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Corelation Between Matrix Metalloproteinase-9 (MMP-9) With Complications of Acute Heart Failure In Myocardial Infarction With ST-Elevation (STEMI) And Acute Coronary Syndromes Without STElevation (NSTEACS) Setianto, Budi Yuli; Mubarika, Sofia; Irawan, Bambang
Jurnal Kardiologi Indonesia Vol 32, No 4 (2011)
Publisher : Indonesian Heart Association

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Abstract

Background. Acute coronary syndrome (ACS) often leads tocomplications of acute heart failure. These complications will increasethe morbidity and mortality of patients with ACS.Objective. To determine differences in levels of MMP-9 betweenSTEMI and NSTEACS and the correlation between MMP-9 with acuteheart failure between the two groups.Methods. Examination of the samples performed in 79 patientswith ACS (38 STEMI and 41 NSTEACS) prior to the action ofintravenous thrombolytic or coronary intervention. Differences inlevels of MMP-9 in the ACS are experiencing acute heart failure andwithout heart failure, and differences in levels of MMP-9 in the STEMI andNSTEACS groups were tested with Chi-square, Fisher’s exact test or theIndependent t-test.Results. STEMI groups had significantly higher levels of MMP-9 thanNSTEACS group 1629.12 ± 719.60 compared to 1033.42 ± 777.12(p = 0.001). However, STEMI groups who have acuteheart failureare higher but not significant compared with NSTEACS group 14(36.84) and 11 (26.82) (p = 0.339). There are differences in levelsof MMP-9 in ACS with acute heart failure than those who did not:1698 ± 867.95 ng/mL and 1144.61 ± 713.60 ng/mL (p = 0.004).Conclusion. MMP-9 levels are significantly higher in STEMI groupscompared with NSTEACS groups, and MMP-9 associated with the incidenceofacute heart failure in ACS. STEMI groups have tended to have acute heartfailure are higher than NSTEACS groups.
Corelation Between Matrix Metalloproteinase-9 (MMP-9) With Complications of Acute Heart Failure In Myocardial Infarction With ST-Elevation (STEMI) And Acute Coronary Syndromes Without STElevation (NSTEACS) Setianto, Budi Yuli; Mubarika, Sofia; Astuti, Indwiani; Irawan, Bambang
Jurnal Kardiologi Indonesia Vol 34, No 4 (2011): Vol 32, No 4 (2011): Oktober-Desember 2011
Publisher : Indonesian Heart Association

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Abstract

Background. Acute coronary syndrome (ACS) often leads to complications of acute heart failure. These complications will increase the morbidity and mortality of patients with ACS.Objective. To determine differences in levels of MMP-9 between STEMI and NSTEACS and the correlation between MMP-9 with acute heart failure between the two groups.Methods. Examination of the samples performed in 79 patients with ACS (38 STEMI and 41 NSTEACS) prior to the action of intravenous thrombolytic or coronary intervention. Differences in levels of MMP-9 in the ACS are experiencing acute heart failure and without heart failure, and differences in levels of MMP-9 in the STEMI and NSTEACS groups were tested with Chi-square, Fisher’s exact test or the Independent t-test.Results. STEMI groups had significantly higher levels of MMP-9 than NSTEACS group 1629.12 ± 719.60 compared to 1033.42 ± 777.12 (p = 0.001). However, STEMI groups who have acuteheart failure are higher but not significant compared with NSTEACS group 14(36.84) and 11 (26.82) (p = 0.339). There are differences in levels of MMP-9 in ACS with acute heart failure than those who did not: 1698 ± 867.95 ng/mL and 1144.61 ± 713.60 ng/mL (p = 0.004).Conclusion. MMP-9 levels are significantly higher in STEMI groups compared with NSTEACS groups, and MMP-9 associated with the incidence of acute heart failure in ACS. STEMI groups have tended to have acute heart failure are higher than NSTEACS groups.
Corelation Between Matrix Metalloproteinase-9 (mmp-9) And Troponin-I (cTn-I) in ST- Elevation Myocardial Infarction (STEMI) and Non St-Elevation Acute Coronary Syndrome (NSTEACS) Setianto, Budi Yuli; Astuti, Indwiani; Irawan, Bambang; Mubarika, Sofia
Jurnal Kardiologi Indonesia Vol. 32 No.1 Jan-Mar 2011
Publisher : Indonesian Heart Association

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Abstract

Background: Acute coronary syndrome (ACS) is due to plaque rupture or erosion. Plaque rupture or erosion occurs because of the extra-cellular matrix destruction by an MMP (matrix metalloproteinase). Troponin I(cTn-I) is a biomarker that will increase in ACS with myocardial necrosis.Objective: To determine levels of MMP-9 difference between STEMI and NSTEACS and the relationship between levels of MMP-9 and cTn-I levels between the two groups.Methods: The sample examination performed in 80 patients with ACS (39 STEMI and 41 with NSTE-ACS) prior to the act of intravenous thrombolysis or coronary intervention.Analysis of MMP-9 levels relationship and cTn-I using Spearman test, and analysis of the cut-off relationship MMP-9 in the STEMI and NSTEACS groups were tested by Chi square.Results: Group STEMI had higher levels of MMP-9 and significantly higher compared with NSTE-ACS group (p = 0.002). Spearman correlation test showed a significant and positive correlation between MMP-9 and troponin-Ibetween the two groups (p = 0.003 and r = 0.33).Conclusion: Increased levels of MMP-9 were significantly higher in STEMI compared with NSTE-ACS and its association with elevated levels of troponin-I, provide information about the role of MMP-9 against the severity of heart muscle damage that occurred.Keywords: MMP-9, Troponin-I, STEMI, NSTEACSPendahuluan: Sindrom koroner akut (SKA) terjadi karena adanya ruptur atau erosi plak. Ruptur atau erosi plak terjadi karena adanya destruksi matriks ekstra selular oleh suatu MMP (matriks metaloproteinase). Troponin I (cTn-I) merupakan biomarker yang akan meningkat pada SKA dengan nekrosis miokard.Tujuan: Untuk mengetahui perbedaan kadar MMP-9 antara STEMI dan NSTEACS serta hubungan antara kadar MMP-9 dan kadar cTn-I diantara kedua kelompok.Metode: Pemeriksaan sampel dilakukan pada 80 pasien SKA (39 STEMI dan 41 NSTEACS) sebelum dilakukan tindakantrombolisis intravena atau intervensi koroner. Analisis hubungan kadar MMP-9 dan cTn-I menggunakan uji Spearman, dan analisis hubungan cut-off MMP-9 pada kelompok STEMI dan NSTEACS diuji dengan chi square.Hasil: Kelompok STEMI memiliki kadar MMP-9 yang lebih tinggi dan bermakna dibandingkan kelompok NSTEACS (p=0.002). Uji korelasi Spearman menunjukkan hubungan yang signifikan dan korelasi positif antara MMP-9 dan troponin-I diantara kedua kelompok (p=0.003 dan r=0.33).Kesimpulan: Peningkatan kadar MMP-9 yang lebih tinggi secara bermakna pada STEMI dibandingkan dengan NSTEACS dan hubungannya dengan peningkatan kadar troponin-I, memberikan informasi tentang peran MMP-9 terhadap beratnya kerusakan otot jantung yang terjadi.Kata kunci: MMP-9, Troponin-I, STEMI, NSTEACS
AKTIVITAS KEMOPREVENSI EKSTRAK TEMU KUNCI (Boesenbergia pandurata) PADA KARSINOGENESIS KULIT MENCIT BALB/C TERINDUKSI RADIASI ULTRA VIOLET Listyawati, Shanti; Sismindari, Sismindari; Mubarika, Sofia; B. Murti, Yosi
Prosiding Seminar Biologi Vol 9, No 1 (2012): Seminar Nasional IX Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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ABSTRAK   Temu kunci (B.pandurata) mengandung senyawa-senyawa yang berpotensi antikanker. Penelitian ini bertujuan untuk mengkaji efek kemoprevensi ekstrak etanolik rimpang temu kunci pada karsinogenesis kulit terinduksi ultra violet. Ekstraksi serbuk rimpang B. pandurata dilakukan dengan metode maserasi  menggunakan etanol. Hewan uji yang digunakan adalah mencit Balb/C betina umur 28 hari yang dicukur rambut punggungnya, dikelompokkan menjadi 5 kelompok perlakuan, yaitu kelompok kontrol dan empat  kelompok   perlakuan ekstrak etanolik temu kunci yang diberikan secara oral. Induksi karsinogenesis dengan paparan UV dosis 0,167 J/cm2/hari, sebanyak 60 paparan (5 kali paparan/minggu). Efek penghambatan karsinogenesis kulit  dipelajari pada tingkat insidensi dan multiplisitas kanker. Hasil uji menunjukkan bahwa ekstrak etanolik temu kunci mampu menurunkan angka insidensi dan multiplisitas kanker kulit pada mencit Balb/C terinduksi UV. Ekstrak tersebut berpotensi   dikembangkan sebagai agen kemoprevensi kanker.   Kata Kunci: Boesenbergia pandurata, kemoprevensi, karsinogenesis , ultra violet,
Efek sitotoksik in vitro dari ekstrak daun mimba (Azadirachta indica A. Juss) terhadap beberapa jenis lini sel kanker manusia Wahyuningsih, Mae S.H.; Mubarika, Sofia
Jurnal Kedokteran Yarsi Vol 10, No 3 (2002): Jurnal Kedokteran Yarsi
Publisher : Jurnal Kedokteran Yarsi

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ABSTRACTCancer is still a fearful disease in the world and an ideal medicine has not been found yet. The leaves of mimba (Azadirachta indica A. Jus.) have been reported to be used as an anticancer agent, however no scientific reports have been published so far. This study is aimed to test the cytotoxic effect of the leaves on 7 types of human cancer cell lines invitro, and eventually to isolate and identify the compounds responsible for cytotoxic activity. The leaves were extracted with chloroform followed by methanol to give chloroform(AiCh) and methanol (AiMe) extracts respectively. The two extracts were tested for their cytotoxic activity on 7 types of human cancer cell lines (A498, kidney; EVSA-T, breast;H226, lung; IGROV, ovarian; M19, melanoma; MCF7, breast; WiDR, colon) applying the sulforhodamine B (SRB) method. Doxorubicine and cisplatin were used as positive controls.Based on the IC, of AiCh and AiMe, AiCh is considered to be more potent than AiMe as its IC, is similar to that of cisplatine on EVSA-T, H226, M19, IGROV; and its IC, is even lower than that of cisplatin on A498, MFC7 and WiDR.KEYWORDS cytotoxicity; human cancer cell lines; azadirachta indica; sulforhodamine B (SRB)- assay
ISOLASI DAN AMPLIFIKASI GEN PENYANDI DOMAIN C-TERMINUS Latent Membrane Protein (Imp-I ) Epstein-barr Virus(EBV) DARI PENDERITA KARSINOMA NASOFARING (KNF) Haryanto, Aris; Mubarika, Sofia
Jurnal Sain Veteriner Vol 18, No 2 (2000)
Publisher : Fakultas Kedokteran Hewan

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AKTIVITAS KEMOPREVENSI EKSTRAK TEMU KUNCI (Boesenbergia pandurata) PADA KARSINOGENESIS KULIT MENCIT BALB/C TERINDUKSI RADIASI ULTRA VIOLET Listyawati, Shanti; Sismindari, Sismindari; Mubarika, Sofia; B. Murti, Yosi
Prosiding Seminar Biologi Vol 9, No 1 (2012): Seminar Nasional IX Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (342.135 KB)

Abstract

ABSTRAK   Temu kunci (B.pandurata) mengandung senyawa-senyawa yang berpotensi antikanker. Penelitian ini bertujuan untuk mengkaji efek kemoprevensi ekstrak etanolik rimpang temu kunci pada karsinogenesis kulit terinduksi ultra violet. Ekstraksi serbuk rimpang B. pandurata dilakukan dengan metode maserasi  menggunakan etanol. Hewan uji yang digunakan adalah mencit Balb/C betina umur 28 hari yang dicukur rambut punggungnya, dikelompokkan menjadi 5 kelompok perlakuan, yaitu kelompok kontrol dan empat  kelompok   perlakuan ekstrak etanolik temu kunci yang diberikan secara oral. Induksi karsinogenesis dengan paparan UV dosis 0,167 J/cm2/hari, sebanyak 60 paparan (5 kali paparan/minggu). Efek penghambatan karsinogenesis kulit  dipelajari pada tingkat insidensi dan multiplisitas kanker. Hasil uji menunjukkan bahwa ekstrak etanolik temu kunci mampu menurunkan angka insidensi dan multiplisitas kanker kulit pada mencit Balb/C terinduksi UV. Ekstrak tersebut berpotensi   dikembangkan sebagai agen kemoprevensi kanker.   Kata Kunci: Boesenbergia pandurata, kemoprevensi, karsinogenesis , ultra violet,
Learning Vector Quantization Pada Pengenalan Pola Tandatangan Mubarika, Sofia; Munir, M.; Firdausi, Ketut Sofjan; Widarto, Widarto
JURNAL SAINS DAN MATEMATIKA Volume 14 issue 4 Year 2006
Publisher : JURNAL SAINS DAN MATEMATIKA

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ABSTRACT---In this paper, we analyze and compute distribution of thermal neutron flux in core of Kartini’s Reactor using “Swadaya” detector, both for axial (function of z) and radial (function r) direction. First, “Swadaya” detector is calibrated by activation plate method, which the output from detector is current. The result of calibration is thermal neutron flux (ф) dependent on current (I) that can be written as ф = 4,57x1020I - 2,74x1011. Distribution for neutron thermal flux for axial direction and radial direction respectively Ф = 5,10 x 1012 + 5,99 x 1013 and ф = 1.08 x 1012 + 1,00 x 103 which in reactor core ф has largest value in center position for radial direction.   Keywords: flux neutron distribution, nucleus reactor, Swadaya detector
PREPARATION OF LYMPHOCYTE CULTURE CELL FROM PERIPHERIAL BLOOD OF NASOPHARYNGEAL CARCINOMA PATIENTS Haryanto, Aris; Mubarika, Sofia; Wijayanti, Nastiti
Jurnal Sain Veteriner Vol 18, No 1&2 (2000)
Publisher : Fakultas Kedokteran Hewan

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Lymphocyte is leukocyte component that difficult to culture in vitro. Several viruses need lymphocytes as host cell in order to proliferate and growth in this media such as Epstein-Barr virus (EBV). This virus was associated with malignant disease like nasopharyngeal carcinoma (NPC). The objectives of this research are to develop and to prepare lymphocyte cell culture as material source of DNA for molecular analysis of virus. Peripheral blood was collected from NPC patients which is histopatologically and serologically positive of EBV. Lymphocytes were separated from the other blood components using ficcol-histopaque. Lymphocytes were diluted using RPMt medium then they were cultivated into 96 microwell plate with concentration of 106 cell/ml. The medium consist of 10% FBS, RPMI, Penstrep and FK 506. Culture of lymphocytes were incubated in 5% CO2 at 37°C. The lymphocyte cultures developed and grew confluendy during the first week. Only B cells whith EBV would be well establish. After 50 cell generations, lymphocytes were transformed and immortalized to be lymphoblastoid cell fine (LCL).
Impact of Curcuma mangga Val. Rhizome Essential Oil to p53, Bcl-2, H-Ras and Caspase-9 expression of Myeloma Cell Line Astuti, Endang; Sunarminingsih, Retno; Jenie, Umar Anggara; Mubarika, Sofia; S, Sismindari
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

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Cancer is a disease, a public health problem, which is found in the world as well as in Indonesia. Ingeneral, some of cancer theraphies are ineffective, characterized by the resistance performance of cancer cell line,the exposed normal cell and by the side effects. Nowadays, studies to fi nd the specifi c and safely anti-cancerdrugs were increased by the time. Several studies revealed that Curcuma mangga Val. Rhizome contains somesecondary metabolites, essential or non-essential oil, which has cytotoxic activities to the cancer cells. Basedon these anti-cancer potentials, this study has several aims to recognize anti-cancer selectivity and molecularmechanism by inducting apoptosis and inhibiting myeloma cell proliferation. To C. mangga Val. essential oil,immunocyto chemical test was performed to determine the expression of p53, caspase-9, Bcl-2, H-Ras proteinwhile TUNEL test was performed to determine the number of apoptosis cells.The results of this study shown that anti-cancer molecular mechanism of C. mangga Val. essential oil tomyeloma cell line was performed by increasing apoptosis; by increasing the expression of pro-apoptosis p53,caspase-9 protein and reducing protein which is increasing proliferation Bcl-2 and H-Ras.