SUGIONO MOELJOPAWIRO
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar No. 3A, Bogor 16111 Telp. (0251) 8337975

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Inheritance of Blast Resistance (Pyricularia grisea Sacc.) on Interspecific Crossing between IR64 and Oryza rufipogon Griff UTAMI, DWINITA WIKAN; ASWIDINNOOR, HAJRIAL; MOELJOPAWIRO, SUGIONO; HANARIDA, IDA; REFLINUR, REFLINUR
HAYATI Journal of Biosciences Vol 13, No 3 (2006): September 2006
Publisher : Bogor Agricultural University, Indonesia

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Abstract

Blast disease affected by Pyricularia grisea causes high percentage of yield losses in rice production. The improvement of durable Blast resistance is difficult due to the complexity of the inheritance of this trait. This study was conducted to evaluate the genetic control and inheritance of Blast resistance trait in interspesific population between IR 64 (accepted Indonesian rice type, medium resistant to Indonesian Blast pathogen) and Oryza rufipogon (AA genome; acc. No.IRGC#105491; donor for Blast resistance). Six populations, i.e. P1, P2, F1, F2, BCP1, and BCP2, were inoculated with three Indonesian races of Blast pathogen. The disease intensity was determined on the basis of disease leaf area (DLA). The three types of gene interactions (additive, dominance, and epistatis) were significantly involved in this trait. Among the digenic epistasis interactions, all of the additive x additive [i], additive x dominance (j) and dominance x dominance (l) contributed to the trait. Broad-sense heritability ranged from 50.30 up to 91.20%, while the narrow heritability ranged from 16.98 up to 73.20%. The presence of additive gene effect indicated that selection of this trait is promising. Key words: inheritance, Blast resistance, wild rice, Oryza rufipogon
Evaluasi Beberapa Galur-Pup1Tanaman Padi (Oryza sativaL.) pada Larutan Hara dan Lapangan Prasetiyono, Joko; Suhartini, Tintin; Soemantri, Ida Hanarida; Tasliah, ,; Moeljopawiro, Sugiono; Aswidinnoor, Hajrial; Sopandie, Didy; Bustamam, Masdiar
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol 40, No 2 (2012): JURNAL AGONOMI INDONESIA
Publisher : Bogor Agricultural University

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Phosphorus (P) deficiency is one of major problems in rice cultivation. Development of a tolerant variety to P deficient soil is expected to reduce the needs of P fertilizer. The aim of this research was to evaluate the agronomic performance of Pup1-introgression rice lines. This research consisted of two separate experiments, an evaluation on rice grown in nutrient solution in the greenhouse of ICABIOGRAD, Bogor; and field evaluation at upland rice screening site Taman Bogo, Lampung. The rice lines used were BC2F3 derived from crossing of Situ Bagendit with Kasalath and NIL-C443, and from crossing of Batur with Kasalath and NIL-C443. Molecular analysis was conducted to verifiy the Pup1 introgression in the lines used and successfully confirmed the occurance of homozygote Pup1 segment in those lines. Evaluation of rice lines grown in nutrient solution was performed in a split plot factorial design, using the dose of Al (0 and 45 ppm Al3+) as main plots and dose of P (0.5 and 10 ppm P) as subplots. Field evaluation was performed in split plot design, with P fertilization (0 and 500 kg SP-18 ha-1) as main plots and BC2F3 lines as the subplots. Evaluation using Yoshida nutrient solution showed that the BC2F3 lines had greater total dry weight under low-P condition (37.5-112.5%), especially under Al-toxicity, compared to the respective parental varieties (Situ Bagendit and Batur). Field evaluation showed that the Pup1-introgression lines had greater shoot dry weight than the respective parental lines (10.5-74.82%). However there was no significnant effect of Pup1 introgression in terms of weight of filled grain. Keywords: Al toxicity, BC2F3-Pup1 lines, P-deficiency, rice, Yoshida nutrient solution
Kriteria seleksi berdasarkan sifat morfologi tanaman kopi robusta Alnopri, Alnopri; Setiamihardja, Ridwan; Moeljopawiro, Sugiono; Hermiati, Nani
Zuriat Vol 3, No 1 (1992)
Publisher : Zuriat

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Suatu penelitian telah dilaksanakan di kebun percobaan Pusat Penelitian Perkebunan Kaliwing, Jember, dari bulan Juni 1989 sampai dengan September 1989. Tujuan penelitian adalah untuk mengetahui hubungan sifat-sifat morfologi dengan daya hasil dan nilai duga daya waris sifat0sifat tersebut. Bahan penelitian adalah 24 koln kopi Robusta yang ditanaman pada bulan Desember 1973, dan dipelihara menurut sistem batang tunggal. Tata letak percobaan menggunakan rancangan acak kelompok dengan tiga ulangan.hasil penelitian menunjukkan bahwa sifat rata-rata jumlah buku produktif dan bobot 100 butir biji mempunyai hubungan positif sangat nyata dengan daya hasil, sehingga sifat-sifat tersebut dapat dijadikan sebagai kriteria seleksi.Nilai duga daya waris (heritabilitas dalam arti luas) sifat rata-rata jumlah buku produktif rendemen hasil, dan bobot 100 butir biji adalah tinggi, sehingga mudah diwariskan dan seleksi dapat dilakukan pada generasi awal.
APLIKASI TEKNIK MARKER ASSISTED SELECTION (MAS) DALAM SELEKSI INTROGRESI GENOTIP TAHAN PATOGEN BLAS DARI Oryza rufipogon KE DALAM IR 64 Utami, Dwinita W.; Aswidinnoor, Hajrial; Setiawan, Asep; Moeljopawiro, Sugiono; Guhardja, Edi
Zuriat Vol 15, No 2 (2004)
Publisher : Zuriat

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Perakitan varietas unggul yang memiliki sifat ketahanan terhadap pathogen blas (Pyricularia grisea) merupakan salah satu program utama dalam pemuliaan padi. Untuk mendukung program tersebut diperlukan gene pool yang luas terutama untuk trait kuantitatif. Salah satu strategi yang dapat ditempuh adalah dengan mengidentifikasi lokus kuantitatif (QTL) yang terdapat pada spesies liar dan mentransfernya ke dalam kultivar padi. Spesies padi liar Oryza rufipogon diidentifikasi mempunyai lokus ketahanan kuantitatif (QRL) terhadap patogen blas. Pemanfaatan marka molekuler dalam pemuliaan tanaman salah satunya adalah untuk mempercepat seleksi (MAS) kembalinya genom tetua pemulih dalam persilangan silang balik. Dalam rangka menseleksi progeni persilangan O. rufipogon dengan IR64 menggunakan marka molekuler, dalam penelitian ini dilakukan seleksi introgresi genotip tahan yang hanya sedikit membawa fragmen dari tanaman padi liar. Tujuan dari penelitian ini adalah menseleksi genotip tahan blas dan menganalisis introgresi dan keterpautannya dengan tentative QTL tahan blas. Hasil analisis menunjukkan terdapat 20 genotip tahan dengan tingkat introgresi yang bervariasi dari 9%–40% dari total kromosom yang terpetakan. Analisis keterpautan menunjukkan bahwa genotip nomor 118 mempunyai QTL tahan blas pada kromosom 2, genotip nomor 337 mempunyai QTL tahan blas pada kromosom 12 dan genotip nomor 343 mempunyai QTL tahan blas pada kromosom nomor 9. Nomor-nomor genotip yang telah terseleksi tingkat ketahanan dan introgresinya dapat mempercepat kembalinya genome tetua pemulih, sebagai bahan untuk pembentukan populasi lebih lanjut.
Pemetaan QTL Resistensi Tanaman Jagung terhadap Penyakit Bulai Di Bogor Azrai, M.; K., Murdaningsih H.; Rostini, Neni; Moeljopawiro, Sugiono; Ruswandi, D.
Zuriat Vol 13, No 2 (2002)
Publisher : Zuriat

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Sebagai bagian dari suatu penelitian kolaboratif AMBIONET (Asian Maize Biotechnology Network), Indonesia turut serta dalam kegiatan marker assisted selection (MAS) untuk pemuliaan jagung tahan bulai. Tujuan penelitian adalah untuk mengidentifikasi dan memetakan 146 marka RFLP (Restriction Fragment Length Polymorphism) yang berasosiasi dengan lokus karakter kuantitatif ketahanan dari 134 populasi genotip RIL (recombinant inbred lines) progeni CML 139 x Ki3. Penelitian dibagi atas dua tahap yaitu tahap analisis molekuler dan tahap pengujian di lapangan. Analisis molekuler memanfaatkan data dari Pusat Penelitian Internasional Jagung dan Terigu (CIMMYT), Mexico. Penelitian lapangan dilakukan di Bogor pada bulan Mei 2002 sampai Juli 2002. Data fenotipik (persentase serangan bulai) dianalisis dengan menggunakan program alpha lattice, sedangkan analisis QTL (Quantitative Trait Loci) menggunakan “software computer” program “Windows QTL Cartographer V.1.21”. Hasil analisis QTL menunjukkan bahwa dari 146 marka RFLP pada 134 populasi genotip RIL progeni CML 139 x Ki3, terdapat 14 marka yang terdistribusi pada enam kromosom dari 10 kromosom berdasarkan fenotaiping di Bogor. Efek aditif yang terdeteksi dalam analisis QTL pada umumnya bernilai negatif yang mengarah ke CML 139 (genotip peka).
Marka Mikrosatelit Sebagai Alternatif Uji BUSS dalam Perlindungan Varietas Tanaman Padi Moeljopawiro, Sugiono
Zuriat Vol 18, No 2 (2007)
Publisher : Zuriat

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Studi dilaksanakan di BB Biogen untuk menjajagi kemungkinan penggunaan SSR bagi BUSS pada padi. Varietas Fatmawati dan tujuh varietas pembanding diuji dengan 10 marker SSR. Diperoleh 125 lokus pada kromosom 1, 2, 5, 6, 7, 10 dan 11 dengan jumlah alil berkisar antar 6 pada BPC samapi 33 pada Fatmawati. Marka mikrosatelit dapat digunakan untuk mendeteksi perbedaan antar varietas maupun untuk mencirikan varietas, dimana Marka RM11 merupakan marka utama yang terdapat pada varietas Fatmawati, Maros, Barumun, Gilirang dan Memberamo, RM237 pada Cisadane dan BP630, dan marka RM133 dan RM287 pada BPC. Baik secara morfologi maupun secara molekuler terbukti bahwa kedelapan varietas yang diuji masih belum seragam. Khusus untuk varietas Fatmawati yang dijadikan varietas simulasi uji BUSS, hasil tersebut menunjukkan bahwa bahwa varietas Fatmawati keragamannya masih tinggi sehingga tidak dapat diberi perlindungan PVT dan belum layak dilepas sebagai varietas unggul baru, karena masih belum seragam. Atas dasar pertimbangan waktu, biaya dan ketelitian, marka mikrosatelit dapat diusulkan sebagai alternative uji BUSS di masa depan.
Identifikasi Marka Polimorfik untuk Pemuliaan Padi Toleran Defisiensi Fosfor Prasetiyono, Joko; Aswidinoor, Hajrial; Moeljopawiro, Sugiono; Sopandie, Didy; Bustamam, Masdiar
Jurnal AgroBiogen Vol 4, No 2 (2008): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

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Information on polymorphismsamong rice parents are very important in ricebreeding for tolerance to phosphorus defficiency. A studywas conducted at the Molecular Biology Laboratory,Indonesian Center Agricultural Biotechnology and GeneticResources (ICABIOGRAD) from October 2006 to July 2007 toidentify polymorphism markers from 6 rice genotypes. Therice genotypes, i.e., Dodokan, Situ Bagendit, Batur, Kasalath,NIL-C443, dan K36-5-1-1 were analyzed for polymorphismsusing 496 SSR markers, which cover the rice genomes.Seven of the 496 markers were used as foreground andrecombinant selection markers, and the rests (489 markers)were used as background selection markers. PCR amplificationswere separated on a 5% polyacrylamide gel andcolored by the silver staining method. Three different markersamong the seven foreground and recombinant selectionmarkers were selected from each crossing, which aretightly linked with Pup1 gene and have a distance less than 5cM. These markers are Dodokan vs Kasalath (RM277, SSR3,RM519), Dodokan vs NIL-C443 (RM277, SSR3, RM519),Dodokan vs K36-5-1-1 (RM277, SSR3, RM519), Situ Bagenditvs Kasalath (RM28102, SSR3, RM519), Situ Bagendit vs NILC443(RM28102, SSR3, RM519), Situ Bagendit vs K36-5-1-1(RM511, SSR3, RM519), Batur vs Kasalath (RM277, RM1261,RM519), Batur vs NIL-C443 (RM277, RM1261, RM519), andBatur vs K36-5-1-1 (RM28102, SSR3). Variations in backgroundselection primers were found in each chromosomeand in each parent combinations. Primers on chromosome4, 5, and 12 showed the lowest polymorphisms; moreprimers are needed for these chromosomes.
Analisis Regulasi dan Kebijakan Keamanan Hayati dan Peluang Keberhasilan Adopsi Benih Transgenik di Indonesia Saragih, Edwin S; Sitorus, Santun RP; Harianto, Harianto; Moeljopawiro, Sugiono
Jurnal AgroBiogen Vol 6, No 1 (2010): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

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Analysis of Regulation and Policy on Biosafety andLikelihood of Transgenic Seeds Adoption in Indonesia.Edwin S. Saragih, Santun R.P. Sitorus, Harianto, andSugiono Moeljopawiro. Since more than 10 years, anumber of works in field of modern biotechnology havebeen programmed in public research institutes anduniversities in Indonesia and few foreign companies haveput efforts in introducing transgenic varieties. This significantdevelopment raises intriguing question as to why there hasnot been any transgenic food crop seed planted by farmersin the country. A status quo was observed in whichregulatory regime on biosafety has been in a situation ofprolonged transitional phase and necessary institutionalframework has not been firmly in place. There weredistinguished lines among stakeholders on benefitawareness, risks perception and worry on multinationalcompanies’ control over seed supply. There is a fairexpectation that similar benefits experienced by adoptingcountries could also help increase food production inIndonesia. However, potential contribution of transgenicseeds for the country is still largely unexplored. There arenumbers of potential transgenic seeds namely transgenicrice, soybean, potato, tomato and corn, with the latter wouldshow slightly better likelihood of success once adoptionhappens. Decision making instrument as determinant factorin ensuring safe application and release of transgenic seedshas not yet existed despite the fact that capacity for biosafetyassessment conduct is undoubtedly sufficient. It is importantto note that the new regulation on biosafety (PP No. 21/2005)open opportunities for assessing transgenic product under atransitional clause. Nonetheless, the new regulation has notbeen able securing food safety statement of importedtransgenic products (especially corn and soybean) whichhave been used for domestic consumption.