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Acid Resistance Test of Probiotic Isolated from Silage Forage Swamp on In Vitro Digestive Tract Sandi, Sofia; Miksusanti, Miksusanti; Sari, Meisji Liana; Sahara, Eli; Supriyadi, Adi; Gofar, Nuni; Asmak, Asmak
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 4, No 1 (2019): February 2019
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

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Abstract

Antibiotics are commonly used in poultry ration, and afterward these drug residues may persist in foods. There is an alternative source of antibiotics in poultry rations, one of which is using probiotics. Probiotics are living microorganisms capable of reaching the gastrointestinal tract and benefiting health, leaving no residue in the body. Probiotics can be originated from bacteria such as lactic acid bacteria (LAB) which produced lactic acid and antimicrobial components. LAB must be able to live in the digestive system with various pH condition. pH resistance testing of LAB was performed by introducing diluted bacteria into several buffer solutions with different pH and a predetermined incubation time, then was grown in MRS agar medium. This study was conducted to determine the resistance of LAB isolates as probiotics from silage forage swamp at different pH distributed in vitro digestant. The design used was a complete randomized design consist of 3 treatments and 3 replications. The treatments were using isolate of Kumpai Tembaga silage (P1), isolate of 50% Kumpai Tembaga silage and 50% Kemon Air silage (P2), and isolate of Kemon Air silage (P3). The results showed that all isolates used were lactic acid bacteria, and the treatment significantly affected the value of LAB resistance test against low pH and high pH. Isolate from Kumpai Tembaga silage (P1) is a lactic acid bacterium with higher ability to survive in pH of in vitro digestive system.
Mode of Action Temu Kunci (Kaempferia pandurata) Essential Oil on E. coli K1.1 Cell Determined by Leakage of Material Cell and Salt Tolerance Assays MIKSUSANTI, MIKSUSANTI; JENIE, BETTY SRI LAKSMI; PRIOSOERYANTO, BAMBANG PONTJO; SYARIEF, RIZAL; REKSO, GATOT TRIMULYADI
HAYATI Journal of Biosciences Vol 15, No 2 (2008): June 2008
Publisher : Bogor Agricultural University, Indonesia

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Abstract

The essential oil of Kaempferia pandurata consist of terpen and oxygenated terpen that exhibits broad-spectrum antimicrobial activity. It’s mode of action against the gram-negative bacterium E. coli K1.1 has been investigated using a range of treatments. The mode action of the essential oil were analyzed by it’s ability to leakage E. coli K1.1 cell, to change permeability of the cell, and to alter salt tolerance of the cell. Ion leakage from the cell were analyzed by atomic absorption spectrophotometer. Salt tolerance assays was conducted by investigating the ability of E. coli K1.1 treated with temu kunci essential oil to grow on NA supplemented with NaCl. Protein and acid nucleic leakage were analyzed by UV spectrophotometer. There were inorganic compound leakage (potassium, calcium ion) and organic compound leakage (nucleic acid, protein) from cytoplasmic membrane, after exposing this organism to essential oil of Kaempferia pandurata. The more concentration of oil added, the more leakage was observed due to the loss of absorbing material such as nucleic acid (260 nm) and protein (280 nm), the loss of potassium and calcium ion, and loss of the salt tolerance of E. coli K1.1. Key words: essential oil, E. coli K1.1, leakage, salt tolerance assay
Antibacterial activity of temu kunci tuber (kaempheria pandurata) essential oil against Bacillus cereus Miksusanti, Miksusanti; J, Betty S.L.; Syarief, Rizal; Pontjo, Bambang; Mulyadi, Gatot T.
Medical Journal of Indonesia Vol 18, No 1 (2009): January-March
Publisher : Faculty of Medicine Universitas Indonesia

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Abstract

Aim The mechanism of temu kunci tuber essential oil potential as antimicrobial agent has not been reported. To analyze the mechanism of antibacterial activity of temu kunci tuber essential oil from Yogyakarta on B.cereus. Antibacterial activity of essential oil were analyzed for its ability to disrupt bacterial cell membrane, that caused cell leakage and altered the morphology of the bacteria. Leakage was measured by analyzing the Ca+2, K+ ion outflow using an atomic adsorption spectrometry (AAS), and protein and nucleic acid using an ultraviolet spectrophotometer (UVS) on 280 nm and 260 nm respectively. Alterations in morphology were assessed using scanning electron microscopy (SEM).Results Minimum inhibitory concentration (MIC) of temu kunci essential oil on B.cereus was 0.12% (v/v). Treatment of B. cereus using 1MIC and 2MIC showed significant leakage compared to control (P<0.05). The K+ and Ca+2 ion leakage from the bacterial cells were between 10.32-35.57% and 15.05-41.54% respectively and showed significant difference compared to control (P<0.05). The absorbance observed by UVS for protein and nucleic acid leakage were 0.6330-0.8670 at 280 nm and 0.4320-0.8307 at 260 nm, respectively, and were significantly different compared to control (P<0.05). Exposure of 1 MIC temu kunci essential oil on B.cereus caused thickening as well as irregularities on the cell wall. At 2 MIC cells seemed transparent, empty looking and showed a ghost-like appearance.Conclusion Kaempheria pondurata essential oil could cause leakage and alter the morphology of the bacteria. (Med J Indones 2009; 18:10-7)Keywords: leakage, morphology
Studi Kandungan Nitrogen Oksida (NOx) pada Udara di Sepanjang Jalan Palembang-Prabumulih Yuliasari, Nova; Miksusanti, Miksusanti; Tridayona, Tridayona
Jurnal Penelitian Sains No 16 (2004)
Publisher : Faculty of Mathtmatics and Natural Sciences

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Abstract

Telah dilakukan penelitian kandungan gas NOx di udara sepanjang ruas jalan Palembang-Prabumulih dengan menggunakan metoda spektrofotometri. Cuplikan udara dihisap tabung impinger yang terhubung secara seri. Kadar NOx tertinggi terdapat pada lokasi Bundaran Air Mancur Palembang sebesar 83,26 mg/m3 (pagi); 91,22 mg/m3 (siang) dan 88,50 mg/m3 (sore). Sedangkan kadar Nox terendah terdapat pada Perumahan Bumi Inderalaya Permai sebesar 16,48 mg/m3 (pagi); 19,03 mg/m3 (siang) dan 17,51 mg/m3 (sore). Kadar Nox di sepanjang jalan Palembang-Inderalaya masih di bawah nilai ambang batas (92,50 mg/m3). Variasi kadar NOx secara umum disebabkan faktor kelambaban dan jumlah kendaraan. Temperatur dan tekanan udara relatif tidak mempengaruhi kadar gas NOx di udara.   
The Effect of Lactobacillus acidophilus and Chito−Oligosaccharide on Antibacterial Activity and Organic Acid Production Miksusanti, Miksusanti; Saputra, Harian; Sandi, Sofia; Hermansyah, Hermansyah
Indonesian Journal of Fundamental and Applied Chemistry Vol 1, No 2 (2016): June 2016
Publisher : Indonesian Journal of Fundamental and Applied Chemistry

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Abstract

The effect of Lactobacillus acidophilus combined with and without Chito-Oligosaccharide (COS) on the growth of Escherichia coli and Staphylococcus aureus had been studied. The antibacterial activity of L. acidophilus before and after combination with COS 0.2% was tested on bacteria of E. coli and S. aureus with well-diffusion method. Incubation time was carried out in 44, 46, 48, 50, and 52 hours. Organic acids produced by L. acidophilus bacteria was measured by HPLC. The result showed that COS 0.2% can inhibit the growth of E. coli and S. aureus 37.2 mm2 and 52 mm2 respectively. Combination of L. acidophilus and COS 0.2% gave inhibition zone larger than L. acidophilus without COS 0.2%. Incubation time within 48 hours of L. acidophilus combined with COS 0.2% produced the largest inhibition zone against E. coli and S. aureus 367.92 mm2 and 343.99 mm2 respectively. Optical density measurement resulted in higher value for combination one but concentration of organic acid produced was lower compare to L. acidophilus without COS 0.2%.Keywords: COS, L. acidophilus, Organic acid, antibacterial.
TRAINING PEMBUATAN SELAI DAN ES KRIM LABU KUNING SEBAGAI MAKANAN UNTUK KESEHATAN Miksusanti, Miksusanti
Jurnal Pengabdian Sriwijaya Vol 1, No 2 (2013)
Publisher : Jurnal Pengabdian Sriwijaya

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Abstract

Sebagai bahan pangan, labu kuning kaya akan vitamin A dan C, mineral, serta karbon hidrat. Buah ini mengadung zat yang berguna bagi kesehatan antara lain zat karotenoid yang berbentuk betakaroten. Komponen dalam labu kuning berfungsi melindung mata dari katarak juga serangan kangker, jantung, diabetes, ginjal, desentri, deman, serta mengandung penawar racun dan cacing pita. Berdasarkan kualitas ini, labu kuning sangat baik untuk dikembangkan sebagai makanan kesehatan. Pengabdian masyarakat tentang pengolahan labu kuning menjadi makanan kesehatan telah dilakukan di desa Karya Jaya pada bulan November 2013. Dalam kegiatan ini tim pengabdian mentraining warga desa dalam membuat selai labu kuning dan es krim labu kuning. Kegiatan pengabdian mendapat respon baik dari masyarakat desa Karya Jaya.
Aktivitas Antioksidan dan Sifat Kestabilan Warna Campuran Ekstrak Etil Asetat Kulit Buah Manggis (Garcinia mangostana L.) dan Kayu Secang (Caesalpinia sappan L.) Miksusanti, Miksusanti; Elfita, Elfita; Hotdelina S, Hotdelina S
Jurnal Penelitian Sains Vol 15, No 2 (2012)
Publisher : Faculty of Mathtmatics and Natural Sciences

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Abstract

Aktivitas antioksidan dari ekstrak etil asetat kulit buah Manggis (Garcinia mangostana L.) dan kayu Secang (Caesalpinia sappan L.) serta kombinasinya telah dipelajari dengan metode 1,1 difenil-2- pikrilhidrazil (DPPH). Campuran warna terbaik adalah perbandingan 1:2 (M1 : S2) dan telah dilakukan uji stabilitas ekstrak warnanya terhadap pengaruh pH, stabilitas terhadap oksidator, sinar UV, waktu dan suhu pemanasan, serta suhu selama penyimpanan dengan mengukur absorbansinya pada _max 440 nm. Hasil pengujian menunjukkan bahwa aktivitas antioksidan ekstrak etil asetat kulit buah Manggis lebih aktif dibandingkan dengan ekstrak etil asetat kayu Secang dengan IC50 14,1882 ppm. Ekstrak etil asetat kulit buah Manggis ini hampir sebanding dengan standar antioksidan asam askorbat yang memberikan IC50 15,3769 ppm. Kombinasi terbaik campuran ekstrak etil asetat kayu Secang dan kulit buah Manggis yang memberikan aktivitas antioksidan tertinggi dengan persen inhibisi sebesar 96% dan IC50 terendah 17,6791 ppm adalah perbandingan 1:1 (M1:S1). Semua perlakuan uji kestabilan menyebabkan perubahan warna. Warna campuran berubah dari kuning menjadi kuning pucat dan jingga. Persentase perubahan serapan warna campuran berturut-turut adalah: 26% untuk pengaruh oksidator, 34% untuk pengaruh sinar UV pada botol gelap, 35% untuk pengaruh penyimpanan suhu dingin, 97% untuk pengaruh sinar UV pada botol bening, dan 93% penyimpanan pada suhu kamar. Ekstrak etil asetat kulit buah Mangis dan kayu Secang mengandung warna yang berfungsi sebagai antioksidan yang potensial.
Studi Pengaruh Temperatur, Kelembaban dan Tekanan Udara Terhadap Polutan SO2 di Sepanjang Jalan Pelembang-Inderalaya Yuliasari, Nova; Miksusanti, Miksusanti; Eviyanti, Eviyanti
Jurnal Penelitian Sains Vol 10, No 3 (2007)
Publisher : Faculty of Mathtmatics and Natural Sciences

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Abstract

Analisa kadar SO2 di udara sepanjang jalan Palembang-Prabumulih menggunakan metoda kompleks pararosanilin secara spektrofotometri tampak telah dilakukan. Cuplikan udara diserap impinger yang disambungkan dengan pompa vakum, masung-masing cuplikan diserap selama satu jam. Hasil penelitian menunjukkan kadar SO2 tertinggi berada di kawasan publik sulfat yaitu 154,5 mg/M3 (pagi); 226,0 mg/M3 (siang) dan 201,1 mg/M3 (sore). Kadar SO2 terendah berada di Kertapati yaitu 63,2 mg/M3 (pagi); 88,6 mg/M3 (siang) dan 77,7 mg/M3 (sore). Kecenderungan hasil penelitian menunjukkan kadar SO2 siang hari selalu lebih tinggi dibanding pagi dan sore hari. Faktor kelembaban udara lebih mempengaruhi kadar SO2 dibanding temperatur dan tekanan udara. Kadar polutan SO2 yang terukur masih dibawah nilai ambang batas yang diizinkan sesuai KEP-03/MENKLH/II/1991 yaitu 260,0 mg/M3.  
Studi Biodegradasi Blend PVC-Minyak Nabati Epoksi Sebagai Salah Satu Upaya Mengurangi Pencemaran Lingkungan Oleh Limbah Plastik Desnelli, Desnelli; Miksusanti, Miksusanti
Jurnal Penelitian Sains Vol 13, No 2 (2010)
Publisher : Faculty of Mathtmatics and Natural Sciences

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Abstract

Polivinil klorida(PVC) merupakan salah satu bahan plastic yang yang banyak digunakan sebagai pengemas makanan, tetapi sulit terdegradasi. Pada penelitian ini dilakukan pencampuran polivinil klorida dengan minyak biji karet epoksi untuk mendapatkan plastic yang dapat terbiodegradasi. Komposisi poliblend yang dibuat adalah 35% berat minyak biji karet epoksi.Proses biodegradasi dilakukan dengan menggunakan bakteri Staphylococus aureus selama 30 hari. Pengamatan terhadap poliblend setelah proses biodegradasi yaitu terlihat adanya bintik-bintik coklat. Hasil analisa dengan SEM menunjukkan adanya kerusakan pada permukaan poliblend setelah proses biodegradasi. Hasil penentuan temperature leleh dan berat molekul rerata viskosistas polivinil klorida menurun setelah biodegradasi. 
Studi Penyerapan Procion pada Limbah Kain Tajung Menggunakan Serbuk Batang Eceng Gondok Yuliasari, Nova; Miksusanti, Miksusanti; Dian, Dian
Jurnal Penelitian Sains Vol 13, No 2 (2010)
Publisher : Faculty of Mathtmatics and Natural Sciences

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Abstract

Penelitian penyerapan zat warna kimiawi jenis procion pada limbah kain tajung menggunakan serbuk batang eceng gondok telah dilakukan. Konsentrasi procion dianalisis menggunakan spektrofotometer UV-Vis. Parameter yang diteliti adalah waktu kontak, berat serbuk eceng gondok dan pH untuk mendapatkan kondisi optimum. Kondisi optimum perlakuan 50 mL 100 mg/L procion, terjadi pada waktu kontak selama 30 menit, berat serbuk eceng gondok 25 mg dan pH 7. Uji statistik menggunakan ANOVA menunjukkan seluruh parameter menghasilkan data persentase penyerapan yang berbeda nyata. Konsentrasi procion awal pada limbah sebesar 441,02 mg/L. Limbah yang telah diperlakukan pada kondisi optimum memiliki konsentrasi procion menurun menjadi 222,57 mg/L. Persentase penyerapan procion dalam limbah pada kondisi optimum sebesar 49,53%.