Claude Mona Airin
Universitas Gadjah Mada

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LEVEL HORMON TRIIODOTITYRONINE DAN THYROKSIN SAAT ESTRUS DAN OVULASI PADA SAPI BALI Mona Airin, Claude; Purwono Putro, Prabowo; Astuti, Pudji; Baliarti, Endang; Sunaryanto, .; Yulianto, Didik
Jurnal Sain Veteriner Vol 29, No 1 (2011): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3410.827 KB) | DOI: 10.22146/jsv.289

Abstract

Hormon tiroid mengontrol pertumbuhan, diferensiasi dan metabolisme semua sel somatik jaringan. Level abnormal hormon tiroid akan menyebabkan infertilitas atau berkurangnya fungsi reproduksi. Hormon tiroid ini kemungkinanm empunyai peranan penting dalam pengaturan steroidogenesis dalam folikel ovarium. Penelitian ini bertujuan untuk mendeteksi level T3 dan T4 sapi bali pada saat estrus dan ovulasi. Sapi bali yang digunakan tiga atau empat ekor dengan umur yang seragam (dua tahun) dan dalam keadaan sehat. Pengamatan estrusdilakukan setiap hari sedangkan penentuan waktu ovulasi menggunakan USG. Sampel darah diambil dari vena jugularis pada saat estrus dan ovulasi. Hasil penelitian menunjuk level T3 1,46ng/dl±0,173 saat estrus dan 1,98ng/ml±0,41 saat ovulasi, level T4 10,43 ±0,442 saat estus dan 10,48±0,896 saat ovulasi sedangkan ratio T3/T4. Level T3, T4 dan ratioT3/T4 tidak berbeda signifikan antara waktu estrus dan ovulasi. Kesimpulan penelitian ini adalah bahwa hormon T3 dan T4 kemungkinan berpengaruh terhadap fungsi ovarium.Kata kunci: triiodothyrosin, estrus, sapi Bali, waktu ovulasi
PENGARUH PENAMBAHAN SERUM SAPI PADA MEDIA KULTUR TERHADAP KOMPETENSI MEIOSIS OOSITANJING DARI BERBAGAI STADIUM ESTRUS SECARA IN VITRO Heru Fibrianto, Yuda; Mona Airin, Claude; Anindito, Amrullah; Rachmawati, Nuraini
Jurnal Sain Veteriner Vol 29, No 1 (2011): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5838.235 KB) | DOI: 10.22146/jsv.286

Abstract

Reproduction technology achievement on caninew as fastd eveloped, with born of cloning and transgenic dog, unforfunately that's technology still used oocyte matured in vivo because canine oocyte matwation in vitroin this animaln ot developedy et and mafurationr ates till low. The present study was to investigated the effects of cow serum supplementation on meiotic resumption of canine oocytes from various estms stage. Ovary obtained from bitchesa tan-, pro-and di-estrus stage after ovariohysterectomizeadt clinic veterinarypracticeO. varyw ereplaced in 0.9o/op hysiologycal saline added 1% pennicilline-streptomycinw ith temperature 37'C and then broughtt o laboratoryO. vary washeds everatl imesu sedP BSa ndo ocytesw ere obtained by slicing ovariant issueintissueculturemedium(TCM)-l99 supplemented with 25mMHepes, 1%pennicillin-streptomycin. Selected cummulus oocyte complexes (COCs) were matured in TCM-199 as control and TCM-199 + 10% cow serum as treatment, on incubator with 38C, 5% CO2, and 5% O2. After 72 h incubation and Hoechst 33342 staining, the result shows that cow serum supplement have higher meiotic resumption significantly (P<0.05) in MI achievement han in control and oocyte from proestrusi rave higher meiotic competent(46.5%) comparing oocyte from anestrus(29,4%)s ignificantly(P<0.05) but not significantly(P>0.05) with diestrus stages(34.8%).Keywords: meiosis, oocyte, ovarium, cows serum
PHYSIOLOGICAL RESPONSE OF BLIGON BUCK TO TRANSPORTATION: RELATION TO LEVEL OF THYROID HORMONE Astuti, Pudji; -, Sarmin; Kusumawati, Asmarani; Mona Airin, Claude; Maheshwari, Hera; Sjahfirdi, Luthfiralda
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Transportated animals may subject to a variety of physical stimuli including metabolism, crowding,noise, handling, isolation, agitation, and extreme temperature .The aim of this study was to determine thechanges of serum T4 and T3 concentration, during animals transportation. Six adult Bligon buck with bodyweight ranging from 26-30 Kg were used in this study. Two weeks prior to the experiment, the animalswere given anthelmintic Albendazole to eliminate egg worm. All animals were fed standard diet in theirpen at 10% of their body weight per head daily and commercial concentrate also given everyday. Freshwater was provided ad libitum. All animals were transported around village for 16 hours starting from18.00 pm until 10.00 am in open small truck (3 x 2 m); eye contact each others would be possible. Bloodsamples were withdrawn from jugular vein using vacutainer tubes containing heparin into 1.5 mL glasstubes, then centrifuged at 500 g for 15 minutes. Plasma was collected to be stored at ?20° C. The bloodwere collected every 4 hours from 8 hours before transportation ( at 10.00 am, 14.00 pm and 18.00 pm)until the time of arriving after transportation at 10.00 am. Plasma was harvested and stored at ?20° Cuntil T3 and T4 concentrations were measured using ELISA method (enzyme linked immunosorbentassay) product DRG, Germany. The result showed that transportation of Bligon bucks for 16 hours havean affect on level of T4 only (P&lt;0.05) and not for T3 concentration (P&gt;0.05) due to physical stimuli such ascrowding, heat stress, noise, handling would be discarded so that the metabolic process was stable. Duringtransportation, decreasing of T4 levels indicated conversion of T4 to T3 to form active hormone.