Dewi Marbawati
Vector Borne Disease Control Research and Development Office in Banjarnegara

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BAKTERI LEPTOSPIRA PATOC I MERUPAKAN STRAIN BAKTERI DALAM KELOMPOK NON PATOGEN YANG SERING DITEMUKAN PADA PENDERITA LEPTOSPIROSIS Ikawati, Bina; Marbawati, Dewi
Balaba Vol 6, No 1 Jun (2010)
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Leptospirosis is one of zoonotic disease that needs serious attention from health practiciant and health programmer. This disease includes in the emerging infectious disease. Reseach from Loka Litbang P2B2 Banjarnegara in 2008 showed Leptospira of strain Patoc I (one of strain that include in non patogenic bacteria) found in 60,4% of 63 human blood sample that positive for Leptospira which Microscopic Aglutination Test (MAT). Literature studies on Leptospira bacteria strain Patoc I thus done about to know the reason why strain Patoc I usually found in Leptospirosis case, and wheter positive Patoc I in human spesimen indicates someone suffering from leptospirosis.Result showed Leptospira Sp strain Patoc I (Ames) or Patoc I (Paris) or Patoc I ATCC 23582 include in serovar Patoc, serogroup Semaranga, Genomospecies Leptospira biflexa, Genera Leptospira, family Leptospiraceae, ordo Spirochaetales, class Spirochaetes, Phylum Spirochaetes, Super Kingdom Bacteria. Patoc I usually showed cross reaction with many leptospira patogenic bacteria. Therefore Patoc I used as common indicator of Leptospirosis. Positive with Patoc I mostly indicates leptospirosis sufferer but probably from strain that not available in "batteray antigen test MAT" inlaboratory diagnosis. Research in laboratorium showed there was possibility of Patoc I genetic mutation.
PEMERIKSAAN LEPTOSPIROSIS SECARA LABORATORIS (HASIL PELATIHAN DI BALAI PENELITIAN VETEREINER BOGOR) Marbawati, Dewi; Ismanto, Hari
Balaba Vol 6, No 1 Jun (2010)
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Pelatihan pemeriksaan leptospirosis dilaksanakan dalam rangka meningkatkan kemampuan laboratoriurn bakteriologi yang ada di Loka litbang P2B2 Banjarnegara agar memiliki kemampuan melakukan pemeriksaan Leptospirosis. Beberapa materi yang diberikan dalam pelatihan ini diantaranya teori umum Leptospirosis dan pemeriksaan Leptospirosis secara laboratoris dengan metode MAT (Microscopic Aglutination Test). Pelatihan ini dilaksanakan mengingat bidang penelitian di Loka Litbang P2B2 Banjarnegara yang lebih fokus ke bidang penyakit bersumber rodensia, bahkan 3 tahun terakhir ini telah melakukan penelitian mengenai Leptospirosis.
TEKNIK ISOLASI - IDENTIFIKASI Yersinia pestis SEBAGAI PENYEBAB PENYAKIT PES (HASIL PELATIHAN DI BALAI BESAR VETERINER BOGOR) Marbawati, Dewi; Ismanto, Hari
Balaba Vol 6, No 2 Des (2010)
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Teknik isolasi dan identifikasi Y. pestis mempunyai prinsip- prinsip umum pertumbuhan yaitu terdiri dari tiga tahap yaitu: tahap pengkayaan, seleksi pada media agar dan uji biokimia. Tahap pengkayaan dilakukan dengan cara menimbang sebanyak 10-25 gram spesimen kemudian dimasukkan dalam blender atau plastik steril dan ditambah 90-225 ml media pengkayaan (dapat menggunakan Buffered Peptone Water (BPW), Brain Heart Infusion (BHI) atau menggunakan Nutrient Broth). Setelah itu dibuat suspensi spesimen 10%, kemudian dilakukan homogenisasi selama ± 2 menit dan diinkubasikan pada suhu 37 derajat Celcius selama 24 jam.
Hymenolepis sp, Cacing Pita Parasit Pada Tikus dan Manusia Marbawati, Dewi
Balaba Vol 6, No 2 Des (2010)
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Endoparasit pada tikus banyak macamnya. Hymenolepiphydae merupakan genus cacing pita yang biasa terdapat pada tikus. Cacing Pita (Cestoda) pada umumnya memiliki ciri tubuh terdiri dari rangkaian segmen-segmen yang masing-masing disebut proglotid. Kepala disebut skoleks dan memiliki alat isap (sucker) dan ada yang memiliki kait (rostellum) terbuat dari kitin. Pembentukan segmen (segmentasi) pada cacing pita disebut strobilasi.
KOLONISASI Mus musculus albino DI LABORATORIUM LOKA LITBANG P2B2 BANJARNEGARA Marbawati, Dewi; Ikawati, Bina
Balaba Vol 5, No 1 Jun (2009)
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The improvement and development of science and technologycal in health area must be followed with improvement of requirement of test animal would especially white mouse (Mus musculus albino/mencit). Usage of white mouse (mencit) because of relatively easy in handling, the size relatively small, the price relatively cheap, once birth can reach 16- 18, have similiarity in circulation system of human and doesnt have ability to vomit because having spillway in bouncing up, that is why white mouse used for drug trial research. This research expected to obtain stable white mouse colony in laboratory to fulfill requirement of white mouse as subject for research.Research was started in March-November 2008 in Loka Litbang P2B2 Banjarnegara. This research was elementary research with observational design. Data were collected from white mouse growth observation. Result of observation showed reproduction of white mouse after 5 month, from 6 white mouse increased become 17 (increased 2 times more), with number of deaths 11,76%. This white mouse survive until 12 months, but the average was 6 month. Oldest mencit until research ends (November 2008) ranges from 8 months. Daily observation data showed that white mouse can bear children until 6/pregnance. According to literature study mouse can bear children average 68/pregnance. Generaly baby mouse was around 1 gram, heavily horns depend on type (strain) of white mouse. Result of white mouse weight showed increase of white mouses body weight every 2 weeks was 8,6 grams. Observation data of development white mouse showed after 4 days the white mouse hair was seen, specially moustache that was clearly seen, on 5 day whole body was white. At the age of 10 days ear opened, part of other body like external mamilla and genitals become explains looked to be. At the age of 12 days eye started opens and active walking.
KOLEKSI REFERENSI NYAMUK DI DESA JEPANGREJO, KECAMATAN BLORA, KABUPATEN BLORA Marbawati, Dewi; Sholichah, Zumrotus
Balaba Vol 5, No 1 Jun (2009)
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Some kind of mosquitoes can transmit desease through their biting. Some of them are Anopheles that transmit malaria, Aedes aegypti and Aedes albopictus transmit Dengue Haemorrhagic Fever (DHF) and chikungunya, some of mosquitoes from Culex, Aedes, Anopheles and Mansonia genera that transmit filaria, etc. To study the mosquitoes diversity, the collection of mosquitoes in different species was needed. In order to increased the mosquitoes collection in Loka Litbang P2B2 Banjarnegara, collection in endemic area were conducted. One of selected area as the location of mosquitoes collection was Blora district. This survey was observational. Samples were taken with purposive sampling method that was all mosquitoes found during the study. Secondary data collected from health officer and public health center (puskesmas), observing the environment and social activities and also entomological survey. Result of entomological survey were various kind of mosquitoes, in Central Java Province, Blora district i.e.: Culex quinquefasciatus, Culex vishnui, Aedes aegypti, Anophelesvagus and Anopheles indefinitus.
PELATIHAN CARA UJI KLINIK YANG BAIK DI BADAN PENELITIAN DAN PENGEMBANGAN KESEHATAN DEPARTEMEN KESEHATAN RI Marbawati, Dewi; Sholichah, Zumrotus
Balaba Vol 5, No 1 Jun (2009)
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Materi pertama dalam acara pelatihan ini adalah mengenai Cara Uji Klinik yang Baik, prinsip ICH-GCP dan peran serta tanggung jawab Komisi Etik Penelitian Kesehatan (KEPK). Diskusi dilakukan di setiap akhir materi. Pada penjelasan materi Cara Uji Klinik yang Baik, dijelaskan mengenai beberapa fase uji klinik (clinical trial). Sedangkan dalam penjelasan materi prinsip ICH-GCP dijelaskan mengenai pentingnya Informed Consent Persetujuan Setelah Penjelasan (PSP) yang didasarkan pada penghargaan terhadap harkat manusia (respect for persons).
VIRUS DENGUE Marbawati, Dewi
Balaba Edisi 003 No.02/Tahun II Desember 2006
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VIRUS AVIAN INFLUENZA Marbawati, Dewi
Balaba Edisi 004 No.01/Tahun III Juni 2007
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Penyakit flu burung/Avian Influenza adalah penyakit yang disebabkan oleh virus yang secara alami menginfeksi bangsa burung. Penularan virus Avian Influenza bersifat spesifik, tetapi walaupun jarang dapat menyerang spesies hewan tertentu misalnya babi, kuda, harimau, macan tutul dan kucing. Penyakit ini sangat ditakuti karena memiliki kemampuan untuk menginfeksi orang dan dapat menimbulkan kematian. Pada unggas domestik, infeksi virus flu burung menyebabkan dua bentuk penyakit yang disebut sebagai patogenik rendah (Low Phatogenic Avian lnfluenza/LPAI) dan patogenik sangat tinggi (Highly Pathogenic Avian lnfluenza/HPAI). LPAI biasanya hanya menyebabkan gejala ringan, misalnya ditandai dengan bulu kasar atau produksi telur menurun, bahkan adakalanya tidak terdeteksi sama sekali adanya gejala. Di sisi lain, HPAI sangat patogen dan berakibat sangat fatal bagi unggas atau orang yang terinfeksi. Avian Influenza dapat menyebar dengan cepat di antara unggas dalam suatu peternakan. Daya patogeniknya menyerang organ dalam dengan berbagai variasi kerusakan jaringan yang tergantung pada derajat kerentanannya, serta mengakibatkan dampak morbiditas (angka kesakitan) dan mortalitas (angka kematian) tinggi. Angka kesakitan ataupun angka kematian dapat mencapai 90%-100%. Kematian unggas yang terserang terjadi dalam waktu singkat, sering hanya dalam waktu 24 jam atau kurang.
PENGKAJIAN BIONOMIK NYAMUK ANOPHELES SEBAGAI PENDEKATAN UNTUK MENGENDALIKAN POPULASINYA DALAM UPAYA MENANGGULANGI MALARIA (Studi kasus di Desa Kalibening, Kecamatan Sukoharjo, Kabupaten Wonosobo, Provinsi Jawa Tengah) Ikawati, SKM, Bina; Marbawati, Dewi
Balaba Edisi 006 No.01/Tahun IV Juni 2008
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A study on Bionomic of Anopheles mosquitoes as an approach to control the population of malaria (case studyat Kalibening Village, Sukoharjo Subdistrict, Wonosobo Regency, Central Java) had been conducted in July-October 2006. The objective of the study is to identify the bionomic of malaria vector. The result of this study revealed five species of Anopheles such as An. maculatus, An barbirostris, An. balabacensis, An. aconitus dan An. vagus. Three of them (An. aconitus, An. maculatus, An. balabacensis) represent as malarias vector in Java. Unfortunately these results were not supported by the ELISA test, since it proved that they are proven to be negative malaria vector. An. maculatus was found predominantly. The parous rate of An. maculatus was 39,89% (77 mortalities out of 193). Mosquoito age of An. maculatus ranged from 0.25-2.35 days. Thus, they were hardly to be a vectors. However, the situation might be different, under some circumstances, e.g. seasonal change. On the other way around, An. balabacensis and An. maculatus had a high density. An. Maculatus were mostly found in the ground pool around Salak plantation. Their bitting graphics (located at in door and out door) always occured at the same time. High in July then slowing down in Agust up to September and increased again in October whether those which were located in the cage were low in July, increasing in August up to September and slowing down in October. The peak density of An. maculatus which was biting on man occured at mid night (00. 00- 01. 00) and early in the morning (04. 00-05. 00) outdoors and indoors respectively. Key words : bionomic, Anopheles, malaria