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Pemetaan Gen Resistensi Lapang terhadap Penyakit Hawar Daun Kentang ~ pada Populasi Fl Solanum tuberosum (SH2988) x Solanum microdontum (MCD167) Tutupary, J. M.; Wattimena, Gustav A.; Aswidinnoor, Hajrial; Muladno, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 32, No 3 (2004): Buletin Agronomi
Publisher : Bogor Agricultural University

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Abstract

Potatoes can be severely affected by late blight, a fungal disease caused by Phvtophthora infestans. which can destroy the foliage and tubers of the crop. Initial attempts to control late blight in potato deployed resistance genes (R genes) derived from the hexaploid Solanum demissum (RI - Rll). However, their immunity reaction can be easily overcome by the development of new virulent factors. It is currently accepted that the most effective solution against late blight involve the use offield-resistant varieties as part of an integrated pest management strategy. Field resistance to Phvtophthora i!!festans were examined in a diploid segregating potato population. The population produced crosses between Solanum microdontum genotype (MCDI67) and ~ tuberosum genotype (SH2988) have been assessed for resistance to f.. infestans (late blight) in the field and prol1ed to segregate for f.. infestans resistance. A genetic linkage map of this population was constructed by using PCR-based CAPS markers. The result showed that one QTL on foliage resistance to late blight were linked with marker GP 180 on chromosome 4 of potato in coupling phase. Key words: CAPS markers, Field resistance, Late blight, Phvtophthora infestans. potato, QTL
KARAKTERISASI GEN -LAKTOGLOBULIN DAN HUBUNGANNYA DENGAN SIFAT PRODUKSI SUSU PADA SAPI HISSAR Maskur, ,; Sumantri, Cece; Muladno, ,
Zuriat Vol 16, No 2 (2005)
Publisher : Zuriat

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Abstract

Karakteristik gen-laktoglobulin dan hubungannya dengan sifat produksi susu pada 42 sapi Hissar dianalisis menggunakan teknik PCR-RFLP. Fragmen DNA-produk CPR dan hasil pemotongan menggunakan enzim restriksi Hae III dipisahkan menggunakan elektroforesis gel poliarkrilamid (PAGE) kemudian dideteksi dengan pewarnaan perak. Jumlah allel gen-laktoglobulin yang terdeteksi ada dua macam yaitu alel-A dan B dengan frekuensi masing-masing 0.19 dan 0.81. Uji signifikansi Chi-Square menunjukkan bahwa distribusi genotip gen-laktoglobulin dalam populasi sapi Hissar di Moyo Hilir Sumbawa-NTB berada dalam ketidakseimbangan dimana frekuensi genotip BB 69.05%; AB 23.81% dan AA 7.14%. Tingkat produksi susu memiliki korelasi yang kuat dengan genotip gen -laktoglobulin. Rata-rata produksi susu sapi Hissar dengan genotip AA, AB dan BB berturut-turut: 4.30 lt/hr; 3.11 lt/hr, dan 2.17 lt/hr.
Pemetaan Gen Resistensi Lapang terhadap Penyakit Hawar Daun Kentang ~ pada Populasi Fl Solanum tuberosum (SH2988) x Solanum microdontum (MCD167) Tutupary, J. M.; Wattimena, Gustav A.; Aswidinnoor, Hajrial; Muladno, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 32, No 3 (2004): Buletin Agronomi
Publisher : Indonesia Society of Agronomy

Show Abstract | Original Source | Check in Google Scholar

Abstract

Potatoes can be severely affected by late blight, a fungal disease caused by Phvtophthora infestans. which can destroy the foliage and tubers of the crop. Initial attempts to control late blight in potato deployed resistance genes (R genes) derived from the hexaploid Solanum demissum (RI - Rll). However, their immunity reaction can be easily overcome by the development of new virulent factors. It is currently accepted that the most effective solution against late blight involve the use offield-resistant varieties as part of an integrated pest management strategy. Field resistance to Phvtophthora i!!festans were examined in a diploid segregating potato population. The population produced crosses between Solanum microdontum genotype (MCDI67) and ~ tuberosum genotype (SH2988) have been assessed for resistance to f.. infestans (late blight) in the field and prol1ed to segregate for f.. infestans resistance. A genetic linkage map of this population was constructed by using PCR-based CAPS markers. The result showed that one QTL on foliage resistance to late blight were linked with marker GP 180 on chromosome 4 of potato in coupling phase. Key words: CAPS markers, Field resistance, Late blight, Phvtophthora infestans. potato, QTL