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Immuncontraception Reversibility Test of Goat Zona Pellucida-3 (gZP3) on Mice (Mus musculus)

HAYATI Journal of Biosciences Vol 13, No 4 (2006): December 2006
Publisher : Bogor Agricultural University, Indonesia

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Abstract

The aim of this study was to prove the reversibility of gZP3 protein on mice as an animal model. Treatment group of mice (Mus musculus) were immunized with 40 ug goat zona pellucida-3 (gZP3) as an anti fertility agent. Control group of mice were injected only with physiologic solution. The mice were injected three times, the first injection as an immunization. The second and third injected as a booster with 14 days interval. The first injection, gZP3 suspension was diluted in complete Freund’s adjuvant (CFA). The boosters, gZP3 suspension was diluted in incomplete Freund’s adjuvant (IFA) 1:1 (v/v). Blood samples were collected before immunization, seven days after the second booster and at the time of parturition. Seven days after the last injection, the mice were mated, and the parturition was observed after that. The result showed that antibody titer of immunized mice serum was increasing (P < 0.05) seven days after immunization, and decreasing (P > 0.05) after parturition compared to antibody titer of serum before immunization. Dot blotting analysis showed that gZP3 protein could recognize gZP3 antibody of the immunized mice. Protein of gZP3 as an immunocontraceptive substance was reversible in 91.60 + 4.90 days. This reversibility was longer (P < 0.05) compared to control mice i.e. 26.50 + 4.30 days. Key words: immunocontraception, goat zona pellucida-3, reversibility, mice, animal model, gZP3 antibody

The Aplication of Synthetic Progesterone on Fries Holland Dairy Cattle After Artificial Insemination and in Madura Cattle Embryos

Media Veteriner Vol 6, No 1 (1999): Media Veteriner
Publisher : Media Veteriner

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Abstract

Forty healthy and non-pregnant Fries Holland dairy cows which had received oestrus synchronization were divided randomly into four experimental groups. Artificial inseminations (AI) were implemented 8-10 hours post estrous using frozen semen from the same breed. The first three groups were injected with 100, 150 and 200 mg medroxy progesterone acetate (MPA) i.m., respectively, three days post AI, while the other group was injected with placebo as control group. Madura cattle embryos were transfered contralaterally seven days post AI. The percentage of pregnancy in groups which had received 100 and 150 mg MPA was 37.5%r, and in group received 200 mg MPA and control was 55.6%, and 60%, (P>0.05), respectively. Calving percentages of the 100, 150, 200 mg MPA and the control groups were 25,37.5,55.6 and 60% respectively (P>0.05). All birth gave single calve.

BOM BUNUH DIRI: ANTARA JIHAD DAN TEROR (Meluruskan Pemahaman Hukum Bom Bunuh Diri)

Jurnal Al-Manahij Vol 5, No 1 (2011)
Publisher : STAIN Purwokerto

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Abstract

Policy of western countries, especially the United States against some Muslim countries in the Middle East region led to a backlash and generate assistance in various ways. One way or form of resistance which is by suicide bombing. Even this kind of action does not only happened in the region or the state which is being colonized or war zone, but also often happened in a peaceful country like Indonesia. This paper attempt to uncover the law suicide bombing in perspective of Islamic law by classifying into two, the first suicide bombing as a jihad and second, suicide bombings as terror. This article tries to answer the questions when suicide bombing can be categorized as jihad? What are the conditions or requirements? And when its categorized as terror? The answer in this paper derived from qualitative research. This research is library research toward source texts of Islam, including the fatwas and opinions of ulama, especially contemporary ulama. Author using a theory of maMlahah which is the purpose of shari´a (maqashid al-syariah) as basis for reviewing the legal and categorized of suicide bombing. When a suicide bombing itself include maMlahah by meeting various condition or requirements, then it is categorized as jihad. However, when its performed in peaceful area causes damage that reduces maMlahah, suicide bombing is categorized as terror.Kata kunci: bom bunuh diri, jihad, teror, maMlahat.

Ijtihad Kontemporer Sebagai Upaya Pembaruan Hukum Keluarga Di Indonesia

Jurnal Al-Manahij Vol 7, No 2 (2013)
Publisher : STAIN Purwokerto

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Abstract

Perkembangan zaman yang berimplikasi pada lahirnya berbagai fenomena dan persoalan hukum membutuhkan jawaban. Banyak persoalan yang tidak terpenuhi/teratasi oleh aturan hukum Islam dan hukum keluarga di Indonesia. Oleh karena itu, perlu ijtihad kontemporer dalam rangka menjawab berbagai persoalan aktual tersebut. Artikel ini memaparkan tentang urgensi ijtihad kontemporer dalam rangka pembaruan hukum keluarga di Indonesia. Ijtihad kontemporer dilakukan dengan mensinergikan metode usul fikih klasik dengan metode ilmiah modern. Banyak persoalan yang harus diakomodir dalam rangka menghasilkan hukum keluarga yang compitable dengan kehidupan masyarakat modern saat ini, seperti kesetaraan jender, HAM, perkembangan sains dan teknologi modern serta perkembangan sosio-kultural masyarakat. Ada beberapa model ijtihad yang biasa digunakan ulama dalam menjawab permasalahan dalam kehidupan masyarakat. Ijtihad yang paling mungkin dan cocok dilakukan dalam rangka melakukan pembaruan hukum keluarga di Indonesia adalah dengan menggunakan metode mu’tadil mutawazin atau metode istislahi, karena metode ini mempertemukan maslahat menurut nass dan maslahat manusia dalam kehidupan bermasyarakat. Kata kunci:    persoalan aktual, ijtihad kontemporer, pembaruan hukum keluarga, maslahat  The advancement in many fields of human life has led to the emergence of law problems which are not covered by the existing laws. So it is the case with the family law in Indonesian context. A contemporary ijtihad is, then, needed to answer those law problems. This article exposes the urgent need of the contemporary ijtihad to redefine the family law in Indonesian context. The contemporary ijtihad is conducted through the combination of classic ushul fiqh method and the scientific modern method. Many problems need to be moderated to generate family law which is compatible with the modern society such as gender issues, human rights, advanced science and technology, and socio-cultural issues. There are several ijtihad methods commonly used by Muslim scholars (ulama) to answer law problems. This writing shows that the most suitable ijtihad method to redefine the family law problems within Indonesian context is mu’tadil mutawazin method or the istislahi method, because this method matches maslahat based on scriptures (nass) and maslahat found in the society. Keywords:     actual problem, contemporary ijtihad, redefined family law, maslahat

Dampak Putusan Mahkamah Konstitusi Mengenai Pasal 43 Ayat (1) Undang-Undang Perkawinan No 1 Tahun 1974 Terhadap Hukum Keluarga Di Indonesia

Jurnal Al-Manahij Vol 6, No 2 (2012)
Publisher : STAIN Purwokerto

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Abstract

Putusan Mahkamah Konstitusi Jumat 17 Februari 2012 terkait dengan anak luar nikah yang disebutakan dalam pasal 43 ayat (1) UU No 1 Tahun 1974 tentang perkawinan menyebutkan bahwa "anak yang dilahirkan di luar perkawinan mempunyai hubungan perdata dengan ibunya dan keluarga ibunya serta dengan laki-laki sebagai ayahnya yang dapat dibuktikan berdasarkan ilmu pengetahuan dan teknologi dan/atau alat bukti lain menurut hukum mempunyai hubungan darah, termasuk hubungan perdata dengan keluarga ayahnya". Perubahan pasal ini harus difahami secara obyektif dan bijak agar membawa maslahat bagi umat dan bukan sebaliknya. Melalui perubahan ini MK berupaya  untuk menuangkan hasil ijtihadnya agar anak-anak yang lahir di luar nikah tetap memiliki hak dan kedudukan yang sama dengan anak-anak lain dan agar tidak terjadi perlakuan diskriminatif terhadap anak-anak tak berdosa ini.This article analyzes the impact of Constitutional Court Decision No. 46/PUU-VIII/2012 that changes Article 43(1) of Law No.1/1974 on Marriage. This Analysis is based on the data obtained from the literature study by using juridical approach. The result of the discussion and analysis concluded that, based on that decision, then every child born has a civil relationship with his father and his fathers family that includes: (1) nasab relationship, (2) the relationship of mahram, (3) the relationship of rights and obligations, (4) the relationship of inheritance (inherit each other) (5) a trust relationship. The Constitutional Court also stated that the validity of the marriage does not depend on the registration of marriage, but based on the requirements of the religion of each party conducting the marriage. Those decisions have an impact on the position of wife and children of unregistered marriages. The wife and children have a civil relationship as registered marriage, it is just not a letter or proof of marriage certificate, but by evidence based on science and technology and/or another evidence. Kata kunci: anak luar nikah, legalisasi  zina, nasab dan  qiyafah

Antibody of goat zona pellucida-3 (gzp3) protein of mice(Mus musculus) block in vitro fertilization of mice as an animal model= Antibodi protein zona pelusida-3 kambing (gZP3) asal mencit(Mus musculus) mencegah ...

Jurnal Sain Veteriner Vol 24, No 1 (2006)
Publisher : Fakultas Kedokteran Hewan

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Abstract

The researchs of immunocontraception have done in ZP3 of several species, but have not been done in ZP3 of goat. In preliminary study, gZP3 protein was effective prohibited of graviditation of mice. The aim of this study was to prove the potency of gZP3 protein to prohibit in vitro fertilization of mice as an animal model. Antibody of gZP3 produced on mice. Immunized mice serum was analyzed using Elisa and Dot blotting method. Antibody of gZP3 supplemented into M-16 media for oocyte incubation, continued with in vitro fertilization. The result showed that antibody titer of immunized mice serum was higher (p

Potensi Protein Reseptor Fertilisasi Zona Pelusida Kambing Sebagai Kandidat Imunokontrasepsi dengan Fertilisasi in vitro pada Sapi (POTENTIAL OF FERTILIZATION RECEPTOR PROTEIN GOAT ZONA PELLUCIDA AS CANDIDATE OF IMMUNOCONTRACEPTION BY USING IN VITRO FER

Jurnal Veteriner Vol 14, No 4 (2013)
Publisher : Jurnal Veteriner

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Abstract

Studies on the role of zona pellucida glycoprotein-3 (ZP3) in immunocontraception have been conductedin many species including goats (gZP3). Our previous study indicated that gZP3 effective in preventingpregnancy in mice. The aim of this study was to prove the evidence of cross reaction in gZP3 to preventfertilization in cow in vitro. Antibody of gZP3 was produced in mice. Immunized mice serum was analyzedusing ELISA technique and dot blot method. Sperm from frozen semen were processed then incubated incapacitation media supplemented with the gZP3, whilst the cow oocyte was incubated in maturationmedia supplemented with antibody of gZP3. Following this, the normal  in vitro fertilization (withoutincubation neither in gZP3 nor in gZP3 antibody) was performed, respectively. The results showed thatantibody titer of immunized mice serum was higher (p<0.05) than the control group. Dot blotting analysisshowed that the antibody of immunized mice were able to recognize gZP3 protein. The serum of immunizedmice which was supplemented in the maturation media of cow oocyte were able to decrease the cleavagerate (p<0.05). Protein gZP3 which was supplemented in the capacitation media of the sperm also coulddecrease the cleavage rate (p<0.05). It is concluded that goat-ZP3 protein may produce cross reaction incow.

KEKERASAN DALAM RUMAH TANGGA DALAM PERSPEKTIF HUKUM POSITIF DAN HUKUM ISLAM (Studi Komparasi antara Undang-undang No. 23 Tahun 2004 tentang Penghapusan Tindak Kekerasan dalam Rumah Tangga dan Ketentuan dalam Fikih Islam)

Pena Justisia Jurnal Media Komunikasi Dan Kajian Hukum Vol 7, No 14 (2008): Pena Justisia Jurnal Media Komunikasi Dan Kajian Hukum
Publisher : Pena Justisia Jurnal Media Komunikasi Dan Kajian Hukum

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Abstract

Kekerasan dalam rumah tangga (KDRT) sebagai salah satu jenis kekerasan yangberbasis gender dari waktu ke waktu terus meningkat. Hal ini pertama dilatarbelakangi oleh budaya patriarki yang terus langgeng, kesetaraan gender yangbelum nampak serta nilai budaya masyarakat yang selalu ingin hidup harmonissehingga cenderung selalu menyalahkan perempuan. Bentuk KDRT yangdilakukan suami terhadap anggota keluarganya dalam bentuk : 1) Kekerasanfisik ; 2) Kekerasan psikis, 3).Kekerasan seksual, 4). Penelantaran rumahtangga yang terjadi dalam lingkup rumah tangganya, Fikih Islam melarangsegala bentuk kekerasan tersebut. Hanya saja, Ada perbedaan antaraUU No. 23 Tahun 2004, dengan hukum Islam yang mana dalam fikihIslam dibolehkan memukul istri yang nusyuz dengan syarat-syarattertentu. Pembolehan memukul ini harus memenuhi syarat dan kaidahyang ditentukan oleh ulama fikih. Dari sisi yuridis alternatif pemecahanmasalah KDRT ditempuh melalui hukum privat, publik maupun administratif.Kata Kunci : KDRT, Hukum Positif dan hukum Islam

MENJADIKAN PESANTREN SEBAGAI PUSAT PERADABAN MUSLIM DI INDONESIA

Millah: Jurnal Studi Agama Vol. XI, No. 1, Agustus 2011 Pesantren dalam Dinamika Islam Indonesia
Publisher : Program Magister Ilmu Agama Islam, Universitas Islam Indonesia

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Abstract

This article discusses three main problems are related to the preparation of boarding schools (pesantren) to become the center of Muslim civilization in the global era. Firstly, it is releated to the existence of pesantren in Indonesia. Secondly, deals with opportunities and challenges of pesantren to become center of civilization in the global era. Thirdly, these things must be prepared by pesantren to be the center of Muslim civilization. Study in this article is the normative descriptive analysis which is result of in-depth review of the literature related with pesantren and civilization.  Historical facts show and proved that until now pesantren can still exist as a place of education for society and survived, even able to keep rhythm of turnaround of time. There is tremendous opportunity to make pesantren as a center of Muslim Civilization in Indonesia in the global era. However, there are many challenges too. Therefore, it is necessary preparations of the pesantren to capture these opportunities and overcome all challenges. These Preparations are, laid of core or foundation of civilizations, development paradigm of thinking civitas of pesantren, mastery and development of modern science and development of higher education institution of pesantren (mahad Aly).

Peneraan Antibodi Serum Mencit (Mus musculus) Sebelum dan Setelah Imunisasi dengan Sediaan Antifertilitas Zona Pelusida-3 Kambing

Media Kedokteran Hewan Vol 21, No 3 (2005): Media Kedokteran Hewan
Publisher : Media Kedokteran Hewan

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Abstract

The aim of the study was to determine the changing of optical density of antibody of mice (Mus musculus) immunized with 40 μg of fraction 3 of goat zona pellucida (gZP3) as an anti fertility agent.  Each of the mice injected three times, once as the first immunization and twice as booster in 14 days interval respectively. The first immunization, gZP3 suspension was diluted with Complete Freund’s Adjuvant (CFA), meanwhile the booster gZP3 suspension was diluted with Incomplete Freund’s Adjuvant (IFA) 1:1. Blood samples collection for  serum were carried out four times, i.e. before first immunization, before first booster, before second booster and after second booster. The measurement of antibody mice in serum using Elisa technique indicated the increasing (p<0.05) of optical density serum from after the first immunization until before the second booster injection. It could be concluded that gZP3 was effective as an immuno contraceptive substance. Key Words : fraction-3 of goat zona pellucida, pregnancy rate, litter size.