Johanes C. Mose
Department of Obstetric and Gynecology, Faculty of Medicine, Padjajaran University, Bandung

Published : 10 Documents
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Correlation between insulin-like growth factor - 1, iron deficiency anemia, and fetal biometry in the third trimester of pregnancy

Indonesian Journal of Obstetrics and Gynecology Vol. 33. no. 3 July 2009
Publisher : Indonesian Journal of Obstetrics and Gynecology

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Abstract

Objective: To analyze correlation between maternal serum IGF-1, iron deficiency anemia and, fetal biometry in 3rd trimester of pregnancy.Setting: Obstetrics and Gynecology clinic Dr. Wahidin Sudirohusodo Hospital and Siti Fatimah Mother and Child Hospital in Makassar.Design/data identification: Cross sectional study.Material and methods: Level of Hb, ferritin, and IGF-1 serum, was taken for laboratory analysis from 70 pregnant women (34 with iron deficiency anemia and 36 normal). Fetal biometry were performed using ultrasonography.Results: There was a significant correlation between maternal serum IGF-1 and fetal AC and FL measurement (p = 0,0010 and p = 0,013, respectively). There is a positive correlation between maternal serum IGF-1 and fetal AC (r = 0,350 and p = 0,001) and FL measurement (r = 0,320 and p = 0,003). Iron deficiency anemia maternal in third trimester of pregnancy had a positive correlation with fetal AC measurement (p = 0,0450).Conclusion: In 3rd trimester of pregnancy: maternal serum IGF-1 was not different between iron-deficiency anemia and normal but there was a positive correlation between maternal serum IGF-1 level with fetal AC and FL measurement and between iron deficiency anemia mothers and with fetal AC measurement.Keywords: IGF-1, iron deficiency anemia, fetal biometry

Aspects of Ethics in Obstetrics Ultrasound

Indonesian Journal of Obstetrics and Gynecology Vol. 32. No. 2. April 2008
Publisher : Indonesian Journal of Obstetrics and Gynecology

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AbstrackObjective: To elaborate some ethical aspects corespond to Obstetrical Ultrasound examination.Material and methods: An observational study based on routine ultrasound examinations and results from literature review on ethical aspects that cover moral and legal problems due to the tremendous application of ultrasounds in obstetrics lately.Results: Ethics is defined as the disciplined study of morality. Four principles in medical ethics i.e. beneficence, non-maleficence, autonomy,and justice, were used as the starting points in exploring the various ethical aspects in conducting obstetrical ultrasound examination. Ethical issues may arise from various aspects such as type of instruments and technique of examination, operator, indication, way of conduct, and some special cases such as sex determination, prenatal informed consent for sonogram (PICS), screening for congenital anomalies, and boutique fetal imaging.Conclusion: Ethical problem in obstetrical ultrasound examination correlates tightly with various aspects such as, type of instruments and technique of examination, competency of the operator, indication, etiquette, and some specific cases among others, sex determination, PICS, screening for congenital anomalies, and boutique fetal imaging.Keywords: ethics, obstetrical ultrasound

Correlations of Chronic Periodontitis with Preeclampsia and Fetal Birth Weight

Indonesian Journal of Obstetrics and Gynecology Vol. 35. No 3. July 2011
Publisher : Indonesian Journal of Obstetrics and Gynecology

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Abstract

AbstractObjective: Preeclampsia is the main cause of maternal and perinatal mortality and morbidity one of which low birth weight. The cause of preeclampsia is multifactorial, and one of them is infection. High periodontal incidence is found in preeclampsia patients. This study is intended to find correlations between chronic periodontitis with preeclampsia and fetal birth weight.Method: The study was held in RS Dr. Hasan Sadikin Bandung, RSUD Astana Anyar and RSUD Ujung Berung in December 2010 - February 2011 using case-control study design in two groups,with 26 subjects in each group. The subjects were recorded on age, parity, gestational age, diagnosis, fetal birth weight, and periodontal status. Statistical analysis were performed using chi square test and Rank Spearman’s correlation test.Result: The characteristics of study subjects in both groups show no significant difference (p > 0.05). In the preeclampsia group, periodontitis incidence is higher which is 19 (73.08%) compared to control group which is 12 (46.15%). Based on chi square method, there is a significant correlation between periodontitis and preeclampsia with odds ratio value of 3.17 (p = 0.048; p < 0.05; 95%CI (1.19 - 8.41). Based on Rank-Spearman correlation test, there is a moderate negative correlation between periodontitis severity and with fetal birth weight with rs value of = - 0.423 and a p value of 0.002.Conclusion: Chronic periodontitis incidence is higher in preeclampsia group compared to control group. There’s a moderate negative correlation on periodontitis severity with fetal birth weight. The more severe the periodontitis, the lower the fetal birth weight.[Indones J Obstet Gynecol 2011; 35-3: 105-9]Keywords: periodontitis, preeclampsia, fetal birth weight

Correlation of Total Antioxidant Capacity Measured by Ferric Reducing Ability of Plasma (FRAP) Assay with the Severity of Preeclampsia

Indonesian Journal of Obstetrics and Gynecology Vol. 35. No 4. October 2011
Publisher : Indonesian Journal of Obstetrics and Gynecology

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Abstract

AbstractObjective: To analyze correlation between total antioxidant capacity measured by Ferric Reducing Ability of Plasma (FRAP) assay with severity of preeclampsia.Method: The cross sectional method was used in this study to compare TAC of four different groups of study, consists of normal pregnancy, mild preeclampsia, severe preeclampsia and eclampsia. The study consisted of 15 women in each group. All of the subjects met the inclusion criteria and were admitted to Dr. Hasan Sadikin Hospital and it’s district hospital. The study was conducted from August until September 2011. 3 ml blood samples were taken and were measured by FRAP assay in the laboratory PRODIA Jakarta.Result: There was no significant difference (p<0.05) on subject’s characteristics based on number of parity and gestational age among the groups of study. The comparison of TAC measured by FRAP assay based on ANOVA was significant difference (p<0.01). The highest mean FRAP assay result appeared in eclampsia which was 1441.1±315.8, while in severe preeclampsia 1118.8±118.3, mild preeclampsia 902.4±102.5 and in normal pregnancy 769.3± 117.1. There was significant (p<0.05) positive correlation (ratio 0.880) between TAC measured by FRAP assay with severity of preeclampsia. Based on prevalence ratio with CI 95% subjects with FRAP level ≥ 769.3 had about 2.17 times higher risk to develop mild preeclampsia and subjects with FRAP level ≥ 769.3 had about 2.5 times higher risk to develop severe preeclampsia or eclampsia.Conclusion: There was a very strong positive correlation between TAC measured by FRAP assay with the severity of preeclampsia.[Indones J Obstet Gynecol 2011; 35-4: 155-60]Keywords: FRAP assay, total antioxidant capacity, preeclampsia - eclampsia

Perbedaan Indeks Apoptosis Plasenta Antara Preeklamsi dan Kehamilan Normal serta Hubungannya dengan Berat Badan Lahir dan Tekanan Darah Ibu

Majalah Kedokteran Bandung Vol 1, No 42 (2010)
Publisher : Majalah Kedokteran Bandung

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AbstrakAngka kejadian preeklamsi di Indonesia pada tahun 1980-2001 adalah 6-8% dari seluruh kehamilan, dengan angka kematian maternal 9,8-25%. Terdapat dugaan bahwa pada preeklamsi terjadi peningkatan apoptosis akibat turunnya ekspresi Bcl-2 sebelum usia kehamilan aterm. Tujuan penelitian ini untuk menentukan perbedaan antara indeks apoptosis (IA) plasenta pada preeklamsi dan kehamilan normal serta hubungannya dengan berat badan lahir bayi dantekanan darah ibu. Desain penelitian adalah observasional analitik secara potong silang, terhadap 63 subjek yang terdiri dari 32 ibu preeklamsi dan 31 ibu hamil normal. Penelitian dilakukan di Rumah Sakit Dr. Hasan Sadikin. Selama periode bulan April-Juni 2008, dilakukan pemeriksaan IA (caspase 3) secara imunohistokimia di laboratorium Patologi Anatomi. Analisis data ditentukan dengan menggunakan tes Chi kuadrat dengan p<0,05 sebagai batas kemaknaan. Pada kelompok preeklamsi terdapat peningkatan IA (21,9%), sebaliknya tidak terdapat peningkatan IA pada kehamilan normal (0%) (p=0,006). Terdapat perbedaan IA menurut berat badan lahir bayi yang bermakna pada preeklamsi (p=0,046), sedangkan pada kehamilan normal tidak bermakna (p>0,05). Tidak terdapat perbedaan IA menurut tekanan darah ibu pada kedua kelompok (p>0,05). Disimpulkan bahwa terdapat perbedaan indeks apoptosis antara preeklamsi dan kehamilan normal, sedangkan hubungannya dengan berat badan lahir hanya berbeda pada preeklamsi. [MKB. 2010;42(1):1-5].Difference of Placental Apoptosis Index in Preeclampsia and NormalPregnancy and Its Correlation with Birth Weight andMother´s Blood PressureThe incidence of preeclampsia in Indonesia (1980–2001) was around 6-8% of the total pregnancies with maternal mortality rate 9.8-25%. In preeclampsia, an increase of apoptosis occurs as a result of a decrease of Bcl-2 expression before a pregnancy reached full term. The objectives of this study were to determine placental apoptosis index (AI) inpreeclampsia and normal pregnancy, and its correlation with birth weight and mother´s blood pressure. This study was an observational analytical study using cross sectional design among 63 patients consisted of 32 preeclampsia and 31 normal pregnancies. This study was performed at Dr. Hasan Sadikin Hospital, during April-June 2008.Immunohistochemical analysis was used to examine the placental AI (caspase 3). Chi square test was used to analysed the result using p<0.05 for the significant difference. AI was significantly higher in preeclampsia group (21.9%) than in the normal pregnancies (0%) (p=0.006). There was significantly difference of AI with birth weight in preeclampsia with p=0.046. On the otherhand, there was no significantly difference of AI with birth weight in normal pregnancies. There was no significantly difference of AI with mother´s blood pressure in the two

Polimorfisme Gen MMP-9, Ekspresi MMP-9, dan Indeks Apoptosis Sel Serviks pada Kehamilan 21–36 Minggu

Majalah Kedokteran Bandung Vol 43, No 4 (2011)
Publisher : Fakultas Kedokteran, Universitas Padjadjaran

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Abstract

Ekspresi berlebihan serta aktivasi beberapa matriks metaloproteinase (MMP) sebelum persalinan mengarah pada degradasi jaringan amnion korion yang secara klinis disebut ketuban pecah dini (KPD). Peningkatan aktivitas MMP akibat polimorfisme gen matrik metaloproteinase-9 (MMP-9) (C-1562T) akan diikuti oleh apoptosis. Penelitian ini bertujuan mencari perbedaan ekspresi MMP-9, indeks apoptosis (IA) sel serviks, serta polimorfisme gen MMP-9 (C-1562T) pada kehamilan 21–36 minggu tanpa dan disertai KPD. Penelitian ini merupakan penelitian kasus kontrol yang dilakukan di RS Dr. Hasan Sadikin dan RS jejaring Bandung (Mei−November 2009). Tidak terdapat hubungan bermakna antara ekspresi MMP-9 dan IA sel serviks dengan setiap variabel pada kedua kelompok. Tiga kasus KPD ditemukan pada ibu berusia di bawah 20 tahun. Usia kehamilan 28−34 minggu memiliki peluang mengalami KPD lebih besar dibandingkan dengan usia kehamilan 21−28 minggu. Ibu hamil dengan indeks massa tubuh (IMT) 19–26 memiliki risiko mengalami KPD. Hanya terdapat satu sampel yang menunjukkan polimorfisme MMP-9 (C-1562T) di kelompok kehamilan 21–36 minggu disertai KPD. Dapat disimpulkan, tidak ditemukan hubungan bermakna antara ekspresi MMP-9 dan IA sel serviks pada kedua kelompok penelitian, begitu pula dengan polimorfisme MMP-9 (C-1562T) yang dapat menyebabkan peningkatan ekspresi MMP-9. [MKB.2011;43(4):199–206].Kata kunci: Apoptosis sel serviks, ekspresi MMP-9, ketuban pecah dini, polimorfisme gen MMP-9MMP-9 Gene Polymorphism, MMP-9 Expression and Cervical Apoptotic Index on 21–36 Weeks of PregnancyOver expression and premature activation of matrix metalloproteinase (MMP) can lead to degradation of amnion chorionic membrane which clinically called premature rupture of membrane (PROM). Increasing MMP activity caused by matrix metalloproteinase-9 (MMP-9) gene polymorphism (C-1562T) will be followed by apoptosis. This study was aimed to find the differences between MMP-9 expression and cervical apoptotic index (AI) and also MMP-9 (C-1562T) polymorphism on 21–36 weeks of pregnancy with or without PROM. This was case control study and conducted in Dr. Hasan Sadikin Hospital and Bandung Networking Hospitals (May−November 2009). There were no significant correlation between MMP-9 expression and cervical AI in every variable on both groups. Three cases of PROM were found in mothers below 20 years of age. Women with 28−34 weeks of pregnancy had a greater risk for PROM than 21−28 weeks. Pregnant women with body mass index (BMI) 19−26, had risk to have PROM. Only one sample that showed a MMP-9 (C-1562T) polymorphism in the premature labor with PROM group. It can be concluded that there are no significant correlation between MMP-9 expression and cervical cells AI on both groups as well as MMP-9 (C-1562T) polymorphism which can alter MMP-9 expression. [MKB. 2011;43(4):199–206].Key words: Cervical cell apopt

KORELASI ANTARA EKSPRESI AROMATASE, FOLLICLE STIMULATING HORMONE (FSH), DAN INSULIN-LIKE GROWTH FACTOR (IGF)-I DENGAN MATURITAS OOSIT PADA PELAYANAN TEKNOLOGI REPRODUKSI BERBANTU (TRB)

Indonesian Journal of Applied Sciences Vol 2, No 1 (2012)
Publisher : Universitas Padjadjaran

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Abstract

Karakteristik biokimia cairan folikel disekitar oosit memainkan peran penting dalam menentukan kualitas oosit yang padaakhirnya dapat meningkatkan keberhasilan program TRB. Secara umum mekanisme maturitas oosit yang dipengaruhi oleh unsur kimia cairan folikel melibatkan IGF-I yang bekerja dengan melipatgandakan efek FSHR, lalu menginduksi peningkatan kadar cAMP intraseluler yang merupakan mediasi utama stimulasi FSH pada ekspresi aromatase. Penelitian ini merupakan studi analitik korelasional dengan pendekatan crossectional pada pasien yang mengikuti program TRB di Klinik Aster Rumah Sakit Dr. Hasan Sadikin Bandung dalam kurun waktu Januari 2011 sampai dengan Februari 2012. Analisis hasil dilakukan menggunakan uji Shapiro Wilk, uji Wilcoxon, uji Mann-Whitney, uji ANAVA, rank Spearman, dan uji chi-kuadrat. Kemaknaan hasil uji akan ditentukan berdasarkan nilai p < 0,05. Pada penelitian ini didapatkan perbandingan jumlah oosit matur dan imatur pada ekspresi FSH kuat menunjukkan ada perbedaan yang bermakna (p=0,03). Analisis hubungan antara ekspresi IGF-I dengan maturitas oosit menunjukkan hasil yang tidak bermakna secara statistik (p=0,192). Didapatkan pula median proporsi prevalensimaturitas sebesar 0,71. Maturitas oosit dengan median rasio prevalensi > 0,71 pada ekspresi FSH kuat besarnya 1,71 kali bila dibandingkan dengan ekspresi FSH negatif. Pada ekspresi aromatase sedang dengan median rasio prevalensi maturitas > 0,71 sebesar 1,55 kali bila dibandingkan dengan ekspresi aromatase yang negatif.

The status of probiotics supplementation during pregnancy

Medical Journal of Indonesia Vol 24, No 2 (2015): June
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

Probiotics have been known for their use in medical field for quite a long time. Strong evidences are now available for the use of probiotics in clinical setting. One of the current issues on this topic is the use of probiotics in pregnancy. Recent studies showed that probiotics may be safe and beneficial for prenatal supplementation. In this review, we highlighted several proven use of probiotics supplementation in pregnant women. A few selected strains of probiotics showed promising outcome to prevent preterm labor and preeclampsia, and to reduce atopic eczema but not asthma and wheezing, in offspring of women who had prenatal probiotics supplementation. The mechanism of action responsible for this effect is closely related to the regulation of T cells, although the exact pathways are not defined yet.

IL-12 PE, CD 69 PERCP, CD3 FITC, AND CD4 APC OPTIMIZATION WITH ACTIVATION OF ISOLATED AGENT HEAT-KILLED SONICATED MYCOBACTERIUM TUBERCULOSIS BEIJING STRAIN

Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 4, No 2 (2014): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Infection caused by Mycobaterium tuberculosis exists in form of intracellular infection, which leads to lymphocyte activation. CD69 is the first lymphocyte activation marker expressed in Th1 lymphocyte, which follows by IL-12 release. Flow cytometry analysis can identify the subpopulations of lymphocytes and  intracellular cytokines such as IL-12, yet precise preparation needs to be done. This research aims to conduct optimization with four color lyse/wash flow cytometry assay system FastImmune™ FACSCalibur examination, with monoclonal antibody IL-12, CD69, CD3, and CD4 in succession uses fluorochrome PE, PerCP, FITC, and APC.To activate the lymphocytes from heparinized whole blood, we used activation agent which derives from isolated heat-killed sonicated Mycobacterium tuberculosis Beijing strain. Optimal concentration from the according activation agents is 40 mL. To determine the compensation, BDTM CompBead and blank-cell unstainning are used, but the maximum result showed by blank-cell unstainning.Each monoclonal antibody dosage of IL-12PE, CD69 PerCP, and CD3 FITC is 40 mL, while CD4 APC 5 mL. Total event lymphocyte is determined minimally by 10,000 events. With 18,510 total events and Th gated events quantity are 4,692, the result obtained is IL12-PE has 7.4% gated (347 events); CD69+ perCP/CD3+ FITC 18.2% (850 events); and CD69+ perCP/CD4+ APC 3.9%.

Faktor Diri Mahasiswa yang Memengaruhi Kelulusan Uji Kompetensi D III Kebidanan di Poltekkes Kemenkes Palu

Poltekita: Jurnal Ilmu Kesehatan Vol 11, No 1 (2017)
Publisher : Poltekkes Kemenkes Palu

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Kelulusan uji kompetensi di Institusi pendidikan merupakan parameter penting untuk menilai keefektifan pembelajaran, ketersediaan, dan kualitas sarana dan prasarana, tenaga edukatif yang terlibat di dalamnya serta pencerminan usaha belajar mahasiswa. Penelitian ini bertujuan untuk mengeksplorasi faktor mahasiswa yang memengaruhi kelulusan pada uji kompetensi bidan dan memperoleh instrumen baku berdasarkan hasil studi eksploratif yang telah diuji validitas dan reliabilitas.        Desain penelitian yang dipergunakan adalah metode campuran (mixed methods) dengan strategi sequential exploratory. Hasil eksplorasi dilakukan analisis tematik dan untuk memperoleh instrumen yang valid dan reliable dilakukan analisis menggunakan rasch model. Subyek penelitian adalah 6 alumni yang tidak lulus uji kompetensi, 3 alumni yang lulus uji kompetensi, 3 dosen D III di jurusan kebidanan Poltekkes Kemenkes PaluHasil eksplorasi diperoleh faktor-faktor yang memengaruhi kelulusan mahasiswa pada uji kompetensi bidan dari diri mahasiswa yaitu minat dan motivasi belajar sangat rendah, kurang percaya diri dan ketelitian dalam mengisi lembar jawaban komputer, dan ketidakjujuran selama proses pendidikan.Hasil pengolahan menggunakan rasch model pada kode m6, m7 dam m5 dengan kriteria sangat sulit dan sulit disetujui. Bunyi masing-masing item kode m6 (+1,28) bunyi item “saya rajin berkunjung dan meminjam buku ke perpustakaan”. Kode m7 (+0,95 logit) “Alumni yang belum lulus uji kompetensi sebaiknya bimbingan belajar”. Kode m5 (+0,80 logit) dengan bunyi item pernyataan “saya kurang fokus belajar karena saya memiliki aktifitas lain (pacaran, jalan-jalan atau bermain dengan teman)”. Item ini tidak ada responden yang menyetujui. Pada kriteria mudah sekali disetujui terdapat dua item yaitu kode m4 dan m3 dengan bunyi item pernyataan m4 (-0,76)  “Saya membuat laporan target kompetensi fiktif karena hanya sebagai syarat untuk mendaftar ujian akhir program”.  Item m3 (-1,25 logit) “saya berharap ada bimbingan belajar persiapan uji kompetensi”. Kedua item ini semua resonden sangat mudah menyetujui.Simpulan, kelulusan mahasiswa pada uji kompetensi masih rendah hal ini dipengaruhi oleh rendahnya motivasi dan minat belajar mahasiswa, ketidak jujuran dalam membuat laporan dan belum ada bimbingan belajar khusus menghadapi uji kompetensi. Disarankan Dosen memotivasi mahasiswa belajar dan menfasilitasi mahasiswa menghadapi uji kompetensi. Kata kunci : mutu lulusan, uji kompetensi