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Incidence of HPV Infection in Oral Squamous Cell Carcinoma and Its Association with the Presence of p53 & c-myc Mutation : A Case Control Study in Muwardi Hospital Surakarta

Journal of Dentistry Indonesia Vol 17, No 2 (2010): August
Publisher : Faculty of Dentistry, University of Indonesia

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Abstract

Introduction: Annual incidence rates for oral and pharyngeal cancer are estimated at 25 cases per 100,000 in developing countries. Human papilloma virus (HPV) was implicated in pathogenesis of Cancer. The mutations of p53 and c-myc are found 50% in cancer. Objective: Aims of this research were to know the incidence of OSSC patient which realized HPV infection without p53 and c-myc gene mutation. Materials and Methods: Tissue biopsy frozen sections were taken from BOSC (Benign Oral Squamous Cell) and OSCC (Oral Squamous Cell Carcinoma) patients collected from Oral and Dental Departement of dr Muwardi Distric Hospital in Surakarta from January 2007 to January 2008. To amplify L1-HPV gene for fixed the HPV stressor. To amplify p53 and c-myc genes, continued with SSCP (Single Strand Conformational Polymorphisme) analysis and followed with measurement using densitometer, to see mutation existence. The collected data were analyzed with Chi Square. Results: BOSC patient identified 23% with HPV infections and OSCC patient identified 73% with HPV infections. Hundred percent BOSC patient with HPV infection without mutation in p53 gene and c-myc gene, 81% OSCC patient with HPV infection without mutation in p53 gene and 91% OSCC patient with HPV infection without mutation in c-myc gene. Chi  square analysis showed significant difference between BOSC and OSCC patients with HPV infection without mutation in p53 and c-myc gene. Conclusion: HPV is a factor for pathogenesis of OSCC.DOI: 10.14693/jdi.v17i2.44

The Role of Heat Shock Proteins in Pathogenesis of Oral Squamous Cell Carcinoma

Journal of Dentistry Indonesia Vol 16, No 2 (2009): August
Publisher : Faculty of Dentistry, University of Indonesia

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Abstract

Cell in the distress situation, denaturation of proteins may occur, and may also respond by expressing stress proteins. However, such homeostasis effort does not always succeed and even may lead to disease, including cancer. In distress situation also ensue much protein misfolding. Objective: This research were to explain the role of heat shock protein 40 (Hsp40) and Hsp70 in pathogenesis of occurred oral squamous cell carcinoma (OSCC) patient which realized human papilloma virus (HPV) infection. Material and Method: Tissue biopsy frozen section were taken from BOSC and OSCC patients was cut into three part. Parrafin blocks were made from cutting I, which was subsequently stains with HE to ascertain the type of neoplasm. Cutting II was subjected to DNA isolation. The DNA isolation results were subjected to PCR to amplify L1-HPV gene for fixed the HPV stressoor. Protein isolation was treated from Cutting III, folloewd with Blottdot test by using antibody monoclonal anti Hsp40 and Hsp70 and continued with measurement using densitometer to find the concentration of Hsp40 and Hsp70. The collected data were analyzed with F Test (Manova) and discriminant analysis. Result: This experiment showed the differences in concentration of Hsp40 (p<=0,070) and Hsp70 (p<=0,006) between beningn oral squamous cell (BOSC) and OSCC patients which realized HPV infection. Conclusion: This experiment proved that OSCC patients which realized HPV infection indicated an up regulated of Hsp70 concentration, so that there was occurs misfolding of the proteins cell. The misfolding was ensue obstacle of apoptosis and to raise cell proliferation which to storm carcinogenesis. An up regulated of Hsp40 was role as co-chaperone.DOI: 10.14693/jdi.v16i2.91 

Histopathology of Helicobacter pylori in Chronic Dyspepsia Patients

The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 13, NUMBER 3, Desember 2012
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

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Abstract

ABSTRACTBackground: Helicobacter pylori (H. pylori) are the most common infection found in dyspepsia cases. This infection is almost always found in digestive tract inflammation and commonly develops into chronic gastritis. Meanwhile, chronic gastritis is a condition assumed as the early event in pathological abnormalities of the stomach which finally may develop into carcinoma of the gaster. In Indonesia, data describing the incidence of H. pylori infection based on the histopathological appearance, location of specimen collection, inflammatory degree, and age of chronic dyspepsia patients is not yet available. The aim of this study is to determine the incidence of H. pylori based on histopathology appearance in chronic dyspepsia patients in Moewardi Hospital Surakarta.Method: This study is a cross sectional descriptive study by performing endoscopy-biopsy and histopathology examination to chronic dyspepsia patients who came to Gastroenterohepatology Clinic, Department of Internal Medicine, Moewardi Hospital, Surakarta on 1 January 2009 - 31 December 2010.Results: More than 90% subjects were > 40 year old with the distribution of majority patients were 46-55 year old (32.43%). The most commonly found endoscopic appearance in subjects with positive H. pylori was superficial chronic gastritis (81.08%) with mild inflammatory degree (64.86%) and majority located in the antrum 97.3%.Conclusion: The proporsion of H. pylori infection in male and female was almost equal and was mostly found in the age group of 46-55 year old. This infection frequently happens in chronic dyspepsia who has histopathologic appearance of superficial chronic gastritis with mild inflammatory degree in the antrum area. Keywords: Helicobacter pylori, superficial chronic gastritis, age

The Effect of Obesity, Oral Contraceptive and Passive Smoking on the Risk of Cervical Cancer

Journal of Epidemiology and Public Health Vol 4, No 3 (2019)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Abstract

Background: Studies have investigated the effects of obesity on cancer development. However, the relationship between obesity and cervical cancer risk is unclear. This study aimed to determine the effect of obesity, oral contraceptive and passive smoking on the risk of cervical cancer.Subjects and Method: A case-control study was conducted at Dr. Moewardi Hospital, from October to December 2018. A sample of 200 patients was selected by fixed disease sampling, consisting of 100 cervical cancer patients and 100 noncervical cancer patients. The dependent variable was cervical cancer. The independent variables were obesity, oral contraceptive use, smoking exposure, parity, age at first sexual intercourse, and family history. The data were obtained from the medical record. The data were analyzed by multiple logistic regression.Results:The risk of cervical cancer increased with obesity (OR= 6.83; 95%CI= 2.44 to 19.17; p<0.001), cigarette smoke exposure (OR= 12.57; 95% CI= 4.59 to 34.41; p<0.001),oral contra­ceptive use (OR= 3.43; 95%CI= 1.27 to 9.25; p= 0.015), parity (OR= 3.94; 95%CI= 1.47 to 10.59; p= 0.006), and family history (OR= 5.63; 95%CI= 1.94 to 16.34; p= 0.001).The risk of cervical cancer decreased with delayed menarche (OR= 0.24; 95%CI= 0.09 to 0.68; p= 0.007) and delayed age at first sexual intercourse (OR= 0.21; 95%CI= 0.86 to 0.53; p= 0.001).Conclusion: The risk of cervical cancer increases with obesity, oral contraceptive use, smoking exposure, parity, and family history. The risk of cervical cancer decreases with delayed menarche and delayed age at first sexual intercourse.Keywords: obesity, oral contraception, smoking exposure, cervical cancerCorrespondence: Roiela Arfailasufandi. Masters Program in Public Health, Universitas Sebelas Maret. Jl. Ir. Sutami No. 36 A, Surakarta 57126, Central Java, Indonesia. Email: andi_suf@ymail.com. Journal of Epidemiology and Public Health (2019), 4(3): 189-197https://doi.org/10.26911/jepublichealth.2019.04.03.06

Determinants of Recurrence and Death in HIV-Malaria Co-Infection Patients in Jayapura, Papua, Indonesia

Journal of Epidemiology and Public Health Vol 4, No 3 (2019)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Abstract

Background: Human Immunodeficiency Virus (HIV) is one of the important public health problems in the world which causes death of more than 35 million people annually. HIV infection is often associated with several comorbidities caused by the presence of endemic infectious diseases in certain regions, one of them is malaria. Analyzing the survival of HIV / AIDS patients malaria coinfection is based on risk factors for recurrence and death due to malaria. This study aimed to examine the determinants of recurrence and death in HIV-malaria co-infection patients in Jayapura, Papua, Indonesia.Subjects and Method: This was a retrospective cohort study conducted in the VCT room at Dok II Regional General Hospital, Jayapura, Papua Province, Indonesia, from May to June 2018. A sample of 183 people living with HIV/AIDS (PLWH) was selected by fixed disease sampling. The dependent variable was recurrence. The independent variables were drug taking adherence, nutritional status, economic status, use of mosquito nets, spraying residues in the house, healthy hygiene behavior, co-infection with malaria. The data were obtained from medical record. The other data were collected by questionnaire. The data were analyzed using path analysis.Results: The risk of recurrence in PLWH co-infected with malaria decreased with ARV treatment adherence (b= -2.17; 95% CI= -3.24 to -1.09; p<0.001) and compliance with Cotrimoxazole treatment (> 95 %) (b= -1.88; 95% CI= -2.58 to -1.19; p<0.001). The risk of recurrence in PLWHA co-infected with malaria increased with low income 95%) (b= -2.08; 95% CI= -3.02 to -1.14; p<0.001) and healthy behavior (mean> 5) (b= -1.43; 95% CI= -2.15 to -0.70; p<0.001). The risk of death in PLWHA co-infected with malaria increased with low income (

Health Belief Model on the Determinants of Human Papilloma Virus Vaccination in Women of Reproductive Age in Surakarta, Central Java

Journal of Health Promotion and Behavior Vol 3, No 1 (2018)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Abstract

Background: Cervical cancer is a type of cancer that develops in a woman's cervix. Cervical cancer is caused by a human papillomavirus (HPV). Cervical cancer ranked fourth against most women's cancer. The incidence of cervical cancer was estimated at 528,000 (7.9%) cases worldwide causing 266,000 deaths in 2012. The HPV vaccine can protect women from HPV infection, thus lowering the risk of cervical cancer. This study aimed to analyze the determinants of HPV vaccination in women of reproductive age in Permata Harapan Oncology Clinic, Surakarta, Central Java, using Health Belief Model.Subjects and Method: This was an analytic observational study with a case-control design. This study was conducted at Permata Harapan Oncology Clinic, Surakarta, from January to February 2018. A total study of 200 study subjects was selected by fixed disease sampling. The dependent variable was HPV vaccination. The independent variables were knowledge, perceived seriousness, perceived susceptibility, perceived barrier, perceived benefit, family income, and family support. The data were collected by questionnaire and analyzed by a multiple logistic regression.Results: HPV vaccination increased with better knowledge (OR=7.97; 95% CI= 1.50 to 42.38; p= 0.015), perceived seriousness (OR=22.81; 95% CI= 6.06 to 85.86; p<0.001), perceived susceptibility (OR=4.03; 95% CI= 1.25 to 13.09; p=0.020), , perceived benefit (OR­=6.57; 95% CI= 1.88 to 22.98; p=0.003), family income (OR=5.32; 95% CI= 1.57 to 18.07; p=0.007), and family support (OR=6.86; 95% CI= 1.55 to 30.36; p= 0.011). HPV vaccination decreased with perceived barrier (OR=0.14; 95% CI= 0.04 to 0.51; p=0.003).Conclusion: HPV vaccination increases with better knowledge perceived seriousness, perceived susceptibility, perceived benefit, family income, and family support, but decreases with perceived barrier.Keywords: HPV vaccination, knowledge, perceived seriousness, perceived susceptibility, perceived barrier, perceived benefit, family income, family supportCorrespondence: Yulia Fitriani. Masters Program in Public Health, Sebelas Maret University, Jl. Ir. Sutami 36 A, Surakarta 57126, Central Java. Email: yuliafitriani38@gmail.com. Mobile: 085655708946.Journal of Health Promotion and Behavior (2018), 3(1): 16-26https://doi.org/10.26911/thejhpb.2018.03.01.02 

Effect of Breast Care and Oxytocin Massage on Breast Milk Production: A study in Sukoharjo Provincial Hospital

Journal of Maternal and Child Health Vol 1, No 2 (2016)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Abstract

Background: The prevalence of exclusive breast feeding was 39.05% in Sukoharjo in 2015, which is far bellow the national target of 80%. A study has shown that massage oxytocin increases oxytocin (OT) hormone release, and eventually decreases adrenocorticotropin hormone (ACTH), nitric oxide (NO), and beta-endorphin (BE). This OT hormone release will increase milk ejection, which facilitate milk production. This study aimed to investigate the effect of breast care and oxytocin massages on breast milk production in post–partum mothers.Subjects and method: This was a Randomized Controlled Trial (RCT), conducted at Sukoharjo Hospital, Central Java 19 October to November 18, 2016. A total of 90 post – partum mothers were selected at random and then allocated into breast care group and oxytocin massage group. The dependent variable was breast milk production. The independent variable was breast care and oxytocin massage. Changes in breast milk production before and after intervention between the two groups were tested by Mann-Whitney test.Results: The increase in breast milk production in breast care and oxytocin massage group (mean = 17.37, SD = 9.70) was larger than that of the control group (mean = 1.58, SD = 1.69), and it was statistically significant (p <0.001).Conclusion: Breast care and oxytocin massage can significantly increase breast milk production. Post-partum mothers are recommended to practice breast care and oxytocin massage, in order to increase breast milk production.Keywords: breast care, oxytocin massage, breast milk production.Correspondende: Tutik Rahayuningsih. School of Health Polytechnics, Poltekkes Bhakti Mulia, Surakarta. Email: tutikrahayu_abm@yahoo.co.idJournal of Maternal and Child Health (2016), 1(2): 101-109https://doi.org/10.26911/thejmch.2016.01.02.05

Histopathology of Helicobacter pylori in Chronic Dyspepsia Patients

The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 13, NUMBER 3, Desember 2012
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

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Abstract

ABSTRACTBackground: Helicobacter pylori (H. pylori) are the most common infection found in dyspepsia cases. This infection is almost always found in digestive tract inflammation and commonly develops into chronic gastritis. Meanwhile, chronic gastritis is a condition assumed as the early event in pathological abnormalities of the stomach which finally may develop into carcinoma of the gaster. In Indonesia, data describing the incidence of H. pylori infection based on the histopathological appearance, location of specimen collection, inflammatory degree, and age of chronic dyspepsia patients is not yet available. The aim of this study is to determine the incidence of H. pylori based on histopathology appearance in chronic dyspepsia patients in Moewardi Hospital Surakarta.Method: This study is a cross sectional descriptive study by performing endoscopy-biopsy and histopathology examination to chronic dyspepsia patients who came to Gastroenterohepatology Clinic, Department of Internal Medicine, Moewardi Hospital, Surakarta on 1 January 2009 - 31 December 2010.Results: More than 90% subjects were > 40 year old with the distribution of majority patients were 46-55 year old (32.43%). The most commonly found endoscopic appearance in subjects with positive H. pylori was superficial chronic gastritis (81.08%) with mild inflammatory degree (64.86%) and majority located in the antrum 97.3%.Conclusion: The proporsion of H. pylori infection in male and female was almost equal and was mostly found in the age group of 46-55 year old. This infection frequently happens in chronic dyspepsia who has histopathologic appearance of superficial chronic gastritis with mild inflammatory degree in the antrum area. Keywords: Helicobacter pylori, superficial chronic gastritis, age

Predictors of Hypertension in Post-Menopausal Women in Surakarta, Central Java

Journal of Epidemiology and Public Health Vol 3, No 3 (2018)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Abstract

Background: Hypertension caused 45% mortality of heart disease and 51% mortality of stroke in 2014. Young women have a lower risk of hypertension than men. The risk increases after menopause due to lower estrogen production. This study aimed to estimate the biopsychosocial factors affecting hypertension in post-menopausal women in Surakarta, Central Java.Subjects and Method: This was an analytic observational study with a cross-sectional design. The study was conducted in Surakarta, Central Java, from January to February 2018. A sample of 200 study subjects was selected by fixed disease sampling. The dependent variable was hypertension. The independent variables were obesity, healthy diet, history of contraceptive use, family support, and family history of hypertension. Data of blood pressure was measured by sphygmomanometer. Other variables were measured by questionnaire. The data were analyzed by a multiple logistic regression.Results: The risk of hypertensionin post-menopuse women increased with obesity (OR= 13.00; 95% CI=2.99 to 56.51; p=0.001), family history of hypertension (OR=9.99; 95% CI=2.54 to 39.19; p=0.001), and history of hormonal contraceptive use (OR=10.11; 95% CI=2.60 to 39.25; p=0.001). The risk of hypertension in post-menopuse women decreased with healthy diet (OR= 0.16; 95% CI= 0.04 to 0.55; p= 0.004) and strong family support (OR= 0.09; 95% CI=0.19 to 0.39; p=0.001).Conclusion: The risk of hypertension post-menopause women is affected by obesity, family history of hypertension, history of hormonal contraceptive use, healthy diet, and strong family support. Journal of Epidemiology and Public Health (2018), 3(3): 312-322https://doi.org/10.26911/jepublichealth.2018.03.03.02

Risk Factors of Breast Cancer in Women at Dr. Moewardi Hospital, Surakarta, Central Java

Journal of Epidemiology and Public Health Vol 3, No 2 (2018)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Abstract

Background: Breast cancer is a leading type of cancer affecting women around the world. Its incidence is increasing in developed and developing countries. Breast cancer is the leading cause of death among women in Indonesia with 19,731 cases of death in 2014. In addition to mortality, breast cancer causes psychosocial effect in the affected and the family. This study aimed to determine the risk factors of breast cancer in women at Dr. Moewardi Hospital, Surakarta, Central Java.Subjects and Method: This was an analytical observational study with case control design. The study was conducted at Dr. Moewardi Hospital, Surakarta, from October to December, 2017. A total sample of 200 study subjects consisting of 100 women with breast cancer and 100 women without breast cancer were selected for this study by fixed disease sampling. The dependent variable was breast cancer. The independent variables were age at menarche, age at first pregnancy, parity, duration of breastfeeding, use of fertility hormone, duration of hormonal contraceptive use, and age at menopause. The data was collected  by questionnaire and analyzed by multiple logistic regression.Results: The risk of breast cancer increased with earlier age at menarche <12 years old (OR= 2.71; 95% CI= 1.06 to 6.96; p= 0.037), delayed age at first pregnancy ≥30 years old (OR=3.01; 95% CI= 1.16 to 7.78; p= 0.023), delayed age at menopause ≥55 years old (OR=1.07; 95% CI=0.82 to 6.30; p= 0.001), longer hormonal contraceptive use ≥10 years (OR=3.25; 95% CI= 1.20 to 9.63 p= 0.003).Conclusion: The risk of breast cancer increases with earlier age at menarche, delayed age at first pregnancy, delayed age at menopause, longer hormonal contraceptive use.Keyword: Breast Cancer, Risk FactorCorrespondence: Yana Listyawardhani. Masters Program in Public Health, Universitas Sebelas Maret, Jl. Ir. Sutami 36 A, Surakarta 57126, Central Java. Email:yanalistya@gmail.comJournal of Epidemiology and Public Health (2018), 3(2): 118-127https://doi.org/10.26911/jepublichealth.2018.03.02.02