Elmy Mariana
Jurusan Peternakan, Fakultas Pertanian, Universitas Syiah Kuala

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Evaluasi Nilai Nutrisi dan Kecernaan In Vitro Pelepah Kelapa Sawit (Oil Palm Fronds) yang Difermentasi Menggunakan Aspergillus niger dengan Penambahan Sumber Karbohidrat yang Berbeda Wajizah, Sitti; Samadi, Samadi; Usman, Yunasri; Mariana, Elmy
Jurnal Agripet Vol 15, No 1 (2015): Volume 15, N0. 1, April 2015
Publisher : Agricultural Faculty

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(The evaluation of  nutritive value and In Vitro digestibility of oil palm fronds through fermentation by using  Aspergillus niger  with  different soluble carbohydrate sources) ABSTRACT. Oil palm frond (OPF) is one of potential sources of alternative feed, but has limited use due to high crude fiber and low crude protein contents. Fermentation is one of the methods widely applied to improve nutritive value of animal feed. The purpose of this research is to increase nutritive value of fermented oil palm fronds by adding different soluble carbohydrate source into fermentation media. The results of the experiments indicated that fermented oil palm fronds by Aspergillus niger had a significant effect (P<0,05)  on the content of crude protein, crude fiber, and ash. Generally, fermented oil palm fronds with different soluble carbohydrate was able to increase the content of crude protein of oil palm fronds, but not optimal yet in reducing the crude fiber content of fermented substrate. However, the addition of rice bran on the fermentation medium showed the best results, characterized by increasing crude protein and decreasing crude fiber contents as well as improved dry matter and organic matter digestibility, reflected in high concentration of VFA. 
Analisis Keragaman Gen Laktoferin Pada Sapi Friesian-Holstein Dengan Metode Pcr-Rflp Mariana, Elmy
Jurnal Agripet Vol 11, No 1 (2011): Volume 11, No. 1, April 2011
Publisher : Agricultural Faculty

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Polymorphism analysis of lactoferrin gene on holstein-friesian cow with PCR-RFLP methodABSTRACT. The purposes of this study were to identify the polymorphism of the lactoferrin gene in Holstein-Friesian (HF) cows. The study was conducted on 281 heads of HF lactating cows coming from dairy farmers in Lembang district. Investigation on variant genotypes of the lactoferrin gene used PCR-RFLP method. Genotyping of the lactoferrin gene with EcoRI restriction enzyme produced two genotypes, i.e. AA (65.5%) and AB (34.5%) genotypes.
Hubungan Polimorfisme Gen Laktoferin dengan Kualitas Susu pada Sapi Perah Friesian-Holstein Mariana, Elmy
Jurnal Agripet Vol 11, No 2 (2011): Volume 11, No. 2, Oktober 2011
Publisher : Agricultural Faculty

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Association of Lactoferrin Polymhorphism on Milk Yield and Milk Quality in Holstein-Friesian Dairy CowsABSTRACT. The purposes of this study were to identify the polymorphism of the lactoferrin gene and the association of genetic variants of the lactoferrin gene on milk quality in Holstein-Friesian (HF) cows. The study was conducted on 194 heads of HF lactating cows coming from semi intensive of small dairy farmers in two villages of the KPSBU Lembang, Lembang district. Investigation on variant genotypes of the lactoferrin gene used PCR-RFLP method. Genotyping of the lactoferrin gene with EcoRI restriction enzyme produced two genotypes, i.e. AA (65%) and AB (35%) genotypes. Both the AA and AB genotypes had inconsistency in controlling component of the milk quality. Further, the AB lactoferrin cows had a higher frequency in the classification of low somatic cells count (0.75), while the AA cows were at a higher frequency in the classification of high somatic cells count (0.60). It was concluded that there is no strong relationship between intron 6 lactoferrin gene with milk quality parameters. Further, the AB genotype of the lactoferrin gene had a consistency in producing lower somatic cells count compared to the AA genotype.
Pengaruh Suplementasi Tepung Terigu terhadap Pertumbuhan dan Laju Pengasaman Probiotik Lactobacillus acidophilus Mariana, Elmy; Susanti, Hilda
Jurnal Teknologi dan Industri Pertanian Indonesia Vol 4, No 3 (2012): Vol.(4) No.3, October 2012
Publisher : Agricultural Faculty

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This study aimed to determine the effect of the addition of wheat flour as a potential prebiotic on growth and acidification rate of probiotic Lactobacillus acidophilus (L. acidophilus) in fermented milk. The study was conducted by adding the flour with concentrations 1, 3 and 5% in the fermentation medium with 15% skim milk. Observed growth parameters including the total number of probiotic bacteria, decreasing pH and increasing acidity during the fermentation process. The results showed that the addition of wheat flour speed up the fermentation time, accelerate the process of decline in pH, increasing the acidity and increase the total number of probiotic bacteria.This study shows that wheat flour supplemented in fermented milk could enhance the growth characteristics of probiotics L. acidophilus. Keywords: probiotics, L. acidophilus, growth, wheat flour 
Respon Fisiologis dan Kualitas Susu Sapi Perah Friesian Holstein pada Musim Kemarau Panjang di Dataran Tinggi Mariana, Elmy; Hadi, Didik Nurul; Agustin, Nur Qoim
Jurnal Agripet Vol 16, No 2 (2016): Volume 16, No. 2, Oktober 2016
Publisher : Agricultural Faculty

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ABSTRAK. Tujuan dari penelitian ini adalah untuk mempelajari respon fisiologi, produksi dan kualitas susu sapi perah Frisian Holstein pada akhir musim kemarau panjang di Balai Pengembangan Ternak Sapi Perah dan Hijauan Makanan Ternak (BPT-SP HMT) Cikole, Lembang, Bandung.  Penentuan sampel menggunakan metode purposive sampling. Parameter mikroklimat yang diamati antara lain adalah temperatur lingkungan, kelembaban relatif, kecepatan angin, radiasi sinar matahari dalam kandang dan Temperature-Humidity Index (THI). Respon fisiologis yang diamati meliputi suhu rektal, suhu kulit, suhu tubuh, frekuensi respirasi dan denyut jantung. Nilai rerata THI (73.93±5.51) menunjukkan sapi perah berada dalam kondisi  stress ringan. Nilai rerata dari suhu rektal, suhu kulit, suhu tubuh, frekuensi respirasi dan denyut jantung secara berurutan 37.94±0.20°C; 32.15±1.25°C; 37.13±0.32°C; 39.13 ±3.00 dan 79.74±6.19. Nilai rerata persentase bahan kering, lemak dan protein susu secara berurutan 10,19 ± 0,72, 2,14 ± 0,38 dan 2,50 ± 0,32. Temperatur lingkungan yang tinggi  mempengaruhi respon fisiologis antara lain peningkatan denyut jantung yang lebih tinggi dari normal dan kualitas susu yang lebih rendah.   (Physiological responses and milk qualities of holstein friesian during long dry season at high altitude)ABSTRACT. The objectives of this study were to evaluate physiological responses and milk qualities of dairy cows in Balai Pengembangan Ternak Sapi Perah dan Hijauan Makanan Ternak (BPT-SP HMT) Cikole West Bandung at the end of long dry season. Samples were determined by using purposive sampling method. Microclimate parameters  were included  ambient temperature, relative humidity, air velocity, solar radiation and temperature-humidity index (THI). Physiological responses consisted of rectal temperature, skin temperature, body temperature, respiration rate and pulse rate. The mean value of THI (73.93±5.51) showed that dairy cows suffered by heat stress. The mean value of rectal temperature, skin temperature, body temperature, respiration rate and pulse rate were 37.94±0.20°C; 32.15±1.25°C; 37.13±0.32°C; 39.13±3.00 and 79.74±6.19 consecutively. The average percentage value of dry matter, fat and protein content in milk were 10.19±0.72, 2.14±0.38 and 2.50±0.32. High ambient temperature and low relative humidity affected physiological responses such as pulse rate that higher than normal,  and  lower milk yield.
Thermoregulation, Haematological Profile and Productivity of Holstein Friesian Under Heat Stress at Different Land Elevations mariana, Elmy; Sumantri, Cece; Astuti, Dewi Apri; Anggraeni, Anneke; Gunawan, Asep
Buletin Peternakan Vol 43, No 1 (2019): BULETIN PETERNAKAN VOL. 43 (1) FEBRUARY 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

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Abstract

The purpose of this research was to determine the effect of heat stress on thermoregulation, haematology, and productivity of Holstein Friesian (HF) dairy cows raised in different elevations. A total of 63 HF in a normal lactation period were used in this study. The research was conducted for 3 months during dry season in three different areas, which were at Pondok Ranggon (97 m.a.s.l) which categorized as a lowland, Ciawi (576 m.a.s.l) which categorized as a lower-upland, and Lembang (1241 m.a.s.l) which categorized as an upland. Observation on microclimate aspects which includes environmental temperature (Ta), relative humidity (RH) and Temperature-Humidity Index (THI) was done by recording each variable for every 2 hours starting from 08.00 to 16.00 WIB. The thermoregulation analysis was done based on the given physiological responses which consisted of the skin temperature (Ts), rectal temperature (Tr), body temperature (Tb), heart rate (Hr), respiratory rate (Rr), Heat Tolerance Coefficient (HTC), plasma cortisol level and haematological profile observation. The Ta, Rr, and THI measurements showed that in the lowland and lower-upland, the HF experienced moderate heat stress, while the HF raised in the upland area experienced less heat stress. The results showed that the dairy cows which raised in lowland had the highest HTC, Tr, Ts and Tb (P<0.05) and lowest Hr (P<0.05). All of the physiological and haematological parameters in the three study area showed a normal value.Furthermore, the erythrocyte, Hb and PVC concentration in a lowland raised HF were higher (P<0.05), while the plasma cortisol levels were not significantly different. The milk production of the observed dairy cows in different elevations was significantly different (P<0.05), with the highest milk yields, were found in the upland raised HF (13.1±3.52 kg), followed by the lower-upland (11.3±4.73 kg) and lowland (7.0±3.36 kg). In general, all of the HF raised in different land elevations was exposed to heat stress during dry seasons, even though the cows showed the ability to physiologically adapt and cope with the conditions. 
Mikroklimat, Termoregulasi dan Produktivitas Sapi Perah Friesians Holstein pada Ketinggian Tempat Berbeda Mariana, Elmy; Sumantri, Cece; Astuti, Dewi Apri; Anggraeni, Anneke; Gunawan, Asep
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 6, No 1 (2019): JITRO, Januari
Publisher : Universitas Halu Oleo

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ABSTRAKTujuan penelitian ini adalah untuk mengetahui pengaruh ketinggian tempat terhadap kondisi mikroklimat,termoregulasi dan produktivitas sapi Friesian-Holstein (FH). Penelitian dilakukan pada tiga lokasi dengan ketinggian berbeda, yaituPodok Ranggon (97mdpl), Ciawi (576mdpl), dan Lembang (1241mdpl). Sebanyak 63 sapi FH dalam kondisi laktasi normal digunakan dalam penelitian ini.  Aspek lingkungan meliputi suhu udara, kelembaban relatif dan Temperature-Humidity Index di dicatat setiap 2 jam dari pukul 08.00 sampai 16.00 WIB. Parameter termoregulasi yang diamati terdiri atas suhu kulit (Ts),suhu rektal (Tr), suhu tubuh (Tb), denyut jantung (Hr),laju pernapasan (Rr), dan Heat Tolerance Coeficient (HTC).Berdasarkan nilai Ta, Rr dan THI, dataran rendah memberikan dampak cekaman panas sedang, dataran sedangdan tinggi memberikan dampak cekaman panas ringan pada sapi perah. Hasil menunjukkan bahwa sapi dataran rendah memiliki Nilai HTC, Tr, Ts dan Tb tertinggi (P<0,05) dan Hr yang terendah (P<0,05). Produksi susu di ketiga lokasi penelitian berbeda nyata (P<0,05), dengan produksi susu tertinggi di dataran tinggi (13,1±3,52 kg),dataran sedang (11,3±4,73 kg) dan dataran rendah (7,0±3,36 kg). Secara umum sapi FH di dataran rendah, sedang dan tinggi tercekam panas akibat kondisi lingkungan yang berada pada kondisi di luar zona nyamanselama musim kemarau, akan tetapi mampu beradaptasi terhadap kondisi tersebut. Kata kunci: ketinggian tempat, produktivitas, sapi perah, cekaman panas, termoregulasiABSTRACTThe purpose of this research was to determine altitude effect on microclimate, thermoregulation and productivity of Friesian Holstein. Research was conducted in different altitudes, i.e.: Pondok Ranggon (97m asl), Ciawi (576m asl), and Lembang (1241 m asl). A total of 63 FH cows in normal lactation were used in this study. Microclimate aspects observation includes environmental temperature (Ta), relative humidity (RH) and Temperature-Humidity Index (THI) were recorded every 2 hours from 08.00 to 16.00. The physiological responses measurements consisted of skin temperature (Ts), rectal temperature (Tr), body temperature (Tb), heart rate (Hr), respiratory rate (Rr) and Heat Tolerance Coeficient (HTC). Lowland environmental provide moderate heat stress, while the medium and highland impacts with mild stress. The results showed that lowland cows have highest HTC, Tr, Ts and Tb (P<0.05) and lowest Hr (P<0.05). Milk production in the different altitude was significantly different (P<0.05), with the highest milk yields in the highlands (13.1±3.52 kg), medium (11.3±4.73 kg) and lowland (7.0±3.36 kg). In general, HF dairy cows in low-, medium- and highland are exposed to climatic stress during dry season conditions, although they have the ability to adapt physiologically and cope with environmental stress.  Keyworlds: altitude, dairy cattle, heat stress, productivity, physiological responses
Evaluasi Aspek Teknis Pemeliharaan Sapi Perah Menuju Good Dairy Farming Practices pada Peternakan Sapi Perah Rakyat Pondok Ranggon Anggraeni, Anneke; Mariana, Elmy
Jurnal Agripet Vol 16, No 2 (2016): Volume 16, No. 2, Oktober 2016
Publisher : Agricultural Faculty

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ABSTRAK. Peningkatan produktivitas ternak dapat dicapai melalui perbaikan genetik, pakan, manajemen dan modifikasi lingkungan. Penelitian ini bertujuan untuk mengevaluasi aspek teknis pemeliharaan sapi perah berdasarkan panduan Good Dairy Farming Practices (GDFP) pada manajemen pemeliharaan semi intensif di peternakan sapi perah rakyat Pondok Ranggon, Jakarta Timur. Aspek teknis pemeliharaan sapi perah yang dievaluasi meliputi aspek pemuliaan dan reproduksi, pakan, manajemen pemeliharaan, perkandangan, peralatan dan kesehatan ternak. Metode yang digunakan adalah survei, observasi dan pengukuran langsung. Data dianalisis secara deskriptif dan disajikan dalam bentuk frekuensi tabulasi untuk menggambarkan setiap karakteristik aspek pemuliaan dan reproduksi, pakan, manajemen pemeliharaan, perkandangan, peralatan dan kesehatan ternak. Hasil evaluasi aspek teknis yang dibandingkan dengan nilai rata-rata pelaksanaan GDFP di stasiun percontohan pemeliharaan sapi perah Pondok Ranggon menunjukkan bahwa pelaksanaan aspek teknis pemeliharaan sapi perah pada peternakan rakyat Pondok Ranggon termasuk dalam kategori cukup baik. Nilai rata-rata tertinggi pelaksana GDFP adalah pada aspek manajemen pemeliharaan, sementara aspek terendah adalah untuk kesehatan ternak. Kesimpulannya adalah pelaksanaan aspek teknis pemeliharaan sapi perah berdasarkan standar GDFP pada peternakan rakyat Pondok Ranggon harus ditingkatkan. (Technical aspects evaluation of dairy cow maintenance towards good dairy farming practices on pondok ranggon small holder dairy farm) ABSTRACT. Increasing livestock productivity can be achieved through genetic improvement, feeding, management and environmental modification. This study was aimed to evaluate various technical aspects in raising dairy cattle under semi intensive management at small dairy farmers in Pondok Ranggon (PR), Jakarta. Some technical aspects evaluated provided breeding, reproduction, feeding, management and health services. The methods used were by survey, observation and direct measurement. Data were analyzed descriptively then completed by tabulation frequencies to describe any characteristics of breeding decision, technical skills, daily management and health services. Evaluation on the considered technical aspects, compared to average values of Good Dairy Farming Practices (GDFP) showed that PR small dairy farmers in this study were categorized quite well. The highest average value of GDFP was for breeding and reproduction aspects, while the lowest one was for health services. It was concluded that PR small dairy farmers should be brought up for better management improvement.