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Pengaruh Ekstrak Kedelai (Glycine max) Terhadap Testis dan Epididimis Tikus Jantan (Rattus norvegicus) Strain Sprague Dawley

Majalah Kedokteran Sriwijaya Vol 46, No 1 (2014): Majalah Kedokteran Sriwijaya
Publisher : Fakultas Kedokteran Universitas Sriwijaya

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Abstract

Infertilitas merupakan masalah yang dialami pria dan wanita di seluruh dunia. Salah satu tumbuhan yang dapat menyebabkan infertilitas pada pria adalah kedelai, karena  isoflavon yang terkandung dalam kedelai bersifat estrogen like dan antiandrogenik sehingga dapat menyebabkan infertilitas pada pria. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak kedelai (Glycine max) terhadap berat testis, diameter tubulus seminiferus, tebal epitel germinal tubulus seminiferus, berat epididimis, dan tebal epitel epididimis pada tikus putih jantan (Rattus norvegicus) strain Sprague Dawley. Penelitian eksperiment in vivo ini telah dilakukan dengan menggunakan 24 sampel yang dibagi menjadi 4 kelompok yaitu 1 kelompok kontrol dan 3 kelompok perlakuan. Kelompok perlakuan masing-masing diberikan ekstrak kedelai dengan dosis 2,54 mg, 3,78 mg dan 5,04 mg secara oral, kemudian pengaruh ekstrak kedelai dinilai setelah 48 hari. Setelah itu dilakukan analisis menggunakanone way ANOVA kemudian dilanjutkan dengan uji Post hoc Bonferroni. Semua analisis menggunakan komputerisasi SPSS Versi18. Dari hasil dapat disimpulkan bahwa  ada pengaruh pemberian ekstrak kedelai (Glycine max) pada dosis 3,78 mg dan dosis 5,04 mg terhadap berat testis, diameter tublus seminiferus, tebal epitel germinal tubulus seminiferus, berat epididimis dan tebal epitel epididimis tikus putih jantan (Rattus norvegicus).

Antifungi Effect of Keghenyat Leaves Fraction (Acmella uliginosa) In Vitro Study

Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol 1 No 1 (2017): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

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Abstract

Introduction Candida is a yeast fungus classes most commonly found in the oral cavity, gastrointestinal tract, reproductive tract and skin, especially the species Candida albicans. Alternative medicines such as herbs are easily available and affordable by the community, such as keghenyat leaves (Acmella uliginosa). Aim of StudyTo determine the activity of Keghenyat leaves fraction (Acmella uliginosa) compared to Nystatin against Candida albicans in vitro. MethodsIn vitro study was held in Laboratory of Genetics and Biotechnology Department of Biology, Faculty of Science and Medical Faculty, Sriwijaya University in December 2015 and January 2016. Research stages: extraction, fractionation, sensitivity test using Nystatin and Candida albicans, fractions antifungal activity test, the determination of minimum inhibitory concentration (MIC), bioautografi test and determination of compound classes. One final stages of testing the active fraction Keghenyat leaves (Acmella uliginosa) with Nystatin. Data were analyzed using ANOVA and Post Hoc Duncan test and linear regression using SPSS 20. ResultsHexane faction of Keghenyat leaves (Acmella uliginosa) is active against the fungus Candida albicans, has a Minimum Inhibitory Concentration (MIC) of 0.625% (6250μl) against the fungus Candida albicans antifungal contain active compounds that terpenoids and flavonoids, amounting to 84.68 mg / ml equivalent to 1 mg / ml Nystatin against the fungus Candida albicans. ConclusionThere is a significance different MIC between hexane fraction of Keghenyat leaves (Acmella uliginosa) and Nystatin, Nystatin have more high activity.

The Efficacy of Temu Putih Fraction (Curcuma Zedoaria (Berg) Roscoe) Related Quality and Quantity of Spermatozoa in Male Wistar Rats

Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol 1 No 1 (2017): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

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Abstract

Background Male participation in KB is still relatively low when compared to the participationof women. Researchers have to do research to find the contraception drug. Temu putih (Curcuma Zedoaria (Berg) Roscoe) is one of traditional herb that used as antifertility.   Aim of Study Aim of this study to examine change in the amount, motility, morphology, and viability spermatozoa male rats (rattus norvegicus) due to temu putih fraction suplementation.   Methods This study was an experimental study using a completely randomized design (CRD), post test with control group design. The sample in this study was  30 male rats, 10 weeks old, weight 150-200 gram. Rats were given temu putih fraction (n hexan, etylacetate and methanol-water) at dose of 300 mg/kgBB/day for 48 days. Temu putih was extracted by ethanol and did fractionation by liquid-liquid methods. The results of this study were assayed by SPSS 18.   Results The amount, motility, morphology and viability of spermatozoa in the group of metanol fraction of water  decreased  compared with the control group (p= 0,000), motilitas of spermatozoa in the group of metanol water fraction  decreased compared with the control group.   Conclusion Temu putih fraction can reduce the amount, motility, morphology, and viability of spermatozoa in male rats.   Keywords:  Fraction, Temu Putih, Amount of spermatozoa, Motility of spermatozoa, Viability Of Spermatozoa

PENGARUH BORAKS TERHADAP MOTILITAS DAN INTEGRITAS MEMBRAN SPERMA MENCIT (MUS MUSCULUS)

Jurnal Pembelajaran Biologi: Kajian Biologi dan Pembelajarannya Vol 3, No 2 (2016)
Publisher : fkip unsri

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Abstract

  Abstract: The use of food additive such as borax to preservative in food production process could be have a negative effects on health. The abuse of borax as food additive in the pempek and bakso can be dangerous for human health at Palembang city. The borax or boric acid have toxic effect on all body cells.  The purpose of the research is to assess the effect of borax on the sperm motility and integrity of sperm membrane. Including to assess, how many are the borax doses can cause decrease the sperm motility and integrity of sperm membrane of mice. The research was carried out at laboratory Department of Medical biology, Faculty of Medicine Sriwijaya University Palembang using a Completely Randomized Design with six replications. Three treatment doses of the borax were prepared, i.e. 2 mg/10 gr bw, 4 mg/10 gr bw and 6 mg/10 gr bw for P2, P3 and P4 treatment groups of mice, respectively. Each group was consisted of six reproductive male mice and controlwith aquabidest were given each day, for 35 days. The control (P1) group was administrated by similar volume of aquabidest only. The result showed that borax could decreased very significantly (P<0,01) quality of mice sperm, especially sperm motility and integrity of sperm membran compared to control (P1) group. As  a conclusion,  the administration of borax at  doses 2 mg/10 gr bw,  4 mg/10 gr bw and 6 mg/10 gr bw on male mice by gavage in 35 days can  very significantly decrease the quality of mice sperm especialy decrease of the sperm motility and increase integrity of the sperm membrane (P<0.01) compared to the control group. It is suggested to conduct further studies such as the borax can used as one choice for the man contraception. Key words: Borax, Sperm Motility, Integrity of Sperm Membran and Mus musculus.