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Analisis Bahaya dan Penentuan Titik Pengendalian Kritis pada Penanganan Tuna Segar Utuh di PT. Bali Ocean Anugrah Linger Indonesia Benoa-Bali

Jurnal Perikanan Kelautan Vol 3, No 4 (2012)
Publisher : Jurnal Perikanan Kelautan

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Abstract

Penelitian mengenai analisis bahaya dan penentuan titik pengendalian kritis pada penanganan tuna segar utuh telah dilakukan selama 1 bulan dari bulan Juni hingga Juli 2012 di PT. Bali Ocean Anugrah Linger Indonesia Benoa-Bali. Penelitian ini bertujuan mengevaluasi penerapan HACCP (Hazard Analysis Critical Control Point) dalam mengidentifikasi dan mencegah potensi bahaya pada penanganan tuna segar utuh di PT. B.A.L.I . Penelitian menggunakan metode survey, dengan mengikuti secara langsung seluruh alur proses penanganan tuna segar utuh mulai dari penerimaan bahan baku sampai pengangkutan. Metode analisa data yang digunakan adalah metode analisa deskriptif. Hasil penelitian menunjukkan bahwa proses penanganan tuna segar utuh di PT. B.A.L.I telah menerapkan sistem HACCP dengan baik, hal ini terlihat dari tahapan proses penanganan telah sesuai dengan GMP (Good Manufacturing Practice) dan SSOP (Sanitasion Standar Operating Procedure) . Berdasarkan hasil identifikasi, tidak ditemukan adanya titik pengendalian kritis (CCP)  pada tahapan alur proses penanganan tuna segar utuh.

Synthesis and isolation of theaflavin from fresh tea leaves as bioactive ingredient of antioxidant supplements

Jurnal Penelitian Teh dan Kina Vol 20, No 1 (2017)
Publisher : Research Institute for Tea and Cinchona

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Abstract

Theaflavin (TF) is a product of catechin oxidation that give contribution to golden yellow color, brightness, briskness of black tea and has a potential as natural antioxidant activity. TF has a higher superoxide radical capture rate than EGCG (Epigallocatechin gallate). The study was to synthesize TF components in the form of extracts that have high antioxidants. Materials used in this research is bud+3 leaves from RITC clone (GMB 7). The research were caried out used 15 liters of tea leaves pulp per treatment, fermentation using fermentor with 15 L/min, 20 L/min and 25 L/min airflow treatment, separation of TF from thearubigin (TR) used 2.5% NaHCO3, and isolation of TF from impurity compounds used silica gel column with ethyl acetate/hexane as eluent, and DPPH (1,1-diphenyl-2-picryl hydrazyl) for antioxidant activity. The result showed that TF synthesis of fresh tea leaf catechin at water-air rate of 25 liters/minute could produced TF extract and yield TF purity extract with 1.01% and 39.15% in dry weight respectively after isolation by silica gel column. Antioxidant activity by DPPH from TF extract showed the smallest value of EC50 is 12.16 mg/L at 150 mg dose and its activity increased with the addition of concentration of TF extract.