Juli Melia
Laboratorium Reproduksi Fakultas Kedokteran Hewan Universitas Syiah Kuala, Banda Aceh

Published : 23 Documents
Articles

Found 23 Documents
Search

IDENTIFIKASI LEUKOSIT POLYMORPHONUCLEAR (PMN) DALAM DARAH SAPI ENDOMETRITIS YANG DITERAPI DENGAN GENTAMISIN, FLUMEQUIN, DAN ANALOG PGF2α Melia, Juli; a, Amrozi; Tumbelaka, Ligaya Ita; Fahrimal, Yudha
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i2.342

Abstract

Penelitian ini bertujuan mengetahui persentase leukosit polymorphonuclear (PMN) dalam preparat ulas darah sapi endometritis. Enam ekor sapi endometritis dibagi dalam dua kelompok perlakuan. Kelompok I (n=3) diterapi dengan 250 mg gentamisin/ekor, 250 mg flumequin/ekor, dan PGF2α sebanyak 12,5 mg/ekor secara intra uteri. Kelompok II (n=3) diterapi dengan menggunakan antibiotik dengan dosis dan cara pemberian yang sama seperti pada Kelompok I. Hasil penghitungan leukosit diferensial sebelum terapi menunjukkan persentase jumlah limfosit yang lebih tinggi dibandingkan bentuk leukosit lainnya pada Kelompok I dan II masing-masing adalah 62,50±1,17 dan 63,66±2,35, sedangkan persentase jumlah neutrofil pada Kelompok I dan II masing-masing adalah 29,33±0,94 dan 27,33±0,94. Setelah terapi, tidak ada perbedaan persentase (P>0,05) bentuk leukosit antara kedua kelompok perlakuan. Terapi kombinasi antibiotik dan PGF2α pada sapi penderita endometritis tidak menghasilkan perubahan diferensial leukosit termasuk PMN.
KEHADIRAN FOLIKEL DOMINAN PADA SAAT INISIASI SUPEROVULASI MENURUNKAN RESPONS SUPEROVULASI SAPI ACEH siregar, tongku nizwar; eldora, maikhar gita; melia, juli; panjaitan, budianto; y, yusmadi; barus, rina aulia
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i2.274

Abstract

Penelitian ini bertujuan mengetahui respons superovulasi dan pengaruh kehadiran folikel dominan pada saat inisiasi superovulasi sapi aceh yang diinduksi superovulasi dengan follicle stimulating hormone (FSH). Dalam penelitian ini digunakan 7 ekor sapi aceh betina yang telah didiagnosis sehat reproduksinya, umur 5-8 tahun, mempunyai berat 150-250 kg, dan mempunyai minimal dua siklus reguler. Seluruh sapi disuperovulasi dengan FSH dosis menurun pada hari ke-9 siklus estrus (3-3, 2-2, 1-1, dan 0,5-0,5) ml. Kehadiran atau ketiadaan folikel dominan pada saat inisiasi superovulasi diobservasi melalui penggunaan ultrasonografi (USG). Sapi yang mempunyai folikel kecil (3-8 mm) berjumlah <10 folikel dikategorikan mempunyai folikel dominan, sedangkan sapi yang mempunyai folikel kecil (3-8 mm) >10 folikel dikategorikan tidak mempunyai folikel dominan. Koleksi embrio dilakukan pada hari ke-7 setelah inseminasi secara non surgical menggunakan kateter Foley. Sapi yang disuperovulasi tanpa kehadiran folikel dominan menghasilkan korpus luteum (6,7+0,58 vs 4,5+1,73),folikel anovulasi (9,7+8,0 vs 19,5+6,8), total embrio (11,0 vs 3,0), dan embrio kualitas baik (6,0 vs 2,0) dibandingkan dengan sapi yang disuperovulasi dengan kehadiran folikel dominan. Dapat disimpulkan bahwa induksi superovulasi dengan FSH dengan kehadiran folikel dominan pada saat inisiasi superovulasi menurunkan respons superovulasi sapi aceh.
The Influence of Time Equilibration to The Quality of The Swamp Buffalo (Bubalus bubalis) Cement After Thawing Febriani, Gian Dina; Hamdan, Hamdan; Melia, Juli
Jurnal Medika Veterinaria Vol 8, No 1 (2014): J. Med. Vet.
Publisher : UNIVERSITAS SYIAH KUALA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v8i1.3315

Abstract

This research aims to know the influence of the equilibration to the quality of the swamp buffalo (Bubalus bubalis) cement after thawing including: motility, and percentage of live spermatozoa. This research used the fresh cement from a 4 years old water buffalo was collected using an artificial vagina,and evaluation cement for macroscopic and microskopic to see if cement was worthy or not worthy to frozen. Good quality cement was diluted by adding Andromed, then semen were incorporated into the straw using the machine filling sealing followed by equilibrated with 3 treatment groups. Group I (n = 3 straw) with equilibration time of 3 hours, the Group II (n = 3 straw) with a time of equilibration was 5 hours, and group III (n = 3 straw) with equilibration a time of 7 hours. After equilibrated, straw frozen and stored in liquid N2 (BIBD Tuah Sakato procedures). The results show that the length of time the real equilibration effect (P < 0.05) on the motility and percentage life of swamp buffalo spermatozoa. Equilibration 5 hours of time differs markedly with equilibration time 3 and 7 hours, motility, and percentage of live spermatozoa (44.15±1.70; 46.50±1.36 vs 14,55±1,58; 1.42±37.38 vs. 15.37±1.58; and 37.50±1.25). In conclusion, equilibration time is showed on the cement of swmp buffalo after 5 hours thawing.Key words: equilibration, swamp buffalo, thawing
DINAMIKA OVARIUM SAPI ENDOMETRITIS YANG DITERAPI DENGAN GENTAMICINE, FLUMEQUINE DAN ANALOG PROSTAGLANDIN F2 ALPHA (PGF2α) SECARA INTRA UTERUS Melia, Juli; A, Amrozi; ITA TumbelakaLigaya, Ligaya
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i2.2628

Abstract

Penelitian ini bertujuan mengetahui efektivitas pengobatan endometritis dengan menggunakan kombinasi antibiotik (gentamicine, flumequine) dan analog prostaglandin F2 alfa (PGF2α) berdasarkan pengamatan dinamika ovarium dengan metode ultrasonografi (USG). Enam ekor sapi endometritis dibagi dalam 2 kelompok perlakuan. Kelompok I (K1, n= 3) diterapi dengan 250 mg Gentamicine, 250 mg Flumequine, dan 12,5 mg PGF2α secara intra-uterus. Kelompok II (K2, n= 3) diterapi menggunakan antibiotik dengan dosis dan cara pemberian yang sama seperti pada Kelompok I. Hasil pengamatan terhadap sapi-sapi endometritis K1 dan K2 memperlihatkan rataan panjang siklus estrus selama 18 hari. Hasil analisis statistik menunjukkan tidak ada perbedaan yang nyata (P>0,05) terhadap kelas folikel pada kedua kelompok perlakuan. Folikel besar (DF) dan folikel besar kedua (SF) pada ke-3 gelombang folikel yang muncul tidak memperlihatkan perbedaan yang nyata antara K1 dan K2 (P>0,05), tetapi terdapat perbedaan yang nyata (P
Early Pregnancy Diagnosis Using Milk Progesterone Kits in Etawah Cross Goat (Capra hircus) Nova, Milona Elsa; Riady, Ginta; Melia, Juli
Jurnal Medika Veterinaria Vol 8, No 2 (2014): J. Med. Vet.
Publisher : UNIVERSITAS SYIAH KUALA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v8i2.3350

Abstract

This study aims to determine the effectiveness and accuracy of milk progesterone kit as an early pregnancy diagnosis tool for Etawah cross goat. This study used 9 lactating female goats with weight in the range of 35-51 kgs and the ages ranging from 2 to 2.5 years old. Eight goats were subject to estrous synchronisation protocol using progesterone implant (CIDR-G) followed by prostaglandin administration and one goat was left to show natural estrous. Early pregnancy diagnosis using milk progesterone kits was carried out on day 18 until 22 following services. The accuracy of the kits for early pregnancy diagnosis was subsequently confirmed using ultrasonography (USG) examination on day 35 following services. The results of this study showed that estrous response by synchronization was 50%. Whereas one female goat showed estrous sign naturally and was subject to pregnancy diagnosis procedure using the milk progesterone kits after natural mating. Pregnancy diagnosis using milk progesterone kits and subsequent confirmation using USG was carried out in five goats (4 synchronized females and mated and 1 naturally estrous female and mated naturally). All 5 female goats showed negative results following pregnancy diagnosis using the kits throughout the whole 5 days examinations. While diagnosis of pregnancy as confirmed by USG showed one goat (number 6) positive. It can be concluded that milk progesterone kits on dairy cows are less effective for diagnosing pregnancy on Etawah cross goat. The accuracy of the kits for pregnancy diagnosis of not-pregnant status is 80% after confirmation using USG.Key words: CIDR-G, Ettawah goats, milk progesterone kits, ultrasonography
ACEH CATTLE FOLLICLE DYNAMIC UNDER ENVIRONMENTAL HEAT STRESS Armansyah, Teuku; Siregar, Tongku Nizwan; Aliza, Dwinna; Melia, Juli; Meutia, Nellita; Panjaitan, Budianto; Hafizuddin, Hafizuddin; Adam, Mulyadi; Abrar, Mahdi
Jurnal Kedokteran Hewan Vol 11, No 3 (2017): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i3.6377

Abstract

The objective of this research is to know Aceh cattle follicle dynamic under environmental heat stress condition. This research was conducted on December 2012 until August 2013. Animals used in this research were 20 Aceh cattle aged 5-8 years old, weighing of 150-250 kg, and had at least 2 regular cycles. All cattle used were clinically in good body condition score. The cattle were divided into two groups of 10, used in two different period of times (December-January and July-August) to examine the effect of environmental heat to their follicle dynamic. Each group is separated into two different keeping management; one group was kept in pens while the others in the pasture, 5 cattle in each management group. Research timing was based on information acquired from Indrapuri Agency for Meteorology, Climatology, and Geophysics (BMKG) which predicted that extreme weather would last from July to August while December to January would be relatively normal in Aceh region. All cattle’s estrous cycle were synchronized by 5 mg/mL of PGF2α intramuscularly (Lutalyse™, Pharmacia & Upjohn Company, Pfizer Inc.). Ultrasonography examination was performed to monitor ovary follicle’s growth and dynamic during one cycle. Days during ovulation marked by standing heat was regarded as Day 0 of estrous cycle. Follicular dynamic examination during estrous cycle on December-January and also on July-August, both penned or pastured cattle showed the follicle waves that was 3 follicle growth waves. The size of follicle growth on first wave (1st DF) on cattle kept in pen on July-August and December-January were relatively similar. The size of follicle growth on first wave for cattle kept in pasture on July-August and December-January were relatively similar. To conclude, Aceh cattle’s follicle dynamic is not change during environment heat stress condition and is not affected by different cattle-keeping management.
ANATOMI DAN GAMBARAN ULTRASOUND ORGAN REPRODUKSI SELAMA SIKLUS ESTRUS PADA KUDA GAYO BETINA (Anatomy and Ultrasound Imaging of Reproductive Organs of Gayo Mares During Estrous Cycle) Melia, Juli; Agil, Muhammad; Supriatna, Iman; Amrozi, Amrozi
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): J. Ked. Hewan
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5026

Abstract

The present study examines anatomy of Gayo mare reproductive organs. This study used three sample of Gayo mare reproductive organs (n= 3) for observation of morphology and morphometric of the mare reproductive organs. The ovarium was fixed in 4% paraformaldehyde solution then followed by histological method and stained using hematoxylin and eosin (HE) and Masson’s trichome (MT). Three mares were observed for diameter and changes overview of uterus during estrous cycle in real time using ultrasound. The results showed that, in general, the anatomy of Gayo mare’s reproductive organs was similar to other mares, but smaller in morphometry. The total length of the Gayo mare’s reproductive tract from labia to apex cornua was 48.00±1.00 cm. Weight of Gayo mare’s left ovary was 19.07±7.70 g and the right was 24.43±0.83 g. Histologically, there was no difference between Gayo mare’s structure and other mares. In cortex uteri there were some follicles surrounded by capillary, various development stages of follicles, healthy follicles, atretic follicle, and corpus albican; while in medulla there were a lot of connective tissues. Ultrasound of the uterus showed the change in diameter during estrous cycle with the largest diameter of corpus uteri was 4.43±0.10 cm in horses with estrous cycle of 21 days and 6.30±0.93 cm in horses with 24 days estrous cycle. In conclusion, the morphometry of Gayo mare reproductive organs are smaller than the other horses and there are differences in diameter of the uterus during the estrous cycle due to the changes of endometrium thickness.
PENGARUH PENAMBAHAN EKSTRAK KULIT BUAH NAGA MERAH (Hylocereus polyrhizus) DALAM MEDIA SITRAT KUNING TELUR TERHADAP DAYA TAHAN HIDUP SPERMATOZOA SAPI ACEH YANG DISIMPAN PADA SUHU 4℃ (The Effect of Adding Peels Red Dragon Fruit Extract (Hylocereus polyrhizus) into Citrate-Yolk Medium to the Survival of the Aceh Cattle Spermatozoa Stored at Temperature 4℃) Wahyuni, Sonya Tri; dasrul, dasrul; hamdan, hamdan; melia, juli; rinidar, rinidar; siregar, tongku N
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 2 (2018): FEBRUARI - APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v2i2.6888

Abstract

Penelitian ini bertujuan mengetahui pengaruh penambahan ekstrak kulit buah naga merah dalam media sitrat kuning telur terhadap daya tahan hidup spermatozoa sapi aceh setelah pendinginan. Semen ditampung dari 1 ekor pejantan umur 3 tahun sehat, menggunakan vagina buatan. Semen yang berkualitas baik dibagi ke dalam 5 kelompok perlakuan penambahan ekstrak kulit buah naga merah; 0,00 g/100 ml (P0); 0,2 g/100 ml (P1); 0,4 g/100 ml (P2); 0,6 g/100 ml (P3); dan 0,8 g/100 ml (P4) dalam media sitrat kuning telur dan disimpan dalam suhu 4. Pengamatan daya tahan hidup spermatozoa dilakukan setiap hari selama 5 hari. Data yang diperoleh dianalisa dengan analysis of varian(ANOVA) pola satu arah yang dilanjutkan dengan uji berganda Duncan. Hasil pengamatan daya tahan hidup spermatozoa setelah pendinginan pada perlakuan P0; P1; P2; P3; dan P4 secara berturut-turut 3,8; 4,6; 3,6; 1,0; dan 0,0 hari. Hasil penelitian ini menunjukkan bahwa penambahan ekstrak kulit buah naga merah dalam media sitrat kuning telur berpengaruh secara nyata (P<0,05) terhadap daya tahan hidup spermatozoa. Penambahan ekstrak kulit buah naga merah 0,2 g/100 ml dalam media sitrat kuning telur menghasilkan daya tahan hidup spermatozoa sapi aceh lebih baik selama penyimpanan pada suhu 4. (This study aims to determine the effect of peels red dragon fruit extract addition into citrate-yolk medium on viability of aceh cattle sperm after cooling. Semen was collected from 1 healthy male aged 3 years, using artificial vagina. Good quality semen was divided into 5 groups; addition of peels red dragon fruit extract 0.0 g/100 ml (P0); 0.2 g/100 ml (P1); 0.4 g/100 ml (P2); 0.6 g/100 ml (P3); and 0.8 g/100 ml (P4) into citrate-yolk medium, and stored in a temperature of 4. Observation of sperm viability were done every day up to day 5. The data obtained was analyzed with the one-way analysis of variance (ANOVA), followed by multiple test Duncan. Results show sperm viability after cooling in the groups P0; P1; P2; P3; and P4 respectively was 3.8 days, 3.6 days, 4.6 days, 1.0 day, and 0.0 day. The results show that the addition of peels red dragon fruit extract in citrate-yolk medium affects significantly (P<0.05) sperm viability. The addition 0.2 g/100 ml peels red dragon fruit extract into citrate-yolk medium during storage in a temperature of 4 results greater sperm viability.)
PENGARUH PEMBERIAN GLISEROL DALAM MEDIUM TRIS KUNING TELUR TERHADAP KUALITAS SPERMATOZOA SAPI ACEH SETELAH PEMBEKUAN (The Effect of Giving of Glycerol into Tris-Yolk Medium to the Aceh Cattle Spermatozoa after Freezing) rizki, silvia; dasrul, dasrul; hamdan, hamdan; melia, juli; riady, ginta; adam, mulyadi
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 2 (2018): FEBRUARI - APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v2i2.7063

Abstract

Spermatoza yang melalui proses pembekuan seringkali mengalami kerusakan karena terbentuknya kristal es. Pembentukan kristal es dapat menyebabkan penurunan kualitas spermatozoa pasca thawing terutama penurunan motilitas. Penambahan krioprotektan dalam pengencer dapat meminimalisir kerusakan sel. Salah satu jenis krioprotektan yang sering digunakan pada pembekuan semen adalah gliserol. Penelitian ini bertujuan untuk melihat pengaruh penambahan gliserol dalam pengencer Tris Kuning Telur (TKT) terhadap kualitas spermatozoa (motilitas, spermatozoa hidup, dan MPU) sapi aceh  yang disimpan dalam nitrogen cair. Desain penelitian menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 3 perlakuan dan 6 pengulangan. Pengamatan kualitas spermatozoa dilakukan setelah pembekuan di dalam nitrogen cair. Data dianalisis dengan uji Analysis of Variance (ANOVA) pola satu arah. Hasil kualitas spermatozoa pada masing-masing konsentrasi gliserol (3%, 5%, dan 7%) berturut-turut untuk hasil motilitas sebesar: 17,17±12,17%, 51,17±11,13%, dan  33,33±8,76%; hasil spermatozoa hidup sebesar: 22,50±15,41%, 56,33±8,98%, dan 42,50±6,35%; hasil MPU sebesar: 31,42±17,90%, 61,50±10,82%, dan 42,50±12,14%. Berdasarkan hasil penelitian dapat disimpulkan bahwa penambahan gliserol dalam pengencer Tris Kuning Telur dapat mempertahankan kualitas spermatozoa (motilitas, spermatozoa hidup, dan MPU) sapi aceh setelah pembekuan. Penambahan gliserol dengan konsentrasi 5% dalam pengencer Tris Kuning Telur paling baik dalam mempertahankan kualitas spermatozoa sapi aceh dibandingkan dengan konsentrasi 3% dan 7% (Freezing spermatozoa are often experiencing the damage by the formation of ice crystal. The formation of ice crystal leads to the quality reduction of post-thawing spermatozoa especially reducing motility. The addition of cryoprotectant in the diluent can minimize cell damage. One of the most common cryoprotectant type that was used in sement freezing is glycerol. This research aims to examine the influence of the addition of glycerol in tris-yolk, or known as Tris Egg Yolk (TKT) to the quality of spermatozoa (motility, live spermatozoa, and MPU) of aceh cattles stored in liquid nitrogen. The research design used in this research is a completely randomized design, that consists of three treatments and six repetitions. Quality of spermatozoa observation were performed after freezing process in the liquid nitrogen. Data were analyzed by Analysis of Variance (ANOVA) with one tail pattern. The result of spermatozoa quality in each concentration of glycerol (3%, 5%, and 7%) respectively for the motitity as follows: 17.17±12.17%; 51.17±11.13%; and  33.33±8.76%; result of live spermatozoa as follows: 22.50±15.41%; 56.33±8.98%; and 42.50±6.35%; result of MPU as follows: 31.42±17.90%; 61.50±10.82%; and 42.50±12.14%. Based on the result, the conclusion is that the addition of glycerol in the tris-yolk can maintain the quality of spermatozoa (motility, live spermatozoa, and MPU) of aceh cattle after freezing. The addition of glycerol with 5% concentration in the tris-yolk dilator is the best concentration to maintain the quality of spermatozoa aceh cattle, compared to the 3% and 7% concentration)
MOTILITAS DAN VIABILITAS SPERMATOZOA SAPI ACEH SETELAH PEMBEKUAN MENGGUNAKAN PENGENCER SITRAT KUNING TELUR DENGAN PENAMBAHAN EKSTRAK KULIT BUAH NAGA MERAH (Hylocereus polyrhizus)(Motility and Viability Spermatozoa Aceh Cattle after Freezing Using Yolk Citrate Diluent with Additional of Peels Red Dragon Fruit Extract (Hylocereus polyrhizus)) anggraini, melisa lola; dasrul, dasrul; melia, juli; lubis, triva murtina; rosmaidar, rosmaidar; hamdan, hamdan
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 2 (2018): FEBRUARI - APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v2i2.7029

Abstract

Penelitian ini bertujuan mengetahui pengaruh penambahan ekstrak kulit buah naga merah (Hylocereus polyrhizus) dalam pengencer sitrat kuning telur terhadap motilitas dan viabilitas spermatozoa sapi aceh setelah pembekuan. Penelitian ini menggunakan semen segar sapi aceh yang dikoleksi menggunakan vagina buatan dan dibagi ke dalam 5 kelompok perlakuan pengencer dengan 5 kali ulangan. Kelompok perlakuan kontrol yaitu semen diencerkan dalam pengencer sitrat kuning telur tanpa penambahan ekstrak kulit buah naga merah (P0), kelompok perlakuan semen diencerkan dalam pengencer sitrat kuning telur ditambah ekstrak kulit buah naga merah 0,2% (P1), 0,4% (P2), 0,6% (P3) dan 0,8% (P4). Data dianalisis menggunakan uji analysis of variance (ANOVA) satu arah dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa penambahan ekstrak kulit buah naga merah konsentrasi 0,0; 0,2; 0,4; 0,6 dan 0,8% dalam pengencer sitrat kuning telur menghasilkan persentase motilitas adalah 23,60 ± 4,72; 26,60 ± 5,94; 36,40 ± 4,98; 45,40 ± 4,56 dan 33,40 ± 10,13.  Persentase viabilitas spermatozoa adalah 29,10 ± 5,82; 35,34 ± 5,85; 42,52 ± 4,87; 51,76 ± 7,13 dan 48,6 ± 6,05. Penambahan ekstrak kulit buah naga merah konsentrasi 0,6% menghasilkan persentase motilitas dan viabilitas spermatozoa yang lebih baik dibandingkan konsentrasi perlakuan lainnya. Simpulan penambahan ekstrak kulit buah naga merah dalam pengencer sitrat kuning telur dapat meningkatkan persentase motilitas dan viabilitas spermatozoa sapi aceh setelah pembekuan.(This study aims to determine the effect of addition peels red dragon fruit extract (Hylocereus polyrhizus) in the yolk citrate diluent to the motility and viability of the aceh cattle spermatozoa after freezing. This study used fresh semen of aceh cattle collected using artificial vagina and divided into 5 groups of diluent treatments with 5 replications. The control group of cement was diluted in the yolk citrate dilator without the addition of red dragon fruit skin extract (P0), semen treatment group was diluted in the yolk citrate diluent plus  peels red dragon fruit extract 0.2% (P1), 0.4% (P2), 0.6% (P3) and 0.8% (P4). Data were analyzed by using analysis of variance (ANOVA) and continued by Duncan test. The results showed that the addition of peels red dragon fruit extract 0.0; 0.2; 0.4; 0.6 and 0.8%  of yolk citrate diluent yields motility percentage is 23.60 ± 4.72; 26.60 ± 5.94; 36.40 ± 4.98; 45.40 ± 4.56 and 33.40 ± 10.13. Percentage of spermatozoa viability were 29.10 ± 5.82; 35.34 ± 5.85; 42.52 ± 4.87; 51.76 ± 7.13 and 48.6 ± 6.05.The addition of peels red dragon fruit extract 0.6% yields better percentage of motility and spermatozoa viability than other treatment doses. The conclusion of addition peels red dragon fruit extract in yolk citrate diluent can increase the percentage motility and viability spermatozoa of aceh cattle after freezing)