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PERIODE BUKAAN MULUT DAN LAJU SERAPAN KUNING TELUR KAITANNYA DENGAN AKTIVITAS ENZIM PENCERNAAN PADA STADIA AWAL KERAPU BEBEK HASIL PEMBENIHAN INDUK TURUNAN KE 2 Andriyanto, Wawan; Marzuqi, Muhammad
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol 4, No 2 (2012): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Jurnal Ilmu dan Teknologi Kelautan Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

Penelitian ini bertujuan untuk mengetahui waktu bukaan mulut pertama stadia larva kerapu bebek hasil pembenihan F2 sampai dengan umur 10 HSM (hari setelah menetas) karena pada fase ini merupakan fase kritis adaptasi larva dari input makanan endogen (dari yolk sac) sampai dengan kemampuan menyerap makanan dari luar (eksogen). Telur yang digunakan berasal dari pemijahan alami induk ikan kerapu generasi ke 2 (F2) yang dipelihara pada bak ukuran 150 liter. Bukaan mulut diamati di bawah mikroskop yg terintegrasi dengan komputer, data yang didapatkan diolah dengan software dan diukur bukaan mulut 90° dan 45°. Laju serapan kuning telur diamati dari larva mulai menetas sampai dengan habis. Sedangkan aktivitas enzim pencernaan (trypsin dan chymotripsin) dianalisa dengan teknik enzim assay. Hasil penelitian menunjukkan bahwa bukaan mulut larva kerapu bebek hasil pembenihan F2 terjadi pada umur 3 HSM dengan bukaan 45° sebesar 0.103 mm dan 90° sebesar 0.156 mm. Awal stadia diameter kuning telur adalah 0.1875 mm dan gelembung minyak 0.0537 mm. Pada umur 4 HSM kuning telur dan gelembung minyak telah terserap habis, sedangkan enzim trypsin dan chymotripsin telah pula terdeteksi pada awal stadia (1 HSM). Dari hasil tersebut disimpulkan bahwa larva mulai dapat memanfaatkan makanan dari luar setelah umur 4 HSM. Sampai dengan umur 10 HSM, bukaan mulut semakin lebar dan aktivitas trypsin dan chymotripsin masih terdeteksi.Kata kunci: Larva kerapu bebek, pembenihan F2, bukaan mulut, kuning telur, enzim
PERIODS OF MOUTH OPENING AND YOLK ABSORPTION RELATED TO THE ACTIVITY OF DIGESTIVE ENZYMES IN THE EARLY STADIA FROM THE SECOND GENERATION OF HUMPBACK GROUPER BROODSTOCK Andriyanto, Wawan; Marzuqi, Muhammad
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol 4, No 2 (2012): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.29244/jitkt.v4i2.7782

Abstract

The experiment was carried out to determine the exposure time of the first mouth opening on humpback grouper larval from F2 broodstock on hatchery until 10 DAH (days after hatching) because this phase is a critical phase of larval adaptation from food endogenous (from yolk sac) to the ability of absorbing nutrients from external (exogenous). The eggs used were collected from natural spawning of second generation of humpback grouper reared in the tank size of 150 litre. The mouth opening was observed at 90 and 45 degrees under computerize integrated microscope and processed by a specific software. The rate of yolk absorption was observed in larvae from the beginning to the end of breeding, while the activities of digestive enzymes (trypsin and chymotripsin) were analyzed by enzyme assay techniques. The results showed that the period of mouth opening of larvae of humpback grouper occurred at 3 DAH with mouth openings of 45° as large as 0.103 mm and of 90° as large as 0.156 mm. In the early stadia, the diameter of yolk was 0.1875 mm, while the oil globule was 0.0537 mm. At 4 days after hatching, the yolk and oil globule had been absorbed, while the enzymes trypsin and chymotripsin were detected in the early stadia. The results also showed that the larvae was started to use foods from outside after the age of 4 DAH. Up to the age of 10 days, the mouth opening increased and the activity of trypsin and chymotripsin were still detected.Keywords: progeny larvae of humpback grouper F2, mouth opening, yolk sac, enzyme
NUTRIENT DIGESTIBILITY FEED WITH DIFFERENT LEVELS OF PROTEIN AND LIPID ON CORAL ROCK GROUPER (Epinephelus corallicola) JUVENILE Marzuqi, Muhammad; Anjusary, Dewi Nasbha
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol 5, No 2 (2013): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Jurnal Ilmu dan Teknologi Kelautan Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

The aims of this study was  to determine the nutrient digestibility coefficient of diet with diertary  levels of protein and lipid on  coral rock grouper (Epinephelus corallicola) juvenile. This study used completely randomized design with factorial arranged in 3 different treatment of dietary protein levels i.e., 36%, 42%, and 48%;and 2 different treatment  of dietary lipid levels  i.e., 9% and 18%; with 3 repetition. The average initial body weight of fishes for this study was 29.19 ± 0.97 g/fish. Fishes were reared in 18 pieces of polycarbonate tank with water volume of 30 liters with stocked density of 7 fishes/tank. The tank equipped with aeration with water flow change of 20 liters/hour. Fish were fed 2 times per day on at satiation  and fish rearing for 150 days. Parameters measured were protein, lipid, carbohydrate and energy of digestibility coefficient . The results showed that the interaction of protein and lipid levels that differ in feed ratio significantly affected on protein, lipid, carbohydrate, and energy digestibility coefficients. In general, feed with a protein content of 36% and 9% lipid  produced good nutrient digestibility coefficient  and  better economic value. Keywords: protein, lipid, feed nutrient digestibility, coral rock  grouper
PERIODS OF MOUTH OPENING AND YOLK ABSORPTION RELATED TO THE ACTIVITY OF DIGESTIVE ENZYMES IN THE EARLY STADIA FROM THE SECOND GENERATION OF HUMPBACK GROUPER BROODSTOCK Andriyanto, Wawan; Marzuqi, Muhammad
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol 4, No 2 (2012): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Jurnal Ilmu dan Teknologi Kelautan Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

The experiment was carried out to determine the exposure time of the first mouth opening on humpback grouper larval from F2 broodstock on hatchery until 10 DAH (days after hatching) because this phase is a critical phase of larval adaptation from food endogenous (from yolk sac) to the ability of absorbing nutrients from external (exogenous). The eggs used were collected from natural spawning of second generation of humpback grouper reared in the tank size of 150 litre. The mouth opening was observed at 90 and 45 degrees under computerize integrated microscope and processed by a specific software. The rate of yolk absorption was observed in larvae from the beginning to the end of breeding, while the activities of digestive enzymes (trypsin and chymotripsin) were analyzed by enzyme assay techniques. The results showed that the period of mouth opening of larvae of humpback grouper occurred at 3 DAH with mouth openings of 45° as large as 0.103 mm and of 90° as large as 0.156 mm. In the early stadia, the diameter of yolk was 0.1875 mm, while the oil globule was 0.0537 mm. At 4 days after hatching, the yolk and oil globule had been absorbed, while the enzymes trypsin and chymotripsin were detected in the early stadia. The results also showed that the larvae was started to use foods from outside after the age of 4 DAH. Up to the age of 10 days, the mouth opening increased and the activity of trypsin and chymotripsin were still detected.Keywords: progeny larvae of humpback grouper F2, mouth opening, yolk sac, enzyme
EFFECT ON DIETARY PROTEIN AND FEEDING RATE ON GROWTH OF TIGER GROUPER (Epinephelus fuscoguttatus) JUVENILE Marzuqi, Muhammad; Astuti, Ni Wayan Widya; Suwirya, Ketut
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol 4, No 1 (2012): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Jurnal Ilmu dan Teknologi Kelautan Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB)

Abstract

The grouper fish culture was developed after its succesful seed production in hatchery well known. In grow-out culture grouper, the protein requirement and feeding rate have to know well in order to understand the effectiveness on feed utilization . The experiment was designed by factorial design with the first factor as 3 dietary protein (36%, 42%, 48%) and the second factor as 3 feeding rate ( 1,5%, 2,0%, 2,5%). Ten of juvenile tiger grouper (102, 51-102, 73 g of body weight) were stocked in 27 polycarbonate tanks with 400 l volume each. Each tank was equiped with aeration and flow-through water system. Fish was fed three time per day i.e. 08:00, 11:30 and 15:30 Central Indonesia Standard Time for 16 weeks. Every week was measured body weight, survival rate, and feed quantity. The parameter used in this experiment were weight gain, spesific growth rate, feed efficiency and survival rate. The results of the experiment showed that the combination of protein level (36%, 42%, 48%) with feed ratio (1.5%, 2.0%, 2.5%) gave response on growth, weight gain and survival rate. Combination dietary protein level of 48% and feed ratio 1.5% gave higher weight gain and survival rate with values of 104.05 ± 5.89% and 96.67 ± 5.57 %, respectively.Keywords: Dietary protein and feeding rate , growth, tiger grouper (Epinephelus fuscoguttatus)
NUTRIENT DIGESTIBILITY FEED WITH DIFFERENT LEVELS OF PROTEIN AND LIPID ON CORAL ROCK GROUPER (Epinephelus corallicola) JUVENILE Marzuqi, Muhammad; Anjusary, Dewi Nasbha
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol 5, No 2 (2013): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.575 KB) | DOI: 10.29244/jitkt.v5i2.7560

Abstract

The aims of this study was  to determine the nutrient digestibility coefficient of diet with diertary  levels of protein and lipid on  coral rock grouper (Epinephelus corallicola) juvenile. This study used completely randomized design with factorial arranged in 3 different treatment of dietary protein levels i.e., 36%, 42%, and 48%;and 2 different treatment  of dietary lipid levels  i.e., 9% and 18%; with 3 repetition. The average initial body weight of fishes for this study was 29.19 ± 0.97 g/fish. Fishes were reared in 18 pieces of polycarbonate tank with water volume of 30 liters with stocked density of 7 fishes/tank. The tank equipped with aeration with water flow change of 20 liters/hour. Fish were fed 2 times per day on at satiation  and fish rearing for 150 days. Parameters measured were protein, lipid, carbohydrate and energy of digestibility coefficient . The results showed that the interaction of protein and lipid levels that differ in feed ratio significantly affected on protein, lipid, carbohydrate, and energy digestibility coefficients. In general, feed with a protein content of 36% and 9% lipid  produced good nutrient digestibility coefficient  and  better economic value. Keywords: protein, lipid, feed nutrient digestibility, coral rock  grouper
Pengaruh Kandungan Protein Pakan terhadap Pertumbuhan dan Efisiensi Pakan Benih Ikan Kakap Merah (Lutjanus argentimaculatus) Giri, Nyoman Adiasmara; Suwirya, Ketut; Pithasari, Ayu Indriani; Marzuqi, Muhammad
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.64

Abstract

The experiment was aimed to know dietary protein requirement and the effect of dietary protein levels on juvenile red snapper (Lutjanus argentimaculatus). The experiment was conducted in 18 polycarbonate tanks, with each 30 liter volume. All tanks were equipped with aeration and flow through water system to maintain good water quality. Hatchery produced juvenile red snapper of 9.4 ± 0.1 g average body weight and total length of 7.7 ± 0.4 cm was randomly selected and 10 fish was stocked in each tank. Fish were fed with experimental diets contained different levels of protein, i.e., 32, 36, 40, 44, 48, and 52%.The experiment used completely random design (CRD) in triplicates. Results showed that dietary protein levels influenced growth, feed efficiency, feed intake, body protein content, and protein retention of juvenile red snapper (p<0.05). The highest weight gain (263.7%) was found on fish fed diet with 40% protein. Based on the growth and feed efficiency data, it was concluded that dietary protein requirement for juvenile red snapper was 40%.
PENGARUH TEPUNG AMPAS KECAP SEBAGAI SUBSTITUSI TEPUNG IKAN DALAM PAKAN TERHADAP PERTUMBUHAN DAN NILAI KECERNAAN JUVENIL IKAN KERAPU MACAN (Epinephelus fuscoguttatus) Marzuqi, Muhammad; Giri, Nyoman A.; Agustina, Eva; Suwirya, Ketut
Jurnal Perikanan Universitas Gadjah Mada Vol 10, No 2 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.8892

Abstract

The aim of the study was to know the effect of soy sauce waste as substitution of fish meal in diet on the growth and dietary nutrient digestibility of juvenile tiger grouper (Epinephelus fuscoguttatus). The experiment used completely random design (CRD) with six treatments and three triplicates. The initial body weight of juvenile tiger grouper was 4.03 g and total length of 5-7 cm reared in 18 polycarbonate tanks with each 30 liter volume. Each of tanks was equipped with aeration and flow through water system. Six formulated diet with dry pellet form containing different level of soy sauce waste as substitution of fish meal, i.e., 0%, 7%, 14%, 21%, 28% and 28% added 0.15% methionine amino acid were teste. The result showed that the utilization of soy sauce waste as substitution fish meal influenced absolute growth, specific growth rate, feed conversion ratio and protein retention (P<0.05), but not significantly different (P>0.05) on survival rate of juvenile tiger grouper. Dietary nutrient digestibility (protein) obtained 98.05- 99.69%. Dietary nutrient digestibility decreased with utilization of soy sauce waste in diet. These experiments concluded that the utilization of soy sauce waste as substitution of fish meal could be used until optimal limit 21%, while the substitution of 28% can’t used for diet formulation for tiger grouper (Epinephelus fuscoguttatus).
HUBUNGAN PERKEMBANGAN MORFOLOGI DENGAN ORGAN PENCERNAAN LARVA KERAPU BEBEK (Cromileptes altivelis) TURUNAN KE-3 (F-3) Andriyanto, Wawan; Marzuqi, Muhammad
Jurnal Perikanan Universitas Gadjah Mada Vol 14, No 1 (2012)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9052

Abstract

Pengamatan perkembangan morfologi dan perkembangan saluran pencernaan larva ikan kerapu bebek F3 hasil pembenihan induk generasi F2 telah dilakukan melalui serangkaian pemeliharaan dari umur 1 HSM (hari setelah menetas) sampai dengan 40 HSM. Sampel larva diambil setiap hari dari 1 HSM sampai dengan 10 HSM dan dilanjutkan interval 3 hari sampai umur 40 HSM. Pakan alami diberikan sesuai dengan SOP perbenihan kerapu. Sampel dianalisa secara histologi dan morfologi dengan piranti lunak yang mendukung. Fase yolk sac sampai dengan pre fl exionsecara pertumbuhan tidak signifi kan, namun perkembangan morfologi dan differensiasi saluran pencernaan berkembang sangat pesat. Pada fase ini organ pencernaan larva sudah terbagi dari mulut, esophagus, perut, usus dananus namun belum sempurna. Pada fase akhir pre fl exion (13 HSM) pertumbuhan larva menunjukkan grafi k eksponensial (tumbuh cepat). Notochord terbentuk pada fase fl exion (18-20 HSM), sementara organ pencernaan terutama perut dan usus mulai berkembang. Perkembangan morfologi stadia larva kerapu bebek turunan ke-3 (F3) dari fase yolk sac sampai dengan juvenil memerlukan waktu sekitar 28 sampai dengan 31 HSM. Proporsi duri sirip punggung pada stadia prefl exion sampai dengan postfl exion memiliki panjang yang cenderung lebih tinggi dibanding kerapu yang lain yaitu 76%. Rata rata pertumbuhan panjang larva kerapu dari stadia yolk sac sampai dengan juvenil dicapai sebesar 0.41% dengan total panjang larva pada stadia juvenil adalah sekitar 18.42 ± 3.23 mmTL. Nilai standar deviasi yang tinggi memperlihatkan variasi ukuran pada akhir stadia. Larva kerapu bebek turunan ke-3 mulai memanfaatkan makanan dari luar sekitar umur 4 HSM dan saluran pencernaan mulai berfungsi optimal pada umur 28–31 HSM.
KEBUTUHAN ASAM LEMAK -3 HUFA DALAM PAKAN UNTUK PERTUMBUHAN JUVENIL KERAPU BEBEK (Cromileptes altivelis) Marzuqi, Muhammad; Giri, Nyoman Adiasmara; Suwirya, Ketut
Jurnal Perikanan Universitas Gadjah Mada Vol 7, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9066

Abstract

ω-3 HUFA are essential fatty acids for marine fish. Deficiency of these essential fatty acids is responsible for high mortality, low growth rate, incomplete formation and disfunction of swim bladder. This experiment was conducted to know dietary ω-3 HUFA requirement for growth of humpback  grouper juveniles.  Juveniles (9.8-10.3  g) were reared in 100 l of polycarbonate  tank with density of 12 juveniles/tank for 8 weeks with flow through system. The levels of ω-3 HUFA (EPA +DHA) supplementation in the test diet  were 0.0, 0.5, 1.0, 1.5, 2.0  and 3.0%. The results showed that the levels of ω-3 HUFA affected the growth and feed efficiency of humpback grouper juveniles (P<0.05). The growth of juveniles fed diet with 0.0-3.0% ω-3 HUFA were  significantly different with the growth of juveniles fed diet with 0.0 and 0.5% ω-3 HUFA. The growth of juveniles fed diet with supplementation levels of ω-3 HUFA at 1.0,  1.5,  2.0,  and 3.0% were 182.2, 168.5, 183.4, and 189.4%, respectively. Whereas supplementation of  ω-3 HUFA at 0.0 and 0.5% resulted the growth of  114.9 and 139.6%, respectively. These results indicated that the requirement of ω-3 HUFA for growth of humpback grouper should be around 1.0% in diet.ω