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Respons Imunoglobulin-G dan Imunoglobulin-M Mencit yang Diberi Ekstrak Methanol Alga Biru Hijau dan Diinfeksi Dengan Takizoit Simanjuntak, Sorta Basar Ida; Moeljopawiro, Sukarti; Artama, Wayan Tunas; Wahyuono, Subagus
Jurnal Veteriner Vol 12, No 4 (2011)
Publisher : Jurnal Veteriner

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Abstract

Toxoplasmosis is an infectious disease caused by Toxoplasma gondii. This disease could severelyaffect humans and animals. Up to now there has been no simple treatment to fight toxoplasmosis. Aprospective alternative treatment to overcome this problem is by increasing immunity of the body using animmunostimulant such as Spirulina platensis. The aims of this research were to observe the potency of S.platensis as an immunostimulant and to find the most potential fraction of S. Platensis that can increasethe responses of IgG and IgM antibodies againts toxoplasma. The responses of these antibodies weremeasured using ELISA method. The isolation of compounds from S. platensis using Preparative ThinLayer Chromatography (PTLC) found three fractions which were a top fraction (I), a middle fraction (II),and a lower fraction (III). Forty-eight mice used in this research were divided into four different groupswith 12 mice in each group and treated differently. The top, middle, and lower fractions of S. platensis wereadministered orally to three groups of mice respectively at dose of 3mg/ml for each mouse while the micein the fourth group were kept as untreated controls. The treatment was conducted for 14 days consecutivelyand on the next day, all mice, including the controls, were challenged with tachizoit. The effect of S.platensisfractions on the responses of IgG and IgM antibodies were then measured at various time intervals, i.e. day0 (before infection) and day 1, 2, and 3 after infection. The results showed that IgG response increased inthe day 0 (2.504 OD) and the day 3 after infection (2.608 OD) while IgM response increased in day 1 afterinfection (2.898 OD). In conclusion, S. platensis was an immunostimulant and the middle fraction (II) of S.Platensis was the most potential fraction to increase immunity againts toxoplasma .
THE INFLUENCES OF CHITOSAN FROM Penaeus monodon ON C-REACTIVE PROTEIN EXPRESSION IN AORTA AND CORONARY ARTERY OF SPRAGUE DAWLEY RATS BY HIGH FAT INDUCTION Isdadiyanto, Sri; Moeljopawiro, Sukarti; Puniawati, Nyoman; Wuryastuty, Hastari
JOURNAL OF COASTAL DEVELOPMENT Vol 16, No 1 (2012): Volume 16, Number 1, Year 2012
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

The objective of this study was to analyze the influences of chitosan on C-Reactive Protein expression in aorta and coronary artery of Sprague Dawley rats by high fat induction. The animals for this study were 20 adult male rats divided into four groups, i.e. group I as the control was fed with basal diet containing normal fat for 3 months, group II was fed diet containing high fat for 3 months, group III was fed diet containing high fat and given chitosan 180 mg per kg body weight per day orally in  2 ml aquadest for 3 months, group IV was fed diet containing high fat for 3 months and  after 1 month given chitosan 180 mg per kg body weight per day orally in 2 ml aquadest for 2 months. Each group consisted of five animals. After 90 days, the rats were necropsied and the hearts were collected to histopathological and immunohistochemical analysis by immunohistochemistry streptavidin-biotin method. C-Reactive Protein expression in aorta was negative. Chitosan was able to prevent atheroma plaque formation in coronary artery and CRP may involve in atherosclerosis.
OPTIMASI KONDISI FERMENTASI UNTUK PRODUKSI SELULOSA BAKTERI OLEH STRAIN SLK-1 DALAM MEDIA DASAR AIR KELAPA (Optimization Of Fermentation Conditions For The Production Of Bacterial Cellulose By Slk-1 Strain In Coconut Water Based Medium) Sarkono, Sarkono; Moeljopawiro, Sukarti; Setiaji, Bambang; Sembiring, Langkah
Prosiding Seminar Biologi Vol 9, No 1 (2012): Seminar Nasional IX Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Abstract

ABSTRAK   Tujuan penelitian ini adalah mengoptimasi kondisi fermentasi terbaik strain Bakteri Asam Asetat penghasil selulosa yaitu isolat SLK-1.  Strain ini diisolasi dari buah salak pada penelitian sebelumnya.  Hasil optimasi menunjukkan bahwa kondisi fermentasi optimum untuk pertumbuhan dan produksi selulosa pada isolat SLK-1  dicapai dengan sumber karbon gula pasir, sumber nitrogen ammonium sulfat, pH 7, suhu inkubasi 25°C dan metode fermentasi statis. Karakter struktur permukaan selulosa hasil fermentasi isolat SLK-1 dipengaruhi oleh metode fermentasi yang digunakan.  Metode fermentasi goyangan berpengaruh menurunkan produksi selulosa pada  isolat SLK-1 dan merubah struktur permukaan yaitu susunan mikrofibril lebih renggang dan membentuk gelembung.   Kata Kunci: bakteri asam asetat, optimasi, fermentasi, selulosa bakteri, penggoyangan
OPTIMASI KONDISI FERMENTASI UNTUK PRODUKSI SELULOSA BAKTERI OLEH STRAIN SLK-1 DALAM MEDIA DASAR AIR KELAPA (Optimization Of Fermentation Conditions For The Production Of Bacterial Cellulose By Slk-1 Strain In Coconut Water Based Medium) Sarkono, Sarkono; Moeljopawiro, Sukarti; Setiaji, Bambang; Sembiring, Langkah
Prosiding Seminar Biologi Vol 9, No 1 (2012): Seminar Nasional IX Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Abstract

ABSTRAK   Tujuan penelitian ini adalah mengoptimasi kondisi fermentasi terbaik strain Bakteri Asam Asetat penghasil selulosa yaitu isolat SLK-1.  Strain ini diisolasi dari buah salak pada penelitian sebelumnya.  Hasil optimasi menunjukkan bahwa kondisi fermentasi optimum untuk pertumbuhan dan produksi selulosa pada isolat SLK-1  dicapai dengan sumber karbon gula pasir, sumber nitrogen ammonium sulfat, pH 7, suhu inkubasi 25°C dan metode fermentasi statis. Karakter struktur permukaan selulosa hasil fermentasi isolat SLK-1 dipengaruhi oleh metode fermentasi yang digunakan.  Metode fermentasi goyangan berpengaruh menurunkan produksi selulosa pada  isolat SLK-1 dan merubah struktur permukaan yaitu susunan mikrofibril lebih renggang dan membentuk gelembung.   Kata Kunci: bakteri asam asetat, optimasi, fermentasi, selulosa bakteri, penggoyangan
Chitosan Mempertipis Dinding dan Memperbesar Diameter Lumen Arteri Koroner Tikus Putih yang Diberi Pakan Lemak Tinggi (CHITOSAN COULD THINEN WALL AND WIDEN LUMEN DIAMETER CORONARY ARTERY OF SPRAGUE DAWLEY RATS INDUCED BY HIGH FAT RATION) Isdadiyanto, Sri; Moeljopawiro, Sukarti; Puniawati, Nyoman; Wuryastuty, Hastari
Jurnal Veteriner Vol 14, No 3 (2013)
Publisher : Jurnal Veteriner

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Abstract

The objective of this study was to analyze the influence of chitosan on coronary artery wall thicknessand lumen diameter of Sprague Dawley rats induced by high fat ration. The animals for this study weretwenty adult male rats divided into four groups, i.e. group I as the control was fed with basal rationcontaining normal fat for 3 months, group II was fed ration containing high fat for three months, group IIIwas fed ration containing high fat and given chitosan 180 mg per kg body weight per day orally in 2 mLaquadest for 3 months, group IV was fed ration containing high fat for three months and after one monthgiven chitosan 180 mg per kg body weight per day orally in 2 mL aquadest for two months. Each groupconsisted of five animals. After 90 days, the rats were necropsied and the hearts were collected tohistopathological. The difference between treatments was statistical analysis by Anava, and continued byLowest Significant Difference Test to locate the difference. Normal fat ration did not influence coronaryartery wall thickness and coronary artery lumen diameter in rats. High fat ration increased coronaryartery wall thickness and chitosan could be decreased coronary artery wall thickness in rats. High fatration + chitosan that given simultaneously could be increased coronary artery lumen diameter in rats.Based on the result of this study, it was concluded that high fat ration was a major factor able to causeatherosclerosis and chitosan was able to prevent atheroma plaque formation.
Cytotoxicity of Buah Merah (Pandanus conoideus Lamk.) Extract on Breast Cancer Cell Line (T47D) Nuringtyas, Tri R; Pratama, Yoga; G, Galih; Wahyuono, Subagus; Moeljopawiro, Sukarti
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

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Abstract

Buah Merah (Pandanus conoideus Lamk.) has been extensively used to treat various diseases includingcancer. There are many varieties of buah merah and there was no scientifi c study comparing cytotoxicity ofdifferent varieties. The objective of this study was to investigate the cytotoxicity of three varieties of buah merahknown as Barugum, Maler and Yanggiru on breast cancer cell line (T47D). All samples were collected fromPapua, Indonesia. Each sample was extracted consecutively using three solvents chloroform, methanol andwater resulted to nine crude extracts. The cytotoxic activities were determined using MTT assay. The crudeextract showed the lowest IC50 was selected for further bioassay-guided fractionation. Fractionation was doneusing vacuum liquid chromatography coupled with preparative TLC to fi nd the active compounds. Severaldetection reagents were applied to TLC for identifi cation of the class of the potent compounds. The resultshowed that the potent extracts was obtained from Barugum methanol extract followed by Maler chloroformextract with IC50 value of 132.83 μg/ml and 139.72 μg/ml, respectively. All Yanggiru extracts did not showactivity. The bioassay-guided fractionation of Barugum and Maler extracts showed that the most potent fractioneluted by a mixture of hexane:ethyl acetate (75:25), was in Maler variety with IC50 value of 25,7 μg/ml, fourtimes higher than the most potent fraction of Barugum with IC50 value of 104,61 μg/ml. TLC analysis of themost potent fraction showed that the active compounds was class of terpene. Result of this study supportedthe utilization of buah merah Maler variety for breast cancer treatment.
Cloning of cDNA Encoding GRA1 Protein of Tachyzoite Toxoplasma Gondii Local Isolate Sulistyaningsih, Erma; Moeljopawiro, Sukarti; Subandono, Jarot; Artama, Wayan T.
Indonesian Journal of Biotechnology Vol 10, No 1 (2005)
Publisher : Universitas Gadjah Mada

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Abstract

Gene encoding GRA1 protein is potent DNA-vaccine candidate against toxoplasmosis. The aim of the researchwas to clone the gene encoding GRA1 protein of tachyzoite Toxoplasma gondii local isolate by DNA recombinanttechnology. Tachyzoite was grown in Balb/c mice in vivo. Messenger RNA was isolated from total RNA and itwas used to synthesis cDNA. Complementary DNA encoding GRA1 protein of tachyzoite Toxoplasma gondii localisolate was amplified and cloned in a prokaryote cloning vector. The recombinant GRA1-encoding gene was thendigesting using EcoRI restriction endonuclease and sequencing. The result showed that the recombinant GRA1-encoding gene consisted of DNA sequences encoding all signal peptide and mature peptide of GRA1 protein.Alignment of recombinant GRA1 sequence to gene encoding GRA1 protein of Toxoplasma gondii RH isolate showed100% homologous.Keywords: GRA1 protein, Toxoplasma gondii, tachyzoite, cloning, cDNA
EFEK POLIMORFISME GENA NITRIT OKSIDA SINTASE3(NOS3) TERHADAP KADAR NITRIT OKSIDA DAN TEKANAN DARAH PADA INDIVIDU TERPAPAR PLUMBUM Hernayanti, Hernayanti; Moeljopawiro, Sukarti; Sadewa, Ahmad Hamim; Hariono, Bambang; Wahyuono, Subagus
Jurnal Manusia dan Lingkungan (Journal of People and Environment) Vol 19, No 2 (2012)
Publisher : Pusat Studi Lingkungan Hidup Universitas Gadjah Mada

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Abstract

Penelitian ini bertujuan untuk mengidentifikasi efek polimorfisme gena nitrit oksida sintase3 terhadap kadar nitrit oks ida (NO) dan tekanan darah pada individu terpapar Plumbum. Metode penelitian menggunakan metode survai dengan rancangan kasus kontrol. Subjek kasus terdiri dari 30 orang pekerja bengkel mobil dan 30 orang subjek kontrol berasal dari pedesaan yang mewakili area yang tidak terpolusi Pb.Genotip individu ditentukan dengan metode PCR~RFLP. Parameter yang diukur adalah kadar NO, tekanan darah sistolik dan diastolik serta kadar Pb. Data dianalisis menggunakan uji t independent. Hasil penelitian menunjukkan bahwa 40% dari subjek kasus, terdeteksi sebagai individu pembawa polimorfisme gena NOS3 dengan genotip GA, sedangkan 60% dari subjek kasus dan subjek kontrol terdeteksi sebagai individu nonpolimorfisme gena NOS3 dengan genotip GG. Hasil uji t menunjukkan untuk parameter NO, tekanan sistolik, diastole serta Pb menunjukkan perbedaan yang sangat nyata an tara individu pembawa polimorfisme gena NOS3 dengan individu nonpolimorfisme. Kadar NO individu pembawa polimorfisme NOS3 lebih rendah dibandingkan individu nonpolimorfism. Sebaliknya kadar Pb, tekanan sistolik dan diastole individu pembawa polimorfisme gena NOS3 lebih tinggi dibandingkan individu nonpolimorfisme. Kesimpulan yang diperoleh adalah adanya polimorfisme gena NOS3 dan paparan Pb menyebabkan ketersediaan NO makin rendah dan meningkatkan kadar Pb, tekanan sistolik dan diastolik. Individu terpapar Pb pembawa polimorfisme gena NOS3 beresiko mengalami penyakit hipertensi yang lebih parah dibandingkan individu nonpolimorfisme terpapar Pb.
Indeks sporozoit Anopheles spp. (Culicidae: Anophelinae) di daerah endemis malaria di Kecamatan Kokap, Kabupaten Kulon Progo Andiyatu, Andiyatu; Soesilohadi, R.C. Hidayat; Handayani, Niken Satuti Nur; Moeljopawiro, Sukarti
Jurnal Entomologi Indonesia Vol 13, No 2 (2016): July
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (449.112 KB) | DOI: 10.5994/jei.13.2.63

Abstract

Perbedaan endemisitas malaria antar desa dalam kecamatan yang sama mungkin berkaitan dengan perbedaan infektivitas dari Anopheles sp. Tujuan penelitian ini ialah untuk memeriksa proporsi spesies yang positif sporozoit atau infektivitas spesies (IS) dan proporsi sampel yang positif sporozoit atau indeks sporosoit total (IST) dari sebuah desa endemik tinggi (DET) dan sebuah desa endemik rendah (DER) di Kecamatan Kokap, Kabupaten Kulon Progro, Jawa Tengah. Empat spesies nyamuk Anopheles diperiksa, diantaranya Anopheles vagus Donitz, Anopheles maculatus (Theobald), Anopheles balabacensis Baisan, dan Anopheles aconitus Donitz. Nyamuk Anopheles sp. dikumpulkan secara serentak di dua desa, masing-masing lima kali selama Oktober–Desember 2013, dengan interval dua minggu, menggunakan metode resting collection. Pengumpulan nyamuk dilakukan setiap jam (@ 50 menit) di tiga rumah oleh dua kolektor per rumah (satu di dalam dan satu di luar), dari jam 18:00 sampai jam 06:00. Nyamuk betina parous diperiksa menggunakan metode Multiplex-PCR untuk mendeteksi keberadaan sporozoit. Pemeriksaan 77 sampel DNA menunjukkan bahwa IS dan IST kedua desa tersebut (49 DET dan 28 DER) berbeda nyata dengan rasio IS 66,7% : 33,3% dan rasio IST 20,41% : 3,57% (OR = 6,9; CI95% = 0,87 sampai 57,29; p = 0,021). Temuan ini mengindikasikan bahwa suatu transmisi malaria intensitas tinggi masih memungkinkan timbul di DET dan karenanya dibutuhkan suatu tindakan pengendalian vektor yang spesifik wilayah.
Kariotip Kromosom Salak Parjanto, ,; Moeljopawiro, Sukarti; Artama, W. T.; Purwantoro, Aziz
Zuriat Vol 14, No 2 (2003)
Publisher : Zuriat

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Abstract

Penelitian sitogenetika tanaman salak (Salacca zalacca [Gaertner] Voss) merupakan aspek penting dalam analisis genetika tanaman salak dan menunjang program pemuliaan untuk merakit kultivar salak unggul. Analisis kariotip kromosom salak telah dilakukan dengan metode squash dan pewarnaan acetoorcein. Dengan metode ini, pengamatan morfologi kromosom, yakni panjang dan bentuk kromosom, dapat dilakukan dengan hasil baik pada stadia prometafase. Hasil pengamatan menunjukkan bahwa panjang kromosom salak berkisar 1.15 µm–2.38 µm. Rumus kariotip salak adalah 2n = 28 = 11 m + 1 m (SAT) + 2 sm, yakni terdiri atas 11 pasang kromosom metasentris, 1 pasang kromosom metasentris dengan satelit kromosom, dan 2 pasang kromosom submetasentris. Indeks asimetri kariotip intrakromosomal adalah 0.3 ± 0.03, indeks asimetri inter kromosomal adalah 0.2 ± 0.02. Beberapa pasangan kromosom salak mempunyai bentuk dan ukuran yang mirip sehingga sulit dibedakan. Identifikasi kromosom salak dengan teknik chromosome banding perlu dilakukan untuk mendukung hasil yang telah dicapai.