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Pengaruh Konsentrasi Inokulum Bakteri Zymomonas mobilis dan Lama Fermentasi Pada Produksi Etanol dari Sampah Sayur dan Buah Pasar Wonokromo Surabaya Kusumaningati, Mutiara Arum; Nurhatika, Sri; Muhibuddin, Anton
Jurnal Sains dan Seni ITS Vol 2, No 2 (2013)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM), ITS

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi inokulum bakteri Zymomonas mobilis dan lama fermentasi terhadap produksi etanol yang optimum. Penelitian ini dilakukan pada bulan November 2012 sampai Mei 2013 di Laboratorium Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Teknologi Sepuluh Nopember. Penelitian ini menggunakan variasi perlakuan yaitu konsentrasi inokulum (0, 5, 10 dan 15%) dan lama fermentasi (0, 2, 4, 6, dan 8 hari). Masing-masing perlakuan diulangi sebanyak 2 kali, sehingga diperoleh 40 unit percobaan dan dianalisis menggunakan uji ANOVA. Parameter yang diamati adalah kadar etanol. Hasil yang diperoleh dalam penelitian ini adalah kadar etanol optimum sebesar 9,5% (v/v) dihasilkan pada interaksi konsentrasi inokulum 10% dan lama waktu fermentasi 6 hari
Potensi Alang-alang (Imperata cylindrica (L.) Beauv) dalam Produksi Etanol Menggunakan Bakteri Zymomonas mobilis Kartikasari, Sevy Dwi; Nurhatika, Sri; Muhibuddin, Anton
Jurnal Sains dan Seni ITS Vol 2, No 2 (2013)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM), ITS

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Abstract

Sumber selulosa yang murah dan melimpah dapat diperoleh dari gulma alang-alang (Imperata cylindrica (L.) Beauv). Produksi etanol dari bahan baku selulosa alang-alang umumnya difermentasikan oleh yeast. Pada penelitian ini fermentasi dilakukan menggunakan bakteri Zymomonas mobilis. Penelitian dilakukan pada bulan November 2012 hingga Mei 2013 di laboratorium Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, ITS. Penelitian bertujuan untuk mengetahui potensi alang-alang (I. cylindrica (L) Beauv.) dalam produksi etanol menggunakan bakteri Z. mobilis pada konsentrasi inokulum dan waktu fermentasi yang optimum. Penelitian ini dilakukan dalam tiga langkah utama yaitu pretreatment substrat, hidrolisis enzimatik oleh Penicillium sp., dan fermentasi etanol oleh Z. mobilis. Fermentasi dilakukan menggunakan berbagai perlakuan konsentrasi inokulum (0, 5, 10, dan 15%) dan lama fermentasi (0, 3, 5, 7, dan 9 days) serta dianalisa menggunakan uji Analysis of Varians (ANOVA). Masing-masing perlakuan dilakuan 2 kali pengulangan, sehingga diperoleh 40 unit percobaan dengan parameter yang diamati adalah kadar etanol. Hasil penelitian menunjukkan bahwa alang-alang (I. cylindrica (L.) Beauv.) berpotensi untuk dijadikan sebagai bahan baku produksi etanol dengan konsentrasi etanol tertinggi diperoleh dari interaksi antara 10% konsentrasi inokulum Z. mobilis dan waktu fermentasi 7 hari yaitu sebesar 9,02 % (v/v)
Eksplorasi Mikoriza Vesikular Arbuskular (MVA) Indigenous pada Tanah Aluvial di Kabupaten Pamekasan Madura Cahyani, Ni Kadek Marina Dwi; Nurhatika, Sri; Muhibuddin, Anton
Jurnal Sains dan Seni ITS Vol 3, No 1 (2014)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM), ITS

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Abstract

Mikoriza merupakan simbion mutualistik antara jamur dengan akar tanaman. Kondisi lingkungan yang bervariasi di Indonesia dapat memungkinkan beranekaragamnya komposisi genus mikoriza pada suatu lahan. Penelitian ini bertujuan untuk mengetahui komposisi genus mikoriza vesikular arbuskular indigenous apa sajakah yang diperoleh dari jenis tanah aluvial di Pamekasan Madura.Tanah aluvial diisolasi menggunakan metode tuang saring basah dilanjutkan dengan teknik sentrifugasi sukrosa. Identifikasi dilakukan dengan menggunakan buku panduan Working with Mycorrhizas in Forestry and Agriculture.Hasil penelitian menunjukkan genus mikoriza vesikular arbuskular yang ditemukan tergolong ke dalam tiga genus yaitu Glomus, Gigaspora, dan Acaulospora. Jumlah spora mikoriza tertinggi diperoleh di Pademawu sebesar 11 spora/100 gr dengan 5 tipe genus Glomus, 3 tipe genus Acaulospora dan 3 tipe genus Gigaspora. Sedangkan di Pamekasan sebesar 7 spora/100 gr dengan 6 tipe genus Glomus dan 1 tipe genus Gigaspora. Dan di Tlanakan 9 spora/100 gr dengan 5 tipe genus Glomus dan 4 tipe genus Acaulospora
Eksplorasi Mikoriza Vesikular Arbuskular (MVA) Indigenous pada Tanah Regosol di Pamekasan, Madura Nurhalimah, Siti; Nurhatika, Sri; Muhibuddin, Anton
Jurnal Sains dan Seni ITS Vol 3, No 1 (2014)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM), ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.229 KB)

Abstract

Mikoriza merupakan simbiosis mutualistik antara jamur dengan akar tanaman. Kondisi lingkungan yang bervariasi di Indonesia seperti jenis tanah dapat memungkinkan beranekaragamnya jenis mikoriza pada suatu lahan. Oleh karena itu, penelitian ini bertujuan untuk mengetahui genus Vesikular Arbuskular Mikoriza (MVA) indigenous pada jenis tanah regosol di Pamekasan Madura. Pengambilan sampel dilakukan secara acak. Dari hasil isolasi sampel tanah melalui proses penyaringan basah dan bertingkat dan dilanjutkan teknik sentrifugasi sukrosa, maka di  kecamatan Larangan ditemukan tiga genus spora MVA yaitu genus Glomus, Acaulospora dan Gigaspora dengan jumlah spora sebanyak 7 spora/100 gram tanah yang terdiri dari  3 spora Glomus, 2 spora Acaulospora dan 2 spora Gigaspora. Kecamatan Palengaan ditemukan sebanyak 9 spora/100 gram tanah dengan jumlah Glomus 3 spora, Gigaspora 5 spora dan Acaulospora 1 spora. Serta di kecamatan Pegantenan ditemukan sebanyak 6 spora/100 gram tanah yang terdiri dari  Glomus 2 spora dan Gigaspora 4 spora.
APLIKASI CENDAWAN MIKORIZA ARBUSKULAR (CMA) DAN BOKASHI DALAM MEMINIMALISIR PEMBERIAN PUPUK ANORGANIK PADA PRODUKSI BENIH TANAMAN JAGUNG KETAN (Zea mays ceratina) Ningrum, Dhona Puspita; Muhibuddin, Anton; Sumarni, Titin
Jurnal Produksi Tanaman Vol 1, No 5 (2013)
Publisher : Jurusan Produksi Tanaman Fakultas Pertanian Universitas Brawijaya

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Abstract

Peningkatan produksi jagung di Indonesia kebanyakan dilakukan dengan meningkatkan dosis pupuk anorganik, akan tetapi hasil yang didapat masih rendah, sehingga perlu diupayakan suatu teknologi ramah lingkungan untuk dapat mengefektifkan pemupukan serta memperbaiki kesuburan tanah melalui pemberian bokashi dan penggunaan mikroba potensial seperti cendawan mikoriza arbuskular (CMA). Penelitian bertujuan untuk mengetahui pengaruh aplikasi CMA dan bokashi dalam meminimalisir pupuk anorganik pada produksi benih jagung ketan. Penelitian dilakukan di laboratorium HPT dan kebun percobaan Fakultas Pertanian Malang, dimulai bulan Mei sampai November 2012. Metode penelitian yang digunakan adalah rancangan acak kelompok dengan 10 perlakuan dan 3 ulangan. Perlakuan pupuk anorganik 100%, P1= bokashi+pupuk anorganik 100%, P2= bokashi+pupuk anorganik 75%, P3= bokashi+pupuk anorganik 50%, P4=CMA+pupuk anorganik 100%, P5= CMA+ pupuk anorganik 75%, P6=CMA+pupuk anorganik 50%, P7= CMA+bokashi + pupuk anorganik 100%, P8= CMA+bokashi + pupuk anorganik 75%, P9= CMA+bokashi + pupuk anorganik 50%.Hasil penelitian menunjukkan pemberian bokashi+CMA+pupuk anorganik 75%, bokashi+CMA+pupuk anorganik 100%, bokashi +CMA+ pupuk anorganik 50%, CMA + pupuk anorganik 75% dan CMA + pupuk anorganik 100% mampu meningkatkan hasil biji ton ha-1 masing-masing sebesar 56.66%, 52.22%, 50.51%, 40.27%, dan 38.57% dibandingkan tanaman dengan perlakuan yang hanya menggunakan pupuk anorganik 100%. Penambahan bokashi dan atau CMA dapat meminimalisir pemberian pupuk anorganik pada perlakuan bokashi+ pupuk anorganik 100%, bokashi+ pupuk anorganik 75%, bokashi+pupuk anorganik 50%, CMA+pupuk anorganik 100%, CMA+ pupuk anorganik 75%, CMA+pupuk anorganik 50%, CMA+bokashi+pupuk anorganik 100%, CMA+bokashi+pupuk anorganik 75%, CMA+bokashi+pupuk anorganik 50% masing-masing sebesar 32.76%, 48.55%, 53,75%, 38.57%, 55.20%, 65.36%, 52.22%, 67.50%, dan 75.26%. Kata kunci : Jagung ketan, CMA, bokashi, pupuk anorganik
Correlation between Soil Phospor (P) Content and Soil Yeast Antagonistics Ability Against Fusarium oxysporum Pathogen Causing Wilt Muhibuddin, Anton; Wibowo, Kartika Novitasari; Punnapayak, Hunsa; Goetz, Peter
Inscientech: Journal of Industrial, Science and Technology Vol 1 No 02 (2017): Inscientech: Journal of Industrial, Science and Technology
Publisher : Universitas KH. A. Wahab Hasbullah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32764/inscientech.v1i02.1

Abstract

Besides its role during fermentation process, yeast also has potential as biological agent because of its antagonistics characteristic. We have isolated yeast from six different locations based on Phospourus differences. Isolated yeast were then tested its antagonistics ability of Fusarium oxysporum, the pathogen causing tomato wilt disease. This research aims to find out the diversity of yeast found in the tomato rhizosphere in six different contents of phosphorus locations and to know its antagonistics ability against the pathogenic. F. oxysporum. This research was conducted in the Laboratory of Mycology, Department of Pests and Diseases, Faculty of Agriculture, Brawijaya University, Malang and in the Chemistry Laboratory, Institut Teknologi 10 Nopember Surabaya, started from January up to September 2015. Yeasts have been isolated from tomato’s rhizosphere of 6 different locations around East Java proviences. The result showed that yeasts from organic field (Lower P content) were 6 genera. They are Candida sp. 1, Pichia sp. 1, Hansenula sp., Metschnikowia sp. 1, Cryptococcus sp., and Zygosaccharomyces sp. While the yeasts from inorganic field (higher P) were 3 genera. The most potential yeast in controlling F. oxysporum is Pichia sp. 2. Lower P content showed more divers than higher P content. Yeasts from higher soil P content showed more antagonists to control F. oxysporum. Keywords: Phosphor, correlation, yeast, antagonistic, disease, soil
DECREASE CLUBROOT DISEASE INTENSITY OF Brassica juncea ON Pb CONTAMINATED SOIL USING Paraserianthes falcataria THAT WAS INFECTED BY Mycorrhizal Kurniawan, E; Muhibuddin, Anton; Kusuma, R R
Journal of Environmental Engineering and Sustainable Technology Vol 3, No 1 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jeest.2016.003.01.8

Abstract

Both, biotic and abiotic factor is the most important problem for agriculture in Indonesia. Biotic factor such as plant disease could decrease yield till more than 50%, while abiotic factor such as soil contamination could decrease yield till more than 30%. One of choice to overcome this problem is using bio-phytoremediation method. This method  combine remediation using plant (phytoremediation) and remediation using microbe (bio remediation). This study was aimed to determine the effects of bio-phytotemediation method using Paraserianthes falcataria which infected by mycorrhizal fungi to overcome Phytophthora brassicae, the main clubroot disease on Brassica juncea, in Pb contaminated soil. We also aimed to know the influence of the method to absorb soil Pb using Paraserianthes falcataria. The research was conducted in the Mycology Faculty of Agriculture, University of Brawijaya from March to October 2015. The research used Completely Randomized Design with 6 treatments and 4 replications. Parameter observation in this research were: 1) Intensity of clubroot disease; 2) Growth of B. juncea; 3) Population of mycorrhizal spores in soil,  4) Mycorrhizal infection inside plant cell; and 4) Pb content of soil and plant tissues. The results was showed  that mycorrhizal has significantly effects to decrease clubroot disease intensity. But, mycorrhizal has no significantly effect to the plant growth, including stem length and leaves number. The number of mycorrhizal spores in the soil after 35 days application was increases, and the percentage of infection in the roots of B. juncea and P. falcataria was fluctuated. The application of mycorrhizal can decrease Pb content in the soil and increase Pb content in P. falcataria.
ETHANOL FERMENTATION POTENCY OF WILD YEAST ON BAMBOO RHYZOSPHERE Muhibuddin, Anton
Journal of Environmental Engineering and Sustainable Technology Vol 3, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jeest.2017.003.02.8

Abstract

Bamboo plant rhizosphere known as source of simbiotic useful microorganisms, including yeast. Wild yeast explored should be tested it’s adaptability to new ecology especially nutritional source availability. The research aim to get potential yeast which can work well during fermentation process in apple juice substrate.We were isolated yeast from three different locations: Ketawanggede District, Karangploso District, and Lowokwaru District. All locations was located around Malang city.Result showed that there were found 13 isolates yeast: Protomyces sp, Agaricostilbum sp1, Agaricostilbum sp2, Agaricostilbum sp3, Debaryomyces sp1, Debaryomyces sp2, Debaryomyces sp3,  Trigonopsis sp1, Trigonopsis sp2, Udeniomyces sp1, Udeniomyces sp2, Ascoidea hylocieti, and Komagataella sp. Diversity index indicates medium category to low category and dominance index in all location indicates high category.Fermentation test showed improvements in observation variables at 24 and 72 hours including temperature, cells number, and alcohol percentage. The highest alcohol percentage were 11.6% and 10% that produced by the treatment of Agaricostilbum sp3 and Trigonopsis sp1 respectively.Keyword: Yeast, bamboo plant rhizosphere, apple juice, and fermentation.
Yeast On Epiphyte of Tangerines Citrus Fruit (Citrus nobilis L.) and Their Potential Antagonist to Colletotrichum Gloeosporioides (Penz.) Penz. & Sacc. Pramudita, Oki; Muhibuddin, Anton
Inscientech: Journal of Industrial, Science and Technology Vol 1 No 02 (2017): Inscientech: Journal of Industrial, Science and Technology
Publisher : Universitas KH. A. Wahab Hasbullah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32764/inscientech.v1i02.3

Abstract

One of the most important diseases of citrus plant is anthracnose which caused by Colletotrichum gloeosporioides (Penz.) fungi. Yeast is one of microbes which has a good potential to control the disease. This research focused on exploring yeasts on tangerines and examining their efficacy to control C. gloeosporioides using in vitro and in vivo treatments. Observation was started by isolated C. gloeosporioides and yeast from tangerines citrus fruit, then followed with in vitro and in vitro treatment. Both were counted the percentage of antagonist and pathogen incubation stage and also disease incidence, respectively. Result showed that yeast has been isolated from tangerines fruit were Candida sp. (isolate 1), Candida sp. (isolate 2) and Pichia sp. All yeast showed inhibit C. gloeosporioides growth and suppressed its development.
SOIL DRIVE NUTRIENT AS NEW METHOD FOR TIN MINING REMEDIATION Muhibuddin, Anton
Journal of Environmental Engineering and Sustainable Technology Vol 5, No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Brawijaya

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Abstract

A contaminated soil by tin usually is a big problem in Indonesia. Uniformity is essential for processing soil at a normal quality and to ensure conformity to specify clean up levels. Revegetation efforts were carried out by using rubber and jatropha combination which infected by arbuscular mycorrhizal (VAM) fungi applied using the soil drive nutrient (SDN) method showed good result in the previously research.This research purposes to know the effectivity of soil drive nutrient (SDN) method to promote the growth of soybean plants (Glysine max L.) in tin mining soil. The results showed that the cropping pattern and dose of mycorrhizal gave effect on soybean growth, especially on the trunk diameter. Result also showed that mycorrhizal infection on soybean roots significantly influenced by the SDN method and dose of mycorrhizal that applied. Both of these are expected to have a positive impact on soybean production and resistance to environmental stress and disease attack. Viceversa, height and number of plant leaves untill 28 days were not affected by the SDN method and the dose of mycorrhiza applied.