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Characterization of Ethanol Extract from Katumpangan Air Herbs (Peperomia pellucida L. Kunth Angelina, Marissa; Amelia, Puteri; Irsyad, Muchammad; Meilawati, Lia; Hanafi, Muhammad
Biopropal Industri Vol 6, No 2 (2015)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

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Abstract

Peperomia pellucida L. Kunth known as “Katumpangan Air” is a potential medicinal plants used traditionally for uric acid, rheumatic, headache, stomachache, antimicrobial. This study aims to establish  the non-specific  and specific parameters of ethanol extract from Katumpangan air (EKA). EKA was collected from three different growth places: South Tangerang (6.833%), Bogor (7.8%) and Yogyakarta (13.125%). The results show that organoleptic extract characterization from three places were same: thick, blackish green brown, bitter and have a characteristic odor. The average content of water-soluble compounds was within range 7.39-13.29%, ethanol-soluble 15.33-16.68%, flavonoid total 3.807-4.244%. The range of moisture content was 12.25-16.34%, ash total 1.21-2.78%, acid insoluble ash 0.19-1.62%, drying shrinkage 21.62-24.98%, specific gravity 1.0010-1.0034 g/mL for the non-specific parameter. Microbial contamination testing results 0.61x103-1.13x103 coloni/g, the contaminant testing mold/yeast 0.1x102-1.7x102 coloni/g, while lead metal 0.15-0.18 mg/kg, cadmium 0-0.11 mg/kg and arsenic <0.005µg/kg.
Isolation and Characterization of Compounds from the Leaves of Pterocarpus indicus Willd and Their Antioxidant Activity Hartati, Sri; Angelina, Marissa; Meilawati, Lia; Dewijanti, Indah Dwiatmi
ANNALES BOGORIENSES Vol 20, No 1 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.941 KB) | DOI: 10.14203/ab.v20i1.224

Abstract

The flavone glycoside was isolated from ethyl acetate fraction of ethanol extract of leaves Pterocarpus indicus Willd. The isolation was conducted by gravitation column chromatography and eluted successively with hexane, ethyl acetate and methanol by gradient, and purified by sephadex-LH20. The structure was elucidated base on spectroscopy data of NMR (1D and 2D), UV, LC-MS and FT-IR. Antioxidant was evaluated using 2,2-diphenyl-1-picrylhidrazyl (DPPH) radical scavenging. The isolation and identification led a stigmasterol as Compound 1 and a new flavonol-glycoside [(2R)-7-hydroxy-3-(3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yloxy)-2-(3,4,5-trihydroxy phe nyl)chroman-4-one] or ptevon-3-D- glycoside as Compound 2. Antioxidant activity of Compound 2 showed IC50 for 18.53 mmol and blank of quercetin was 7.94 mmol and Vitamin C was 40.25 mmol. These compounds and antioxidant activities are the first time reported from this plant. 
LD50 and LC50 Values of Ethanol Extracts From Herbs of Ketumpangan Air (Peperomia pellucida (L.) Kunth) DEWIJANTI, INDAH DWIATMI; ANGELINA, MARISSA; HARTATI, SRI; DEWI, BETTY ERNAWATI; MEILAWATI, LIA
JURNAL ILMU KEFARMASIAN INDONESIA Vol 12 No 2 (2014): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

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Abstract

Ketumpangan air (Peperomia pellucida (L.) Kunth), an Indonesian wild plant, is commonly found in every Indonesian region with moist shady place. People usually consume ketumpangan air as a fresh vegetables. Empirically, ketumpangan air is consumed to treat fever, stomach disease, kidney disorders, drug abscesses, boils acne, headaches, pain in arthritic and rheumatic gout, or other external treatment. In this research, we tested the acute toxicity of ethanol extract from ketumpangan air using experimental animals mice DDY strain. We also tested the Brine Shrimp Lethality Test (BSLT) using Artemia salina Leach larva shrimp. The main aim of this reseach was to determine the LD50 and LC50 values of ethanol extract from Ketumpangan air. The results showed that the ethanol extract was categorized as ‘mild toxic’. The calculation of LD50 with Reed and Munich method showed that the LD50 value for male and female group was 15.13 g/ kg and 11.87 g/kg respectively. The BSLT result showed that the LC50 values of Ketumpangan air was 81.28 ppm.
Aktivitas Anti Agregasi Platelet dan Aktivitas Antitrombosis Ekstrak Etanol Daun Cinco (Cyclea barbata Miers.) ANGELINA, MARISSA; HARTATI, SRI; DEWI, INDAH; MEILAWATI, LIA; IWO, IMACULATA
JURNAL ILMU KEFARMASIAN INDONESIA Vol 10 No 1 (2012): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

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Abstract

Dilakukan penelitian aktivitas anti agregasi platelet dan anti trombosis ekstrak etanol daun Cinco (Cyclea barbata Miers.) (Cb) secara in vivo pada mencit. Latar belakang dari penelitian ini karena telah dilaporkan sebelumnya bahwa Cinco mempunyai aktivitas anti agregasi secara in vitro. Tujuan penelitian ini untuk mengetahui efek inhibisi ekstrak etanol daun Cb terhadap agregasi platelet serta aktivitas anti trombosis, dan juga memperpanjang waktu pendarahan dan waktu koagulasi. Hasil penelitian menunjukkan bahwa ekstrak daun Cb secara signifikan dapat menghambat agregasi platelet, memperpanjang waktu pendarahan, memperpanjang waktu koagulasi, dan menghambat pembentukan trombus secara akut. Selain itu ekstrak etanol daun Cb juga memberikan perlindungan pada thrombus secara signifikan dengan efek menyeluruh yang tergantung pada dosis. Semua pengukuran diatas dianalisa kebermaknaannya dengan menggunakan program ANOVA Satu Arah. Ekstrak etanol daun Cb dengan dosis rendah (150 mg/kg) memperlihatkan perpanjangan waktu pendarahan yang signiiikan pada ekor mencit dibandingkan kontrol dengan efek tergantung pada dosis. Ekstrak etanol daun Cb secara signiiikan memperpanjang waktu koagulasi dibandingkan terhadap kontrol dengan efek tergantung pada dosis. Ekstrak etanol daun Cb pada dosis 150 mg/kg bb dan 300 mg/kg BB signiiikan menghambat agregasi dibandingkan dengan kontrol. Dapat disimpulkan bahwa ekstrak etanol daun Cb mempunyai aktivitas anti agregasi platelet dan antitrornbosis pada dosis 150 dan 300 mg/kg BB.
in vitro Antidiabetic, Antioxidant and Cytotoxic Activities of Syzygium cumini Fractions from Leaves Ethanol Extract Artanti, Nina; Maryani, Faiza; Dewi, Rizna Triana; Handayani, Sri; Dewijanti, Indah Dwiatmi; Meilawati, Lia; Filaila, Euis; Udin, Linar Zalinar
Indonesian Journal of Cancer Chemoprevention Vol 10, No 1 (2019)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev10iss1pp24-29

Abstract

Ethanol extract of Syzygium cumini (jamblang) leaves reveal its significant α-glucosidase inhibitory activity which perform the potential activity of this plant extract as antidiabetic agent. However, other bioactivities of this plant extract have been reported. The aim of current study was to evaluate the comparison of in vitro antidiabetic, antioxidant and cytotoxic activity of S. cumini fractions. The S. cumini leaves ethanol extract was fractionated by using column chromatography with n-hexane, ethyl acetate and methanol as eluents. Seven fractions were obtained from column chromatography (F1-7). The results showed that the best antidiabetic activity was found in F5 (93% α-glucosidase inhibitory activity), the best antioxidant activity was found in F4 (83% inhibition of DPPH free radical) and the best cytotoxic activity was found in F2 (69% growth inhibition of T47D breast cancer cell line). Therefore besides in vitro antidiabetic activity, fractions of S. cumini leaves ethanol extract also showed antioxidant and cytotoxic activities. Since the results showed that the most active fractions for antidiabetic, antioxidant and cytotoxicity were differed, it could be possible to isolate the different potential active compounds for each activity.Keywords : Syzygium cumini, α-glucosidase inhibitor, DPPH, cytotoxicity