KUSDIANTORO MOHAMAD
Departemen Anatomi Fisiologi dan Farmakologi, Fakultas Kedokteran Hewan, Jalan Agatis Institut Pertanian Bogor

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Number and Quality of Oocytes Collected from Heterotopic Autografted Mice Ovary after PMSG Induction NURBARIAH, .; DJUWITA, ITA; MOHAMAD, KUSDIANTORO; SUPRIATNA, IMAN
HAYATI Journal of Biosciences Vol 18, No 4 (2011): December 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (254.373 KB) | DOI: 10.4308/hjb.18.4.151

Abstract

Heterotopic grafting sites can be useful in producing oocytes for in vitro Fertilization, therefore, maximising the oocyte yield from the graft by gonadotrophin stimulation would be advantageous. The aim of this study was to investigate the number and quality of oocytes collected from heterotopic autografted ovary after Pregnant Mare Serum Gonadothropin (PMSG) induction. Graft recipients were treated either with or without PMSG stimulation 48 hours prior to graft collection. Ovarian tissue from four weeks old mice (DDY strain) were autotransplanted under the kidney capsule of the same ovariectomized mice and the oocytes were collected 21 days after autotransplantation. The results showed that the average number of oocytes collected from autografted ovaries without PMSG induction were 9.0. + 2.8 not significantly different with those received PMSG induction, 10.9 + 5.1. The percentage of matured and fertilized oocytes and the developed embryos from the autografted ovaries without PMSG induction were 52.4, 33.4, and 26.0%, respectively not significantly different with those received PMSG induction, 53.2, 35.1, and 29.9%, respectively. The number of oocytes and the capacity to matured, fertilized and developed were significantly lower (P < 0.05) compared to the superovulated nongrafted (control) ovaries. In conclusion, PMSG induction on the graft recipients did not significantly increase oocytes yield from grafted heterotopic ovaries. The number and quality of oocytes produced from the autografted ovaries were lower than the superovulated nongrafted ovaries, but still can be used for in vitro embryo production after sequential in vitro maturation and fertilization.
Allotransplantasi Testis Mencit Muda sebagai Upaya Preservasi Gonad In Vivo Prasetyaningtyas, Wahono Esthi; Mohamad, Kusdiantoro; Fahrudin, Mokhamad; Djuwita, Ita; Agungpriyono, Srihadi
Jurnal Ilmu Pertanian Indonesia Vol 12, No 1 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Cancer disease are not detected only at adult but also at young age. One therapy for cancer diseases is chemotherapy and radiation, that give a side effect of infertility in the gonad, therefore, it is necessary to preserve the gonad. Sperm collection from adult is easy but not from the young patients.Keyword: transpalantation, testis, mencit muda, spermatogenesis
Korelasi Antara Lama Kebuntingan, Bobot Lahir dan Jenis Kelamin Pedet Hasil Inseminasi Buatan pada Sapi Bali Prasojo, Gatot; Arifiantini, Iis; Mohamad, Kusdiantoro
Jurnal Veteriner Vol 11, No 1 (2010)
Publisher : Jurnal Veteriner

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Abstract

Purpose of the research was to study the correlation between the gestation period with sex and birthweight of new born calves as the result of artificial insemination (AI) in bali cattle. Data were collecteddirectly from AI technicians in some districts in the province of bali from 1997 to 2003. A total, of 10759inseminated cows and 799 of which were with completed data of, date of birth, birth weight and sex ofcalves. The data were collected from 3 districts, involving Gianyar (205 samples), Badung (221 samples)and Bangli (373 samples). Result showed that the gestation period of bali cows were 284.4±5.7 days andbirth weight of 18.4±1.6 kg. Sex ratios (male : female) of bali calves resulted from AI in 1997 to 2003 were1.2:1. There were positive correlation between gestation period and birth weight (P&gt;0.01) as well asbetween gestation period and sex of calves with a high correlation coefficient (0.075) which proved thatmale calves had longer gestation period than females.
The Comparison of One and Two Steps Equilibration in Vitrification Process on The Morphology and Viability of Mouse Blastocysts Djuwita, Ita; Sari, Faralinda; Mohamad, Kusdiantoro
Jurnal Veteriner Vol 11, No 3 (2010)
Publisher : Jurnal Veteriner

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Abstract

A study was conducted to compare the effect of one and two steps equilibration method of vitrificationon the morphology and viability of mouse blastocysts. Blastocysts were firstly exposed to modified PhosphateBuffered saline (mPBS) containing 1% Bovine Serum Albumin (BSA) proceeded by exposure in mPBSrespectively containing 0.25M sucrose (S) for 2 minutes . Blastocysts were then exposed for 2 minutesrespectively to mPS+0.5M S (one step method) or in mPBS+0.5M S+10% ethylene glycol (EG) (two stepmethod).. Blastocysts were then exposed in mPBS+0.5M S+30% EG for 60 second, loaded into 0.25 mlplastic straw, and exposed immediately in vapor of liquid nitrogen for 10 second before they were and thenplunged into liquid nitrogen. The blastocysts were reconstituted by diluting with mPBS+0.5M S followedby mPBS+0.25M S for each 3 min and washed in mPBS without sucrose. The viability of cells was assessedby fluorescent vital staining, by re-expansion for 24 hours in vitro culture, and by implantation into therecipient oviduct. The percentages of morphologically normal blastocysts following recovery fromvitrification were higher (p&lt;0.05) in one step equilibration than in those of two steps methods (89.6%. vs82.6%). The viability of blastocysts examined under light microscope after staining with biz-benzimidizepropidiumiodine and 24 hours in vitro culture in one step methods (64.0%; 57.8%) were higher (p&lt;0.05)compared with two steps methods (40.0%; 35.6%), respectively. The implantation rate of vitrifiedblastocysts (23.1%) was not significantly different to that of fresh blastocysts (33.4%). These resultsshowed that the one and two step equilibration methods are effective for vitrification and maintaining theviability of the mouse blastocysts.
Pertumbuhan dan Sekresi Protein Hasil Kultur Primer Sel-Sel Serebrum Anak Tikus (IN VITRO GROWTH AND PROTEIN SECRETION OF NEWBORN RAT CEREBRAL PRIMARY CELLS CULTURE) Djuwita, Ita; Riyacumala, Vivit; Mohamad, Kusdiantoro; Prasetyaningtijas, Wahono Esthi; ., Nurhidayat
Jurnal Veteriner Vol 13, No 2 (2012)
Publisher : Jurnal Veteriner

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Abstract

The objective of this study was to identify the proliferation and differentiation of mice cerebrum cellsand its protein product. Research has been conducted on in vitro growth of three days old rat cerebrum cellsin Dulbecco’s Modified Eagle Medium (DMEM) containing 10% Amino Acid Non Essential (AANE), 10%Fetal Bovine Serum (FBS), 3mM NaHCO3, 50 mg/ml gentamycin, and supplemented with and without 5mg/ml insulin, 10 mg/ml transferin, 5 mg/ml selenium (ITS). Culture was done in 5% CO2, then incubatedat 37oC until 90% confluent. Identification were done on cell proliferation and population doubling time(PDT), number of neuron and glial cells, length of axon and dendrit, and protein secretion. The number andlength of neuronal cells were calculated by using hemocytometer and eyepiece micrometer, respectivelly.Protein secreted into culture medium, designed as conditioned medium (CM) was analysized using sodiumdodecyl sulfate–polyacrilamide gel electrophoresis (SDS-PAGE) method. Quantitative data were analyzedusing statistical T-test on Minitab program. In vitro culture of rat cerebrum cells showed two types of cellsincluding nerve cells of bipolar and multipolar neurons and glial cells including astrocyte (the fibrous andprotoplasmic), oligodendrocyte, and microglia. Supplementation of ITS into the culture medium increasedthe cell proliferation rate (P&lt;0.05) with lower PDT, and quantitatively, the 30 kDa secreted protein asindicated by the higher intensity of the protein band.
Pemaparan Gelombang Elektromagnetik Telepon Genggam pada Mencit (Mus musculus albinus) Periode Awal Kebuntingan Maria, Vincentia; Mohamad, Kusdiantoro; Boediono, Arief
ACTA VETERINARIA INDONESIANA - Indonesian Veterinary Journal Vol 2, No 1 (2014): Januari 2014
Publisher : Bogor Agricultural University

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Abstract

Peningkatan penggunaan telepon genggam diiringi dengan peningkatan kepedulian masyarakatterhadap bidang kesehatan dan keamanan terkait emisi gelombang elektromagnetik yang berasaldari telepon genggam. Penelitian ini bertujuan mengetahui tingkat keamanan paparan gelombangelektromagnetik telepon genggam melalui pengamatan terhadap jumlah implantasi dan anak lahirdengan menggunakan mencit sebagai hewan model. Dua puluh empat ekor mencit betina disinkronisasidan dikawinkan dengan mencit jantan dengan perbandingan jantan dan betina 1:1 (single mating).Pemaparan dilakukan dengan gelombang berfrekuensi 900 MHz selama 7 hari pertama setelah mencitkawin. Mencit betina dibagi ke dalam empat kelompok berdasarkan lamanya waktu paparan. Waktupaparan adalah satu, dua, dan empat kali per hari dengan masing-masing lama paparan 15 menit untukkelompok pertama, kedua, dan ketiga, sementara kelompok keempat sebagai kelompok kontrol tidakdiberikan paparan. Hasil penelitian menunjukkan tidak adanya pengaruh nyata lama paparan gelombangelektromagnetik telepon genggam terhadap jumlah implantasi dan anak lahir dari induk yang terpapar(P&gt;0,05). Untuk seluruh kelompok, jumlah implantasi berkisar antara 8,66 sampai dengan 10,00 danjumlah anak lahir berkisar antara 10,00 sampai dengan 12,33; tidak berbeda nyata dengan nilai padakelompok kontrol. Berdasarkan hasil penelitian, lama paparan gelombang elektromagnetik yang berasaldari telepon genggam dalam penelitian ini masih berada dalam tingkat aman untuk mencit bunting.
Allotransplantasi Testis Mencit Muda sebagai Upaya Preservasi Gonad In Vivo Prasetyaningtyas, Wahono Esthi; Mohamad, Kusdiantoro; Fahrudin, Mokhamad; Djuwita, Ita; Agungpriyono, Srihadi
Jurnal Ilmu Pertanian Indonesia Vol 12, No 1 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.442 KB)

Abstract

Cancer disease are not detected only at adult but also at young age. One therapy for cancer diseases is chemotherapy and radiation, that give a side effect of infertility in the gonad, therefore, it is necessary to preserve the gonad. Sperm collection from adult is easy but not from the young patients.Keyword: transpalantation, testis, mencit muda, spermatogenesis
Biological Analysis of Leydig Cells-Conditioned Medium To Support Rat Bone Marrow Mesenchymal Stem Cells Differentiation Kaiin, Ekayanti Mulyawati; Prasetyaningtyas, Wahono Esthi; Mohamad, Kusdiantoro; Djuwita, Ita; Yusuf, Tuty Laswardi; Setiadi, Mohamad Agus
ANNALES BOGORIENSES Vol 22, No 1 (2018): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (457.602 KB) | DOI: 10.14203/ab.v22i1.328

Abstract

The developed Leydig cells-conditioned medium (LCM) contains bioactive materials secreted by Leydig cells in vitro.  LCM was used to evaluate the ability of bone marrow mesenchymal stem cells differentiation. Bone marrow mesenchymal stem cells (1x 106 cell/ml) were cultured in : 1) DMEM supplemented with 10% NBCS as a control (M), 2) M supplemented with 10 ng/ml testosterone; 3) M supplemented with 50%  LCM ; 4) M supplemented with 50% LCM and 2.5 IU/ml hCG. Bone marrow mesenchymal stem cells that were cultured with  LCM has a positive reaction (57.4%) to histochemistry staining 3β-HSD and produced 1.87 ng/ml testosterone. Supplementation of hCG to LCM  increased the positive number of Leydig cells and testosterone production by 74.6% and 12.33 ng/ml (P<0.05). It can be concluded that Leydig cells-conditioned medium can support differentiation of bone marrow mesenchymal stem cells into Leydig cells.