Marianne Marianne
Faculty of Pharmacy, North Sumatera University

Published : 5 Documents
Articles

Found 5 Documents
Search

Penghambatan Degranulasi Mastosit Tersensitisasi Aktif oleh Curcuma Mangga Val. & Zijp Pada Mencit Secara In Vitro Alvianty, Fany; Mukhtar*, Rasmadin; Marianne, Marianne
Journal of Pharmaceutics and Pharmacology Vol 1, No 1 (2012)
Publisher : Journal of Pharmaceutics and Pharmacology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.105 KB)

Abstract

Background: Mastocyte widely recognized as critical effector cell in immune response is responsible for the occurrence of allergic reaction mediated by Imunoglobulin E (IgE). Mango ginger is a plant that the rhizome has an activity as antiallergy. Objective: To evaluate the effect of C. mangga rhizome ethanolic extract on the inhibition of active sensitized mastocyte degranulation of male mice in vitro. Methods: The inhibitory effect of mastocyte degranulation by Curcuma mangga was determined by calculating the number of mast cell which was not degranulated, derived from mice intraperitoneal fluid that were isolated, using hemocytometer. Before the test was taken, cell viability test had carried out to evaluate the citotoxicity of plant extract toward mastocytes. Results: The result showed that the ability of C. mangga dose 400 µg/ml showed percent of mast cell degranulation was smaller (30.18%) than C. mangga dose 200 µg/ml (42.76%), dose 100 µg/ml (55.76%), dose 50 µg/ml (75.85%), and dose 25 µg/ml (84.74%). It showed a relationship between dose excalation and percent of mastocyte degranulation that the increasing in dose will decrease the degranulation of mastocyte. Conclusion: C. mangga ethanolic extract can be used as antiallergic associated to the effect in decrease degranulation of active sensitized mastocyte. Keywords: degranulation, mastocyte, in vitro, Curcuma mangga Val. &Zijp ABSTRAK Latar belakang: Mastosit secara luas dianggap sebagai sel efektor penting dalam respon imun yang bertanggung jawab atas terjadinya reaksi alergi yang dimediasi oleh Imunoglobulin E (IgE). Curcuma mangga (temu mangga) merupakan salah satu tumbuhan yang rimpangnya diduga memiliki aktivitas sebagai antialergi. Tujuan: Untuk melihat pengaruh ekstrak etanol rimpang temu mangga terhadap penghambatan degranulasi mastosit yang tersensitisasi aktif pada mencit jantan secara in vitro. Metode: Efek penghambatan degranulasi mastosit oleh Curcuma mangga ditentukan dengan menghitung jumlah mastosit yang tidak terdegranulasi, yang berasal dari cairan intraperitoneal mencit yang diisolasi, menggunakan  alat  hemositometer. Sebelum dilakukan pengujian penghambatan degranulasi mastosit oleh C. mangga, dilakukan uji ketahanan sel (viability) untuk mengevaluasi sitotoksisitas ekstrak tanaman terhadap mastosit. Hasil: Hasil penelitian menunjukkan bahwa kemampuan C. mangga dosis 400 µg/ml menunjukkan persen degranulasi mastosit yang lebih kecil yaitu 30,18% dibandingkan dengan C. mangga dosis 200 µg/ml (42,76%), dosis 100 µg/ml (55,76%), dosis 50 µg/ml (75,85%), 25 µg/ml (84,74%). Hal ini menunjukkan bahwa adanya hubungan antara peningkatan dosis dengan persen degranulasi mastosit yaitu semakin besar peningkatan dosis maka mastosit yang terdegranulasi juga akan semakin sedikit. Kesimpulan: Ekstrak etanol C. mangga dapat digunakan sebagai anti alergi terkait dengan pengaruhnya dalam menurunkan degranulasi mastosit yang tersensitisasi aktif. Kata Kunci: degranulasi, mastosit, in vitro, Curcuma mangga Val. & Zijp 
ANTIDIABETIC ACTIVITY FROM ETHANOL EXTRACT OF KLUWIH’S LEAF (Artocarpus camansi) Marianne, Marianne; Yuandani, Yuandani; Rosnani, Rosnani
Jurnal Natural Volume 11, Number 2, September 2011
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.426 KB)

Abstract

Research on antidiabetic activity of ethanol leaf extracts Kluwih (Artocarpus camansi) has been carried out to determine the antidiabetic activity of ethanol extract of leaves kluwih (EELK) of mice using glucose tolerance test. The study began by dividing the mice into 6 groups. Group 1 was given 1% CMC, group 2 to group 4, given EELK 50, 100, 200, 400 mg / kg bw, respectively, and group 6  administered with glibenclamide 0.45 mg/kg bw. Thirty minutes later each group was given glucose 30 mg/kg bw. After 30 min of glucose loading, blood glucose levels of mice were measured, and measured again at minute 60, 90, 120 and 150 minutes. The results showed that the glucose tolerance test EELK a significant effect in lowering blood glucose levels compared to controls at 30 and 60 minutes after administration of glucose. At minute 90 to 150, the effect of blood glucose levels EELK restore back to normal. EELK demonstrated ability to reduce blood glucose levels, and EELK doses of 50 and 100 mg/kg bw showed antidiabetic effects better than a dose of 200 and 400 mg/kg bw. Increasing doses showed antagonistic effects: the ability to increase blood glucose levels.
Antidiabetic Activity of Leaves Ethanol Extract Chromolaena odorata (L.) R.M. King on Induced Male Mice with Alloxan Monohydrate Marianne, Marianne; Lestari, Dwi; Sukandar, Elin Yulinah; Kurniati, Neng Fisheri; Nasution, Rosnani
Jurnal Natural Volume 14, Number 1, March 2014
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (79.312 KB)

Abstract

Chromolaena odorata or Tekelan has been used traditionally to cope with diabetes mellitus, but the scientific evidence as antidiabetic agent has not been previously reported. Therefore, it is necessary to test the antidiabetic activity using ethanol extract of the tekelan leaves in male mice induced by alloxan. Mice were induced intravenously by alloxan dose of 55 mg/kg bw. After 7 days, mice were diabetic ( KGD > 200 mg/dL ) and were grouped into 6 groups: negative control group (only treated with CMC - Na) , positive control group (glibenclamide 0.45 mg/kg bw) and the 4 other groups were given ethanol extract of Tekelan leaves at doses of 5 , 25 , 125 and 250 mg/kg bw. Dosage administered for 28 days and blood glucose levels was checked every 7 days using a glucotest. Data were analyzed by one-way analysis of variation at 95% confidence level. Ethanol extract of tekelan’s leaves  at doses of 5 , 25 , 125 and 250 mg/kg bw can lower blood levels  from day 7 to day 28 and was significantly different compared to the negative control group ( p < 0.05). Ethanol extract of tekelan’s leaves at doses of 5 , 25 , 125 and 250 mg/kg bw showed antidiabetic activity in alloxan-induced mice.
STIMULASI WIRAUSAHA PADA MURID SEKOLAH MENENGAH ATAS DI TANJUNG PURA LANGKAT SUMATERA UTARA MELALUI WORKSHOP PEMBUATAN LOSION ANTI NYAMUK BERBASIS HERBAL Yuandani, Yuandani; Marianne, Marianne; Patilaya, Popi
ABDIMAS TALENTA Vol 2, No 1 (2017): Jurnal ABDIMAS TALENTA
Publisher : Universitas Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

SMA N 1 Tanjung Pura dan SMA Swasta Samanhudi terletak di kecamatan Tanjung Pura, Kabupaten Langkat, Provinsi Sumatera Utara. Kecamatan Tanjung Pura di Kabupaten Langkat merupakan salah satu kecamatan dengan tingkat kesejahteraan yang belum memadai. Sebahagian besar masyarakat bekerja sebagai petani dan nelayan, dan umumnya mereka belum dapat memenuhi biaya hidup yang memadai dan gagal dalam menguasai potensi produktif yang tersedia. Salah satu cara yang dapat dilakukan adalah diversifikasi pekerjaan dengan menstimulasi jiwa wirausaha generasi muda di Tanjung Pura, antara lain dengan memberikan keterampilan khusus yang nantinya bisa dimanfaatkan oleh mereka untuk membuka usaha sendiri. Salah satu cara yang akan dilakukan adalah dengan memberikan workshop pembuatan losion anti nyamuk. Hal ini mengingat kecamatan Tanjung Pura memiliki prevalensi yang tinggi terhadap penyakit malaria dan demam berdarah dengue (DBD), sehingga sangat tepat untuk mengajarkan para siswa SMA dalam pembuatan losion anti nyamuk agar dapat turut serta menurunkan angka kejadian penyakit tersebut. Oleh sebab itu,tujuan umum program pengabdian pada masyarakat ini adalah meningkatkan minat berwirausaha pada murid sekolah menengah atas, menurunkan angka pengangguran dan angka penyebaran penyakit malaria dan DBD di Kecamatan Tanjung Pura. Target utama dari program ini adalah sekelompok masyarakat yang memiliki latar pendidikan terakhir golongan menengah atau setara yang berada di Kecamatan Tanjung Pura. Secara khusus, program ini bertujuan untuk melatih siswa-siswi  setingkat SMA yang ada di SMAN 1 Tanjung Pura dan SMA Swasta Samanhudi dalam berwirausaha untuk memproduksi losion anti nyamuk yang berbahan dasar minyak sereh.
Total Phenolic Content of Ethanol Extract of Artrocarpus camansi Leave and its Effect to SOD (Superoxide Dismutase) Level in Mice Asrin, Hendri; Hasibuan, Poppy Anjelisa Zaitun; Marianne, Marianne
Indonesian Journal of Cancer Chemoprevention Vol 8, No 3 (2017)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (755.706 KB) | DOI: 10.14499/indonesianjcanchemoprev8iss3pp101-109

Abstract

A free radical is one of the triggers of degenerative diseases that become the biggest cause of death. Excessive production of free radicals can be neutralized by antioxidant. Antioxidants can be generated from within the body (intracellular). One of them by the enzyme SOD (superoxide dismutase). However, when the production of free radicals exceeds the ability of intracellular antioxidants to neutralize it, antioxidants from outside (extracellular) is necessary. The ethanol extract of Artocarpus camansi leaves (EEACL) contains phenolic compounds which has very strong antioxidant activity based on in vitro study using the DPPH method, but the in vivo study about the total phenolic content effect of its leaves toward antioxidant activity has not been done. 25 mice were divided into 5 groups consisting of control group, a group was induced by stress and three groups were induced by stress, but given EEACL with each dose of 50 mg/kg bw, 100 mg/kg bw and 150 mg/kg bw. Inducing stress in the form of psychological stress was carried out for 7 days and continued with the EEACL administration for 7 days. The mice were dissected and the livers were isolated, then the liver morphological was examined using Hematoxyllin Eosin (HE) staining method and SOD level was examined with immunohistochemical staining method. The data were analyzed with One Way ANOVA and Duncan test using SPSS program version 19.0. Total phenolic content of EEACL is 235.03 ± 4.306 mg GAE/ g of sample. The average SOD levels in the control group is 94.05 %, stressed group is 55.94 %, stress with EEACL dose 50 mg/kg bw group is 58.40 %, stress with EEACL dose 100 mg/kg bw group is 79.68 %, stress with EEACL dose 150 mg/kg bw group is 80.90 %. Based on statistical result, SOD level increased along with the increase of EEACL dose, but not significantly (p < 0,05). Total phenolic content of EEACL has an influence to SOD levels. SOD level increased along with the increase of EEACL administration dose. The higher dose of EEACL leading to higher levels of SOD in the mouse liver.Keywords : total phenolic content, superoxide dismutase, antioxidant, Artocarpus camansi