 group were significantly higher compared to that of Neg-G (p < 0.05) and were lower compared to that of Nor-G and Pos-G (p < 0.05). Mann Whitney analysis indicated that the persentage of sperm motility in IPL-15, IPL-16, and IPL-17, were significant higher compared to that of Neg-G, p < 0.05. Whilst compared to that of Nor-G and Pos-G the persentage of sperm motility in IPL-15, IPL-16, and IPL-17, there was no significant differences, p > 0.05.Conclusion: treatment of IPL capable of increasing sperm concentration, motility, and viability in male mice were exposed to cigarrette smoke.
Successful Aging Nasihun, Taufiqurrachman
Sains Medika Vol 6, No 1 (2015)
Publisher : Fakultas Kedokteran UNISSULA

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Administration of Mung Bean Extract (Phaseolus radiatus) in Increasing Hb and Ferritin Level and Decreasing Malondyaldehide (MDA) Level in Anaemic Rats Wijayanti, Heni; Nasihun, Taufiqurrachman; Hussaana, Atina
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 8, No 2 (2017): July-Desember 2017
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (33.411 KB) | DOI: 10.26532/sainsmed.v8i2.1981

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BACKGROUND: Mung bean, not only contains protein, carbohydrates, and fats, but also contains iron and vitamin C which are proven to overcomeanemia in pregnant woman. High iron levels are potential to increase ROS production through fenton reaction. OBJECTIVE: to prove the administrationof mungbean can increase Hb and ferritin levels and decreasing malondihaldehyde (MDA) in anaemic rats.METHODS: This research uses Post Test Only Control Group Design. A total of 25 anaemic rats were divided into 5 groups: normal group (Nor-G);negative control group (Neg-G), were given low Fe diet; and treatment group with low Fe and mung bean extract at dose of 0,18g/200g/days (GP-0,18),0,36g/200g/days (GP-0.36), dan 0,72g/200g/days (GP-0.72). Low Fe diet and mung bean extract were administered for 14 days. Hb levels were measuredusing Sahli method, ferritine level using Immulite Ferritine Kit, and MDA were measured using TBA.RESULTS: Compare to Nor-G and Neg-G, levels of ferritin and Hb on groups with mung bean extract 0,18g (75.56), 0,36g (90.98) and 0,72g (95.87)were significantly higher (p < 0.05). While MDA levels on groups with mung bean 0,18g (4.646), 0,36g (3.396) and 0,72g (1.92) were significantly lowerthan on Nor-G and Neg-G groups (p < 0.05).CONCLUSION: The administration of mung bean extract can increase Hb and ferritin, also lower MDA level on anaemic rats.
Ramadan Fasting, Health, and Autophagy: Is There any Relationship? Nasihun, Taufiqurrachman
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 8, No 2 (2017): July-Desember 2017
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.18 KB) | DOI: 10.26532/sainsmed.v8i2.3135

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Fasting, the voluntary abstinence from food intake for a certain period of time is a well-known practice in relation with spiritual purposes (Trepanowski, JF, 2010). Ramadan fasting (RF) is an obligation of Muslim across the world during the entire holy month of Ramadan. In this specified period, Muslim every day from dawn to dusk are prohibited to eat, drink, and sexual relation, by which Muslim seek an enhanced level of closeness to God (Alloh). This testament is referenced in the verse of Al Baqoroh 183 of Holy Qur?an: ?O you who believe! Fasting is prescribed for you, as it was prescribed for those before you, that you may become righteous?. In addition to closeness, RF in the Islamic perspective also believed capable of improving human health as prophet of Mohammad (peace be upon him) said that ?fasting makes you healthier?. However, to the benefit of RF during the entire month of Ramadan on health remain circumstantial. There is germane question regarding RF, whether or not RF can elicit benefits or otherwise will compromise health status and physical activity performances in fasted people, considering total calorie and fluid intake were reduced (Aziz AR, et al. 2012; Bouhlel E, et al. 2006). Accordingly, in the last two decades, the health effects of RF have recently been the subject of scientific research, not only in health outcome (Trepanowski, JF, 2010; Moro T, et al. 2016), but also in exercise performance (Aziz AR, et al. 2012).
Stem Cell Mesenchymal Injection Increases Platelet-Derived Growth Factors Level and Percentage of Collagen in Third-Degree Burn injured Mice Ridawati, Erna; Nasihun, Taufiqurrachman; Wiratno, Wiratno
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 7, No 2 (2016): July-December 2016
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1021.094 KB) | DOI: 10.26532/sainsmed.v7i2.1173

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Introduction: Mesenchymal stem cell (MSC) has been demonstrated to accelerate wound healing. Platelet-derived growth factor (PDGF) and collagen have an important role in wound healing. However, the effect of MSC on levels of PDGF and number of collagen has not been established. Objectives: To determine the effect of MSC on the levels of PDGF and the percentage of collagen in the burns healing. Methods: in this experimental study, 20 BALB /c mice were divided into 4 groups: a control group (G-0) injected with 2 ml of distilled water and group 1 (G-1), Group 2 (G-2), and group 3 (G- 3) injected with MSC at the dose of 1 x 104, 2 x 104, and 4 x 104 respectively. The burn wound was made by attaching a metal nail that had been heated for 20 seconds at the feet of mice. Levels of PDGF on day 2 (PDGF-2) and 7 (PDGF-7) was assessed by ELISA, while on day 10 excision of skin was done to calculate the percentage of collagen. Results: There was a significant difference in the levels of PDGF-2, PDGF-7, and the percentage of collagen ( P <0.05). Posthoc analysis showed that the levels of PDGF-2, PDGF-7, and percentage of collagen in the G-2 and G-3 was significantly higher than that of G-0, p <0.05. While the levels of PDGF-2, PDGF-7, and the percentage of collagen in the G-3 was significantly higher than that of G-2 and G-1 (p <0.05). Conclusion: Injection of MSC at a dose of 2 x 1044 and 4 x 1044 increases the levels of PDGF and the percentage of collagen.
Effect of Celery Extract Administration on 8-OHdG and Number of Foam Cell in Wistar Strain Rats With A High-Fat Diet Budiarto, Agnes; Nasihun, Taufiqurrachman; Hussaana, Atina
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 8, No 2 (2017): July-Desember 2017
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (520.637 KB) | DOI: 10.26532/sainsmed.v8i2.2421

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BACKGROUND: A high fat diet, accompanied by high frequency eating, results in hyperlipidemia. One prevention for hyperlipidemia is administration of celery extract.OBJECTIVE: to explore the effect of celery extract on the level of 8-OHdG (8 -Hydroxy-2?-Deoxy Guanosine) and the number of foam cell.METHODS: Experimental studies with post-test only control group design used Wistar rats, administered with adrenaline and high-fat diet forhyperlipidemic induction.Total of 35 rats were divided into 5 groups: negative controls (Neg-G); positive control treated with simvastatin (Nor-G); ES25-G,ES50-G, and ES75-G group were treated with 25 mg/200 g BW, 50 mg/200 g BW, and 75 mg/200 g BW celery extract, respectively. The treatment was given for 30 days. The 8-OHdG level was determined by ELISA, whereas the number of foam cells was determined by histopathologic preparations with HE staining. Data analysis was conducted by Kruskal-Wallis and Mann-Whitney.RESULTS: Mann-Whitney analysis showed levels of 8-OHdG in ES-25-G 12.13±0.21, ES-50-G 7.23±0.25, ES-75-G 4.41±0.45 significantly lowerthan the Nor-G 14.30±0.66, respectively p<0.001. The number of foam cells in the ES-25-G was 7.57±0.53, ES-50-G 6.57±0.79, ES-75-G 3.43±0.53,significantly lower than the Neg-G 13.57±1.27, respectively p<0.001.CONCLUSION: Celery extract capable of decreasing levels of 8-OHdG and the number of foam cells in Wistar rats induced with adrenaline and high-fatdiet.