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Journal : Biosaintifika: Journal of Biology

Aktivitas Antioksidan Jus Tomat pada Pencegahan Kerusakan Jaringan Paru-Paru Mencit yang Dipapar Asap Rokok (Genetic Diversity of Banana with B Genom Using Microsatelite Marker)

Biosaintifika: Journal of Biology & Biology Education Vol 1, No 1 (2009): March 2009
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

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Abstract

The activities of tomato juice’s antioxidant for the prevention of lung tissues damage in mice cigarette smoke induces endogen antioxidant is no longer effective on oxidative stress control towards cigarette consumer lung, thus exogenous antioxidant is a must. A kind of exogenous antioxidant is lycopen, a content of tomato. 40 mice is divided into 5 groups with 8 of each. Group I is the controlling group, group II is the negative control, and group III, IV, V are the group of mice that is supplied by tomato juice on the 1,7 g/100 g weight/day ; 3,4 g/100 g weight/day; and 5,1 g/100 g weight/day dozes. Each group, except the control one, is forced by cigarette smoke for 15 minutes per day in 54 days. On the 55th day, all mice is sacrificed. Specimens of their lungs are made in the way of histological by haematoxylin – eosin method. The data is about the comparison of lung tissue failed on each group, that is analyzed by Anava. The observation of each tissue on the group II showed emfisema pattern, that is alveolus membrane epithelia cell with no nucleus nor endothelium, with wide alveolus lumen and loose alveolus junction. The identical situation also found on group III. The failed tissue cannot be found on group IV and V. Antioxidant of tomato is capable of controlling lung tissue failed of mice of cigarette smoke. Keywords. Tomato juice, antioxidant, cigarette smoke, lung tissues

Chitosan as Chelating and Protective Agents from Lead Intoxication in Rat

Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

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Abstract

Increasing accumulation of lead in body causes serious health problems. Chelation is widely used to decrease lead level in body. This research aims the effectivity of chitosan in chelating lead and their protective ability toward liver and kidney for lead acetate-induced rat. Thirty rats were divided into 6 treatment groups. All groups, except control group, were administered by 175 mgkg-1 BW lead acetate. Positive control group was treated using EDTA 50 mgkg-1 BW. Treatment group 1,2, and 3 were treated using chitosan in dose of 64, 32, and 16 mgkg-1 BW dissolved in 2% acetic acid, respectively in 30 days. The effectivity of chitosan was compared to blood lead level. ALT and AST level were measured to determine the protective ability of chitosan. Normal function of kidney was assessed using creatinine level. Results showed that blood lead level from all treatment groups, except negative control group, had no significant difference from control group. EDTA and chitosan ability in chelating lead were proven by low level of AST, ALT, and creatinine in treatment groups. This indicated that there was no significant difference from control group. Chitosan capable of chelating lead and protecting kidney and liver from heavy metal.

Rambut Sebagai Bioindikator Pencemaran Timbal Pada Penduduk di Kecamatan Semarang Utara

Biosaintifika: Journal of Biology & Biology Education Vol 5, No 1 (2013): March 2013
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

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Abstract

Timbal (Pb) bersifat toksik, karsinogenik, bioakumulator dan biomagnifikasi. Bioakumulasi Pb dapat terjadi pada kuku, hati dan rambut. Penelitian bertujuan untuk mendeteksi apakah telah terjadi pencemaran timbal pada penduduk di kecamatan Semarang Utara dengan menggunakan rambut sebagai bioindikator. Penelitian deskriptif eksploratif ini menggunakan populasi seluruh penduduk di kecamatan Semarang Utara. Sampel diambil dengan teknik purposive sampling yaitu penduduk laki-laki berumur antara 17 sampai dengan 50 tahun yang tinggal di kelurahan Tanjung Mas dan Bandarharjo Semarang Utara, minimal selama 5 tahun terakhir terus menerus. Kadar timbal pada tubuh dideteksi dari kadar timbal yang terakumulasi pada rambut. Hasil penelitian menunjukkan terdapat kandungan timbal pada rambut 56 sampel. Kadar timbal tertinggi sebesar 17,028 ppm dan rata-rata 8,304 ppm. Penyebabnya diduga bukan berasal dari emisis kendaraan bermotor di jalan raya mengingat profesi sampel sebagian besar adalah nelayan, tetapi kemungkinan berasal dari air minum yang mereka konsumsi. Sampel air minum yang dikonsumsi terdeteksi mengandung timbal rata-rata 6 ppm. Simpulan dari penelitian ini telah terjadi pencemaran timbal pada sebagian penduduk Semarang Utara dengan tingkat ringan sampai sedang. Pencemaran diduga berasal dari air minum yang dikonsumsi.Lead (Pb) are toxic, carcinogenic, bioaccumulator and biomagnification. Pb bioaccumulation may occur in the nail, liver and hair. The research aimed to detect whether lead pollution has affected the people in North Semarang, using hair as bioindicator. The population of this explorative descriptive research was all residents of Sub-District of North Semarang. Samples were taken using purposive sampling technique, i.e. male residents of Tanjung Mas and Bandarharjo aged between 17 and 50 years, who have stayed in the sites for at least 5 years without interruption. The lead level in their body was detected from their hair strand using AAS (Atomic Absorption Spectrophotometery). Research showed that lead substance was detected in hair strands from 56 people. The highest level was 17.028 ppm and the average level was 8,304 ppm. It was suggested that the lead pollutant was not from motor vehicles emisions on the roads cause most samples were fishermen, instead it was suspected that the drinking water was the source of the lead. It was supported by the fact that the drinking water consumed daily by the residents has been contaminated by lead at the level of 6 ppm. Conclusion the lead pollution has affected some residents of North Semarang, ranging from low level to medium level. Presumably, the pollution was originated from the consumed drinking water.