. MUHTADI
Department of Fisheries, Faculty of Agriculture, Gadjah Mada University

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Selective Media for In Vitro Activity Evaluation of Bacterial Biocontrol Against Pathogenic Vibrio ISNANSETYO, ALIM; MUHTADI, .; ISTIQOMAH, INDAH; NITIMULYO, KAMISO HANDOYO; TRIYANTO, .
HAYATI Journal of Biosciences Vol 18, No 3 (2011): September 2011
Publisher : Bogor Agricultural University, Indonesia

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Abstract

In vitro activity test is a critical evaluation to screen the potential biocontrol agent. We developed a selective medium for quantitative in vitro activity evaluation of bacterial biocontrol agents against pathogenic Vibrio in aquaculture. Sensitivity test of bacterial biocontrol and Vibrio spp. to nine antibiotics showed that oxytetracycline inhibited the growth of Vibrio spp., but did not inhibit the growth of the bacterial biocontrol. This selective inhibition activity of oxytetracycline suggested this antibiotic might be supplemented to establish a selective medium. The MIC of oxytetracycline against V. harveyi, V. parahaemolyticus, and V. algosus were 120, 250, and 120 mg/ml, respectively. These concentration did not inhibit the growth of bacterial biocontrols. Therefore, oxytetracycline was supplemented at 250 µg/ml in Zobell agar medium. This medium was used as a selective medium to enumerate the density of bacterial biocontrol. In vitro activity test of bacterial biocontrol (RLP1) against V. parahaemolyticus showed that strain RLP1 at density of 104 and 106 cells/ml was able to kill V. parahaemolyticus during 6 h incubation. At lower density, 102 cells/ml, this bacterial biocontrol agent was able to kill the pathogenic Vibrio during 12 h incubation. This study discovered a selective medium for the bacterial biocontrol and Vibrio spp. and provided the results of its application in the evaluation of in vitro activity of a bacterial biocontrol agent against V. parahaemolyticus. The results also revealed that strain RLP1 is a potential bacterial biocontrol against vibriosis in marine aquaculture.
PENGEMBANGAN POTENSI EKSTRAK KULIT BUAH RAMBUTAN SEBAGAI BAHAN OBAT HERBAL ANTIHIPERKOLESTEROL Muhtadi, .; Haryoto, .; Sujono, Tanti Azizah; Indaryudha, Peni; Suhendi, Andi
Biomedika Vol 5, No 2 (2013): Biomedika Agustus 2013
Publisher : Universitas Muhamadiyah Surakarta

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Abstract

The rind of rambutan fruit (Nephelium lappaceum L.) has been reported to have a very strong antioxidant activity with IC50 value of 7.74 μg / mL. The fruit contains  avonoids and phenolic . This compounds are potentially as hypolipidemic effect. This study aims to determine the hypolipidemic effect of 96% of ethanolic extract of rind of rambutan fruit in wistar strain male rats. This research method is the pre- and post-control group design. 25 rats were divided into 5 groups. Group I (negative control) were treated by a solution of 0.5% CMC-Na, group II (positive control) were treatedby cholestyramine 0.8 kg / kg, whereas group III, IV and V treated by 96% of ethanolic extract of rind of rambutan with a dose of 125 , 250, and 500 mg / kg respectively. hiperkolesterlemia induction is done by giving a high cholesterol diet and feed for 4 weeks. High-cholesterol diet consists of cooking oil, quail egg yolk, water, PTU and feed high cholesterol consisting of margarine and standard feed. Treatment of mice was conducted for 2 weeks. The results showed that the 96% of ethanolic extract of rind of rambutan fruit with a dose of 125, 250, and 500 mg / kgbw have antihyperkolesterol activity 21.39 ± 6.61%, 31.15 ± 18.15%, and 60.75 ± 8, 26%.Keywords: Antihypercholestrol, Nephelium Lappaceum L., High-cholesterol diet
UJI PRAKLINIK ANTIHIPERURISEMIA SECARA IN VIVO PADA MENCIT PUTIH JANTAN GALUR BALB-C DARI EKSTRAK DAUN SALAM (Syzigium polyanthum Walp) DAN DAUN BELIMBING WULUH (Averrhoa bilimbi L.) Muhtadi, .; Suhendi, Andi; Wahyuningtyas, Nurcahyanti; Sutrisna, EM.
Biomedika Vol 6, No 1 (2014): Biomedika Februari 2014
Publisher : Universitas Muhamadiyah Surakarta

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Abstract

Antihyperuricemia activity in vivo testing has been done of single and combined extracts of Salam (Syzigium polyanthum Walp) and Belimbing Wuluh leaves (Averrhoa bilimbi L.), and also standardized extracts. Salam leaves extraction and Belimbing Wuluh leaves carried by the water solvent. Dried extract of each ingredient had been done antihyperuricemia preclinical in vivo testing against male white mice strain Balb-C induced by potassium oxonate dose of 250 mg / kg b.w. Antihyperuricemia preclinical in vivo testing results showed that the single extracts of Salam and Belimbing Wuluh at a dose of 200 mg / kg b.w. had lower activity levels of uric acid in the blood serum of male white mice, respectively 0.640 and 0.680. While the activity of the combination of extracts of Salam-Belimbing Wuluh is 1.380.Keywords: Antihyperurisemia preclinical in vivo testing; Salam leaves extract, Belimbing Wuluh leaves extract, male white mice Balb-C
PEMISAHAN SENYAWA-SENYAWA YANG BERSIFAT SITOTOKSIK TERHADAP SEL MURIN LEUKEMIA P388 DARI EKSTRAK METANOL KULIT BATANG DIPTEROCARPUS CONFERTUS SLOOT (DIPTEROCARPACEAE) Muhtadi, .; Indrayudha, Peni
Biomedika Vol 5, No 1 (2013): Biomedika Februari 2013
Publisher : Universitas Muhamadiyah Surakarta

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Abstract

Six compounds, KP-1 up to KP-6 were isolated from the methanol extract of the tree bark of Dipterocarpus confertus Sloot (Dipterocarpaceae). The cytotoxic activities of these compounds were evaluated against murine leukemia P388 cells. Result of these examination indicated that KP-2 compound and KP-1 were very active with each IC 50 value of these compounds were 2.25 and 5.1 μg/mL, respectively. While the others were not active against murine leukemia P388 cells.Keywords: Dipterocarpaceae, Dipterocarpus confertus Sloot, isolated compounds, cytotoxic, and leukemia P-388 cell