Henky Manoppo
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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Evaluation of The Use of Garlic (Allium sativum) in Enhancing Nonspecific Immune Response and Growth of Nile Tilapia (Oreochromis niloticus) Marentek, Gercyana; Manoppo, Henky; Longdong, Sammy N.J
e-Journal BUDIDAYA PERAIRAN Vol 1, No 1 (2013): Vol.1 No. 1 Januari 2013
Publisher : e-Journal BUDIDAYA PERAIRAN

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The objective of this research was to examine the effect of garlic (Allium sativum) at different doses on nonspecific immune response and growth of nile tilapia (Oreochromis niloticus). Experimental fish was nile tilapia measuring 8-15 cm with an average weight of 10.4 g per individual. Before used in the experiment, fish were reared in fiber tank for two weeks for acclimatization. After acclimatization, the fish were moved into glass aquarium (50 cm x 30 cm x 30 cm) at the density of 15 fish/aquarium. Each aquarium was equipped with aerator. Five doses of garlic as treatments used in this research consisted of A= 0 g/kg diet, B= 10 g/kg, C= 20 g/kg, D= 30 g/kg and E= 40 g/kg. Fish was fed experimental diet at a dose of 5% of body weight per day, twice a day at 08.00 and 17.00. Data collected at the end of the experiment included immune parameters (TLC and phagocytosis activity) and growth of fish. ANOVA was used to evaluate the effect of treatments on immune response and growth of fish while Duncan Test was used to evaluate the different effect between treatments. Research result showed that after four weeks of feeding, TLC of fish fed diet supplemented with garlic significantly different (p<0,01) as compared to that of control fish. The highest number of TLC was observed at treatment C (15.413x107 cell.ml-1) followed by treatment B (15.39x107 cell.ml-1), D (13,920 x 107 cell.ml-1), E (13,582 x 107 cell.ml-1) and A (12,195 x 107 cell.ml-1). TLC of fish in treatment C was different significantly compared to control as well as to other treatments. Phagocytosis activity of fish in treatment C also increased significantly (p<0,01) compared to control. The highest PA was achieved in treatment C and then treatment B, D, E and A. Growth of fish in treatment C was different significantly compared to control fish as well as other treatments. As conclusion, oral administration of garlic at 20 g/kg diet could enhance nonspecific immune response and growth of nile tilapia. Key words: Garlic, Immune response, Total leukocyte count , Phagocytosis activity, Growth
Evaluation of Baker’s Yeast (Saccharomyces cereviciae) In Enhancing Non Specific Immune Response and Growth of Nile Tilapia Manurung, Usy N; Manoppo, Henky; Tumbol, Reiny A
e-Journal BUDIDAYA PERAIRAN Vol 1, No 1 (2013): Vol.1 No. 1 Januari 2013
Publisher : e-Journal BUDIDAYA PERAIRAN

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This research was conducted to evaluate the efficacy of baker’s yeast in enhancing nonspecific immune response and growth of Nile tilapia. After two weeks of acclimatization in fiber tank, juveniles were put into glass aquarium at the density of 15 fish/ aquarium. Fish were fed pellet supplemented with baker’s yeast as treatment for four weeks at 5% of body weight per day, twice daily (08.00 and 17.00). Five doses of treatment used in this research were A (0 g yeast/kg pellet), B (10 g yeast/kg pellet), C (20 g yeast/kg pellet), D (30 g yeast/kg pellet), E (40 g yeast/kg pellet), Immune parameters included total leucocyte count (TLC) and phagocytosis activity were measured at the end of experiment. Fish growth was weighing at the end of research period. Anova was used to evaluate the effect of baker’s yeast on immune parameters and growth while to evaluate the different effect between treatment, Duncan test was used. Research result showed that after four week of feeding, TLC of fish fed diet supplemented with baker’s yeast significantly different compared to control fish. The highest TLC of fish was achieved in treatment B followed by treatment C. It was also found that phagocytosis activity of fish increased significantly in fish fed treatment diet with the highest PA was observed in treatment C. PA of fish in treatment C was different significantly compared to a treatment A, D and E but between treatment C and B, no significant difference was observed. Application of baker’s yeast in diet also significantly increased growth of fish (p=0,00). Fish fed diet supplemented with 20 g baker’s yeast/kg pellet has the highest weight gain compared to other fish in other treatments. It was concluded that the used of baker’s yeast at 20-30 g/kg pellet for four weeks could enhance nonspecific immune response and growth of Nile tilapia. Keywords: Saccharomyces cereviciae, total leucocyte count (TLC), Phagocytosis Activity, growth, Oreochromis niloticus
DETERMINASI MOLEKULER KOI HERPES VIRUS (KHV) YANG DIISOLASI DARI IKAN KOI (Cyprinus carpio koi) Saselah, Jetti Treslah; Tumbol, Reiny A; Manoppo, Henky
JURNAL PERIKANAN DAN KELAUTAN TROPIS Vol 8, No 2 (2012)
Publisher : Sam Ratulangi University

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Penelitian ini bertujuan untuk mendeterminasi keberadaan Koi Herpes Virus (KHV) pada ikan koi (Cyprinus carpio koi). Sampel ikan diambil dari Kabupaten Kepulauan Sangihe. Penelitian di lakukan di Laboratorium Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sam Ratulangi dengan menggunakan metode Polymerase Chain Reaction (PCR). Hasil pemeriksaan PCR terhadap sampel ikan koi mengindikasikan bahwa sampel ikan koi telah terinfeksi oleh virus KHV, yang ditandai dengan munculnya pita DNA pada hasil visualisasi elektroforesis agarosa. Hasil pemeriksaan pada morfologi ternyata sampel ikan yang digunakan menunjukkan gejala-gejala klinis terserang KHV seperti mata pucat, insang berwarna pucat serta produksi lendir yang berlebihan. Beberapa sampel lainnya walaupun secara morfologi belum menunjukan gejala-gejala klinis tetapi melalui pemeriksaan PCR telah terindikasikan terinfeksi KHV. Kata kunci: KHV, PCR, ikan koi   The purpose of this study was to determine the presence of Koi Herpes Virus (KHV) in Koi (Cyprinus carpio koi). Fish samples were taken from the Sangihe Archipelago Regency. Laboratory work conducted at the Laboratory of Biology Faculty of Mathematics and Natural Sciences University of Sam Ratulangi using Polymerase Chain Reaction (PCR) method. The samples indicated that the koi fish samples were infected with KHV. It is shown by the appearance of DNA bands on agarose electrophore­sis visualization. The morphological examination of the body indicated clinical symptoms of KHV infection, such as pale eyes, pale gills and excessive mucus production. Several other samples, although without clinical symptoms, have shown to be infected as indicated by PCR test. Keywords: KHV, PCR, Koi.
Enhancement of non-specific immune response, resistance and growth of (Litopenaeus vannamei) by oral administration of nucleotide Manoppo, Henky; Sukenda, .; Djokosetiyanto, Daniel; Sukadi, Mochamad Fatuchri; Harris, Enang
Jurnal Akuakultur Indonesia Vol 10, No 1 (2011): Jurnal Akuakultur Indonesia
Publisher : Jurnal Akuakultur Indonesia

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This research evaluated the nonspecific immune responsse, resistance, and growth of Litopenaeus vannamei fed nucleotide diet. Shrimp juveniles (mean weight 5.39±0.56 g) were reared in two groups of glass aquaria, each with three replications. Shrimps in group one and group two were fed nucleotide diet and basal diet each for four weeks. Total haemocyte count (THC) and PO activity were evaluated at the end of feeding while growth was measured at two weeks interval. At the end of feeding trial, the shrimps were intramuscularly injected with Vibrio harveyi 0.1x106 cfu.shrimp-1. THC of shrimp fed nucleotide diet significantly increased (P
Peningkatan Respon Imun Non Spesik Ikan Mas (Cyprinus carpio L) Melalui Pemberian Bawang Putih (Allium Sativum) Lengka, Kedis; Manoppo, Henky; Kolopita, Magdalena E.F.
e-Journal BUDIDAYA PERAIRAN Vol 1, No 2 (2013)
Publisher : e-Journal BUDIDAYA PERAIRAN

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ABSTRACT This research aimed to determine the proper dose of white onion (Allium sativum) powder to stimulate the nonspecific immune response and growth of carp (Cyprinus carpio L). Fish (length 8-12 cm; weight 9.37 g in average) were obtained from BBAT Tatelu (Freshwater Aquaculture Board). After acclimatized in 1000-l fibre tank for two weeks, fish were introduced to 15 glass aquaria (80x40x40 cm) equiped with aeration at a density of 15 fish/aquarium. Fish were fed diet supplemented with white onion powder at five different doses (0, 10, 20, 30 and 40 g/kg diet), twice daily at 5%bw. Data collected included total leucocyte count (TLC), phagocytosis activity and growth of fish. Results showed that after four weeks of feeding, supplementation of white onion powder at 30 g/kg diet was effective to enhance nonspecific immune response and growth of carp. Keywords: Allium sativum, immune response, total leucocyte count, phagocytosis activity
Respon imun nonspesifik dan pertumbuhan udang vaname (Litopenaeus vannamei) yang diberi pakan yang ditambahkan nukleotida dengan lama pemberian berbeda Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 2, No 1 (2014)
Publisher : e-Journal BUDIDAYA PERAIRAN

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Abstract This study evaluated the effects of feeding protocol of nucleotide-supplemented diet on total hemocytes, PO activity and growth of whiteleg shrimp.  Shrimp juveniles were reared in five 120-l glass aquaria at the density of 25 juveniles each.  Shrimp pellet was supplemented with nucleotides mixture at 400 mg.kg-1 diet. Shrimps were fed three time a day at 3%/bw/d.  Feeding protocol was 7 days nucleotides diet – 7 days basal diet alterably for 49 days.  Total hemocyte count, PO activity, and growth were measured at the end of experiment.  Research result showed that oral administration of nucleotides at seven days interval did not affect THC, PO activity, and growth of shrimp. But if the feed was administered successively for four weeks, supplementation of nucleotides would significantly enhance the nonspecific immune response and shrimp growth.   Keywords: Litopenaeus vannamei, nucleotides, PO activity, total hemocyte count
Penggunaan ragi roti (Saccharomyces cereviciae) secara in situ untuk meningkatkan respon kebal non-spesifik ikan nila (Oreochromis niloticus) Rawung, Meilina E; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 2, No 2 (2014)
Publisher : e-Journal BUDIDAYA PERAIRAN

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The objetive of research was to examine the effect of yeast cell Saccharomyces cereviciae on non-specific immune response of nile tilapia, Oreochromis niloticus. Juveniles weighing 31.83 g in average were cultured in five concrete tanks with a density of 30 fish per tank. The fish were fed pellet supplemented with yeast cell at five different doses (0, 10, 20, 30, 40 g/kg pellet) for three consecutive weeks as much as 4%/bb/day, twice daily. At the end of feeding, three fish from each tank were sampled to measure the immune parameters namely total leucocyte count and phagocytosis activity of phagocyte cells. Research results showed that after three weeks of feeding, total leucocyte and phagocytosis activity of fish fed pellet supplemented with yeast cells were significantly different as compared to those of control fish.  The highest total leucocyte and phagocytosis activity were achieved in fish fed pellet supplemented with 10 g of yeast cells/kg pellet.  It was concluded that the use of yeast cells for three weeks could enhance the nonspecific immune response of nile tilapia. Keywords: Saccharomyces cerreviciae, nonspecific immune response, total leucocyte count, phagocytosis activity, nile tilapia, Oreochromis niloticus
Respon imun krustase Manoppo, Henky; Kolopita, Magdalena E.F
e-Journal BUDIDAYA PERAIRAN Vol 2, No 2 (2014)
Publisher : e-Journal BUDIDAYA PERAIRAN

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Crustacean does not have adaptive immune system and mostly depends on innate or nonspecific immune response. This system can recognize and destroy foreign materials including pathogen.  The nonspecific immune responses of crustacean consist of cellular and humoral responses.  In cellular response, hemocyte plays an important role in body defenses to pathogen including virus, bacteria, fungi, and parasites. In humoral response, recognition of pathogen is mediated by protein and enzyme such as β-1,3-glucan-binding protein (BGBP),  lipopolysaccharide-binding protein (LPS-BP), peptidoglycan recognition protein, phenoloxydase enzime. Keywords: crustacean, nonspecific immune response, cellular responses, humoral response, protein, enzime
Peningkatan Pertumbuhan Ikan Nila (Oreochromis niloticus) Melalui Pemberian Immunostimulan Ragi Roti (Saccharomyces cereviciae) Bugis, Achmad A; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 2, No 3 (2014)
Publisher : e-Journal BUDIDAYA PERAIRAN

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This research aimed to examine the effect of baker’s yeast as immunostimulant on growth of nile tilapia and to find out a proper dose of baker’s yeast that promoted optimal growth of fish. Juveniles with an average weight of 31.83 g were obtained from stock available at Aquaculture Study Program of the Faculty of Fisheries and Marine Sciences.  This research was conducted at the experimental ponds located at the Faculty of Fisheries and Marine Sciences for three months. During the experiment, fish was fed with pellet supplemented with baker’s yeast as treatment for four weeks as much as 4%/bw/day, twice daily.  The doses of baker’s yeast consisted of 0, 10, 20, 30, and 40 g/kg pellet. Fish growth including absolute and relative growth was measured at two weeks interval.  Research results showed that  after two weeks of feeding, supplementation of baker’s yeast did not affect the growth of fish but after four weeks of feeding, supplementation of baker’s yeast significantly increased fish growth. Fish fed pellet supplemented with 10 g baker’s yeast displayed the highest growth. As conclusion, supplementation of baker’s yeast in fish food could enchance the growth of fish.   Keywords:  Oreochromis niloticus, immunostimulant, baker’s yeast, growth
Kelangsungan Hidup Larva Ikan Nila yang Berasal Dari Induk yang Diberi Pakan Berimunostimulan Maleko, Ayu; Sinjal, Hengky J; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 2, No 3 (2014)
Publisher : e-Journal BUDIDAYA PERAIRAN

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The objective of this research was to evaluate the effect of feed supplemented with baker’s yeast on survival rate of nile tilapia larvae and to determine the effective dose of baker’s yeast that could optimally improve larval quality.  The research was conducted at Pathology and Clinic of Fish Disease from June – September 2014.  Fish as brood stock candidates was cultivated at five concrete tanks (2x1x1 m3) at a density of 30 fish per tank.  Each tank was equipped with water inlet, outlet and water pump for recirculating and aerating.  After seven days of adaptation, fish was fed pellet supplemented with baker’s yeast at five different doses (0, 10, 20, 30, 40 g/kg food) for four consecutive weeks at 4%/bb/day, twice daily.  Larva was captured with seser and moved into five glass aquaria with density of 20 larvae/aquarium and reared for two weeks.  Data collected was survival rate of larvae achieved until the end of larval rearing.  Research result showed that larvae produced by brood stock fed food supplemented with 10 g baker’s yeast per kg food had the highest survival rate namely 60%.  As conclusion, supplementation of baker’s yeast into brood stock feed might increase survival rate of larvae.   Keywords:  nile tilapia, baker’s yeast, larvae, survival rate