ENDANG MURNIATI
Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti Kampus IPB Darmaga, Bogor 16680, Indonesia

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The Effect of Ascorbic Acid Treatment on Viability and Vigor Maize (Zea mays L.) Seedling under Drought Stress HAMAMA, HAMIDAH; MURNIATI, ENDANG
HAYATI Journal of Biosciences Vol 17, No 3 (2010): September 2010
Publisher : Bogor Agricultural University, Indonesia

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Abstract

This study was conducted in the laboratory and the field to examine the effects of ascorbic acid treatment on germination and seedling growth under drought stress. The laboratory works consisted of two experiments and were designed to determine the critical osmotic potential of maize and to determine the optimum ascorbic acid concentration. The field study was designed to examine the effects of soaking seed in ascorbic acid on seedling growth under drought stress. Drought condition was simulated by PEG-6000 and regulation of water treatment. During the first experiment, interactions of both osmotic potential and varieties were significant at all variables. Germination percentage and speed of germination were significantly decreased by increasing of osmotic potential. The second experiment showed that interactions of both factors were significant at all variables except vigor index, the length of shoot, primary, and seminal root. The results showed that the ascorbic acid treatment improved the germination percentage, the speed of germination and the vigor index compared with the control, besides the increase in length of shoot, primary and seminal root and number of seminal root. However, the best result was showed by 55 mM ascorbic acid. The result of field experiment showed that interactions were not always significant and 55 mM ascorbic acid treatment increased the seedling height, the number of leaves and leaf area but it had no effect on the water deficit and the root length.
Pengaruh Jenis Media Perkecambahan dan Perlakuan Pra Perkecambahan terhadap Viabilitas Benih Mengkudu (Morinda citrifolia L.) dan Hubungannya dengan Sifat Dormansi Benih Murniati, Endang; Suminar, Marlia
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 34, No 2 (2006): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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The experiment was conducted at Leuwikopo Lath House and Seed Science and Technology Laboratory Department of Agronomy, Bogor Agricultural University, Darmaga, Bogor, from March to June 2004. The objective of this research was to study the effects of germination substrate and pre germination treatment on the noni seed viability in relation to the seed dormancy. The research was arranged in the Randomized Complete Block Design with two factors. The first factor was germination substrates, consisted of sand, paddy charcoal and soil mixed with compost (1:1). The second factor was pre germination treatments, consisted of control, scarification, KNO3 1%, KNO3 2%, warm water (470C), combination of scarification and KNO3 1%, combination of scarification and KNO3 2%, combination of scarification and warm water (470C). This result showed that soil mixed with compost (1:1) was the best substrate for noni seed germination. Pre germination treatments as well as interaction between germination substrate and pre germination treatment had no significant effect on noni seed viability. Enforced dormancy occured on seeds which was germinated on paddy charcoal substrate.   Key words : Morinda citrifolia seed, pre germination, paddy charcoal, enforced dormancy
Pengaruh Pemeraman Buah dan Periode Simpan terhadap Viabilitas Benih Pepaya (Carica papaya L.) Murniati, Endang; Sari, Maryati; Fatimah, Ema
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 36, No 2 (2008): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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The objective of  this experiment was to study  the influence of post-harvest storage and storage period to seed viability. The research was conducted in the Laboratory of Seed Science and Technology and the green house at Leuwikopo IPB Darmaga, from February until August 2007. The experiment used split-plot design with three replications. The main plot was seven periods of storage: 0, 3, 6, 9, 12, 15, and 18 weeks. The sub plot was the period of post-harvest maturation storage (use the fruits with 30-40% yellow coloured): 0, 2, 4, 6 days post-harvest storage, and control fruits that ripe on the tree (with 80-90% yellow coloured). The control fruits have the best seed viability and vigor. Viability of  seed from fruits with 0, 2 and 6 days post-harvest storage was significantly less than that of control. Seed from four days post-harvest storage have same viability as control. Post-harvest storage can improve physiological seed quality on the seed that must be harvested before the time of physiological maturity, especially four days post-harvest storage.    Key words:  Papaya, seed, viability, post-harvest maturation storage, storage period
Pengaruh Perlakuan Deoperkulasi Benih dan Media Perkecambahan untuk Meningkatkan Viabilitas Benih Aren (Arenga pinnata (Wurmb.) Merr.) Rofik, Aenur; Murniati, Endang
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 36, No 1 (2008): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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The research was aimed at studying the effects of seed deoperculation treatment and germination substrate to enhance viability of sugar palm seed. This research was conducted from February until August 2006 at Seed Science and Technology Laboratory and Turfgrass area. Randomized block design with 2 factors was used in this research. The first factor consisted of five treatments namely: control, without seed treatment (P0), deoperculation with sand paper precisely on the embryo position (PI), deoperculated seed and heating in the incubator at 40oC for 5 minutes (P2), deoperculated seed and soaking in the potassium nitrate (KNO3 0.5%) for 36 hours (P3) and deoperculated seed, soaking in the potassium nitrate for 36 hours and heating in the incubator at 40oC for 5 minutes (P4). The second factor substrate used for germination consisted of five types, i.e., sand (M1), soil and compost mixed, each in equal (1:1) portions by weight (w/w) (M2), saw dust (M3), cocopeat (M4) and paddy charcoal (M5). The result showed that seed deoperculation gave very significant effect to enhance seed viability. The interaction between seed treatment and substrate significantly influenced on potential growth, germination percentage, speed of germination, the length of embryonic axis and the length of root. The highest potential growth of 96.67% was obtained from deoperculated seed and heating in the incubator at 40oC for five minutes and germinated in sand, whereas highest germination percentage of 88.33% was reached by deoperculated seed and germinated in sand. Sand (M1), cocopeat (M4) and paddy charcoal (M5) were suitable for germination substrate of sugar palm seed.   Key words:  Sugar palm seed, seed viability, deoperculation, cocopeat, paddy charcoal.
Pengaruh Skarifikasi dan Media Tumbuh Terhadap Viabilitas Benih dan Vigor Kecambah Aren Saleh, Muhammad Salim; Adelina, Enny; Murniati, Endang; Budiarti, Tati
Jurnal Ilmu Pertanian Indonesia Vol 13, No 1 (2008): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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The research aims to find out the effect of seed scarification and germination substrat to seed viability and seedling vigor of aren. The Experiment applied a group random design (RAK) with factorial style consiting of two factors. The first factor are scarification treatment:S0=without scarification, S1=scarification+trcatment 40 oC, S2=scarification+KN03 0.5%, soaked for 36 hours+tempcrature 40oC, the second factor arc germination substrat: M0=sand, M1=rice coal, M2=cocopith, M3=soil from palm tree+organic materil (1:1), M4=M3+fertilizer NPK (1 g per kg media). The highest seed germination was found in the scarification+KN03 0.5%, soaked for 36 hours+ temperature 40 oC which growing on media of palm oil soil+organic fertilizer (1:1)+fertilizer NPK (1g per kg media) that 83.33-86.67% and germinating speed 0.85-1.04% ctmal. There treatment also effected on seedling vigor normal vigours germination indicated by dry content weight and hypotctic vigor index. Keywords: aren, scarification, germination substrat
Karakterisasi 10 genotipe jarak pagar (Jatropha curcas L.) lokal Surahman, Memen; Murniati, Endang; ., Misnen
Jurnal Ilmu Pertanian Indonesia Vol 15, No 1 (2010): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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This study aimed to obtain genotypes of local jatropha has the potential to be developed through the characterization based on quantitative and qualitative characters. The experiment was conducted in August 2009-April 2010 took place at the experimental PT. Indocement, Citereup-Bogor. Materials used jatropha cuttings from 10 genotypes (Banten, Medan, Biak, IP-2P, Bengkulu, Sukabumi, Bali, Sulawesi, Bogor, and Lombok). The observed character is the character of quantitative and qualitative. The results showed 10 genotypes Jatropha tested has the color green of young stems and old stems green color gray. The color ofshoots is green, and coloor of old leaf is dark green.The color of leaf bones is purple and green, leaf textureis coarse, and leaves bones clearly visible. Genotype that has hermaphrodite flowers are Banten, Biak, Bengkulu, and Sukabumi. The best genotype was Banten with leaf number 74, branch height 48 em, number of panicles 1 plant 12.2, the number of fruits I plant 110.5, wet seed weight I plant 288.7 g, and dry seed weight I plant 193.8 g. Variables wet seed weight I plant is a selection of characters that can be used to increase the yield of dry seed weight 1 plant. Another character that indirectly affect significantly positive effect on dry seed weight of stem diameter, leaf number, number of fruits per panicle, and number of fruit I plant. Selection on vegetative characters can not be used as selection criteria.Keywords: Jatropha curcas, the character of quantitative, qualitative character, selection.
KOMPATIBILITAS BATANG BAWAH NANGKA TAHAN KERING DENGAN ENTRIS NANGKA ASAL SULAWESI TENGAH DENGAN CARA SAMBUNG PUCUK Tambing, Yohanis; Adelina, Enny; Budiarti, Tati; Murniati, Endang
AGROLAND Vol 15, No 2 (2008)
Publisher : AGROLAND

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The research on vegetative breeding of jack fruit has been conducted through grafting method, aiming at finding out the compatibility of lower cultivar resisting to drought stress with the jack fruit entries of pre-eminent varieties originally from central Sulawesi. Generally, the results of the research indicated that all entries except those grafted with the root stock BK-3 and TL-5 could only survive for 2 weeks leaving only their dead scions.  The latter appeared to be compatible with the Palupi entries as they were still able to live and grew until this research ended
PENGARUH SKARIFIKASI DAN MEDIA TUMBUH TERHADAP VIABILITAS BENIH DAN VIGOR KECAMBAH AREN Saleh, Muhammad Salim; Adelina, Enny; Murniati, Endang; Budiarti, Tati
AGROLAND Vol 15, No 3 (2008)
Publisher : AGROLAND

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Abstract

The research aim was to find out the effect of seed scarification and germination substrate upon seed viability and seedling vigor of aren.  The research applied a randomized block design with factorial pattern consisting of two factors.  The first factor was scarification treatment: without scarification, scarification at 400C, scarification at 400C and soaked in 0.5% KNO3 solution for 36 hours.  The second factor was germination substrate:  sand, rice hull coal, cocopith, mixture of soil taken from natural palm growth area and organic material at a ratio 1:1, and mixture of 1 g NPK fertilizer with 1 kg soil taken from palm tree forest area and organic material media.  The greatest seed germination of 83.33–86.67% with germinating speed of 0.85-1.04 %etmal was found in the scarification at 400C soaking in 0.5% KNO3 solution for 36 hours and soil organic and matter treatment. This treatment also resulted in normal vigorous seed indicated by higher dry content weight and hypothetic vigor index than any other treatments.
PENGARUH KAPUR TOHOR UNTUK EKSTRAKSI BENIH TERHADAP VlABILITAS BEIH MANGGIS (Garcinia mangostana L Murniati, Endang; Rostiati, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 27, No 1 (1999): Buletin Agronomi
Publisher : Bogor Agricultural University

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The objectives of this experiment were to evaluate the possibility of using quick lime for extraction of mangosteen seed and the effects on its viability; The experiment was done in Seed Science and Technology Laboratory, Greenhouse, Bogor Agriculture University from November 1997 until February 1998. The materials were taken from smallholder plantation in Jasinga, Bogor. The treatments were arranged factorial in a group randomized block design with two factors. The first factor was 6 levels of quick lime concentration (K) : 0 g/l, 10 g/l, 15 g/l, 20 g/l, 25 g/l, 30 g/l and the second factor was 4 levels of soak periods: 0 minute, 10 minutes, 20 minutes, 30 minutes. The experiments had 24 treatments combination with 4 replicates so there were 72 experiment units. The data show that seed extraction using quick lime gave statistically significant different results compared with seed extraction without using quick lime and so the soak periods compared with no soak. Interaction between 20 g/l quick lime concentration and 30 minutes soak period gave the best seed emergence (96 %). 20 g/l quick lime concentration save higher vigour than other concentration as measured from its speed of germination, spontaneous growth, and root length. The soak periods increased seed moisture content 30 minutes soak period resulted higher viability and vigour than other soak periods as measured from its normal seed dry weigh, spontaneous growth, epycotyl length and root length.
Pengaruh Sarcotesta dan Kadar Air Benih terhadap Kandungan Total Fenol dan Daya Simpan Benih Pepaya (Carica papaya L.) Sari, Maryati; Suhartanto, M. R.; Murniati, Endang
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 35, No 1 (2007): Jurnal Agronomi Indonesia
Publisher : Bogor Agricultural University

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There are phenolic compounds in sarcotesta of papaya seed which can act either as inhibitor or as antioxidant.  The objective of this experiment was to study the effect of sarcotesta and seed moisture content on total phenolic content and seed longevity of papaya seed.  The experiment was conducted in November 2004 - May 2005, at Bogor Agicultural University, by using papaya seed (IPB-1) harvested from Center for Tropical Fruit Studies orchard in Bogor. In this study, seeds were dried in the absence and presence of sarcotesta until 11-12% and 6-7% moisture content (mc).  After drying, seeds were packed in sealed plastic bag and stored in ambient room until 0, 3, 6, 9, 12 and 15 weeks. Seed viability and total phenolic compound were evaluated every 3 weeks.  Total phenolic content of seed with sarcotesta was higher (> 327mg/100g dry weight) than the seed without sarcotesta (