Henky Manoppo
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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Journal : e-Journal BUDIDAYA PERAIRAN

Evaluation of The Use of Garlic (Allium sativum) in Enhancing Nonspecific Immune Response and Growth of Nile Tilapia (Oreochromis niloticus) Marentek, Gercyana; Manoppo, Henky; Longdong, Sammy N.J
e-Journal BUDIDAYA PERAIRAN Vol 1, No 1 (2013): Vol.1 No. 1 Januari 2013
Publisher : e-Journal BUDIDAYA PERAIRAN

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Abstract

The objective of this research was to examine the effect of garlic (Allium sativum) at different doses on nonspecific immune response and growth of nile tilapia (Oreochromis niloticus). Experimental fish was nile tilapia measuring 8-15 cm with an average weight of 10.4 g per individual. Before used in the experiment, fish were reared in fiber tank for two weeks for acclimatization. After acclimatization, the fish were moved into glass aquarium (50 cm x 30 cm x 30 cm) at the density of 15 fish/aquarium. Each aquarium was equipped with aerator. Five doses of garlic as treatments used in this research consisted of A= 0 g/kg diet, B= 10 g/kg, C= 20 g/kg, D= 30 g/kg and E= 40 g/kg. Fish was fed experimental diet at a dose of 5% of body weight per day, twice a day at 08.00 and 17.00. Data collected at the end of the experiment included immune parameters (TLC and phagocytosis activity) and growth of fish. ANOVA was used to evaluate the effect of treatments on immune response and growth of fish while Duncan Test was used to evaluate the different effect between treatments. Research result showed that after four weeks of feeding, TLC of fish fed diet supplemented with garlic significantly different (p<0,01) as compared to that of control fish. The highest number of TLC was observed at treatment C (15.413x107 cell.ml-1) followed by treatment B (15.39x107 cell.ml-1), D (13,920 x 107 cell.ml-1), E (13,582 x 107 cell.ml-1) and A (12,195 x 107 cell.ml-1). TLC of fish in treatment C was different significantly compared to control as well as to other treatments. Phagocytosis activity of fish in treatment C also increased significantly (p<0,01) compared to control. The highest PA was achieved in treatment C and then treatment B, D, E and A. Growth of fish in treatment C was different significantly compared to control fish as well as other treatments. As conclusion, oral administration of garlic at 20 g/kg diet could enhance nonspecific immune response and growth of nile tilapia. Key words: Garlic, Immune response, Total leukocyte count , Phagocytosis activity, Growth
Evaluation of Baker’s Yeast (Saccharomyces cereviciae) In Enhancing Non Specific Immune Response and Growth of Nile Tilapia Manurung, Usy N; Manoppo, Henky; Tumbol, Reiny A
e-Journal BUDIDAYA PERAIRAN Vol 1, No 1 (2013): Vol.1 No. 1 Januari 2013
Publisher : e-Journal BUDIDAYA PERAIRAN

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Abstract

This research was conducted to evaluate the efficacy of baker’s yeast in enhancing nonspecific immune response and growth of Nile tilapia. After two weeks of acclimatization in fiber tank, juveniles were put into glass aquarium at the density of 15 fish/ aquarium. Fish were fed pellet supplemented with baker’s yeast as treatment for four weeks at 5% of body weight per day, twice daily (08.00 and 17.00). Five doses of treatment used in this research were A (0 g yeast/kg pellet), B (10 g yeast/kg pellet), C (20 g yeast/kg pellet), D (30 g yeast/kg pellet), E (40 g yeast/kg pellet), Immune parameters included total leucocyte count (TLC) and phagocytosis activity were measured at the end of experiment. Fish growth was weighing at the end of research period. Anova was used to evaluate the effect of baker’s yeast on immune parameters and growth while to evaluate the different effect between treatment, Duncan test was used. Research result showed that after four week of feeding, TLC of fish fed diet supplemented with baker’s yeast significantly different compared to control fish. The highest TLC of fish was achieved in treatment B followed by treatment C. It was also found that phagocytosis activity of fish increased significantly in fish fed treatment diet with the highest PA was observed in treatment C. PA of fish in treatment C was different significantly compared to a treatment A, D and E but between treatment C and B, no significant difference was observed. Application of baker’s yeast in diet also significantly increased growth of fish (p=0,00). Fish fed diet supplemented with 20 g baker’s yeast/kg pellet has the highest weight gain compared to other fish in other treatments. It was concluded that the used of baker’s yeast at 20-30 g/kg pellet for four weeks could enhance nonspecific immune response and growth of Nile tilapia. Keywords: Saccharomyces cereviciae, total leucocyte count (TLC), Phagocytosis Activity, growth, Oreochromis niloticus
Peningkatan Respon Imun Non Spesik Ikan Mas (Cyprinus carpio L) Melalui Pemberian Bawang Putih (Allium Sativum) Lengka, Kedis; Manoppo, Henky; Kolopita, Magdalena E.F.
e-Journal BUDIDAYA PERAIRAN Vol 1, No 2 (2013)
Publisher : e-Journal BUDIDAYA PERAIRAN

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ABSTRACT This research aimed to determine the proper dose of white onion (Allium sativum) powder to stimulate the nonspecific immune response and growth of carp (Cyprinus carpio L). Fish (length 8-12 cm; weight 9.37 g in average) were obtained from BBAT Tatelu (Freshwater Aquaculture Board). After acclimatized in 1000-l fibre tank for two weeks, fish were introduced to 15 glass aquaria (80x40x40 cm) equiped with aeration at a density of 15 fish/aquarium. Fish were fed diet supplemented with white onion powder at five different doses (0, 10, 20, 30 and 40 g/kg diet), twice daily at 5%bw. Data collected included total leucocyte count (TLC), phagocytosis activity and growth of fish. Results showed that after four weeks of feeding, supplementation of white onion powder at 30 g/kg diet was effective to enhance nonspecific immune response and growth of carp. Keywords: Allium sativum, immune response, total leucocyte count, phagocytosis activity
Respon imun nonspesifik dan pertumbuhan udang vaname (Litopenaeus vannamei) yang diberi pakan yang ditambahkan nukleotida dengan lama pemberian berbeda Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 2, No 1 (2014)
Publisher : e-Journal BUDIDAYA PERAIRAN

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Abstract This study evaluated the effects of feeding protocol of nucleotide-supplemented diet on total hemocytes, PO activity and growth of whiteleg shrimp.  Shrimp juveniles were reared in five 120-l glass aquaria at the density of 25 juveniles each.  Shrimp pellet was supplemented with nucleotides mixture at 400 mg.kg-1 diet. Shrimps were fed three time a day at 3%/bw/d.  Feeding protocol was 7 days nucleotides diet – 7 days basal diet alterably for 49 days.  Total hemocyte count, PO activity, and growth were measured at the end of experiment.  Research result showed that oral administration of nucleotides at seven days interval did not affect THC, PO activity, and growth of shrimp. But if the feed was administered successively for four weeks, supplementation of nucleotides would significantly enhance the nonspecific immune response and shrimp growth.   Keywords: Litopenaeus vannamei, nucleotides, PO activity, total hemocyte count
Penggunaan ragi roti (Saccharomyces cereviciae) secara in situ untuk meningkatkan respon kebal non-spesifik ikan nila (Oreochromis niloticus) Rawung, Meilina E; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 2, No 2 (2014)
Publisher : e-Journal BUDIDAYA PERAIRAN

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Abstract

The objetive of research was to examine the effect of yeast cell Saccharomyces cereviciae on non-specific immune response of nile tilapia, Oreochromis niloticus. Juveniles weighing 31.83 g in average were cultured in five concrete tanks with a density of 30 fish per tank. The fish were fed pellet supplemented with yeast cell at five different doses (0, 10, 20, 30, 40 g/kg pellet) for three consecutive weeks as much as 4%/bb/day, twice daily. At the end of feeding, three fish from each tank were sampled to measure the immune parameters namely total leucocyte count and phagocytosis activity of phagocyte cells. Research results showed that after three weeks of feeding, total leucocyte and phagocytosis activity of fish fed pellet supplemented with yeast cells were significantly different as compared to those of control fish.  The highest total leucocyte and phagocytosis activity were achieved in fish fed pellet supplemented with 10 g of yeast cells/kg pellet.  It was concluded that the use of yeast cells for three weeks could enhance the nonspecific immune response of nile tilapia. Keywords: Saccharomyces cerreviciae, nonspecific immune response, total leucocyte count, phagocytosis activity, nile tilapia, Oreochromis niloticus
Respon imun krustase Manoppo, Henky; Kolopita, Magdalena E.F
e-Journal BUDIDAYA PERAIRAN Vol 2, No 2 (2014)
Publisher : e-Journal BUDIDAYA PERAIRAN

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Abstract

Crustacean does not have adaptive immune system and mostly depends on innate or nonspecific immune response. This system can recognize and destroy foreign materials including pathogen.  The nonspecific immune responses of crustacean consist of cellular and humoral responses.  In cellular response, hemocyte plays an important role in body defenses to pathogen including virus, bacteria, fungi, and parasites. In humoral response, recognition of pathogen is mediated by protein and enzyme such as β-1,3-glucan-binding protein (BGBP),  lipopolysaccharide-binding protein (LPS-BP), peptidoglycan recognition protein, phenoloxydase enzime. Keywords: crustacean, nonspecific immune response, cellular responses, humoral response, protein, enzime
Peningkatan Pertumbuhan Ikan Nila (Oreochromis niloticus) Melalui Pemberian Immunostimulan Ragi Roti (Saccharomyces cereviciae) Bugis, Achmad A; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 2, No 3 (2014)
Publisher : e-Journal BUDIDAYA PERAIRAN

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Abstract

This research aimed to examine the effect of baker’s yeast as immunostimulant on growth of nile tilapia and to find out a proper dose of baker’s yeast that promoted optimal growth of fish. Juveniles with an average weight of 31.83 g were obtained from stock available at Aquaculture Study Program of the Faculty of Fisheries and Marine Sciences.  This research was conducted at the experimental ponds located at the Faculty of Fisheries and Marine Sciences for three months. During the experiment, fish was fed with pellet supplemented with baker’s yeast as treatment for four weeks as much as 4%/bw/day, twice daily.  The doses of baker’s yeast consisted of 0, 10, 20, 30, and 40 g/kg pellet. Fish growth including absolute and relative growth was measured at two weeks interval.  Research results showed that  after two weeks of feeding, supplementation of baker’s yeast did not affect the growth of fish but after four weeks of feeding, supplementation of baker’s yeast significantly increased fish growth. Fish fed pellet supplemented with 10 g baker’s yeast displayed the highest growth. As conclusion, supplementation of baker’s yeast in fish food could enchance the growth of fish.   Keywords:  Oreochromis niloticus, immunostimulant, baker’s yeast, growth
Kelangsungan Hidup Larva Ikan Nila yang Berasal Dari Induk yang Diberi Pakan Berimunostimulan Maleko, Ayu; Sinjal, Hengky J; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 2, No 3 (2014)
Publisher : e-Journal BUDIDAYA PERAIRAN

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The objective of this research was to evaluate the effect of feed supplemented with baker’s yeast on survival rate of nile tilapia larvae and to determine the effective dose of baker’s yeast that could optimally improve larval quality.  The research was conducted at Pathology and Clinic of Fish Disease from June – September 2014.  Fish as brood stock candidates was cultivated at five concrete tanks (2x1x1 m3) at a density of 30 fish per tank.  Each tank was equipped with water inlet, outlet and water pump for recirculating and aerating.  After seven days of adaptation, fish was fed pellet supplemented with baker’s yeast at five different doses (0, 10, 20, 30, 40 g/kg food) for four consecutive weeks at 4%/bb/day, twice daily.  Larva was captured with seser and moved into five glass aquaria with density of 20 larvae/aquarium and reared for two weeks.  Data collected was survival rate of larvae achieved until the end of larval rearing.  Research result showed that larvae produced by brood stock fed food supplemented with 10 g baker’s yeast per kg food had the highest survival rate namely 60%.  As conclusion, supplementation of baker’s yeast into brood stock feed might increase survival rate of larvae.   Keywords:  nile tilapia, baker’s yeast, larvae, survival rate
Identifikasi Penyakit Bakterial Pada Benih Sidat (Anguilla marmorata) di Balai Budidaya Air Tawar Tatelu Kusen, Kevin Octavian; Tumbol, Reiny A; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 3, No 1 (2015)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (88.624 KB) | DOI: 10.35800/bdp.3.1.2015.6935

Abstract

This study aimed to identify the bacterial disease in cultured eel elvers (Anguilla marmorata). The samples used for this study were taken from the Tatelu Freshwater Aquaculture Center. The samples taken were elvers (juvenile life stage of A. marmorata)  which showed clinical symptoms of bacterial infection such as bleeding in the chest, abdomen, and the base of the fin, passive movement, weakness, and loss of balance of the body, loss of appetite, some wounds on back fin, chest, and tail, and the body was not slippery/shiny. Three elvers with the size of 4-6 cm, and weigh about 0.2 g were taken as samples. The organ chosen to be targeted for isolation was head kidney. Each sample was isolated twice as replicates and streaked on Triptic Soy Agar (TSA) medium. First replicates were identified in the Tatelu Health Lab of Freshwater Aquaculture, and the second replicates were identified in North Sulawesi Provincial Health Lab. Bacterial identification was done through a series of observations and morphology of bacterial colonies through gram stain, followed by biochemical tests through oxidase test, catalase test, Triple Sugar Iron Agar (TSIA) test, H2S production test, motility test, O/F test, and Simmons Citrate Agar (SCA) test. Water quality parameters that were measured included temperature, disolve oxygen, pH, and ammonia. Based on identification of bacteria in cultured elvers in Tatelu Freshwater Aquaculture Center, the elver samples had been infected with bacterial  Aeromonas hydrophila. Keywords: elver, identification, Aeromonas hydrophila, Tatelu Freshwater Aquaculture Center
Penggunaan ragi roti (Saccharomyces cerevisiae) sebagai imunostimulan untuk meningkatkan resistensi ikan mas (Cyprinus carpio L) terhadap infeksi bakteri Aeromonas hydrophila Manoppo, Henky; Kolopita, Magdalena E.F.
e-Journal BUDIDAYA PERAIRAN Vol 4, No 3 (2016)
Publisher : e-Journal BUDIDAYA PERAIRAN

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A research had been conducted to evaluate the use of baker’s yeast as immunostimulant in enhancing the resistance of carp (Cyprinus carpio L) to Aeromonas hydrophila infection.  Experimental fish were obtained from Freshwater Aquaculture Board in Talelu, Minahasa Regency, and transported to the Laboratory of Aquaculture Technology at the Faculty of Fisheries and Marine Science, Sam Ratulangi University. During two weeks of  acclimatization process, fingerlings were fed with commercial feed (pellet) at 5% of body weight per day, twice a day.  After acclimatization, fish was randomly distributed into 15 aquaria with a density of 25 fishes/aquarium. The doses of yeast cells as treatments included 5, 10, 15 and 20 g yeast/kg of pellet while control pellet not supplemented with yeast cell. Fish was fed with treatment pellets for four consecutive weeks at 5% of body weight per day,  twice daily at 09.00 am and 16.00 pm. Afterward, the density of fish were adjusted to 10 fish/aquarium. Fish were then challenged with A. hydrophila through intraperitoneal injection of  0.2 mL of A. hydrophila suspension containing 1x107 cfu/mL. Research result showed that supplementation of yeast cells into fish pellet significantly influenced the resistance of fish to A. hydrophila infection (p<0,01). The highest resistance were observed in fish fed pellet supplemented with 5 g yeast/kg of pellet. Thus, supplementation of  baker’s yeast cells into commercial fish feed was able to increase  resistance of carp to A. hydrophila infection.   Keywords: baker’s yeast, Cyprinus carpio, A. hydrophila, resistance, intraperitoneal injection