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Antioxidant Activity of Crude Polyphenol Extract from Microwave Roasted Cocoa Bean ., Supriyanto; ., Haryadi; Rahardjo, Budi; Marseno, Djagal Wiseso
Jurnal Teknologi Dan Industri Pangan Vol 17, No 3 (2006): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (428.155 KB) | DOI: 10.6066/440

Abstract

Microwave heating is faster than the conventional way, therefore it is interesting to apply this technology for cocoa roasting. This research aimed to analyze the effect of microwave roasting of ground cocoa nib on the antioxidative properties of the crude polyphenol extract from the product.The results indicated that microwave roasting of ground cocoa nib for 5 min, adjusted at 20% of the full power (900W) gave no significant difference in the inhibition of linoleic acid oxidation and scavenging of DPPH radical activity of the crude polyphenol extract compared to that of the conventional roasting at 140 0C for 40 min. But the product showed higher reduction of ferric ion activity and lower chelating ferro ion activity. The crude polyphenol extract inhibited linoleic acid oxidation and scavenged DPPH free radical. The inhibition was lower than that of either BHT or a-tocoferol at concentrations lower than 400 ug/ml for linoleic acid oxidation and lower than 40 ug/ml for DPPH free radical scavenging. The crude polyphenol extract reduced ferric ion, though lower than BH.T Key words : Cocoa bean, microwave, roasting, polyphenol, antioxidant activity.
Pengaruh Jenis Asam dalam Isolasi Gelatin dari Kulit Ikan Nila (Oreochromis niloticus) terhadap Karakteristik Emulsi Suryanti, Suryanti; Marseno, Djagal Wiseso; Indrati, Retno; Irianto, Hari Eko
Agritech Vol 37, No 4 (2017)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.13025

Abstract

The isolation of gelatin from nila fish (Oreochromis niloticus) skin with two types of acid (acetic acid 0,10 M and citric acid 0,05 M) was carried out on the characteristics of the emulsion to know the ability of gelatin as an emulsifier. The observation has been made for gelatin including native molecule weight with non-SDS PAGE (polyacrylamide gel electrophoresis), pH, isoelectric point, and amino acid content. The characteristics of emulsion were analysed for the emulsion properties (activity and stability), viscosity, particle size, microstructure, and zeta potential. The concentrations of gelatin used in the formation of the emulsion were 0%, 0.5%, 1.0%, 2.0%, and 3.0% with soybean oil ratio was 3:1 (v/v). The results showed that the molecular weight of native gelatin from acetic acid 0.10 M was > 260 kD, whereas from citric acids 0.05 M was 260 kD. The isoelectric point of both gelatin was at pH 8.5 whereas the pH of gelatin obtained using acetic acid 0.10 M was 5.35 and citric acid 0.05 M was 4.33. The level of lipophilic amino  acid was higher than hydrophilic amino acid with the highest amount was glycine. The emulsifying properties of gelatin obatined from both acids reached the highest value of EAI (Emulsion Activity Index) at a concentration of 0.5% which decreased with the increasing of concentration. In contrary, the value of ESI (Emulsion Stability Index) and viscosity increased with the increasing of concentration. The particle size and microstructure of gelatin emulsion with acetic acid 0.10 M were greater than those with citric acid 0.05 M at the same concentration and showed to increase with the increasing of concentration. Zeta potentials of both gelatin were positive charge at all concentrations which increased with the increasing of concentration. ABSTRAKIsolasi gelatin dari kulit ikan nila (Oreochromis niloticus) dengan dua jenis asam (asam asetat 0,10 M dan asam sitrat 0,05 M) terhadap karakteristik emulsi telah dilakukan untuk mengetahui kemampuan gelatin sebagai emulsifier. Pengamatan terhadap gelatin meliputi berat molekul native dengan non-SDS PAGE (polyacrylamide gel electrophoresis), titik isoelektrik, pH dan asam amino. Pengamatan terhadap karakteristik emulsi meliputi sifat (aktivitas dan stabilitas), viskositas, ukuran partikel, dan mikrostruktur emulsi serta zeta potensial. Konsentrasi gelatin yang digunakan dalam pembentukan emulsi yaitu 0%, 0,5%, 1,0%, 2,0%, dan 3,0% dengan menambahkan minyak kedele pada rasio 3:1 (v/v). Hasil penelitian menunjukkan berat molekul gelatin native dari asam asetat 0,10 M adalah > 260 kD sedangkan dari asam sitrat 0,05 M sebesar 260 kD. Titik isoelektrik kedua gelatin pada pH = 8,5 sedangkan pH gelatin dari asam asetat 0,10 M sebesar 5,35 dan dari asam sitrat 0,05 M sebesar 4,33. Kandungan asam amino lipofilik dari kedua gelatin lebih banyak daripada asam amino hidrofilik dengan kandungan yang terbesar adalah glisin. Sifat emulsi gelatin dari kedua proses asam tersebut memiliki nilai EAI (Emulsion Activity Index) tertinggi pada konsentrasi 0,5%, yang semakin menurun dengan semakin meningkatnya konsentrasi. Sebaliknya nilai ESI (Emulsion Stability Index) dan viskositas emulsi semakin meningkat dengan semakin meningkatnya konsentrasi. Ukuran partikel droplet dan mikrostruktur emulsi gelatin dari proses asam asetat 0,10 M lebih besar daripada proses asam sitrat 0,05 M pada konsentrasi yang sama dan menunjukkan peningkatan dengan semakin meningkatnya konsentrasi. Zeta potensial kedua gelatin bernilai positif pada semua konsentrasi dan meningkat dengan semakin besarnya konsentrasi.
Pengaruh Penyangraian dengan Enerji Gelombang Mikro Terhadap Polifenol dalam Hancuran Keping Biji Kakao Supriyanto, Supriyanto; Haryadi, Haryadi; Rahardjo, Budi; Marseno, Djagal Wiseso
Agritech Vol 26, No 3 (2006)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.9592

Abstract

Polyphenols play important roles in taste and flavour development as well as antioxidative property of cocoa products. Chemical changes of the polyphenols occurring during conventional roasting was due to exposure to the oxygen of surrounding air at high temperature(110-200oC) for 20 up to 60 min, depend on the degree of roasting. Shorter time was needed to roast the cocoa nib by using microwave energy. The objective of this research was to investigate changes of polyphenol compounds during microwave roasting of ground cocoa nibs. Deshelled and ground cocoa nib passing through 20 mesh screen was roasted using microwave oven at 20% power for 5 min. Conventional roasting conditions were also adopted for treating the sample using an electric oven at 140 oC for 40 min as comparison. The polyphenols was extracted with acetone 80% and then freez dried. The crude extract was characterized for total polyphenols. Polarity of the polyphenolic fractions was analyzed from the crude extract as well as the ethyl acetate soluble extract by HPLC. Chemical bond of the compounds were detected by Fourier Transform Infrared Spektrophotometer (FTIR). Results indicated that the conventional roasting tend to decrease the polyphenols content while microwave roasting was increase, though the data showed significantly no difference (p≤0,05) . HPLC separation suggested that the roasting of the sample  increased in polarity, though FTIR spectra showed similarity in quantity of O-H, C-H, and C=C bonds respectively The microwave oven roasting gave one small additional fraction compared to both sample extracts from the conventional roasted and the unroasted samples which gave two fractions. Further, the ethyl acetate soluble extract from the microwave sample gave eight chromatographic fractions while from those conventional roasted and the unroasted gave two fractions and one fraction, respectively.
Kinetika Pertumbuhan Mikrobia dan Kemunduran Mutu Bakso Berpelapis Edible Aktif Berbasis Pati Kimpul (Xanthosoma sagittifolium) pada Berbagai Ketebalan Warkoyo, Warkoyo; Rahardjo, Budi; Marseno, Djagal Wiseso; Karyadi, Joko Nugroho Wahyu
Agritech Vol 35, No 4 (2015)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.9330

Abstract

Quality deterioration of food material with active edible coated depends on product properties, environment andproperties of coating. Properties of coating are influenced by the type and amount of the basic constituent materials, plasticizers, and active coating ingredient. The objective of this research was to determine the kinetics of microbial growth, and quality deterioration of X. sagittifolium starch-based coated meatball with various thicknesses. The treatments tested in this study were thickness of edible coatings (0.43; 0.48; 0.53; 0.58 mm). Observation parameters were the number of microbes (total microbial, and Pseudomonas aeruginosa), protein content, TVB-N, water losses, and texture. The results showed that active edible coatings thickness significantly affected of microbes number, and TVB-N, but did not affect protein content, water losses, and meatball texture. Microbial growth (TPC and P. aeruginosa) changed exponentially with the rate of increase of 0.049 to 0.055 per hour for TPC and from 0.071 to 0.075 per hour for P. aeruginosa or 0.026 per mm.hour times the thickness. TVB-N content changed linearly with the rate of increase of 0.132 to 0.153 mg/100g.hour or 0.206 mg/100g.mm.hour times the thickness. X. sagittifolium starch-based active edible coating with potassium sorbate as active ingredient and 0.43 mm coating thickness could prolong meatball shelf life up to 4 times longer than control.ABSTRAKKemunduran mutu bahan pangan yang terlapisi pelapis edible aktif tergantung kepada sifat produk, kondisi lingkungan, dan karakter pelapis. Karakter pelapis dipengaruhi oleh jenis dan jumlah dari bahan dasar penyusun, plasticizer, dan bahan aktif yang digunakan. Tujuan penelitian ini adalah untuk menentukan kinetika pertumbuhan mikrobia, dan kemunduran mutu bakso terlapisi edible aktif berbasis pati umbi kimpul (X. sagittifolium) dengan berbagai ketebalan. Perlakuan dalam penelitian ini adalah ketebalan pelapis edible yang berbeda (0,43; 0,48; 0,53; 0,58 mm). Parameter pengamatan meliputi jumlah mikrobia (total mikrobia dan Pseudomonas aeruginosa), kadar protein, TVB-N, susut bobot, dan tekstur. Hasil penelitian menunjukkan bahwa ketebalan pelapis edible aktif berpengaruh nyata terhadap populasi mikrobia, dan TVB-N, serta berpengaruh tidak nyata terhadap kadar protein, susut bobot, dan tekstur bakso. Pertumbuhan mikrobia (TPC dan P. aeruginosa) berubah secara eksponensial dengan laju peningkatan sebesar 0,049-0,055 per jam untuk TPC, dan 0,071-0,075 per jam untuk P. aeruginosa atau sebesar 0,026 per mm.jam kali ketebalan. Kadar TVB-N bakso berubah secara linier dengan laju peningkatan sebesar 0,132-0,153 mg/100g.jam atau sebesar 0,206 mg/100g.mm.jam kali ketebalan. Pelapis edible aktif berbasis pati X. sagittifolium yang diinkorporasi kalium sorbat dengan tebal 0,43 mm dapat meningkatkan umur simpan bakso sampai 4 kali lebih lama dibandingkan bakso tanpa pelapis.
Sifat Fisik, Mekanik dan Barrier Edible Film Berbasis Pati Umbi Kimpul (Xanthosoma sagittifolium) Yang Diinkorporasi dengan Kalium Sorbat Warkoyo, Warkoyo; Rahardjo, Budi; Marseno, Djagal Wiseso; Wahyu Karyadi, Joko Nugroho
Agritech Vol 34, No 1 (2014)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.9525

Abstract

Xanthosoma sagittifolium starch has the potential to be developed as a base for edible film, because it was hydrocolloid compound, as well as renewable natural resources, widely available and easy to obtain its. High amylose content of X. sagittifolium starch (35.34%), twice than cassava starch, so that it possible to produce strong and flexible edible film. The presence of the active ingredient in edible film with different it kinds and amounts would produce different properties too. The purpose of this study was to evaluate the physical, mechanical and barrier properties of active edible film due to variation of concentration of starch and potassium sorbate. Edible films have produced characteristics for tensile strength from 0.399 to 1.390 MPa, elongation from 14.943 to 31.647%, thickness from 0.065 to 0.081 mm, water vapor transmission rate from 10.095 to 15.247 g.mm/m2.day, solubility from 27.126 to 59.846% and transparency from 0,719 to 1,063. To increase starch made elevation of edible film tensile strength, thickness, WVTR, and the smoothness, as well as decrease its water solubility, while increasing potassium sorbate would increase elongation and WVTR, as well as decrease the tensile strength. ABSTRAKPati umbi kimpul (Xanthosoma sagittifolium) mempunyai potensi untuk dikembangkan sebagai bahan dasar edible film, karena pati merupakan senyawa hidrokoloid, sebagai sumber daya alam yang dapat diperbaharui, tersedia secara luas dan mudah didapat. Kandungan amilosa pati umbi kimpul cukup tinggi (35,34%), dua kali lebih besar dibandingkan amilosa pati ubi kayu, memungkinkan untuk menghasilkan edible film yang kuat dan fleksibel. Keberadaan kalium sorbat dalam edible film dengan jumlah yang berbeda akan menghasilkan sifat yang berbeda pula. Tujuan penelitian ini adalah untuk mengetahui sifat fisik, mekanik dan barrier edible film aktif akibat variasi konsentrasi pati umbi kimpul dan kalium sorbat. Edible film yang dihasilkan mempunyai karakter kuat tarik 0,399-1,390 MPa, persen pemanjangan 14,943-31,647%, ketebalan 0,065-0,081 mm, WVTR 10,095-15,247 g.mm/m2.hari, kelarutan 27,126-59,846% dan transparansi 0,719-1,063. Penambahan pati menyebabkan kuat tarik, ketebalan, laju transmisi uap air, dan kehalusan permukaan edible film meningkat, tetapi kelarutannya dalam air menurun, sedangkan penambahan kalium sorbat dalam edible film menyebabkan persen pemanjangan dan laju transmisi uap air meningkat, tetapi kuat tariknya menurun.
A SIMPLE AND SENSITIVE METHOD FOR DETERMINATION OF SUGAR CONTENT IN FRUITS AND CULTURE MEDIA DURING FERMENTATION Marseno, Djagal Wiseso; Indrati, Retno
Jurnal Agritech Vol 29, No 04 (2009)
Publisher : Jurnal Agritech

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

A colorimetric for sugar determination using cuprammonium reagent containing strong base has been improved with a heating process after a reaction started.  The reaction was started after the sugar or sample containing sugar was added to the cuprammonium reagent consisted of 1 mM CuSO – 0,4 M (NH ) SO – 0,6 M NaOH).  This heat­ ing process could enhance the formation of sugar­cupper ammonium complex, so that the intensity of absorbance increased at wavelength of 280 nm. This simple and sensitive method was applicable for various sugars detection such as sugar fraction after HPLC separation, sugar content in biological extract or in culture medium during fermentation. Interestingly, different from previous report, this method was specific for non polyol sugars.
APLIKASI EDIBLE FILM KERAGINAN DAN Eucheuma sp. UNTUK MENGHAMBAT SUSUT BERAT BUAH ANGGUR HIJAU Manuhara, Godras Jati; Marseno, Djagal Wiseso; Santoso, Umar
Caraka Tani: Journal of Sustainable Agriculture Vol 23, No 2 (2008): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1344.78 KB) | DOI: 10.20961/carakatani.v23i2.13982

Abstract

Carrageenan extracted from Eucheuma sp. red algae has potential benefit to develop edible film. Eucheuma Sp. red algae used in this research were harvested from Pantai Selatan, a beatiful beach lies at Gunung Kidul resort, Jogjakarta Province, Indonesia. First objective of this research was proximat analysis of coarse-ground Eucheuma sp. red algae. Thirdly, weight loss analysis of green grapes wrapped by carrageenan edible film. Coarse-ground Eucheuma Sp. algae containde water, ash, protein, fat, and carbohydrate (by different) respectively 11,20%, 18,19%, 15,42%, 2,19% and 53,0%. Carrageenan extracted from Eucheuma sp. contained lower sulphuric (13,79%) than commercial carrageenan (Wako Chem.). Carrageenan edible film wrapping was not effective to reduce weight loss of green grapes.
Pengaruh Metode Kombinasi Autoklaf 2 Siklus dan Hidrolisis Asam Sitrat terhadap Sifat Kimia dan Fisika RS-3 Pati Kacang Hijau (Vigna radiata L.) Triwitono, Priyanto; Marsono, Yustinus; Murdiati, Agnes; Marseno, Djagal Wiseso
Agritech Vol 37, No 3 (2017)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.11620

Abstract

Mung beans are potential sources of amylose as ingredients low-calorie RS-3. Studies on physical and chemical properties are most important for the obesity treatment in the future. Preparation method of RS-3 using a combination treatment of 2 cycles autoclaving and citric acid hydrolysis on mung bean starch of Walet varieties and the effect on chemical and physical properties were conducted. The result showed that the preparation method improved amylose content by 20% and RS by 47.3%, decrease the swelling power by 47.6%, increase in the WHC by 237.8% and OHC by 9.3%. The starch color also become brighter with the ∆ E 32.6. Its viscosity became lower with a setback of 710 Cp. The amilograph curve type changed from type C to D. The granule shape become irregular and the size increase three-fold. The diffraction pattern has steady in type C but the intensity increased.                                                              ABSTRAKKacang hijau merupakan jenis kacang-kacangan sumber amilosa yang potensial sebagai bahan dasar RS-3 rendah kalori. Kajian tentang sifat fisika dan kimiawinya membuka peluang pemanfaatannya untuk penanganan obesitas di masa datang. Telah dilakukan preparasi RS dari pati kacang hijau varietas Walet dengan perlakuan kombinasi autoklaf 2 siklus dan hidrolisis asam sitrat dan dikaji pengaruhnya terhadap sifat kimia dan fisikawinya. Hasil analisis menunjukkan bahwa perlakuan kombinasi autoklaf 2 siklus dan hidrolisis asam sitrat mampu meningkatkan kadar amilosa sebesar 20% dan RS sebesar 47,3%, menurunkan swelling power sebesar 47,6%, meningkatkan WHC sebesar 237,8% dan OHC sebesar 9,3%. Pengaruh lainnya  yaitu mampu menurunkan derajat putih warna pati dengan ∆ E sebesar 32,6, menurunkan viskositas dengan viskositas balik 710 Cp. Tipe kurva amilografi berubah dari tipe C ke tipe D, bentuk granula tidak beraturan, ukuran granula meningkat tiga kali lipat, dan pola difraksinya tidak berubah (tetap tipe C) tetapi intensitasnya meningkat.Kata kunci: Amilosa; autoklaf; obesitas; asam sitrat; kacang hijau; RS; pati 
Penyangraian Hancuran Nib Kakao dengan Enerji Gelombang Mikro untuk Menghasilkan Cokelat Bubuk Supriyanto, Supriyanto; Marseno, Djagal Wiseso
Agritech Vol 30, No 4 (2010)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.9715

Abstract

In this experiment, energy from a microwave oven adjusted at 20 % scale out of 900 watt for 5 min was applied to roast ground cocoa nib passing through 20 mesh screen for 5 min to produce cocoa powder. Effect of the technique on the physical and chemical properties of the product was compared to that of the conventional roasting at 140oC for 40 min. The result indicated that cocoa powder which was produced by microwave roasting had lower water content (3.48% compared to 3.88%); lower fat content (23.56% compared to 25.18%). It had smaller particle size and more uniform (10-45 µm compared to 20 – 125 µm), however both of them had the same brown intensity. Flavor intensity, color and acceptability of cocoa powder produced by microwave oven did not show a significant difference (p<0.05), however it had more bitter taste, and comprised more flavor components compared to that of resulted by conventional roasting. Polyphenol content and antioxidant activity of cocoa powder produced by microwave and conventional roasting were not significant difference (p<0.05).ABSTRAKDalam penelitian ini dilakukan penyangraian hancuran keping biji kakao (nib) lolos ayakan 20 mesh, mengguna- kan enerji gelombang mikro (EGM), untuk pengolahan bubuk cokelat. Pengaruh cara penyangraian tersebut dikaji terhadap sifat fisik dan kimia bubuk cokelat yang dihasilkan, dibandingkan dengan penyangraian konvensional. Pe- nyangraian nib kakao menggunakan EGM dilakukan selama 5 menit diatur pada posisi power 20 % dari 900 watt, penyangraian konvensional dilakukan pada suhu 140 oC selama 40 menit. Bubuk cokelat hasil penyangraian meng- gunakan EGM mempunyai kadar air lebih rendah (3,48 % dibanding 3,88 %), kadar lemak lebih rendah (23,56 % dibanding 25,18 %), ukuran partikel hancuran lebih kecil (10 – 45 µm dibanding 20 – 125 µm) dan lebih merata, serta intensitas warna cokelat tidak berbeda dibanding bubuk cokelat hasil penyangraian konvensional (p<0,05). Bubuk cokelat dari hasil penyangraian dengan EGM mempunyai kekuatan flavor, warna, dan kesukaan tidak berbeda, tetapi berasa lebih pahit dan jumlah komponen flavor yang terbentuk lebih banyak. Kandungan polifenol dan aktivitas anti- oksidan bubuk cokelat hasil penyangraian dengan EGM tidak berbeda dengan bubuk cokelat dari hasil penyangraian konvensional(p<0,05)
Kinetika Pertumbuhan Mikrobia dan Kemunduran Mutu Bakso Daging Terlapisi Pati Umbi Kimpul (Xanthosoma sagittifolium) yang Diinkorporasi Kalium Sorbat Warkoyo, Warkoyo; Rahardjo, Budi; Marseno, Djagal Wiseso; Wahyu Karyadi, Joko Nugroho
Agritech Vol 35, No 1 (2015)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.9420

Abstract

The rapidity of microbial growth as damage agents indicates quality deterioration of food as its substrate. The presence of active coatings on the surface of the food material will affect to both of microbial growth rapidity and food quality deterioration. The objectives of this research were to determine the kinetics of microbial growth and quality deterioration of X. sagittifolium starch-based coated meatballs incorporated with potassium sorbate. Various potassium sorbate concentration onedible coatings were tested in the research. Observation parameters included the number of microbes, protein content, TVB-N, weight losses, and texture. The results showed that the addition of potassium sorbate significantly affected the number of microbes, TVB-N, and texture. However, it did not significantly affect to the protein content and weight losses of meatballs. The number of microbes increased exponentially with the inhibition rate of 0.101 times of potassium sorbate concentration, while TVB-N and the texture of meatballs changed linearly with the inhibition rate of 0.584 and 0.036 times of potassium sorbate concentration respectively. The active starch-based edible coatings with 0.6% potassium sorbate incorporated increased the shelf life of meatballs up to 4 days, while the meatballs without coating only lasted less than 1 day.ABSTRAKKecepatan pertumbuhan mikrobia sebagai agen kerusakan dapat mengindikasikan kemunduran mutupangan sebagai substratnya. Adanya pelapis aktif pada permukaan bahan makanan akan mempengaruhi keduanya.Tujuan penelitian ini adalah untuk menentukan kinetika pertumbuhan mikrobia, dan kemunduran mutu bakso terlapisi pati umbi kimpul (X. sagittifolium) yang diinkorporasi kalium sorbat. Perlakuan yang dicoba dalam penelitian ini adalah konsentrasi kalium sorbat yang berbeda pada pelapis edible. Parameter pengamatan meliputi jumlah mikrobia, kadar protein, TVB-N, susut berat, dan tekstur.Hasil penelitian menunjukkan bahwa penambahan kalium sorbat berpengaruh nyata terhadap jumlah mikrobia, TVB-N, dan tekstur, serta berpengaruh tidak nyata terhadap kadar protein dan susut berat bakso. Jumlah mikrobia meningkat secara eksponensial dengan laju penghambatan 0,101 kali konsentrasi kalium sorbat, sementara  TVB-N, dan tekstur bakso berubah secara linier dengan laju penghambatan masing-masing sebesar 0,584 dan 0,036 kali konsentrasi kalium sorbat. Pelapis edible aktif berbasis pati X. sagittifolium yang diinkorporasi kalium sorbat 0,6% dapat meningkatkan umur simpan bakso sampai 4 hari, sementara bakso tanpa pelapis hanya bertahan kurang dari 1 hari.