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TOKSISITAS DETERJEN TERHADAP BENIH IKAN KAKAP PUTIH (Lates calcarifer, Bloch) Nedi, Syahril; Marnis, Huria
Jurnal Terubuk Vol 33, No 2 (2006)
Publisher : Jurnal Terubuk

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Abstract

The research of the toxicity of detergent on Barramundi (Lates calcalifer, Bloch) has been done in atLaboratory Loka Budidaya Laut Batam on Maret 2005. The aim of this research was to test the toxicity ofdetergent on Barramundi based on the result from LC50 and to take the point of safety concentration onBarramundi life. The pre test was get the result from upper treshold (N) = 8 3 ppm and lower treshold (n) = 81ppm. Persistant test showed the result that media test must be changed abaut 6 hours at toxicity test. Theresult of LC50 24, 48, 72 and 96 hours about 121,35 ppm; 100, 35 ppm; 91,95 ppm; 84,35 ppm. The safetyconcentration from detergent was about 8,435 ppm.
DETEKSI DAN ANALISIS EKSPRESI TRANSGEN (PhGH) PADA IKAN LELE DUMBO (Clarias gariepinus) TRANSGENIK F3 (Detection and Analysis Expression of transgene (PhGH) in Dumbo Catfish (Clarias gariepinus) Transgenic F3) Sihotang, Fery Jaksen; Utomo, Budi; Lesmana, Indra; Marnis, Huria
AQUACOASTMARINE Vol 9, No 4 (2015): AQUACOASTMARINE
Publisher : Program Studi Manajemen Sumberdaya Perairan, Fakultas Pertanian Universitas Sumatera Utara

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Abstract

Fisheries output need tobe increased to meet the rising demands for fish incomingyears as the increasing of human population. Biotechnology approach has used forincreasing production on aquaculture sector. One of the advances biotechnologies thatused for aquaculture development is transgens technology. Transgenic fish is a speciesthat experience alteration of genetic structure that introduced from other organisms,with the result that change the their genetic function in accordance with the introducedgene. One of the things that support the development of transgenic fish strain formationis the ability of individual transgenic to bequeath the transgenes to the next generation,so that the genetic trait target genes stable in the resulting offspring. This study aims todetect and determine the expression of growth hormone gene (PhGH) on dumbo catfish(Clarias gariepinus) transgenic F3. Transgene detection performed on embryos andlarvae of catfish transgenic F3 using PCR with primers ACT 107 and PhGH2.Transgene expression was analyzed by using reverse transcriptase polymerase chainreaction (RT-PCR) methods with primers β-actin and ACT 107 and PhGH2. Theresults showed that the transgene was detected in some of the embryos and larvaeaccording to Mendel principle. β-actin primer amplification compared to primary ACT107 and PhGH2 showed that transgene can be expressed in some of the embryos andlarvae.Keywords: biotechnology, aquaculture, transgenic, detection, expression, growthhormone
PENGUJIAN PERILAKU PREDATOR DAN ANTI-PREDATOR PADA IKAN LELE (Clarias gariepinus Burchell, 1822) TRANSGENIK F3 Testing Predator and Anti-Predator Behavior in African Catfish (Clarias gariepinus Burchell, 1822) Transgenic F3 Widyawati, Putri; Bakti, Darma; Desrita, Desrita; Marnis, Huria
AQUACOASTMARINE Vol 9, No 4 (2015): AQUACOASTMARINE
Publisher : Program Studi Manajemen Sumberdaya Perairan, Fakultas Pertanian Universitas Sumatera Utara

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Abstract

African catfish (Clarias gariepinus Burchell, 1822) transgenic containing Pangasius hypothalmus Growth Hormone (PhGH) has better performance compared with non-transgenic. However, before transgenic African catfish are released and utilized, their genetic impact on the natural environment must be examined. Predator avoidance is one of the major fitness traits determining potential environment risk. Transgene detection was performed using the PCR (Polymerase Chain Reaction) method and electrophoresis. Furthermore, the observation of the performance of growth including the growth of the length (the average length of the fish at the end - the average length of the fish at the beginning), the growth in weight (average weight of fish in the end – average fish at the beginning) and the survival rate (the number of fish that live in end / number of fish lived at the beginning). Results indicate that non-trangenic had better predator avoidance than transgenic African catfish. Non-transgenic has a better growth performance than transgenic African catfish and has a higher survival rate. Transgenic African catfish could be used for commercial aquaculture without affecting the natural environment because the increased susceptibility of transgenic to predators would most likely decrease or eliminate the transgenic genotype.Keywords: Clarias gariepinus, Transgenic, Environmental Risk
TRANSMISI TRANSGEN (PhGH) DAN PERFORMA PERTUMBUHAN IKAN LELE (Clarias gariepinus) TRANSGENIK F-3 Transmission of Transgene (PhGH) and Growth Performance Catfish (Clarias gariepinus) Transgenic F-3 Habibullah, Sultan Akbar; Nasution, Zulkifli; Djayus, Yunasfi; Marnis, Huria
AQUACOASTMARINE Vol 9, No 4 (2015): AQUACOASTMARINE
Publisher : Program Studi Manajemen Sumberdaya Perairan, Fakultas Pertanian Universitas Sumatera Utara

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Abstract

Fish increasing in aquaculture production requires the fulfillment of the need toaccelerate the productivity. Transgenesis technology with the introduction of transgenes(PhGH) can be performed to produce the fish that grow faster and has better performance.Transgene detection was performed using the PCR (Polymerase Chain Reaction) method andelectrophoresis using seed of transgenic catfish F-3. Furthermore, the observation of theperformance of catfish transgenic F-3 by measuring the rate of fertilization (the number offertilized eggs / total eggs), hatchability rate (the number of hatched eggs / number offertilized eggs), the survival rate (the number of early fish / amount of fish end), theperformance of growth (weight average transgenic F-3 / weight average non-transgenic) andfeed efficiency (absolute growth rate / daily feed intake). Results indicate that thetransmission rate ranged from 5-75% with an average transmission in the female transgenikF-2 by 2.5% (15/600) and 14.4% (75/520) in the male transgenik F-2. Weight growth rate of1.7 fold compared with non-transgenic. F-3 transgenic catfish has a degree of fertilization0.37% smaller and has a hatching 4% higher than the non-transgenic. Transgenic catfish feedefficiency higher than non-transgenic This shows that transgenic catfish has betterperformance compared with non-transgenic.Keywords: Transgenic catfish F-3, transgene (PhGH), transmission, Polymerase ChainReaction, growth performance
MORPHOLOGICAL CHARACTERISTICS OF A RED STRAIN OF THE EGYPTIAN AFRICAN CATFISH (Clarias gariepinus BURCHELL 1822) Iswanto, Bambang; Suprapto, Rommy; Marnis, Huria; Imron, Imron
Indonesian Aquaculture Journal Vol 11, No 2 (2016): (December, 2016)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (495.823 KB) | DOI: 10.15578/iaj.11.2.2016.%p

Abstract

Characteristics of the Egyptian African catfish (Clarias gariepinus) strain introduced to Indonesia has not been extensively explored yet, especially the red strain. Previous studies suggested that at the same body length, body weight of the red strain was higher and it was more rotund than that of the normal (black) ones. These differences need to be further investigated to find out which parts of the body mainly contributed to shape the differences. The present study was carried out to explore morphological differences of the red strain of Egyptian African catfish compared to the black strain through morphometric and meristic characterizations. Meristic and morphometric characterizations in the present study were carried out following standard method for morphological characterization of Clarias catfish. The fish samples consisted of each 35 red and black table-sized fish samples resulted from inbred and outbred spawnings. Results of the morphometric and meristic analysis in the present study revealed that the red strain of Egyptian African catfish resulted from inbred spawning of red strain brooders was morphologically different from that of either parental fishes or the black strains. At the same body length, head of the red strain was bigger (wider and longer) than other strains, and its body was stumpy (more rotund and shorter than other strains), deviated from those normal characteristics of the Egyptian African catfish. Its meristic characters were also differed from those of other strains, assigned by reduced dorsal and anal fin rays number.
RESPONSE TO SELECTION FOR BODY WEIGHT IN THE THIRD GENERATION OF MASS SELECTION OF THE AFRICAN CATFISH (Clarias gariepinus) AT RESEARCH INSTITUTE FOR FISH BREEDING SUKAMANDI Iswanto, Bambang; Imron, Imron; Marnis, Huria; Suprapto, Rommy
Indonesian Aquaculture Journal Vol 11, No 1 (2016): (June 2016)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1787.817 KB) | DOI: 10.15578/iaj.11.1.2016.15-21

Abstract

Genetic improvement of the African catfish (Clarias gariepinus) in Indonesia for increasing growth performance has been conducted by Research Institute for Fish Breeding at Sukamandi through mass selection. Collection and characterizations of the founder populations, building the synthetic base population, first generation and second generation through mass selection were conducted during 2010-2013. Later, in 2014 it was followed by building the third generation. The present study aimed to find out the genetic gain in the third generation in term of response to selection for body weight. Fifty-two pairs of the selected (fast growing) individuals from the second generation were mated to produce the third generation. As a comparison, five pairs of average-sized individuals were mated to produce the control population, as a second generation representative. Larval rearing, nursery and grow-out phases were respectively held for 25 days in the aquaria, 30 days in the concrete ponds and 60 days in the concrete ponds. At the end of each phase, individual samplings of body weight were undertaken. The results showed that mean body weight of the third generation was higher than that of control population at the end of larval rearing phase (0.21 ± 0.26 g versus 0.20 ± 0.15 g), nursery phase (6.12 ± 2.93 g versus 5.80 ± 3.50 g) and grow-out phase (198.67 ± 82.82 g versus 165.22 ± 71.09 g). Those results revealed that response to selection for body weight of the third generation was positive, i.e. about 20.24% (33.45 g).
DISTRIBUTION AND EXPRESSION OF STRIPED CATFISH (Pangasionodon hypophtalmus) GROWTH HORMONE GENE (PhGH) IN THE ORGAN OF AFRICAN CATFISH (Clarias gariepinus) TRANSGENIC FOUNDER Marnis, Huria; Dewi, Raden Roro Sri Pudji Sinarni; Imron, Imron; Iswanto, Bambang
Indonesian Aquaculture Journal Vol 7, No 1 (2012): (June 2012)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (830.545 KB) | DOI: 10.15578/iaj.7.1.2012.11-18

Abstract

Faster growing African catfish can be produced by transgenesis. This study was conducted to investigate the distribution and expression of growth hormone gene (PhGH) in various organs of the transgenic African catfish (Clarias gariepinus) founder (F0). Transgene was detected using the PCR method in various organs, namely pituitary, brain, liver, heart, spleen, kidney, intestine, stomach, muscle, caudal fin, gill and eye. Transgene expression levels were analyzed using the method of reverse transcriptase-polymerase chain reaction (RT-PCR), -actin gene used as internal controls. The results showed that the PhGH was detected and expressed in all organs of the transgenic African catfish founder. The high level of PhGH expression was found in the liver, pituitary, intestine and brain; smaller amounts were detectable in muscle, spleen, kidneys, heart, and stomach, caudal fin, gill and eyes, range from 0.02-0.75 PhGH/-actin mRNA. The expression levels of PhGH had positive correlation with tissue and body size (P<0.05).
ASSOCIATION OF MICROSATELLITE GENETIC DIVERSITY WITH GROWTH RELATED TRAITS IN THE BASE POPULATION OF AFRICAN CATFISH, Clarias gariepinus, BREEDING PROGRAM Imron, Imron; Iswanto, Bambang; Ridzwan, Narita Syawalia; Suprapto, Rommy; Marnis, Huria
Indonesian Aquaculture Journal Vol 10, No 1 (2015): (June 2015)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (182.471 KB) | DOI: 10.15578/iaj.10.1.2015.1-11

Abstract

Genetic diversity at molecular level has been assumed to correlate with fitness related traits. However, accumulating evidences showed that the nature of that correlation has been variable. This study was aimed to explore the nature and possible mechanisms underlying that correlation by focusing on growth related traits in African catfish, Clarias gariepinus using microsatellite molecular markers. Fifty individual African catfish of 110 days-old were sampled and subjected to both morphological and molecular analyses. The standard length, total length and body weight as well as allelic scores of six microsatellite loci were measured on each individual. Indices of microsatellite diversity, namely individual multilocus heterozygosity (MLH) and mean microsatellite allelic distance (mean d2) for individual level, and mean observed heterozygosity (Ho) and single-locus heterozygosity (ho) for group level, were correlated to those traits using Pearson correlation coefficient (r). The Hardy Weinberg and linkage disequilibrium were carried out to explore the possible mechanisms underlying correlation. The results showed that at individual level the MLH and mean d2 were weakly correlated with standard length, (r=0.25, p<0.05) and (r=0.24, p<0.05), respectively. At group level, Ho was correlated with both standard and total length (r=0.99, p<0.05) while ho identified two loci, Cga03 and Cga06 significantly contributed to the correlation. Combining all relevant information, present study identified associative overdominance, both local effect and general effect hypotheses might responsible for the observed correlations.
MORPHOLOGICAL CHARACTERIZATION OF THE AFRICAN CATFISH (Clarias gariepinus BURCHELL, 1822) STRAINS INTRODUCED TO INDONESIA Iswanto, Bambang; Imron, Imron; Suprapto, Rommy; Marnis, Huria
Indonesian Aquaculture Journal Vol 10, No 2 (2015): (December 2015)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1969.444 KB) | DOI: 10.15578/iaj.10.2.2015.91-99

Abstract

African catfish (Clarias gariepinus Burchell, 1822) has become a great important species in Indonesian aquaculture. Several strains of the African catfish have been introduced to Indonesia for aquaculture purposes, initiated by Dumbo strain from Taiwan in 1985, followed by Paiton strain from Thailand in 1998, then Egypt strain from Egypt in 2007, Masamo strain from Thailand in 2010 and later Kenya strain from Kenya in 2011. Since its introductions, there were no reports yet on their characterization studies. The present study was conducted to morphologically characterize the strains of African catfish introduced to Indonesia, i.e. Dumbo, Paiton, Egypt, Masamo, and Kenya strains. Morphometric and meristic data obtained were analyzed using Principal Component Analysis. Results of the morphometric characterization in the present study revealed that Dumbo, Paiton, Masamo, and Kenya strains were indistinguishable, while morphometric characteristic of Egypt strain was more or less different from those of the other strains. On the other hand, results of the meristic characterization suggested that meristic characteristics of all strains of the introduced African catfish were not different from each other. Therefore, to keep the genetic purity of those introduced strains, they should be properly maintained in isolated places.
EXPRESSION OF GROWTH HORMONE (PhGH) GENE AND ANALYSIS OF INSULINE-LIKE GROWTH FACTOR I (IGF-I) PRODUCTION IN AFRICAN CATFISH (Clarias gariepinus) TRANSGENIC F-1 Marnis, Huria; Iswanto, Bambang; Suprapto, Rommy; Imron, Imron
Indonesian Aquaculture Journal Vol 8, No 2 (2013): (December 2013)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (655.581 KB) | DOI: 10.15578/iaj.8.2.2013.113-119

Abstract

We have previously produced F-1 transgenic of African catfish from crosses between founder transgenic female and non transgenic male. The aim of this study was to evaluate distribution and expression PhGH growth hormone gene transgenic African catfish organs and to measure the concentration of IGF-I in plasma. Transgene was detected using the PCR method in various organs, namely pituitary, brain, liver, heart, spleen, kidney, intestine, stomach, muscle, gill, and eye. Transgene expression levels were analyzed using the method of quantitative Reverse Transcriptase-Polymerase Chain Reaction (qRT-PCR). Plasma samples were analyzed for Insuline-like Growth Factor (IGF-I) using Enzyme Linked Immunosorbent Assay (ELISA) method. The results showed that the PhGH was detected and expressed in all organs of the transgenic African catfish (F-1). Liver exhibited the highest level of PhGH mRNA (23 x 106 copies). The plasma IGF-I levels in transgenic individuals were not significant than non transgenic. The higher level of exogenous PhGH gene expression may not represent the production of IGF-1.