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EFFECT OF COMBINED THERAPY USING CHLOROQUINE AND VITAMIN C TO THE PERITONEAL MACROPHAGE FUNCTION IN BALB/C STRAIN MICE INFECTED BY Plasmodium berghei Fitri, Loeki Enggar; Suhendro, Wongso; Murwani, Sri; Muliartha, I Ketut Gede; Ali, Mulyohadi
Jurnal Kedokteran Brawijaya Vol 19, No 3 (2003)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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Abstract

In acute infection, malaria will induce cellular immunity by activating T lymphocytes and macrophages cells. This induction indirectly triggers free radicals production in order to eliminate the parasites from red blood cells, however high concentration of thismolecules can cause vital tissue pathological changes on host. In late phase of malarial infection, there are immunosupression on macrophages activity including antigen presenting and secretion of immunoregulated mediator. It has been anticipated, vitamin C as antioxidant would diminish the side effect of thesefree radicals during malarial infection and increase the immunity. To see the effect of combination chloroquin and vitamin C in hastening the recuperative process by decreasing parasitemia and increasing the phagocytosis function of macrofages during Plasmodium berghei infection. This study has been carried out using 3 groups of BALB/c mice, all group were inoculated with 1x107Plasmodium berghei infected red-blood cells. No drug was given on control group (IK). In experimental group we introduced an oral therapy ofchloroquin for 5 days in 1.4 mg/cc dosage and vitamin C for 7 days in 0.2 mg/cc dosages concurrently with a Plasmodium berghei inoculation (IKC). One group was only given chloroquin at the same dosage and no drug was given at the control group (IK).
IDENTIIFKASI PROTEIN IMUNOGENIK CHLAMYDIA PNEUMONIAE TERHADAP SERUM PENDERITA INFARK MIOARD AKUT Murwani, Sri; Hidayati, Dwi Yuni Nur
Jurnal Kedokteran Brawijaya Vol 23, No 2 (2007)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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Abstract

Chlamydia pneumoniae is human respiratory tract pathogen and recently investigated as pathogen causingatherosclerosis and acute myocardial infarction (AMI). This research was carrier out to detect proteinpattern of C. pneumoniae, and to study it relation to AMI throughdetection of immunogenic protein. Design research  was laboratory observational and analyzed descriptively. The subject was C. pneumoniae. Protein pattern the bacteria was detected by electrophoresis method, and to detect the immunogenic protein was done immunoblotting. Serum was obtained from AMI patients in Saiful Anwar and Lavallette hospitals.  The result showed, protein pattern C. pneumoniae wasprotein with molecular weight 117, 107, 97, 91, 86, 61, 58, 52, 46, 44, 34, 23, 19, 9, 5, 4 kDa. Immunogenic proteins vary between AMI patients was 117, 107, 86, 61, 58, 52, 46, 44,  34 kDa. Non immunogenic proteins were 97, 91, 23, 19, 9, 5 and 4 kDa. Protein 61 kDa react to all of patient’s serum. It was concluded, C. pneumoniae have protein fractions 117, 107, 97, 91, 86, 61, 58, 52, 46, 44, 34, 23, 19, 9, 5, and 4kDa. Immunogenic proteins vary between AMI patients was 117, 107, 86, 61, 58, 52, 46, 44, 34 kDa, and 61 kDa was the  immunodominant protein. The result proved C. pneumoniae as causative agent of atherosclerosis and acute myocardial infarction, in Indonesia particularly. Key words:C. pneumoniae, immunogenic, AMI
DIET ATEROGENIK PADA TIKUS PUTIH (Rattus novergicus strain Wistar) SEBAGAI MODEL HEWAN ATEROSKLEROSIS Murwani, Sri; Ali, Mulyohadi; Muliartha, Ketut
Jurnal Kedokteran Brawijaya Vol 22, No 1 (2006)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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Abstract

Atherosclerosis is still a major health problem, because 20% of death in the world is caused by atherosclerosis diseases like stroke and myocardial infarct. One of animal models that was successful in atherosclerosis research was New Zealand white rabbit. The purpose of this preliminary research was to determine the atherogenic diet ofwhite rats (Rattus novergicus strain Wistar) as animal model of atherosclerosis especially to find fixed composition and time of the atherogenic diet taken.This research used male; 2 months age, 150-200 grams body weight of white rats Rattus novergicus strain Wistar. The rats were divided into5 groups in equal number, 4 rats respectively. Theywere a negative control group without diet treatment and 4 groups as treatment groups which were given hipercholesterol diet. Atherogenic diet composed of PAR-S, wheat flour, cholesterol, cholic acid, pork oil and water. Data were obtained by measuring of blood cholesterol level and foam cell formation.
DETECTION PROTECTIVE ANTIGEN WITH MONOCLONAL ANTIBODY OF PASTEURELLA MULTOCIDA DOMPU ISOLATE Murwani, Sri; Asmara, Widya
Jurnal Sain Veteriner Vol 16, No 2 (1999)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8599

Abstract

Monoclonal antibodies can be used as specific probes for molecular structure such as drugs, hormones, receptor for hormones, or any other biologycally derived or biology-cally active materials. The potential usefulness of mono­clonal antibodies is in almost every field of biology and medicine.The study was carried out to produced monoclonal anti­bodies against outer membrane protein (OMP) Pasteurella multocida isolat Dompu, which can be used to isolate imunogenic OMP fractions.Three monoclonal antibodies which specifically react with OMP fraction of Pasteurella multocida isolat Dompu were obtained previously. Those monoclonal antibodies were pro­duced in Balb/c mouse. The antibodies production were measured by ELISA and were purified by affinity chromatograPhy.The monoclonal antibody were injected intraperitoneally into group of five rats. Two additional groups of five rats were injected with immune mouse serum and normal mouse serum, which served as a positive and negative controls respectively. All rats were challenged with 1 ml lethal dose of Pasteurella multocida 24 hours after the antibody injec­tion.The outer membrane protein was separated 12,5% SDS-PAGE. The region of gel containing the appropiate antigens (PI, P2, P3) were excised. Protein antigen was mixed with incomplete Freund's adjuvant (IFA) and injected intramuscu-lary into group of five rats at dose 0,2 ml per mouse. Two additional groups were injected with gel in IFA and OMP and IFA as a positive and negative controls. Each treated mouse and control was immunized four times, at weekly interval. Two weeks after final immunization rat were bled and their antibodies productions were measured with ELISA. All mice were then challenged with lethal dose of Pasteurella multo­cida.The result indicated that eventhough immungenic the PI, P2, P3 OMP fraction individually were not able to induce protective antibody. 
MONOCLONAL ANTIBODIES AGAINST OUTER MEMBRANE PROTEIN PASTEURELLA MULTOCIDA STRAIN DOMPU Murwani, Sri; Asmara, Widya
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8618

Abstract

Monoclonal antibodies were produced against outer membrane protein of Pasteurella multocida strain Dompu. The bacterial isolate was obtained from [he bacteria! collection held at Microbiology Laboratory, Faculty of Veterinary Medicine, Gadjah Mada University. Irs purity and patogenicily has beeen tested.Killed PatTeurella multocida were used ro immunized 7 female, 8-10 week old of Balb/ c mice. Cell fusion between limphocytes and myeloma cells (2:1) were carried out by PEG stimulation and bybrids were cultured in  HAT medium.The OMP were prepared from sonicated organism using centrifugallon in the presense of sarcosyL These antigens were than used to select antibody producing hvbridomas ELISA and cloning of hybridomas were done by serial dilution method.Four hybridomas clones were obtained, till producing monoclonal antibodies which reacted specifically against OMP Pasietirelta multacida stain Dompu.
DIET ATEROGENIK PADA TIKUS PUTIH (Rattus novergicus strain Wistar) SEBAGAI MODEL HEWAN ATEROSKLEROSIS Murwani, Sri; Ali, Mulyohadi; Muliartha, Ketut
Jurnal Kedokteran Brawijaya Vol 22, No 1 (2006)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1301.176 KB) | DOI: 10.21776/ub.jkb.2006.022.01.2

Abstract

Atherosclerosis is still a major health problem, because 20% of death in the world is caused by atherosclerosis diseases like stroke and myocardial infarct. One of animal models that was successful in atherosclerosis research was New Zealand white rabbit. The purpose of this preliminary research was to determine the atherogenic diet ofwhite rats (Rattus novergicus strain Wistar) as animal model of atherosclerosis especially to find fixed composition and time of the atherogenic diet taken.This research used male; 2 months age, 150-200 grams body weight of white rats Rattus novergicus strain Wistar. The rats were divided into5 groups in equal number, 4 rats respectively. Theywere a negative control group without diet treatment and 4 groups as treatment groups which were given hipercholesterol diet. Atherogenic diet composed of PAR-S, wheat flour, cholesterol, cholic acid, pork oil and water. Data were obtained by measuring of blood cholesterol level and foam cell formation.
IDENTIIFKASI PROTEIN IMUNOGENIK CHLAMYDIA PNEUMONIAE TERHADAP SERUM PENDERITA INFARK MIOARD AKUT Murwani, Sri; Hidayati, Dwi Yuni Nur
Jurnal Kedokteran Brawijaya Vol 23, No 2 (2007)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (661.466 KB) | DOI: 10.21776/ub.jkb.2007.023.02.6

Abstract

Chlamydia pneumoniae is human respiratory tract pathogen and recently investigated as pathogen causingatherosclerosis and acute myocardial infarction (AMI). This research was carrier out to detect proteinpattern of C.?pneumoniae, and to study it relation to AMI throughdetection of immunogenic protein. Design research ?was laboratory?observational and analyzed descriptively. The subject was C. pneumoniae. Protein pattern the bacteria was detected by?electrophoresis method, and to detect the immunogenic protein was done immunoblotting. Serum was obtained from AMI?patients in Saiful Anwar and Lavallette hospitals. ?The result showed, protein pattern C. pneumoniae wasprotein with?molecular weight 117, 107, 97, 91, 86, 61, 58, 52, 46, 44, 34, 23, 19, 9, 5, 4 kDa. Immunogenic proteins vary between?AMI patients was 117, 107, 86, 61, 58, 52, 46, 44, ?34 kDa. Non immunogenic proteins were 97, 91, 23, 19, 9, 5 and 4?kDa. Protein 61 kDa react to all of patient?s serum. It was concluded, C. pneumoniae have protein fractions 117, 107, 97,?91, 86, 61, 58, 52, 46, 44, 34, 23, 19, 9, 5, and 4kDa. Immunogenic proteins vary between AMI patients was 117, 107,?86, 61, 58, 52, 46, 44, 34 kDa, and 61 kDa was the ?immunodominant protein. The result proved C. pneumoniae as?causative agent of atherosclerosis and acute myocardial infarction, in Indonesia particularly.?Key words:C. pneumoniae, immunogenic, AMI
Detection of Staphylococcus aureus Biofilm from Subclinical Mastitis Milk Lesmana, Muhamad Arfan; Qosimah, Dahliatul; Murwani, Sri
Veterinary Biomedical and Clinical Journal Vol 1, No 1 (2019)
Publisher : Veterinary Biomedical and Clinical Journal

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Abstract

One of S.aureus's virulence factors is biofilm formation. When biofilms are formed, the bacteria will undergo phenotypic changes that require higher concentrations of antibiotics to inhibit proliferation. Phenotypic changes will lead to increase the production of extracellular matrix and multilayered colonies as well as decrease of metabolic rates, multiplication and polymicrobial colonization resulting in recurrent infection in the host and difficulty being treated with antibiotics due to resistance. The aim of this research was to know the formation of bacterial biofim by slime and quantitative by microplate titer method. The research method was qualitative descriptive using 27 samples of Staphylococcus aureus  with characterized from mastitis infected milk. The bacteria were grown on CRA (Congo Red Agar) media to observe the slime biofilm through bacteria black colony followed by MicrotiterPlate  method  with 570nm wave lenght. The results showed that 27 samples of Staphylococcus aureus which positive to form slime biofilm were 10 samples and continued to microtiter plate showed 3 positive samples of biofilm. The conclusions of this study, Staphylococcus aureus in subclinical mastitis milk samples were positive to form biofilms. 
PEMBERIAN PROTEIN ADHESIN 38-KILODALTON MYCOBACTERIUM TUBERCULOSIS PERORAL MENINGKATKAN JUMLAH MAKROFAG DAN LIMFOSIT USUS MENCIT BALB/c Triliana, Rahma; Kartosen, Ade A; Puspitasari, Dianika P; Murwani, Sri; Santoso, Sanarto; Arthamin, Maimun Z
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 2 (2011)
Publisher : PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i2.1015

Abstract

Tuberculosis (TB), caused by Mycobacterium tuberculosis (M.tb), is one of the world health problems. Oral vaccination of M.tb hasa potential to reduce the risk and complication of TB. The 38-kDa adhesin protein as one of oral TB vaccine candidates has not beenproven. This study is aimed to determine M.tb 38-kDa adhesin protein effect on macrophage and lymphocyte numbers in mice intestineafter an oral administration. BALB/c mice (n=20), age 6?8 weeks, and were divided into 4 groups: control (K), adjuvant (A), 38-kDa100?g adhesin protein (P), and combination of 100?g 38-kDa adhesin protein with adjuvant (PA). An oral administration was givenat the beginning with 2 boosters every 4 weeks. After 3 days of the second booster, the mice were killed and the intestine was taken andstained with haematoxylin eosin (HE) to measure its macrophages and lymphocytes number. The mean ?2SD were 18.4 (3.71) and6.09 (0.34), 23.0 (7.78) and 8.86 (1.19), 42.2 (13.63) and 23.49 (3.91), 95.4 (30.11), and 53.57 (13.79) respectively for K, A, Pand PA group. The statistical test showed a significant difference among each group revealing the role of M.tb 38-kDa adhesin proteinas immunogenic inducing cellular immunity in intestine. In this study, so far it was found that the oral administration of M. tb 38-kDaadhesin protein has an ability to increase macrophage and lymphocyte numbers in the mice intestinal BALB/c.
EFFECT OF COMBINED THERAPY USING CHLOROQUINE AND VITAMIN C TO THE PERITONEAL MACROPHAGE FUNCTION IN BALB/C STRAIN MICE INFECTED BY Plasmodium berghei Fitri, Loeki Enggar; Suhendro, Wongso; Murwani, Sri; Muliartha, I Ketut Gede; Ali, Mulyohadi
Jurnal Kedokteran Brawijaya Vol 19, No 3 (2003)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1633.38 KB) | DOI: 10.21776/ub.jkb.2003.019.03.2

Abstract

In acute infection, malaria will induce cellular immunity by activating T lymphocytes and macrophages cells. This induction indirectly?triggers free radicals production in order to eliminate the parasites from red blood cells, however high concentration of thismolecules?can cause vital tissue pathological changes on host. In late phase of malarial infection, there are immunosupression on?macrophages activity including antigen presenting and secretion of immunoregulated mediator. It has been anticipated, vitamin C as?antioxidant would diminish the side effect of thesefree radicals during malarial infection and increase the immunity. To see the effect?of combination chloroquin and vitamin C in hastening the recuperative process by decreasing parasitemia and increasing the?phagocytosis function of macrofages during Plasmodium berghei infection. This study has been carried out using 3 groups of BALB/c?mice, all group were inoculated with 1x107Plasmodium berghei infected red-blood cells. No drug was given on control group (IK). In?experimental group we introduced an oral therapy ofchloroquin for 5 days in 1.4 mg/cc dosage and vitamin C for 7 days in 0.2 mg/cc?dosages concurrently with a Plasmodium berghei inoculation (IKC). One group was only given chloroquin at the same dosage and no?drug was given at the control group (IK).