Articles

Action Target of Curcumin and PGV-0 on Luteal Cells Steroidogenesis through the Expression of Cytochrome P450scc Purwaningsih, Endang; Soejono, Sri Kadarsih; Dasuki, Djaswadi; Meiyanto, Edy
Journal of the Indonesian Medical Association Vol. 62 No. 4 April 2012
Publisher : Journal of the Indonesian Medical Association

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Introduction: Curcumin and its analog pentagamavuon-0/PGV-0 can inhibit steroidogenesis of luteal cell culture. However, the action site of the curcumin and PGV-0 in luteal cells steroidogenesis is still unknown.Objectives: To analyze the action site of curcumin and PGV-0 in luteal cells steroidogenesis through its influences on the expression of cytochrome P450scc enzyme.Methods: PMSG-induced corpus luteums of Sprague Dawley rat were used as the subjects. Curcumin and PGV-0 with a dosage of 100 µM was given shortly after the stimulation of LH and/or PGF2a. Immunohistochemistry staining was done to assess the expression of cytochrome P450scc.Results: LH stimulation (50 ng/ml) increased the expression of cytochrome P450scc significantly (p<0.05) compared with the control group. In the other hand, PGF2a (0.56 µM) inhibited the expression of cytochrome P450scc significantly. Curcumin (100 µM) and PGV-0 (100 µM) inhibited the expression of cytochrome P450scc in the control group and groups stimulated by LH and/or PGF2a.Conclusion: Curcumin and PGV-0 inhibit the upstream transduction signal of cytochrome P450scc in the steroidogenesis of luteal cell culture. J Indon Med Assoc. 2012;62:136-41.Keywords: P450scc, steroidogenesis, transduction signal
Aktivitas Sitotoksik Ekstrak Etanolik Rimpang Temu Kunci (Boesenbergia pandurata) Terhadap Sel Kanker Serviks HeLa dan Sel Kanker Kolon WiDr Handoko, Fransiscus Feby; Maruti, Astrid Ayu; Rivanti, Erlina; Putri, Dyaningtyas Dewi Pamungkas; Meiyanto, Edy
Majalah Kesehatan Pharmamedika Vol 3 N0 1 2011
Publisher : Majalah Kesehatan Pharmamedika

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Temu kunci (Boesenbergia pandurata) rhizome showed cytotoxic effect against T47D breast cancer cell line. It contains Panduratin, a chalcone compund, that has been investigated as chemopreventive agent. The exploration of extract of temu kuci as chemopreventive agent was expected to be an alternative for cancer therapy. The aim of this research was to determine the cytotoxic activities of ethanolic extract of temu kunci against HeLa cervix cancer and WiDr colon cancer cell line. The cytotoxic activities of ethanolic extract of temu kunci were tested using MTT assay against HeLa and WiDr cells. The IC50 values were obtained using linier regression equation. The ethanolic extract of temu kunci showed cytotoxic activities on HeLa cervix cancer and WiDr colon cancer cell lines with IC50 at 87 µg/mL and 76 µg/mL, respectively. Low IC50 values (<100 µg/mL) showed that ethanolic extract of temu kunci is potential to be developed as chemoprevention agent on cervix cancer and colon cancer. However, its molecular mecahanism need to be explored.
Aktivitas Antiproliferasi Ekstrak Etanolik Herba Ciplukan (Physalis angulata L.) Terhadap Sel Hepar Tikus Betina Galur Sprague Dawley Terinduksi 7,12-Dimetilbenz[a]antrasena Fauzi, Ilham Agusta; Amalia, Fikri; Sabila, Nurma; Hermawan, Adam; Ikawati, Muthi; Meiyanto, Edy
Majalah Kesehatan Pharmamedika Vol 3 N0 1 2011
Publisher : Majalah Kesehatan Pharmamedika

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One of the natural materials as potentially efficacious chemopreventive agents are Ciplukan (Physalis angulata L.). Several previous studies reported that Physalis angulata L. herbs ethanolic extract (PEE) has cytotoxic activity and induction of apoptosis in breast cancer cells MCF-7 and HeLa cervical cancer cells. This study aims to determine the effects of  PEE as an chemopreventive agent on rat liver cells induced 7,12-dimetilbenz[a]anthracene (DMBA). This study used Sprague Dawley strain female rats aged 40-50 days were divided into 5 groups : (1) DMBA control group, mice were induced with DMBA in per oral dose of 20 mg/kg; (2) DMBA + PEE dose 750 mg/kgBW group ; (3) DMBA + PEE dose 1500 mg/kgBW group ; (4) solvent control group of CMC-Na 0,5%; (5) PEE dose 1500 mg/kgBW control group. PEE was dissolved in CMC-Na 0,5% and administered daily, starting the seventh week after administration of DMBA. At the beginning of  the tenth week of the study, rats were necropted and liver organs were isolated and stored in buffered formalin 4%. Qualitative analysis to determine the histopathology of liver cells through staining method of Hematoxyllin & Eosin (HE), while quantitative analysis to determine the level of liver cell proliferation by AgNOR staining method. The results showed in the DMBA control group that liver cell morphology changes that hiperproliferation leading to carcinogenesis. In DMBA + PEE dose of  1500 mg/kgBW group improved the situation of DMBA-induced liver cells histopathology and antiproliferation activity better than DMBA + PEE dose of 750 mg/kgBW on DMBA-induced rat liver cells. The results showed that Physalis angulata L. herbs ethanolic extract can inhibit cell proliferation in rat liver caused by DMBA administration through antiproliferation mechanism and have potential for the development as chemoprevention material on liver cancer
The Action Target of Pentagamavunon-0 on the Luteal Cell Steroidogenesis Through Phosphorylation Measurement of Extracellular Signal Regulated Kinase Purwaningsih, Endang; Soejono, Sri Kadarsih; Dasuki, Djaswadi; Meiyanto, Edy
Journal of the Indonesian Medical Association Vol. 63 No. 2 February 2013
Publisher : Journal of the Indonesian Medical Association

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Introduction: Pentagamavunon-0 (PGV-0) compound, a curcumin analog, can inhibit steroidogenesis of luteal cell culture by inhibiting the production of progesterone. The action site of PGV- 0 on steroidogenesis of luteal cells is still unknown. The objective of this study is to analyze the effect of PGV-0 on the Extracellular Signal Regulated Kinase (ERK) phosphorylation in steroidogenesis of luteal cell culture.Methods: This study used corpus luteum of rat Sprague Dawley strain that was induced withPregnant Mares Serum Gonadotropine (PMSG). Pentagamavunon-0 was given shortly after the stimulation of LH and or PGF2a with or without forskoline. The phosphorylation of ERK was assessed by IHC method.Results: The result showed that LH stimulation increased ERK phosphorylation significantly.However, PGF2a decreased ERK phosphorylation significantly. Forskoline increased ERKphosphotylation significantly but no significant effect was found with LH stimulation. PGV-0inhibited ERK by either LH or forskoline stimulation.Conclusion: Pentagamavunon-0/PGV-0 can inhibits signal transduction of steroidogenesis lutealcells by acting on the up stream to ERK. J Indon Med Assoc. 2013;63:52-7Keywords: luteal cell, ERK, immunohistochemisty, forskolin
Structure Modification of Ethyl p-methoxycinnamate Isolated from Kaempferia galanga Linn. and Citotoxicity Assay of The Products on WiDr Cells Ekowati, Juni; Rudyanto, Marcellino; Sasaki, Shigeru; Budiati, Tutuk; Sukardiman, .; Hermawan, Adam; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 1, No 1 (2010)
Publisher : Indonesian Research Gateway

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Ethyl p-methoxycinnamate, major ingredient of Kaempferia galanga rhizome, have been reported not only has analgesic – anti inflammatory activities like NSAIDs which inhibited cyclooxygenase, but also inhibit tumor cell proliferation in specimen of mouse epidermis. Therefore, it will be interesting to carry out  synthetic studies on the derivates of ethyl  p-methoxycinnamate and searching their citotoxic activity on WiDr cell. We wish to report of structure modification on carboxyl moiety of  ethyl p-methoxycinnamate  and  evaluation on their citotoxic activity  on WiDr cell. Isolation of ethyl p-methoxycinnamate from Kaempferia galanga rhizome was carried out by percolation with ethanol 96% as solvent. Hydrolysis of ethyl p-methoxycinnamate in basic condition was performed to obtain p-methoxycinnamic acid. Preparation of some thiourea derivates of ethyl  p-methoxycinnamate was carried out  by microwave irradiation. Citotoxicity assay was carried out by MTT method for 48 h.   Modification  of  carboxyl  group  of  ethyl  p-methoxycinnamate to its thiourea form could be carried out by microwave irradiation gave; (E)-3-(4-methoxyphenyl)-N-(phenylcarba- mothioyl)acrylamide (50%); (E)-3-(4-methoxyphenyl)-N-(4-methoxyphenylcarbamothi- oyl)acrylamide (26%) and (E)-3-(4-methoxyphenyl)-N-(4-methylphenylcarbamothioyl) acrylamide (54%), yield calculated for 2 step from the acid chloride. All compounds showed no citotoxic effect on WiDr cell at 48 h incubation.
SynergisticCombinationofCiplukan(Physalis angulata) HerbsEthanolicExtractandDoxorubicinonT47DBreast CancerCells Armandari, Inna; Palupi, Kartika Dyah; Farida, Sofa; Hermawan, Adam; Susidarti, Ratna Asmah; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 1, No 1 (2010)
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Doxorubicinisoneofchemotherapeuticagentwidelyusedinbreastcancertreatment,but in high dose doxorubicin gives negative side effect, including vomit, nausea, immune suppression, and cardiac toxicity. This toxicity hopefully could be reduced by combination chemotherapy using natural herbs such as ciplukan herb. This research was conducted to explorecytotoxicactivityofsingleciplukanherbsethanolicextractanditscombinationwith doxorubicinonT47Dbreastcancercells.Cytotoxicactivityofciplukanherbsethanolicextract only and its combination with doxorubicinwere tested on T47D cells using MTT assay toobtainIC50valueandcombinationindex(CI),respectively.Singleextractshowedcytotoxic activityonT47DcellswithIC50valueofwas160*g/ml.Thus,combinationtreatmentfrom ciplukanherbsethanolicextractanddoxorubicinshowedsynergisticeffect(CI<1,0).Thiseffect wasreachedatconcentrationofciplukanherbsethanolicextract-doxorubicin80μg/ml-2nM, 80 μg/ml-4 nM, and 80 μg/ml-8 nM. This research indicated that ciplukan herbs ethanolic extractispotentialtobeappliedasco-chemotherapeuticagentinbreastcancertherapy.
Leunca (Solanum nigrum L.) Herbs Ethanolic Extract Increase Cytotoxic Activity of Cisplatin on Hela Cervical Cancer Cells Istiaji, Raditya Prima; Fitria, Maya; Larasati, .; Tjondro, Fortunella; Maruti, Astrid Ayu; Setyowati, Erna Prawita; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 1, No 1 (2010)
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Cervical cancer is one of leading causes of cancer death in women in the developing countries. The use of cisplatin as chemotherapy agent in cervical cancer is known to cause side effects and also resistance for long-term uses. One of the strategies to prevent cervical cancer based on combination agents is being developed. Leunca (Solanum nigrum L.) has been revealed to inhibit growth of human cancer cells. Therefore, it can be used in combination with cisplatin to reduce those side effects and prevent the occurrence of cell resistance. Ethanolic extract of Leunca Herb (ELH) and cisplatin were tested their cytotoxic effect on HeLa cervical cancer cell by using MTT assay to determine IC50 value. The combinationss of cisplatin-ELH were tested to determine the combination index (CI value).  The IC50 of ELH and cisplatin on HeLa cells were 227 µg/mL and 17 µM. rRespectively. Tthe study of combination resulted that almost all the index combinations were <0,9 showed  the effect of synergism combination. The Ooptimum concentration of combination was  1/8 IC50 cisplatin–1/8 IC50 ELH. The results indicated that ELH had a potency to be combination agent to enhance the activity of cisplatin on HeLa cervical cancer cells. Therefore, further study on its molecular mechanism needs to be explored.
Combination of Solanum nigrum L. Herb Ethanolic Extract and Doxorubicin Performs Synergism on T47D Breast Cancer Cells Anindyajati, .; Sarmoko, .; Putri, Dyaningtyas D. P.; Hermawan, Adam; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 1, No 2 (2010)
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Leunca (Solanum nigrum L.) has been proven to possess  anticancer activity on some type of cancer cells. In vitro study of solamargine found in the herb showed cytotoxic effect against several breast cancer cell lines, such as T47D and MDA-MB-31. Hence, further study on its potential as a co-chemotherapeutic agent needs to be conducted, in order to overcome resistance problem commonly found in cancer  chemotherapy. This study aimed to examine the cytotoxic activity of leunca herb ethanolic extract (LEE) alone and its combination with doxorubicin. Single and combinational treatment of LEE and doxorubicin on T47D breast cancer cells were done, and their viability representing cytotoxicity were analyzed by using MTT assay to determine the IC50 value and combination index (CI) to evaluate the combinational effect.  Twenty four hours-treatment of LEE  alone gave cytotoxicity activity showing a dose-dependent manner with the IC50 of 47 µg/ml, while combinational treatment showed that 4 µg/ml LEE was found to be synergist with 4 nM doxorubicin on T47D cells, with the optimum CI value of 0.59. This result shows that Solanum nigrum L. is potential to be proposed as doxorubicin co-chemotherapeutic agent against breast cancer. Further study on its molecular mechanism needs to be conducted.
Ethanolic Extract of Moringa oleifera L. Increases Sensitivity of WiDr Colon Cancer Cell Line Towards 5-Fluorouracil Nur, Kholid Alfan; Putri, Herwandhani; Cahyani, Fany Mutia; Katarina, Aulia; Susidarti, Ratna Asmah; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 1, No 2 (2010)
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For more than four decades, combination chemotherapy (co-chemotherapy) has been employed as a means to increase the effectiveness of chemotherapy regiments. The aim of our research is to investigate the activity of  Moringa oleifera  L. (tanaman kelor) ethanolic extract (MEE) as a co-chemotherapy agent with 5-fluorouracil (5-FU) on WiDr colon cancer cell line. Evaluation of MEE potency as a co-chemotherapy agent with 5-FU was based on cytotoxic activity based on percent cell viability via MTT  assay, and based on apoptosis observation via the double staining method using acrydin orange – ethidium bromide (AE) as the staining reagent.Cytotoxicity evaluation of single treatment using concentrations of 5, 20, 50, 100,125, and 250 µg/ml of MEE reduced cell viability 24 hours post-treatment. 5, 50, and 250 µg/ml of MEE was chosen as the combination concentrations with 1000 µM 5-FU. MTT assay 24 hours and 48 hours post-combination treatment showed significant cell viability reduction in comparison to those of single treatments. Apoptosis observation using the double staining method shows the presence of apoptotic cells 48 hours post combination treatment. MEE is a potential co-chemotherapy agent  by increasing the sensitivity  of WiDr colon cancer cell line towards 5-FU.
Taraxacum officinale Leaves Ethanolic Extract as Immunostimulatory Agent For Reducing Side Effect of Doxorubicin in Sprague Dawley Rats Kasianningsih, Sri; Rivanti, Erlina; Pratama, Ratih Hardika; Pratama, Nanda Resa; Ikawati, Muthi; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
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Doxorubicin as chemotherapeutic agent causes immunosuppresive. The aim  for this study to determine the effect of ethanolic extract of Taraxacum oficinale (ETO) in immunity system of Sprague Dawley  rat that induced  by doxorubicin to observe the profile of immunity cells. Sprague Dawley rats were divided into five groups each groups contain five rats : control doxorubicin group, doxorubicin dose 4,67 mg/kgBW+ ETO dose 1000 mg/kgBW, doxorubicin dose 4,67 mg/kgBW+ ETO dose 500 mg/kgBW, control extract group, and without treatment. Then the number of leukocytes, lymphocytes and neutrophils were analyzed  by hematology analyzer, whereas CD8+ T lymphocytes by flowcytometry. Results showed groups of  doxorubicin combined with ETO dose 1000 mg/kgBW and 500 mg/kgBW increased the number of leukocytes, lymphocytes, neutrophils,  cytotoxic CD8 + T cells  T cells compared  to control doxorubicin group. These data presents that etanolic extract of Jombang leaves has  immunostimulatory activity and potential as co-chemotherapy agents. Molecullar mechanism underlaying it’s immune activity need to be explored in detail.
Co-Authors -, Sukardiman . Anindyajati . Larasati . Sarmoko . Sugiyanto . Sukardiman Adam Hermawan Aditya Fitriasari Afkari, Hanif Agung Endro Nugroho Agusta Fauzi, Ilham Ahmad Fudholi Ahsani, Anisa Fauzia Ainun Wulandari, Ainun Alan Anderson Bangun Alexxander, . Amalina, Nur Dina Ameilinda Monikawati Andita Pra Darma ANINDYAJATI, ANINDYAJATI Anjarsari, Etyk Yunita Annisa Novarina Aprianto, Yoce Arief Nurrochmad Arief Rahman Hakim Asmah Susidarti, Ratna Astrid Ayu Maruti Aulia Katarina Ayu Maruti, Astrid B. Sudarto Bagaswoto Poedjomartono, Bagaswoto Barinta Widaryanti Beni Lestari, Beni Broto Kardono Chandra Risdian D., Andita Pra DA'I, MUHAMMAD Da’i, Muhammad Devi Nisa Hidayati Dewi Arum Sekti, Dewi Arum Dewi Pamungkas Putri, Dyaningtyas Dewi Pratiwi Dilalah, Idlohatud Dita Brenna Septhea Djaswadi Dasuki Dwi Ana Nawangsari, Dwi Ana Dwi Nurahmanto, Dwi Dyaningtyas D. P. Putri Dyaningtyas Dewi Pamungkas P, Dyaningtyas Dewi Dyaningtyas Dewi Pamungkas Putri Dyaningtyas Dewi Putri, Dyaningtyas Dewi Ediati Sasmito Endah Puji Septisetyani, Endah Puji Endah Puspitasari Endang Purwaningsih Erlina Rivanti Erna Prawita Setyowati Ertanto, Yogi Fadliyah, Hilyatul Fany Mutia Cahyani Feby Handoko, Fransiscus Fikri Amalia Fina Aryani Goenadi, Fina Aryani Fitria Rahmi FITRIASARI, ADTYA Fiveri, Anis Fortunella Tjondro Fransiscus Feby Handoko Hairunisa, Indah Hanif, Naufa Hendra K. Maury, Hendra K. HENDRI WASITO Herwandhani Putri Husnaa, Ulfatul Ibrahim Arifin Ika Nurzijah Ika Rahmawati Sutejo Ikawati, Muthi' Ilham Agusta Fauzi Ilmawati, Gagas Pradani Nur Imono Argo Donatus, Imono Argo Indri Kusharyanti Inna Armandani, Inna Inna Armandari Istighfari Jenie, Riris Iwan Sahrial Hamid JAKA WIDADA Juni Ekowati Kadarsih Soejono, Sri Kartika Dyah Palupi Kholid Alfan Nur Kristina, Nita Lany Candra, Lany Laras Widawaty Putri Larasati Larasati Larasati, Yonika Arum Luthfia Indriyani M, Kawaichi Mae Sri Hartati Wahyuningsih, Mae Sri Hartati Marcellino Rudyanto Maria Dwi Supriyati, Maria Dwi Marissa Angelina Masashi Kawaichi, Masashi Matsuda, Eishou Maya Fitria Moordiani ., Moordiani Muhammad Da’i, Muhammad Muhammad Fithrul Mubarok, Muhammad Fithrul Muthi Ikawati Muthi’ Ikawati N., Perdana Adhi Nanda Resa Pratama Ni Putu Linda Laksmiani Niken Nur W, Niken Novi Hastuti, Novi Novitasari, Dhania Nunuk Aries Nurulita Nurhayati, Ika Putri Nurma Sabila Nurrachma, Marsya Yonna P.K.W., Diah Ayu Perdana Adhi Nugroho Prisnu Tirtanirmala Pudjono Pudjono Putri, Asri Mega Putri, Nindya Budiana Qodria, Lailatul R A Susidarti Rachmady, Rahmawaty Rachmaniar Rahmat, Rachmaniar Raditya Prima Istiaji Rahmi Khamsita Raisatun Nisa Sugiyanto Ramadani, Ratna Dwi Ratih Hardika Pratama Ratna Asmah Susidarti Retno Arianingrum Retno Murwanti Retno Murwantib Retno Sunarminingsih, Retno Rina Andriyani Riris I Jenie, Riris I Riris Istighfari Jenie Rita Riata Rohmad Yudi Utomo Rosa Adelina Rosana Anna Ashari, Rosana Anna Rosita Melannisa, Rosita Rosye H.R. Tanjung Rul Afiyah Syarif, Rul Afiyah Santoso, Ragil Anang Saputri, Dian Sari Haryanti Sari, Nur Fitra Sarmoko Sarmoko Sendy Junedi, Sendy Sendy Junedy, Sendy Septriyanto Dirgantara Shigeru Sasaki Shirly Kumala Sismindari, ., Sismindari, Sitarina Widyarini Sofa Farida Sofia Mubarika Sri Handayani Sri Kadarsih Soejono Sri Kasianningsih Sri Susilowati Sri Tasminatun, Sri Sudarsono . Sugeng Riyanto Sugiyanto . Sugiyanto Sugiyanto Supardjan A. M., Supardjan A. Supardjan A.M., Supardjan Supardjan AM, Supardjan Susi Ari Kristina Suven ., Suven SUWIJIYO PRAMONO Tutuk Budiati Umar A. Jenie Umar Anggara Jenie Yohanes Sardjono, Yohanes Yurista Gilang Yurista Gilang Ikhtiarsyah Yuyun Farida, Yuyun Zalinar Udin Ziana Walidah, Ziana