A. Mujnisa
Fakultas Peternakan Universitas Hasanuddin

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The Resistance of Lactic Acid Bacteria Isolated From the Faeces of Broiler chicken towards the Conditions of the Broiler Digestive Tract Mujnisa, A.; Rotib, Laily A.; Djide, Natsir; Natsir, Asmuddin
JURNAL ILMU DAN TEKNOLOGI PETERNAKAN Vol 2, No 3 (2013)
Publisher : Fakultas Peternakan Universitas Hasanuddin

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Abstract

This research was conducted to determine the resistance degree of lactic acid bacteria (LAB) isolated from the faeces of broilers towards various intestinal conditions, in order to select a lactic acid bacteria to be used as prospective probiotic. Ten types of LAB bacteria (M1, M2, M3, M4, M5, M7, M8, M23 ,M26, and M28) isolated from the faeces of broiler chicken were subjected to pH2, pH3, and pH4; to 3% bile salt and to the temperatures of 30oC, 37oC and 41oC. The results of the research showed that all LAB isolates experienced the highest colony reductions at pH2, which was around 3.2 - 5.5 log units/ml. The reduction of colony numbers at pH3 was approximately 1.8-3.1 log unit/ml, and the reduction at pH4 was around 1.0 – 2.5 log unit/ml. All the LAB isolates experienced a decrease of 0.5 log unit/ml to 1.6 log unit/ml in colony numbers after bile salt exposure. The reduction of colony numbers of ten LAB isolates at the temperatures of 30oC, 37oC and 41oC was 0.6 - 1.4 log unit/ml, 0.1 – 0.5 log unit/ml and 0.7 – 1.4 log unit/ml, respectively. None of the ten isolates posses all of the expected characteristic, however based on its more superior ability to block the pathogenic microorganism as well as its ability to survive in simulated gastro intestinal tract conditions, M1 was selected as prospective probiotic.
The Withdrawal Time of The Antibiotic of Zinc Bacitracin towards Its Residue in Broiler Andy, Andy; Agustina, L.; Mujnisa, A.
Jurnal Ilmu dan Teknologi Peternakan Vol 4, No 3 (2016)
Publisher : Fakultas Peternakan, Universitas Hasanuddin, Makassar

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Abstract

Genetic development of broiler is growing rapidly. Nowadays, broiler is slaughtered at the ages of 29 days rather than 35 days. Rapid growth is usually coupled by an immune deficiencies of broiler, therefore such feed additives as antibiotics is necessary to promote growth and improve endurance of the broiler. The purpose of this research was to investigate the amount of residue of Zinc bacitracin antibiotics in the thigh meat, breast meat, liver, and kidney of the broilers; and to determine the withdrawal time between the discontinuation of the antibiotic of the Zinc bacitracin and the slaughter of the broiler to eliminate or to reduce that residue so that the product of the broiler was safe for human consumption. The research was conducted at Sekolah Tinggi Penyuluhan Pertanian (STPP) Gowa. The broilers used in this study was strain Cobb SR 707, aged 29 days (unsexed), and were obtained from the local broiler farm. The ration containing the antibiotics of the Zinc bacitracin was terminated at day 0, 3, 6, and 9 before slaughtering. The bacteria used to test the dilution sensitivity was Staphylococcus aureus (105-108 colony mL-1). The transmittance reading of the sample solution from the Spectrophotometer was inputted into standard curve generated by from pure antibiotics to determine the antibiotics residue in the samples. The results were then compared with the value of SNI Number: 01-6366-2000 of year 2000. The data were analyzed descriptively and with a non linier regression analysis. The results revealed that the level of the Zinc bacitracin residue decreased following the duration of the cessation of the Zinc bacitracin antibiotic treatment. The antibiotic residue in the sample was smaller than that set in the standards (SNI, 2000) so that the broiler products were safe for human consumption.
The Resistance of Lactic Acid Bacteria Isolated From the Faeces of Broiler chicken towards the Conditions of the Broiler Digestive Tract Mujnisa, A.; Rotib, L. A.; Djide, N.; Natsir, A.
Jurnal Ilmu dan Teknologi Peternakan Vol 2, No 3 (2013)
Publisher : Fakultas Peternakan, Universitas Hasanuddin, Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This research was conducted to determine the resistance degree of lactic acid bacteria (LAB) isolated from the faeces of broilers towards various intestinal conditions, in order to select a lactic acid bacteria to be used as prospective probiotic.  Ten types of LAB bacteria  (M1, M2, M3, M4, M5, M7, M8, M23 ,M26, and M28) isolated from the faeces of broiler chicken were subjected to pH2, pH3, and pH4; to 3% bile salt and to the temperatures of 30oC, 37oC and 41oC. The results of the research showed that all LAB isolates experienced the highest colony reductions at pH2, which was  around 3.2-5.5 log units/ml. The reduction of colony numbers at pH3  was approximately 1.8-3.1 log unit/ml, and the reduction at pH4 was around 1.0 – 2.5 log unit/ml. All the LAB isolates experienced a decrease of 0.5 log unit/ml to 1.6 log unit/ml  in colony numbers after bile salt exposure. The reduction of colony numbers  of ten LAB isolates at the temperatures of 30oC, 37oC and 41oC was 0.6 - 1.4 log unit/ml ; 0.1 – 0.5 log unit/ml and 0.7 – 1.4 log unit/ml, respectively. None of the ten  isolates posses all of the expected characteristic, however based on its more superior ability to block the pathogenic micro-organism as well as its ability to survive in simulated gastro intestinal tract conditions, M1 was selected as prospective probiotic.
The Withdrawal Time of The Antibiotic of Zinc Bacitracin towards Its Residue in Broiler Andy, Andy; Agustina, L.; Mujnisa, A.
Jurnal Ilmu dan Teknologi Peternakan Vol 4, No 3 (2016)
Publisher : Fakultas Peternakan, Universitas Hasanuddin, Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Genetic development of broiler is growing rapidly. Nowadays, broiler is slaughtered at the ages of 29 days rather than 35 days. Rapid growth is usually coupled by an immune deficiencies of broiler, therefore such feed additives as antibiotics is necessary to promote growth and improve endurance of the broiler. The purpose of this research was to investigate the amount of residue of Zinc bacitracin antibiotics in the thigh meat, breast meat, liver, and kidney of the broilers; and to determine the withdrawal time between the discontinuation of the antibiotic of the Zinc bacitracin and the slaughter of the broiler to eliminate or to reduce that residue so that the product of the broiler was safe for human consumption. The research was conducted at Sekolah Tinggi Penyuluhan Pertanian (STPP) Gowa. The broilers used in this study was strain Cobb SR 707, aged 29 days (unsexed), and were obtained from the local broiler farm. The ration containing the antibiotics of the Zinc bacitracin was terminated at day 0, 3, 6, and 9 before slaughtering. The bacteria used to test the dilution sensitivity was Staphylococcus aureus (105-108 colony mL-1). The transmittance reading of the sample solution from the Spectrophotometer was inputted into standard curve generated by from pure antibiotics to determine the antibiotics residue in the samples. The results were then compared with the value of SNI Number: 01-6366-2000 of year 2000. The data were analyzed descriptively and with a non linier regression analysis. The results revealed that the level of the Zinc bacitracin residue decreased following the duration of the cessation of the Zinc bacitracin antibiotic treatment. The antibiotic residue in the sample was smaller than that set in the standards (SNI, 2000) so that the broiler products were safe for human consumption.
The Resistance of Lactic Acid Bacteria Isolated From the Faeces of Broiler chicken towards the Conditions of the Broiler Digestive Tract Mujnisa, A.; Rotib, L. A.; Djide, N.; Natsir, A.
Jurnal Ilmu dan Teknologi Peternakan Vol 2, No 3 (2013)
Publisher : Fakultas Peternakan, Universitas Hasanuddin, Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This research was conducted to determine the resistance degree of lactic acid bacteria (LAB) isolated from the faeces of broilers towards various intestinal conditions, in order to select a lactic acid bacteria to be used as prospective probiotic.  Ten types of LAB bacteria  (M1, M2, M3, M4, M5, M7, M8, M23 ,M26, and M28) isolated from the faeces of broiler chicken were subjected to pH2, pH3, and pH4; to 3% bile salt and to the temperatures of 30oC, 37oC and 41oC. The results of the research showed that all LAB isolates experienced the highest colony reductions at pH2, which was  around 3.2-5.5 log units/ml. The reduction of colony numbers at pH3  was approximately 1.8-3.1 log unit/ml, and the reduction at pH4 was around 1.0 ? 2.5 log unit/ml. All the LAB isolates experienced a decrease of 0.5 log unit/ml to 1.6 log unit/ml  in colony numbers after bile salt exposure. The reduction of colony numbers  of ten LAB isolates at the temperatures of 30oC, 37oC and 41oC was 0.6 - 1.4 log unit/ml ; 0.1 ? 0.5 log unit/ml and 0.7 ? 1.4 log unit/ml, respectively. None of the ten  isolates posses all of the expected characteristic, however based on its more superior ability to block the pathogenic micro-organism as well as its ability to survive in simulated gastro intestinal tract conditions, M1 was selected as prospective probiotic.