Siti Muflichatun Mardiati
Jurusan Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro Jl. Prof. Soedarto, SH, Tembalang, Semarang

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DIAMETER DAN TEBAL LAPISAN EPITEL TUBULUS SEMINIFERUS SERTA BOBOT TESTIS MENCIT (Mus musculus) SETELAH PEMBERIAN TAUGE KACANG HIJAU (Vigna radiata) Anindita, Reza; Djaelani, M. Anwar; Mardiati, Siti Muflichatun
JURNAL ANATOMI FISIOLOGI Vol 17, No 2 (2009): Volume XVII, Nomor 2, Oktober 2009
Publisher : JURNAL ANATOMI FISIOLOGI

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Abstract

Young mung bean (Vigna radiata) believed as foodstuffs which fertility to defend potent on male. It reliable connected with to be vitamin E antioxidant essence which dominant by the side of other antioxidant as selenium and vitamin C which function in cell to protect from free radical reaction attack on spermatogenesis moment. Spermatogenesis activity on to occur testes inside at to direct seminiferous tubules. This experiment to aim learn tauge kacang hijau supply effect to be seminiferous tubules diameter, seminiferous tubules epithelium thick of layer and testes weight. Trial of animal which used is male rat to amount 10 tail, to divide appoint 2 group, Po as control and P1 is treatment which tauge kacang hijau to receive with oral dose 0,8 g/tail/day as long as 48 day. Observed which variable is seminiferous tubules diameter, seminiferous tubules epithelium thick of layer and testes weight. Acquired which data and then t-test using analyses with 95 % belief of level. Data analyses of result young mung bean supply effect to present not significanceof different to be seminiferous tubules diameter, seminiferous tubules epithelium thick of layer and testes weight treatment the mouse than control (P>0,05). Fact to present that young mung bean treatmen with most dominant vitamin E essence which antioksidant as function impotent spermatogenesis effect reliable in way.
Respons Biologis Puyuh setelah Pemberian Cahaya Monokromatik: suatu Kajian Kualitas Telur Mardiati, Siti Muflichatun; Kasiyati, Kasiyati; Irawati, Fika; Silalahi, Adonia B
JURNAL ANATOMI FISIOLOGI Vol 19, No 1 (2011) : Volume XIX, Nomor 1, Maret 2011
Publisher : JURNAL ANATOMI FISIOLOGI

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Light schedule is one of improvement in avian culture. The objective of the research was evaluate the potential of monochromatic light used to source artificial light for increased the egg quality. One hundred and twenty quails (Coturnix coturnix japonica) were divided into four treatments of lights, with thirty quails in each treatments. The treatments were red, green, and blue lights with intensity of 15 lux. Control treatment used yellow incandescent bulb of 15 watt. The red, green, and blue lights were provided by light emitting diodes (LED). All lights treatment were given for 14 h daily, started from 17.00 to 07.00. The result showed that quails exposed to monochromatic light significant (P<005) in abdominal fat, the body weight, feed consumption, triglyseride concentration, and the fat content in egg, and the egg weight, without significant effect in the egg protein. Blue light could be used to alternative in quail culture for improve the egg quality
Efek Penambahan Virgin Coconut Oil (VCO) terhadap Perkembangan Jengger dan Bobot Testis Ayam (Gallus sp.) Santoso, Mohammad Sigit; Tana, Silvana; Mardiati, Siti Muflichatun
JURNAL ANATOMI FISIOLOGI Vol 18, No 1 (2010): Vol. XVIII, No. 1, Maret 2010
Publisher : JURNAL ANATOMI FISIOLOGI

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Chicken is one of food sources that useful for human as a source of animal’s protein. Chicken has to produce good quality of meat and egg. One way to increase the product of chick is through the management of chicken food by adding Virgin Coconut Oil (VCO). The advantage of VCO is known as a safe product for human, therefore it will be safe for chicken too, there no doubt to consume the meat.The purpose of this research is to know about the effect of VCO related to the development of comb and testis weight. The sample of this research are           20 leghorn rooster. The method of this research is random sampling with four treatment and five repetition (Po: 0 cc/kg,   P1 : 1,5 cc/kg, P2 : 3 cc/kg, and P3 : 6 cc/kg). The data is obtained by using ANOVA with 95% significance. The result of data showed that there are not real different about the development of comb and testis weight’s chicken. It shows that the concentration of using VCO as additif feed to increase the productivity not successful yet.
Korelasi Jumlah Folikel Ovarium dengan Konsentrasi Hormon Estrogen Mencit (Mus musculus) setelah Konsumsi Harian Tepung Kedelai selama 40 Hari Mardiati, Siti Muflichatun; Sitasiwi, Agung Janika
JURNAL ANATOMI FISIOLOGI Vol 16, No 2 (2008): Vol. XVI, No. 2, Oktober 2008
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Soybeans contains phytoestrogen that influence reproductive process. Phytoestrogen administration withchronical dosage along growth periode cause the neuroendocrine system disturbance. Commonly in humanintake of phytoestrogen from soybeans or other soybeans product. So it is important to research beneficialand adverse effects of soybeans consumption with chronical dosage to reproductive process, particulary iscorelation between the sum of ovarium follicles and the estrogen level of mouse (Mus musculus) after dailyconsumption of soybeans along 40 days. The objective of this study was to know the corelation betweenthe sum of ovarium follicle and the estrogen level of mouse, by experimental design. Adult female micewere use as experimental animal, divided into two groups, there were control and treatment. Thegroup of treatment was administrated with 0,975 mg soybeans flour, it was contain ± 0,00156 mgphytoestrogen. The control group was administreted only drinking water. The result of this study showsthat were not significantly difference between control and treatment group. It concluded the corelationbetween the sum of ovarium follicles and the estrogen level were not influenced with daily consumption offitoestrogen.
Perbandingan Kadar Garam Natrium dan Kalium pada Tes Ferning Lendir Mulut Mardiati, Siti Muflichatun
JURNAL SAINS DAN MATEMATIKA Volume 15 Issue 1 Year 2007
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

ABSTRACT---The objective of this study was to know natrium and potassium levels of oral mucus ferning test. The object were 30 women, that were taken their oral mucus once within the 6th to 19th day of menstruation. The ferning test of oral mucus were examined with light microscope. The data distribution was analized by Kolmogorf – Smirnov, followed by non – parametric statistics. The mean, median and standard deviation were calculated with descriptive analysis. The Wilcoxon signed rank test was used to analyze the differentiation between the natrium and potassium levels of oral mucus in groups. The result of this study shows that the oral mucus potassium level is higher than natrium level. It means that potassium more important than natrium in the oral mucus ferning test.   Keywords : potassium, natrium, ferning score, oral mucus
Pengaruh Pemberian Vitamin A, B12, C dan Kombinasi Ketiganya Melalui Drinking Water Terhadap Panjang dan Bobot Tulang Femur, Tibia dan Tarsometatarsus Puyuh (Coturnix coturnix japonica L.) Setiawan, Erik; Praseno, Koen; Mardiati, Siti Muflichatun
JURNAL ANATOMI FISIOLOGI Vol 21, No 1 (2013): Volume XXI, Nomor 1, Maret 2013
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Abstract

Produktivitas puyuh (Coturnix coturnix japonica L.) yang meningkat merupakan pencapaian utama dari kegiatan beternak. Hal tersebut didukung oleh proses metabolisme yang baik, sehingga untuk menjaga proses tersebut agar berjalan dengan baik maka dibutuhkan vitamin pada ransum. Vitamin adalah zat yang dibutuhkan dalam jumlah sedikit oleh tubuh yang berpengaruh terhadap kesehatan tubuh serta dalam proses pertumbuhan, perkembangan dan reproduksi. Penelitian ini bertujuan untuk mendapatkan alternatif optimalisasi perkembangan dengan manajemen pemberian suplemen pada puyuh yaitu vitamin A, B12, C dan kombinasi ketiganya melalui drinking water. Perlakuan dimulai pada saat puyuh berumur 29 hari sampai umur 63 hari. Paramater dalam penelitian ini adalah panjang dan bobot tulang femur, tibia dan tarsometatarsus. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL), seluruh data dianalisis dengan Analysis of Varian (ANOVA). Hasil analisis yang berbeda nyata diuji dengan uji lanjut Jarak Berganda Duncan pada taraf kepercayaan 95%. Hasil penelitian menunjukkan bahwa pemberian larutan vitamin A, B12, C dan kombinasi ketiganya memberikan perbedaan tidak nyata terhadap panjang dan bobot tulang femur, tibia dan tarsometatarsus. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa pemberian larutan vitamin tersebut tidak berpotensi untuk memacu pertumbuhan dan perkembangan tulang puyuh.     Kata kunci : puyuh, vitamin, panjang dan bobot tulang
KADAR KOLESTEROL TELUR PUYUH SETELAH PEMBERIAN TEPUNG KUNYIT DALAM PAKAN Aviati, Vina; Mardiati, Siti Muflichatun; Saraswati, Tyas Rini
JURNAL ANATOMI FISIOLOGI Vol 22, No 1 (2014): Volume XXII, NOMOR 1, MARET 2014
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Abstract

The quail egg have complete nutrient content but its cholesterol level higher than chicken egg. This research was design to study the effect of turmeric powder supplementation to decline the cholesterol level of the quail egg. This experiment was designed by using the completely randomized design (CRD). The experimental animals were 45 female quails and   divided in to 3 groups, with 5 replications; there were P0 : as control, the group of the quail that given a standart concentrate,   P1 : the group of the quail with 54 mg/quail/day turmeric powder supplementation  before sexual maturity (from 14 days old during 1 month), and P2 : the group of quail with 54 mg/quail/day turmeric powder supplementation when sexual maturity onset (from 45 days old during 1 month). The cholesterol level of the egg sample of the quail at production in 4th month and its feed intake from each experimental group was analyzed by using analysis of variance (ANOVA) and followed by Duncan test. The result of this research showed that giving turmeric powder does not influence the feed intake and the cholesterol level of the quail egg which was given a treatment before sexual maturity, but that influence to increase the cholesterol level of quail egg that given turmeric powder when sexual maturity onset. It concluded that turmeric powder supplementation in food  not effective to decrease the cholesterol level of quail egg.
PERTUMBUHAN PUYUH (Coturnix coturnix japonica) SETELAH PEMBERIAN TEPUNG KUNYIT (Curcuma longa L.) PADA PAKAN Widyastuti, Wuri; Mardiati, Siti Muflichatun; Saraswati, Tyas Rini
JURNAL ANATOMI FISIOLOGI Vol 22, No 2 (2014): Volume XXII, NOMOR 2, OKTOBER 2014
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Abstract

Quail have the ability to grow and reproduce very quickly that at the age of 42 days. The aims of the research was to know the potential of turmeric powder as a feed additive to improve growth quail. This study used 60 quails consists of  4 treatments and 5 replications, namely P0 : as a control, a group of quail feed a standard concentrate, P1 : quail groups were given a dose of turmeric powder with 13,5 mg/quail/day, P2 : group quail were given a dose of turmeric powder with 27 mg/quail/day, and P3 : quail groups were given a dose of turmeric powder with 54 mg/quail/day. Each repeat consists of 3 quails are placed in a cage. Treatment begins at 3 weeks old quail until 2 month. Research using a completely randomized design. Parameters measured were feed consumption, water consumption, the length of the wing, the length of the femur, the length of  the tibia tarsus and the length of the tarso metatarsus. Data obtained from this study were analyzed using ANOVA ( Analysis of Varian ) at the level of 95 %. The result shows was not differences in feed consumption, water consumption, the length of the wing, the length of the femur, the length of the tibia tarsus and the length of the tarso metatarsus. Conclusion of this study  is the provision of turmeric powder in the feed does not have the potential to increase the growth of quail
Respon Histologis Hepar Tikus Wistar yang Mengalami Stres Fisiologis setelah Pemberian Pakan dengan Suplementasi Daging Ikan Gabus (Channa striata) Nugraheni, Beta Cahyaning; Sunarno, _; Mardiati, Siti Muflichatun
Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Volume 3, Nomor 2, Tahun 2018
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (651.095 KB) | DOI: 10.14710/baf.3.2.2018.173-180

Abstract

Stres fisiologis karena kekurangan nutrisi yang disertai aktivitas berlebihan dapat menyebabkan kekurangan energi yang dibutuhkan oleh tubuh. Kekurangan energi dapat berpengaruh pada penurunan bobot badan, bobot dan histologi hepar. Penelitian ini dilakukan untuk menganalisis pengaruh suplemen daging ikan gabus terhadap peningkatan bobot badan, bobot dan histologi hepar hewan uji yang mengalami stres fisiologis. Penelitian ini terdiri atas 5 perlakuan dan 4 kali ulangan. Perlakuan pada penelitian ini terdiri atas P0: tikus kontrol; P1, P2, P3, dan P4 berturut-turut adalah: suplemen daging ikan gabus dalam pakan dengan konsentrasi 5%; 10%; 15%; dan 20%. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Variabel yang diukur pada penelitian ini adalah bobot badan, bobot hepar, diameter vena sentralis dan hepatosit. Data dianalisis menggunakan Analysis of Variance (ANOVA) dan dilanjutkan dengan uji Duncan dengan signifikansi 5%. Hasil analisis data menunjukkan suplemen daging ikan gabus memberi pengaruh nyata terhadap bobot badan, bobot dan histologi hepar. Suplemen daging ikan gabus mampu meningkatkan bobot badan, bobot hepar, dan histologi hepar yang ditunjukkan dengan nilai diameter vena sentralis dan hepatosit lebih tinggi dibanding kontrol. Kesimpulan penelitian ini yaitu suplemen daging Channa striata dapat meningkatkan bobot badan, bobot hepar, diameter vena sentralis, dan hepatosit tikus Wistar dan konsentrasi 20% paling berpengaruh.
Bobot, Indeks Kuning Telur (IKT), Dan Haugh Unit (Hu) Telur Ayam Ras Setelah Perlakuan Dengan Pembungkusan Pasta Rimpang Temulawak (Curcuma xanthorrhiza Roxb). Putri, Dyah Ayu Maharani; Djaelani, Muhammad Anwar; Mardiati, Siti Muflichatun
Bioma Vol. 18, No.1, Tahun 2016
Publisher : Bioma

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma, 18, 2, 7-13

Abstract

Eggs are farm products that provide the greatest contribution to the achievement of community nutritional adequacy. The aims of the research was to an analyzed the quality of eggs in treatment without and with a temulawak rhizome pasta stored at 6,12, and 24 days. This research used  35 eggs consists of 7 treatment and 5 replication, namely PtT0 : as control, a group of eggs without packing temulawak rhizome pasta and without storage, PtT6 : a group of eggs without packing temulawak rhizome pasta after 6 days storage, PdT6 : a group of eggs with packing temulawak rhizome pasta after 6 days storage, PtT12 : a group of eggs without packing temulawak rhizome pasta after 12 days storage, PdT12 : a group of eggs with packing temulawak rhizome pasta after 12 days storage, PtT24 : a group of eggs without packing temulawak rhizome pasta after 24 days storage, and PdT24 : a group of eggs with packing temulawak rhizome pasta after 24 days storage. This research was analyzed used a completely randomized design (CRD). Variables measured were egg weight, yolk indeks, and Haugh Unit (HU). Data obtained from this study were analyzed using analysis of variance (ANOVA) if there is a real difference then continued further test using Duncan test at the level 95 %. The result  egg weight reducted and yolk index shows was significantly different in PtT6, PtT12, PtT24, and PdT24 and Haugh Unit (HU) shows was significantly different in PtT6, PtT12, PtT24. Conclusion of this research is gived temulawak rhizome pasta are able to maintain the quality of eggs were observed on days 6, 12, and 24. Key Word : Chicken egg,  Temulawak rhizome, Egg quality