Zainul Muttaqin
Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Teknologi Sepuluh Nopember (ITS)

Published : 18 Documents
Articles

Found 18 Documents
Search

SEORANG PENDERITA HEPATITIS KRONIK B DAN C DENGAN MUTASI PADA GEN P53 KODON 249 PADA JARINGAN HATI Eka Saputra, I Wayan; Gunawan, Stephanus; Muttaqin, Zainul; Soemoharjo, Soewignjo
journal of internal medicine Vol. 8, No. 2 Mei 2007
Publisher : journal of internal medicine

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Role of gene P53 as a tumor suppressor gene in generating primary liver cancer is very important primarily in cellgrowth regulation and apoptosis. By using AS-PCR method, the gene mutation can be recognized. It has been reported a chronichepatitis B and C case with experiencing mutation of gene P53 Codone 249 in liver tissue of a 33 years old male with anti HCVpositive, HBsAg positive, on the liver biopsy indicated of chronic hepatitis without cirrhotic features and malignancy signs. OnAS-PCR examination found mutation on gene P53 codone 249. This finding was expected to be an early warning sign foroccurrence of primary liver cancer, so that, early intervention could be performed.
Prevalensi dan Derajat Infeksi Anisakis sp. pada Saluran Pencernaan Ikan Kakap Merah (Lutjanus malabaricus) di Tempat Pelelangan Ikan Brondong Lamongan Muttaqin, Zainul; Abdulgani, Nurlita
Jurnal Sains dan Seni ITS Vol 2, No 1 (2013)
Publisher : Jurnal Sains dan Seni ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penelitian ini bertujuan untuk mengetahui prevalensi dan derajat infeksi Anisakis sp. yang ditemukan pada saluran pencernaan ikan kakap merah (Lutjanus malabaricus) di tempat pelelangan ikan Brondong Lamongan berdasarkan panjang total ikan. Spesimen ikan dibedah dengan cara dibuat sayatan pada bagian ventral ikan. Organ usus dan lambung diletakkan ke dalam cawan petri yang berisi larutan fisiologis NaCl 0,9% dan diidentifikasi dengan menggunakan mikroskop cahaya. Hasil pengamatan menunjukkan bahwa ikan kakap merah telah terinfeksi larva 3 (L3) Anisakis sp.. Prevalensi dan derajat infeksi ikan kakap merah berukuran 25-37 cm lebih besar dibandingkan dengan ikan kakap merah yang berukuran 21-24 cm. Nilai prevalensi ikan berukuran 21-24 cm sebesar 66,67 % dengan nilai derajat infeksi sebesar 5,2 parasit/ikan sedangkan pada ikan berukuran 25-37 cm nilai prevalensinya sebesar 80 %. Dengan nilai derajat infeksi sebesar 18,25 parasit/ikan. Berdasarkan hasil uji statistik menggunakan Uji T menunjukkan bahwa, jumlah Anisakis sp. pada saluran pencernaan ikan kakap merah berhubungan nyata dengan panjang tubuhnya (P<0,05).
PENGEMBANGAN LKS BERBASIS INQUIRI MENGGUNAKAN SOFTWARE GEOMETER’S SKETCHPAD PADA MATERI HUBUNGAN ANTAR SUDUT PADA GARIS SEJAJAR DIPOTONG GARIS LAIN MUTTAQIN, ZAINUL; Amin, Siti Maghfirotun
MATHEdunesa Vol 2, No 1 (2013)
Publisher : Jurusan Matematika UNESA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pendekatan, strategi, metode, dan teknik pembelajaran pada hakikatnya dapat dibedakan tetapi tidak dapat dipisahkan. Keempat istilah tersebut merupakan satu kesatuan dalam pembelajaran. Pendekatan, strategi, metode, dan teknik pembelajaran yang akan dan/atau sedang digunakan dapat diketahui dari langkah-langkah pembelajaran yang telah tersusun dan/atau sedang terjadi. Pendekatan pembelajaran adalah cara umum dalam memandang pembelajaran. Sedangkan strategi pembelajaran adalah ilmu dan kiat di dalam memanfaatkan segala sumber belajar yang dimiliki dan/atau yang dapat dikerahkan untuk mencapai tujuan pembelajaran yang telah ditetapkan. Metode mengajar adalah berbagai cara kerja yang bersifat relatif umum yang sesuai untuk mencapai tujuan pembelajaran tertentu dan teknik pembelajaran adalah ragam khas penerapan suatu metode sesuai dengan latar penerapan tertentu. Teknik pembelajaran mengambarkan langkah-langkah penggunaan metode mengajar yang sifatnya lebih operasional. Faktor-faktor yang perlu diperhatikan dalam penentuan teknik pembelajaran di antaranya adalah kemampuan dan kebiasaan guru, ketersedian sarana dan waktu, serta kesiapan siswa. Faktor-faktor yang perlu dipertimbangkan dalam memilih strategi pembelajaran ialah tujuan pembelajaran, jenis dan tingkat kesulitan materi pelajaran, sarana, waktu yang tersedia, siswa, dan guru.
Nyale Sea Worm As Antibacterial Substances Dyah Jekti, Dwi Soelistya; Purwoko, Agus Abhi; Muttaqin, Zainul
Jurnal ILMU DASAR Vol 9, No 2 (2008)
Publisher : Fakultas MIPA Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.442 KB)

Abstract

Nyale is a sea worm that belongs to class of polychaete. It appears on a huge crowd, usually five days after the monsoon in February, at the surface of the sea for breeding. The colors of the female and male worms are green and brown, respectively. The worms are collected in nyale season, freezed-dryer, and extracted with ethyl acetate. Antimicrobial activity properties of the male worm extract are carried out toward benthos bacteria and clinical isolate bacteria using ciprofloxacin as comparing agent. The results show that, after colom chromatography, fraction number 1 and 4 have the best antibacterial activities (broadest spectrum) toward clinical isolate bacteria. All eleven fractions show also antibacterial activities toward nine benthos bacteria. The minimum inhibitory concentration (MIC) of fractions 1 and 4 toward six clinical isolate bacteria is 100 μg/ml. Meanwhile, fraction 4 exhibits two peaks in its HPLC chromatrogram.Keywords : Nyale, sea worm, antibacterial substances
Detection of Hepatitis B Virus Pre-core Mutant by Allele Specific Polymerase Chain Reaction Soemohardjo, Soewignjo; Widita, Haris; Muttaqin, Zainul; Gunawan, Stephanus; Wijaya, Mahendra; Wiguna, Putu Aditya; Rhamdiani, Shelly Olivia
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 14, NUMBER 1, April 2013
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24871/141201319-23

Abstract

Introduction: Mutation in pre-core region is characterized by negative HBeAg and positive anti-HBe despite active replications of the virus. The mutation has diagnostic and prognostic implications. Therefore, detectionof pre-core mutant is important. Standard diagnosis approach for detecting pre-core mutant is through DNA sequencing of hepatitis B virus (HBV) pre-core region. Unfortunately, DNA sequencing is not available in mostcenters. Hence, a simpler diagnostic approach is necessary.Method: An observational-analytic design study was performed. Detection of pre-core mutant was conducted in individuals with positive HBsAg and HBV DNA that had various patterns of HBeAg and anti HBe. HBsAg, HBeAg and anti-HBe was detected using immunochromatography technique. The HBV DNA was evaluated by using qualitative polymerase chain reaction (PCR) testing. PCR was done by three rounds of amplification with primers derived from wild type pre-core and mutant pre-core. Results: Of 25 sera with HBeAg negative, anti-HBe positive and HBV DNA positive, allele specific (AS) PCR pre-core mutant was detected in 20 (80%) sera. Two sera with HBeAg negative, anti HBe negative and HBV DNA positive were negative for pre-core mutant. Of 8 sera with HBeAg positive, anti HBe negative and HBV DNA positive, pre-core mutant was detected in 2 (25%) sera.Conclusion: Most of individuals with HBV DNA positive, HBeAg negative and anti-HBe positive have harbored pre-core mutant. The finding indicated that all patients with HBsAg positive, HBV DNA positive and HBeAg negative, but anti-HBe positive should be examined for the presence of pre-core mutant. Pre-core mutant is also found in HBeAg positive individual. Keywords: HBV, pre-core mutant, polymerase chain reaction
Detection of HBV-DNA and Its Correlation with the HBeAg/Anti-HBe Serological Status in HBsAg-positive Patients Widita, Haris; Soemohardjo, Soewignjo; Muttaqin, Zainul; Wiguna, Putu Aditya; Rhamdiani, Shelly Olivia; Wijaya, Mahendra; Gunawan, Stephanus
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 13, NUMBER 2, August 2012
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: In the past years, HBeAg and anti-HBe status in individuals with positive HBsAg were often correlated to viral replication. This study was aimed to find correlation between the HBV viremia and HBeAg/anti-HBe serological status in HBsAg-positive individuals. Method: An observational-analytic design was performed in this study. The sera of all positive HBsAg patients at Biomedika Hospital Laboratory were collected and examined for HBeAg and anti-HBe using immunochromatography technique between January and April 2012. The sampling method was purposive sampling. Afterwards, the sera were examined for HBV-DNA by polymerase chain reaction (PCR). Results: Sufficient amount of sera were collected from 44 patients consisting of 33 males and 11 females. The mean age was 15-68 years. Positive HBeAg and negative anti-HBe status was found in 11 (42%) patients. Negative HBeAg and positive anti-HBe was found in 26 (59.1%) patients. Both HBeAg and anti-HBe were negative in 7 (16.3%) patients. HBV-DNA was detected in all 11 (100%) patients with positive HBeAg and negative anti-HBe. HBV-DNA was also detected in 11 (42%) patients with negative HBeAg and positive anti-HBe. However, there was only one patient (14.3%) with both negative HBeAg and anti-HBe status, who had detectable HBV-DNA. Conclusion: Positive HBeAg can be used as an indicator of viremia, but negative HBeAg cannot be used as an indicator of the absence of viremia without further HBV-DNA testing. Patients with negative HBeAg and positive HBV-DNA were suspected for having pre-core mutant. Keywords: HBV-DNA, positive HBsAg, HBeAg, anti-HBe, pre-core mutant
The Detection of H pylori In Gastric Mucosal Biopsy Specimens by PCR Using Primers Derived From Ure C Gene in Patients with Dyspepsia Soemohardjo, Soewignjo; Palgunadi, I Gede; Gunawan, S; Muttaqin, Zainul; Widita, Haris; A, Wenny Astuti
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 8 ISSUE 2 August 2007
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: The detection of Helicobacter pylori (H. pylori) in gastric biopsy specimens can be done using CLO (Campylobacter Like Organism) test and histopatological examination, but the sensitivity of both Method is influenced by the density of the bacteria in the sample. Beside that, the coccoid form is detected with difficulty by histology and need immunohistochemical stain to confirm. PCR can be used for the detection of both spiral and coccoid form of the bacteria. Objective: To detect the genome of H. pylori by Polymerase Chain Reaction (PCR) using primers derived from ureC gene of the bacteria in gastric biopsy specimen from patients with dyspepsia. Methods: Gastric biopsy specimen from 179 patients with dyspepsia in the endoscopic unit Mataram hospital. The biopsy was taken from antrum and corpus and put into sterile saline for the culture of H. pylori and put into 70% ethanol solution for the PCR. The specimen for bacterial culture was carried soon to microbiology laboratory and plated into the appropriate media and grown in microaerophilic condition in CO2 incubator. The PCR was done using primers derived from ureC. Result: The H. pylori genome was detected in 79 of 179 biopsy sample (44.13%). The bacterial culture was positive for H. pylori in 22 (12%). The PCR result was positive in 10/35 of patient with normal endoscopy (28.57%). From 22 patients with duodenal ulcer without gastric ulcer the PCR was positive in 15 (68.18%). In patient with gastric ulcer without duodenal ulcer the PCR was positive in 9 patients (42.08%). From 7 patient with combined gastric and duodenal ulcer the PCR was positive in 5 (71.43%), in 3 patient with gastric cancer the PCR was positive in 1 (33.33%). Conclusion: The study showed that 44.13% of patient with dyspepsia in Mataram hospital was positive for H. pylori by PCR. Keywords: detection of Helicobacter pylori, gastric mucosal biopsy specimen, polymerase chain reaction, ureC gene
The Absence of Urease Enzymatic Activity of Helicobacter pylori Coccoid Form Jekti, Dwi Sulistya Dyah; Soemohardjo, Soewignjo; Muttaqin, Zainul
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 9, ISSUE 2, August 2008
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Helicobacter pylori (H. pylori) is a gram negative and pleomorphic bacteria that able to change its morphology according to the environment. The objective of the study was to determine the biochemical and some genetic characteristic of coccoid form of H. pylori induced by starvation, aerobiosis and antibiotic. Method: The material of the study is an isolate of spiral form of CagA positive H. pylori grown from gastric biopsy specimen of a patient with chronic gastritis. The CagA positive isolate was subcultured in liquid media containing the sheep sera. The sample was divided into three groups each group consist of 27 tube. Each tube contained 109 CFU of H. pylori bacteria/ml in 4 ml liquid media. So the experiment was performed in 3 replicates. In the first group of sample, coccoid form was induced by a prolonged culture under microaerophilic condition without the addition of fresh media, in the second group by aerobiosis, while in the third group by addition of 0.1 µg amoxycillin/ml cultured in microaerophilic condition. Periodic sampling was done every day to calculate the percentage of coccoid form, to observe the possibility to regrow the spiral form and for serial electron microscopic observation. One tube is picked up in every periodic sampling. In tubes containing antibiotic the periodic sampling was done one hourly. Detection of cagA and ureA gene was done by Polymerase Chain Reaction (PCR) with appropriate primers. Results: The time needed for the development of coccoid form: Length of time from the start of the experiment needed to reach 100% coccoid form was: 49 days in microaerophilic with starvation, 28 days in aerobiosis with starvation, and 13.5 days in antibiotic. result of biochemical test: Urease enzymatic activity was only positive in spiral form. All samples of coccoid form due to all the 3 stressors did not show any urease enzymatic the activity. PCR of ureA gene: All samples of spiral and coccoid form showed positive band of ureA gene and cagA gene. Western blot of protein CagA, urease A and urease B: Western blot analysis showed that in spiral form and all coccoid form band of urease A and urease B is clearly seen,while cagA in Western blot only clearly seen in spiral form but it is absent in cocoid form. Conclusion: Troughout the cycle of coccoid form the urease gene responsible for the production of urease and cagA gene responsible for virulence was in intact condition. However, despite the presence of urease protein in coccoid form the urease enzymatic activity was absent. This fact has several diagnostic and clinical implications. Keywords: urease enzymatic activity, coccoid form, Helicobacter pylori
Detection of Hepatitis B Virus Pre-core Mutant by Allele Specific Polymerase Chain Reaction Soemohardjo, Soewignjo; Widita, Haris; Muttaqin, Zainul; Gunawan, Stephanus; Wijaya, Mahendra; Wiguna, Putu Aditya; Rhamdiani, Shelly Olivia
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 14, NUMBER 1, April 2013
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Introduction: Mutation in pre-core region is characterized by negative HBeAg and positive anti-HBe despite active replications of the virus. The mutation has diagnostic and prognostic implications. Therefore, detectionof pre-core mutant is important. Standard diagnosis approach for detecting pre-core mutant is through DNA sequencing of hepatitis B virus (HBV) pre-core region. Unfortunately, DNA sequencing is not available in mostcenters. Hence, a simpler diagnostic approach is necessary.Method: An observational-analytic design study was performed. Detection of pre-core mutant was conducted in individuals with positive HBsAg and HBV DNA that had various patterns of HBeAg and anti HBe. HBsAg, HBeAg and anti-HBe was detected using immunochromatography technique. The HBV DNA was evaluated by using qualitative polymerase chain reaction (PCR) testing. PCR was done by three rounds of amplification with primers derived from wild type pre-core and mutant pre-core. Results: Of 25 sera with HBeAg negative, anti-HBe positive and HBV DNA positive, allele specific (AS) PCR pre-core mutant was detected in 20 (80%) sera. Two sera with HBeAg negative, anti HBe negative and HBV DNA positive were negative for pre-core mutant. Of 8 sera with HBeAg positive, anti HBe negative and HBV DNA positive, pre-core mutant was detected in 2 (25%) sera.Conclusion: Most of individuals with HBV DNA positive, HBeAg negative and anti-HBe positive have harbored pre-core mutant. The finding indicated that all patients with HBsAg positive, HBV DNA positive and HBeAg negative, but anti-HBe positive should be examined for the presence of pre-core mutant. Pre-core mutant is also found in HBeAg positive individual. Keywords: HBV, pre-core mutant, polymerase chain reaction
Nyale Sea Worm As Antibacterial Substances Dyah Jekti, Dwi Soelistya; Purwoko, Agus Abhi; Muttaqin, Zainul
Jurnal ILMU DASAR Vol 9 No 2 (2008)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.442 KB)

Abstract

Nyale is a sea worm that belongs to class of polychaete. It appears on a huge crowd, usually five days after the monsoon in February, at the surface of the sea for breeding. The colors of the female and male worms are green and brown, respectively. The worms are collected in nyale season, freezed-dryer, and extracted with ethyl acetate. Antimicrobial activity properties of the male worm extract are carried out toward benthos bacteria and clinical isolate bacteria using ciprofloxacin as comparing agent. The results show that, after colom chromatography, fraction number 1 and 4 have the best antibacterial activities (broadest spectrum) toward clinical isolate bacteria. All eleven fractions show also antibacterial activities toward nine benthos bacteria. The minimum inhibitory concentration (MIC) of fractions 1 and 4 toward six clinical isolate bacteria is 100 μg/ml. Meanwhile, fraction 4 exhibits two peaks in its HPLC chromatrogram.